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Presence of Leukemia-maintaining Cells in Differentiation-resistant Fraction of K562 Chronic Myelogenous Leukemia (만성 골수성 백혈병 K562세포의 분화 내성 분획에서 백혈병 유지 세포의 동정)

  • Lee, Hong-Rae;Kim, Mi-Ju;Ha, Gahee;Kim, So-Jung;Kim, Sun-Hee;Kang, Chi-Dug
    • Journal of Life Science
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    • v.23 no.2
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    • pp.197-206
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    • 2013
  • The present study investigated whether leukemia-maintaining cells reside in a differentiation-resistant fraction using a megakaryocytic differentiation model of K562 cells. Treatment with phorbol-12-myristate-13-acetate (PMA) significantly inhibited the colony-forming efficiency of the K562 cells. At a PMA concentration of 1 nM or higher, colony was not formed, but approximately 40% of K562 cells still survived in soft agar. Approximately 70% of colony-forming cells that were isolated following the removal of PMA after exposure to the agent were differentiated after treatment with 10 nM PMA for 3 days. The differentiation rate of the colony-forming cells was gradually increased and reached about 90% 6 weeks after colony isolation, which was comparable to the level of a PMA-treated K562 control. Meanwhile, imatinib-resistant variants from the K562 cells, including K562/R1, K562/R2, and K562/R3 cells, did not show any colony-forming activity, and most imatinib-resistant variants were CD44 positive. After 4 months of culture in drug-free medium, the surface level of CD44 was decreased in comparison with primary imatinib-resistant variants, and a few colonies were formed from K562/R3 cells. In these cells, Bcr-Abl, which was lost in the imatinib-resistant variants, was re-expressed, and the original phenotypes of the K562 cells were partially recovered. These results suggest that leukemia-maintaining cells might reside in a differentiation-resistant population. Differentiation therapy to eliminate leukemia-maintaining cells could be a successful treatment for leukemia if the leukemia-maintaining cells were exposed to a differentiation inducer for a long time and at a high dose.

Can We Hear the Shape of a Noise Source\ulcorner (소음원의 모양을 들어서 상상할 수 있을까\ulcorner)

  • Kim, Yang-Hann
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.14 no.7
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    • pp.586-603
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    • 2004
  • One of the subtle problems that make noise control difficult for engineers is “the invisibility of noise or sound.” The visual image of noise often helps to determine an appropriate means for noise control. There have been many attempts to fulfill this rather challenging objective. Theoretical or numerical means to visualize the sound field have been attempted and as a result, a great deal of progress has been accomplished, for example in the field of visualization of turbulent noise. However, most of the numerical methods are not quite ready to be applied practically to noise control issues. In the meantime, fast progress has made it possible instrumentally by using multiple microphones and fast signal processing systems, although these systems are not perfect but are useful. The state of the art system is recently available but still has many problematic issues : for example, how we can implement the visualized noise field. The constructed noise or sound picture always consists of bias and random errors, and consequently it is often difficult to determine the origin of the noise and the spatial shape of noise, as highlighted in the title. The first part of this paper introduces a brief history, which is associated with “sound visualization,” from Leonardo da Vinci's famous drawing on vortex street (Fig. 1) to modern acoustic holography and what has been accomplished by a line or surface array. The second part introduces the difficulties and the recent studies. These include de-Dopplerization and do-reverberation methods. The former is essential for visualizing a moving noise source, such as cars or trains. The latter relates to what produces noise in a room or closed space. Another mar issue associated this sound/noise visualization is whether or not Ivecan distinguish mutual dependence of noise in space : for example, we are asked to answer the question, “Can we see two birds singing or one bird with two beaks?"

