• Journal of Animal Environmental Science
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• v.1 no.1
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• pp.65-75
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• 1995

This experiment was carried out to the effects of population density and C/N ratios of feed on the growth, reproductive effciency and cast producation of the earthworm(Eisenia foetida). The population densities of 50, 100, 150, 200 and 250 individuals of the earthworm fed with different C/N ratios of 25, 35, 45 and 55 cow manures were studied in rearing box($6,400cm^3$), and at the fertility stage during a period of 60 days. The results were summarized as follows; The survial rate(SR), increasing rate(IR), reproductive efficiency(RE) and cast production of the earthworms showed highest values in C/N ratio of 25. These results may indicate that C/N ratio of 25 is a very favourable feed for the growth of the earthworms. The survial rate(SR) indicated significant positive correlation with reproductive efficiency(RE) in different C/N ratios of feeds. The survial rate(SR) showed highest values in population densities of $50{\sim}100$ worms/$6,400cm^3(64.0{\sim}128.0cm^3/worm$). On the contrary, increasing rate(IR) tended to decreased with the increased population densities. The survival rate(SR) indicated significant negative correlation with reproductive efficiency(RE) in different population densities of the earthworms. The cast production estimated were $31.6mg{\sim}67.4mg/day/worm$ grown in optimum population densities($50{\sim}100\;worms/6,400cm^3$). The earthworm casting are an excellent soil conditioning material or organic fertilizer sources with a high chemical composition and their physical properties.

• Journal of Aquaculture
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• v.11 no.2
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• pp.125-132
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• 1998

The results of experimental culture for Koran mitten crab, Eriocheir japonicus at different outdoor culture methods such as rice field, pond, and vinyl house from July 21, 1994 to October 23, 1995 were summaried as fellows. 1. The water temperature was 2${\circ}C$~32${\circ}C$ during the culture periods from July 21, 1994 to October 23, 1995, and it ranged from 15${\circ}C$ to 30${\circ}C$ during the terms showing the intake habit of feed with these temperature maintained for 281 days of 460days of the total cultur periods. 2. The gain weight of Korean mitten crabs was 1,711.3g at the rice field, and 1,565.4g at the pond, and 1.220.0g at the vinyl house. The survial rate was 22.7% at the pond, and 18.7% at the rice field, and 18.3% at the vinyl house with the average survial rate of 19.9%. The daily growth rate of cauapace width was 0.64% at the rice field, and 0.61% at the pond, and 0.59% at the vinyl house. And the gain weight and the daily growth rate were the best at the rice field. 3. When the culture was finished, the frequency of carapace width of 4.0~4.9cm was 57.1% at the rice field, and 33.3% at the vinyl house. Therefore, culture at the rice field for the cultur of mitten card was considered to be the best outdoor culture method from the point of view in the fast growth and polyculture feasibility with paddy rice.

• The Journal of the Korean dental association
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• v.49 no.5
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• pp.279-285
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• 2011

Currently there is no dental ceramic material can be used in all dental situations need to be restored. However, in view of recent clinical reports, the most viable alternative is zirconia ceramic. Clinical success of dental zirconia restorations strongly depends on proper selection of materials, accurate laboratory procedure and final cementation, which can be achievable with the correct understanding of zirconia. As dental materials, zirconia ceramics have a very bright future, because they are being used increasingly in the anterior region as implant fixtures, as well as crown and bridge restorations and implant abutments. Many dental ceramics showing poor clinical performance have been gone from the dental market. However, in terms of outstanding mechanical properties and esthetic nature, new dental materials can replace zirconia ceramics will not be available in the foreseeable future.

• Journal of Sericultural and Entomological Science
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• v.27 no.2
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• pp.1-6
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• 1985

To study the non-sprouting causes of mulberry saplings, 8 treatments, borax + lime + compost, borax, lime, Jamsi fertilizer No. 1, heavy nitrogen application, branch harvest, heavy harvest and no treatment were applied on the nursery garden. The tract used as the paddy field before sapling produced was very poor in the soil chemical and physical properties. The results were as follows; 1. The survial rates of saplings were higher than 98% in the treatments of borax, lime, Jamsi fertilizer No. 1, whereas were higher than 98% in the treatments of borax, lime, Jamsi fertilizer No. 1, whereas heavy nitrogen 59% and heavy harvest 71%. 2. The survial rate was showed deeper relationship with the chemical concentration in stem cortex than in root cortex, especially the content of B, Cu and Zn. 3. The nitrogen content in stem cortex was much higher in the heavy nitrogen treatment by 0.89%, where as 0.6∼0.7% in the other treatments. However, treatments of the heavy harvest and the branch harvest were around 5%. 4. In order to decrease the non-sprouting sapling rate, nursery garden should be good in soil chemical and physical properties. Harvest from saplings and heavy nitrogen application should be prevented and lime or Jamsi fertilizer should be applied to saplings.