A Study on Real-Time Defect Detection Using Ultrasound Excited Thermography (초음파 서모그라피를 이용한 실시간 결함 검출에 대한 연구)

  • Cho, Jai-Wan;Seo, Yong-Chil;Jung, Seung-Ho;Jung, Hyun-Kyu;Kim, Seung-Ho
    • Journal of the Korean Society for Nondestructive Testing
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    • v.26 no.4
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    • pp.211-219
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    • 2006
  • The UET(ultrasound excited thermography) for the ,eat-time diagnostics of the object employs an infrared camera to image defects of the surface and subsurface which are locally heated using high-frequency putted ultrasonic excitation. The dissipation of high-power ultrasonic energy around the feces of the defects causes an increase In temperature. The defect's image appears as a hot spot (bright IR source) within a dark background field. The UET for nondestructive diagnostic and evaluation is based on the image analysis of the hot spot as a local response to ultrasonic excited heat deposition. In this paper the applicability of VET for fast imaging of defect is described. The ultrasonic energy is injected into the sample through a transducer in the vertical and horizontal directions respectively. The voltage applied to the transducer is measured by digital oscilloscope, and the waveform are compared. Measurements were performed on four kinds of materials: SUS fatigue crack specimen(thickness 14mm), PCB plate(1.8 mm), CFRP plate(3 mm) and Inconel 600 plate (1 mm). A high power ultrasonic energy with pulse durations of 250ms Is injected into the samples in the horizontal and vertical directions respectively The obtained experimental result reveals that the dissipation loss of the ultrasonic energy In the vertical injection is less than that in the horizontal direction. In the cafe or PCB, CFRP, the size of hot spot in the vortical injection if larger than that in horizontal direction. Duration time of the hot spot in the vertical direction is three times as long as that in the horizontal direction. In the case of Inconel 600 plate and SUS sample, the hot spot in the horizontal injection was detected faster than that in the vertical direction

Effect of an Offshore Fish Culture System on the Benthic Polychaete Community (외해가두리 양식이 저서다모류군집에 미치는 영향)

  • Jung, Rae-Hong;Yoon, Sang-Pil;Kim, Youn-Jung;Lee, Won-Chan;Hong, Sok Jin;Park, Sung-Eun;Oh, Hyung Taik
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.18 no.4
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    • pp.195-205
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    • 2013
  • Excessive input of organic matters from fish cage farming has been considered as one of the major factors disturbing benthic ecosystem, especially in semi-enclosed coastal waters. Recently offshore aquaculture in the vicinity of Jeju-do has been introduced to minimize that kind of negative impact. This study was conducted to investigate the ecological impacts of offshore aquaculture on the macrobenthic polychaete communities. A total of ten sampling works were carried out for 28 months, spanning from 10 days after starting giving feed to 3 months after stopping giving feed. During the study period, mean current velocity was quite strong with the range of 50 cm/s to 70 cm/s. TOC of surface sediment was constantly low. Significant changes in polychaete community were detected just three months after starting giving feed, which were the increase of the number of species and density at all stations. Up to 18 months after the start of farming, the amount of feed provided played an important role in the fluctuation of the number of species and density, especially at 0 m and 10 m stations. After reducing the amount of feed provided, dominance of some opportunistic species within 10 m distance from fish cages still lasted to the end of aquaculture. However, opportunistic species disappeared 3 months after the end of farming, which indicated the sign of recovery from the disturbance. From these results, the amount of food input and the period of cultivation were critical factors disturbing polychaete community and ensuing changes in this offshore and oligotrophic waters as well. In addition, study on the changes of polychaete community structure before and after fish farming showed more detailed changes in benthic ecological state than geochemical approach did.

The Non-Destructive Determination of Heavy Metals in Welding Fume by EDXRF (EDXRF에 의한 용접흄 중의 중금속의 비파괴 정량)

  • Park, Seunghyun;Jeong, Jee Yeon;Ryoo, Jang Jin;Lee, Naroo;Yu, Il Je;Song, Kyung Seuk;Lee, Yong Hag;Han, Jeong Hee;Kim, Sung Jin;Park, Jung sun;Chung, Ho Keun
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.11 no.3
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    • pp.229-234
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    • 2001
  • The EDXRF(Energy Dispersive X-ray Fluorescence Spectrometer) technique was applied to the determination of heavy metals in welding fume. The EDXRF method designed in this study was a non-destructive analysis method. Samples were analyzed directly by EDXRF without any pre-treatment such as digestion and dilution. The samples used to evaluate this method were laboratory samples exposed in a chamber connected with a welding fume generator. The samples were first analyzed using a non-destructive EDXRF method. The samples subsequently were analyzed using AAS method to verify accuray of the EDXRF method. The purpose of this study was to evaluate the possibility of the non-destructive analysis of heavy metals in welding fume by EDXRF. The results of this study were as follow: 1.When the samples were collected under the open-face sampling condition, a surface distribution of welding fume particles on sample filters was uniform, which made non-destructive analysis possible. 2. The method was statistically evaluated according to the NIOSH(National Institute for Occupational Safety and Health) and HSE(Health and Safety Executive) method. 3. The overall precision of the EDXRF method Was calculated at 3.45 % for Cr, 2.57 % for Fe and 3.78 % for Mn as relative standard deviation(RSD), respectively. The limits of detection were calculated at $0.46{\mu}g$/sample for Cr, $0.20{\mu}g$/sample for Fe and $1.14{\mu}g$/sample for Mn, respectively. 4. A comparison between the results of Cr, Fe, Mn analyzed by EDXRF and AAS was made in order to assess the accuracy of EDXRF method. The correlation coefficient between the results of EDXRF and AAS was 0.9985 for Cr, 0.9995 for Fe and 0.9982 for Mn, respectively. The overall uncertainty was determined to be ${\pm}12.31%$, 8.64 % and 11.91 % for Cr, Fe and Mn, respectively. In conclusion, this study showed that Cr, Fe, Mn in welding fume were successfully analyzed by the EDXRF without any sample pre-treatment such as digestion and dilution and a good correlation between the results of EDXRF and AAS was obtained. It was thus possible to use the EDXRF technique as an analysis method of working environment samples. The EDXRF method was an efficient method in a non-destructive analysis of heavy metals in welding fume.

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A Study on Oxygen Reduction Reaction of PtM Electrocatalysts Synthesized by a Modified Polyol Process (수정된 폴리올 방법을 적용하여 합성한 PtM 촉매들의 산소환원반응성 연구)

  • Yang, Jongwon;Hyun, Kyuwhan;Chu, Cheunho;Kwon, Yongchai
    • Applied Chemistry for Engineering
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    • v.25 no.1
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    • pp.78-83
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    • 2014
  • In this research, we evaluated the performance and characteristics of carbon supported PtM (M = Ni and Y) alloy catalysts (PtM/Cs) synthesized by a modified polyol method. With the PtM/Cs employed as a catalyst for the oxygen reduction reaction (ORR) of cathodes in proton exchange membrane fuel cells (PEMFCs), their catalytic and ORR activities and electrical performance were investigated and compared with those of commercial Pt/C. Their particle sizes, particle distributions and electrochemically active surface areas (EAS) were measured by TEM and cyclic voltammetry (CV), while their ORR activity and electrical performance were explored using linear sweeping voltammetries with rotating disk electrodes and rotating ring-disk electrodes as well as PEMFC single cell tests. TEM and CV measurements show that PtM/Cs have the compatible particle size and EAS with Pt/C. When it comes to ORR activity, PtM/C showed the equivalent or better half-wave potential, kinetic current density, transferred electron number per oxygen molecule and $H_2O_2$ production(%) to or than commerical Pt/C. Based on results gained by the three electrode tests, when the PEMFC single cell tests were carried out, the current density measured at 0.6 V and maximum power density of PEMFC single cell adopting PtM/C catalysts were better than those adopting Pt/C catalyst. It is therefore concluded that PtM/C catalysts synthesized by modified polyol can result in the equivalent or better ORR catalytic capability and PEMFC performance to or than commercial Pt/C catalyst.