• Journal of Embryo Transfer
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• v.23 no.1
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• pp.19-24
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• 2008

The purpose of this study was to compare the efficiency of slow freezing with that of vitrification method for the cryopreservation of human embryos. Human embryos were derived from in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) and the mixed solution of propanedial (1.5, 1.0, 0.5M PROH) and sucrose (0.1M), ethylene glycol (7.5, 15%), dimethyl sulfoxide (7.5, 15% DMSO), sucrose (0.5, 1.0M) and SPS (Serum Protein Substitute) was used for a cryoprotectant for slow freezing and vitrification solution, respectively. Rates of recovery after thawing, morphological normality, post-thaw viability, arrest, morphological abnormality and preimplantation development were compared between two protocols. After freezing-thawing, recovery and survial rate of slow freezing was (88.6% and 73.4%), whereas vitrification was (99.2% and 96.2%) (p<0.05). The arrest rate of slow freezing was significantly lower compared with those of vitrification(8.7% vs 29.9%) (p<0.05). Preimplantation development to the 2-cell (83.8% vs 67.7%), 4-cell (69.0% vs 47.2%) and 8-cell (62.4% vs 37.8%) stages 24, 48 and 72 h after thawing, respectively, were higher in the slow freezing than the vitrification. After slow freezing and vitrification of human embryo at 2-8cell stage, the rate of recovery rate, survival rate and partial damage rate were 92.0% vs 100%, 80.4% vs 96.2% and 52.2% vs 19.0%, respectively. And partial damage rate was significantly lower than those of slow freezing method (p<0.05). These results demonstrate that a slow freezing using PROH is more efficient than a vitrification for cryopreserving the human zygotes, although the vitrification yielded better recovery, survival and partial damage of frozen-thawed 2-8 cell stage embryos than slow freezing method.

• Journal of Sericultural and Entomological Science
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• v.31 no.1
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• pp.12-19
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• 1989

Experiments were conducted to investigate the optimum season for mulberry graftages harvesting before the period of natural leaf fall in autumn. Harvesting season was examined at 10 days intervals from September 30 to November 20, 1985 and 1986, at nine sites in eight provinces. The results were : 1. Diameter of graftages increased until October 30. 2. Early harvest of graftages decreased survived rate, number of shoots, and total shoot length the next spring. The October 30 harvest yielded the best growth the following spring. 3. Harvests after October 20 allowed for good survial of mulberry shoots and sprouting the following spring. 4. Total N and P in plants increased with late harvests, K decreased, and Ca content was not affected by harvest date. 5. Total N, P, and Ca were higher in stem cortex than root bark, while K appeared to be equally distributed between root and shoot bark.

• Biomolecules & Therapeutics
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• v.8 no.3
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• pp.255-261
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• 2000

WooHwangChungSimWon is a traditional prescription for treating with hypertension, arteriosclerosis, coma, and stroke in China, Korea, and Japan. In the new presciption of WooHwangChungSimWon, the covet is substituted for the musk, the major component of WooHwangChungSimwon, because of the prohibition law about the musk. We have made a comparative study of the effects on the ischemic damage between the musk containing and civet containing WooHwangChungSimWon. In order to investigate the effects of WooHwangChungSimWon on the ischemic damage, each samples were administrated for 12 days, ischemia was induced for 10 minutes at 7th day, and immunohistochemistry was performed in the region of hippocampus of mongolian gerbils. According to the result of immunohistochemistry, the survival rates of neuroal cells in the hippocampal CA1 region are 37.8% in the high dose of musk containing WooHwangChungSimWon (HM-WHCSW) administrated group, 27.8% in low dose of civet containing WooHwangChungSimWon (LC-WHCSW), and 35.5% in high dose of civet containing WooHwangChungSimWon (HC-WHCSW) administrated group. These survial rates were significantly different from the survival rate of sham control group (14.4%). The results suggest that all the samples except the low dose of musk containing WooHwangChungSimWon (LM-WHCSW) have protective or preventive effects on cerebral ischemia.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.4
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• pp.238-244
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• 1995

The objective of this study is to obtain the basic data for investigating the effects of organic reserves on winter survial or regrowth yield. Forage rape(Brassica napus L.) was sown on Sep. 1, 1994. Experimental plots were divided into three replicates under 6 different cutting dates(l0 days interval from Oct. 15 to Dec. 4). Field-grown palnts were sampled on the each cutting date and on the wintering period (Jan. 16) to analyze the nitrogen and non-structural cahohydrate reserves. The rate of winter survival and regrowth yield were also measured in the spring of next year. On the before wintering, dry matter yields were 152, 274, 500, 718, 776 and 981 kg/lOa, respectively, from the cutting date on Oct. 15, Oct. 25, Nov. 4, Nov. 14, Nov. 24, and Dec. 4. Cmde protein yield significantly increased as cutting date was later until Nov. 14, thereafter a significant increase did not occured. Nitrogen and starch contents per plant significnatly increased as the cutting date was later. The increasing rate of starch was greatly higher than that of nitrogen. On the wintering period, nitrogen reserves in mts were 85.3, 68.8, 47.6, 28.3, 44.3, and 55.3 mglplant, and starch reserve were 11 1.3, 75.3, 39.3, 19.6, 26.4 and 34.6 mglplant, respectively, in the plots cut on Oct. 15, Oct. 25, Nov. 4, Nov. 14, Nov. 24, and Dec. 4. It showed that carbohydrate reserves were much highly utilized than nitrogen reserves during wintering period. The rates of winter survival were 91, 83, 46, 22, 35 and 43% and regrowth yields were 692, 545, 316, 84, 127 and 140 kgD.M/lOa, respectively, in each plots. The highly significant correlation (p<0.01) between the level of organic reserves and the rate of winter survival or regrowth yield were obtained.

• Journal of the Korean Institute of Landscape Architecture
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• v.29 no.4
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• pp.82-90
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• 2001

This study was carried out to reveal optimum conditions for in vitro propagation of 3 Korean native azalea species, Rhododendron mucronulatum, R. yedonese var. poukhanense, and R. shlippenbachii, which are useful for landscape proposes. Seeds and meristems from three azalea species were cultured on 1/2MS, Hyponex, and Anderson media containing growth of regulators benzyladenine(BA) and 2-isopentenyadenosine(2ip). The results were as follows. 1. In the culture of R. schlippenbachii and R. mucronulatum seeds, in vitro seedlings germinated and grew well on he 1/2MS and Anderson media, while R. yedoense var. poukhanense on Hyponex media containing 6.0mg/$\ell$ 2ip. 2. When the meristems of R. mucronulatum were cultured on Andeson media containing 9.0mg/$\ell$ 2ip, the survival rate of meristems was 23.0% in 6 weeks after culture, and the survival rate of R. schlippenbachii was 46.0% o nthe same media containing 12.0mg/$\ell$ 2ip. The survial rate of R. yedoense var. poukhanense was 92.0% onHyponex media containing 0.5mg/$\ell$ BA and 9.0mg/$\ell$ 2ip. When the meristems of R. mucronulatum and R. yedoense var. poukhanense were cultured on Hyponex media containing 12.0mg/$\ell$ 2ip, they showed the most excellent growth. R. schlippenbachii grew well on Anderson media containing 9.0mg/$\ell$ 2ip. When in vitro shoots of R. yedoense var. poukhanense were subcultured to solid medium, they grew well in shoot growth on Hyponex media containing 6.0mg/$\ell$ 2ip.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.2
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• pp.164-170
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• 2000

This study was carried out to investigate the effect of photosynthetic bacteria to chlorella or ${\omega}-yeast$ on Browth of the rotifer and its dietary value for flounder, Paralicbthys oliraceus. The rotifer fed the chlorella (200,000 cells/ind./day) with the addition of 20 times the photosynthetic bacteria of the chlorella concentration showed the highest growth. But the specific growth rate of 100,000 chlorella/ind./day with the addition of 30 times the photosynthetic bacteria was the most economical feeding regime for mass culture of the rotifer. The rotifer frd ${\omega}-yeast$ with 200,000 cells/ind./days with the addition of 20 times the photosynthetic bacteria of the chlorella conecentration showed the highest growth. Growth and survial rate of the larvae of Paralichithys oiivaceus fed the rotifer reared on both chlorella and ${\omega}-yeast$ with the addition of photosynthetic bacteria were higher than those without the bacteria, and the chlorella had better dietary value than the ${\omega}-yeast$ for the larvae. The larvae fed the rotifer which was cultured with the chlorella of 200,000 cells/ind./day and the photosynthetic bacteria of $4{\times}10^6$ cells/ind./day showed the highest survial rate and growth. The larvae reared with the addition of the photosynthetic bacteris had higher total lipid and the total content of EPA and DHA than those reared without the bacteria. The larvae fed the enriched artemia nauplius with the photosynthetic bacteria also showed higher suurval rate and growth than those fed the nauplius without the enrichment. The optimum enrichment concentration of the photosynthetic bacteria for artemia nauplius was $2{\times}1^7\;cells/ml$. The addition of the photosynthetic bacteria to the chlorella and the ${\omega}-yeast$ was effective to growth of the rotifer and dietary value for the flounder larvae. However, an excessive addition of the bacteria decreased both the growth of the rotifer and the dietary of the larvae.