Combined Effects of Filter-feeding Bivalve and Zooplankton on the Growth Inhibition of Cyanobacterium Microcystis aeruginosa (남세균 제어를 위한 동물플랑크톤(Daphnia magna)과 패류(Unio douglasiae)의 단독 및 혼합적용)

  • Kim, Nan-Young;Park, Myung-Hwan;Hwang, Su-Ok;Kim, Baik-Ho;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.48 no.2
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    • pp.108-114
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    • 2015
  • Single - and combined effects of a domestic freshwater bivalve Unio douglasiae (7.6~8.6 cm in shell length) and zooplankton Daphnia magna (1~2 mm in body size) were examined to understand whether they inhibit the growth of harmful cyanobacterial bloom (i.e. Microcystis aeruginosa) in a eutrophic lake. The experiments were triplicated with twelve glass aquaria (40 L in volume); three aquaria without mussel and zooplankton, served as a control, three zooplankton aquaria (Z, density=40 indiv. $L^{-1}$), three mussel aquaria (M, density=0.5 indiv. $L^{-1}$), and three mussel plus zooplankton aquarium (ZM, density=40 indiv.Z $L^{-1}$ plus 0.5 indiv.M/L), respectively. Algal growth inhibition (%) calculated as a difference in the concentration of chlorophyll-a (Chl-a) before and after treatment. Chl-a in all aquaria decreased with the time, while a greatest algal inhibition was seen in the ZM aquaria. After 24 hrs of incubation, Chl-a concentration at the mid-depth (ca. 15 cm) in ZM aquaria reduced by 90.8% of the control, while 63.2% and 79.8% in Z and M aquaria, respectively. Interestingly, during the same period, the surface Chl-a was diminished by 51.9% and 65.4% relative to the control in Z and ZM aquaria, while 27.4% of initial concentration decreased in M aquarium, respectively. These results suggest that 1) this domestic freshwater filter-feeding bivalve plays a significant role in the control of cyanobacterial bloom (M. aeruginosa), and 2) the combination with zooplankton and mussel has a synergistic effect to diminish them, compared to the single treatment of zooplankton and mussel.

Study on Effect of Convection Current Aeration System on Mixing Characteristics and Water Quality of Reservoir (대류식 순환장치의 저수지수체 유동특성 및 수질영향)

  • Lee, Yo-Sang;Lee, Kwang-Man;Koh, Deok-Koo;Yum, Kyung-Taek
    • Korean Journal of Ecology and Environment
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    • v.42 no.1
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    • pp.85-94
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    • 2009
  • This study examines the operational effectiveness of a Convection Current Aeration System (CCAS) in reservoir. CCAS was run from June, 2008 when the thermocline begun forming in the reservoir. This paper reviews the influence of stratification, dissolved oxygen dynamics and temperature in the lake's natural state from June to October 2008. The survey was done on a week basis. Upwelling flow effects a radius of $7{\sim}10m$ at a surface directly and was irrelevant to the strength of thermocline. On the other hand, it was affected the number of working days, and strength of thermocline at vertical profiles of the reservoir. Longer CCAS run, the deeper was the vertical direct flow area. However it didn't break the thermocline during summer season of 2008. The operating efficiency of the CCAS in the reservoir depends on hydraulics and meteological conditions. Computational Fluid Dynamics (CFD) is a very useful tool for evaluating the operating efficiency of fluid dynamics. The geometry for CFD simulation consists of a cylindrical vessel 25 m radius and 40 m height. The CCAS is located in center of domain. The non-uniform tetrahedral meshes had a bulk of the geometry. The meshes ranged from the coarse to the very fine. This is attributed to the cold water flowing into the downcomer and rising, creating a horizontal flow to the top of the CCAS. The result of CFD demonstrate a closer agreement with surveyed data for temperature and flow velocity. Theoretical dispersion volume were calculated at 8m depth, 120 m diameter working for 30 days and 10 m depth, 130 m diameter working for 50 days.

Research Trend of Biomass-Derived Engineering Plastics (바이오매스 기반 엔지니어링 플라스틱 연구 동향)

  • Jeon, Hyeonyeol;Koo, Jun Mo;Park, Seul-A;Kim, Seon-Mi;Jegal, Jonggeon;Cha, Hyun Gil;Oh, Dongyeop X.;Hwang, Sung Yeon;Park, Jeyoung
    • Applied Chemistry for Engineering
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    • v.31 no.2
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    • pp.115-124
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    • 2020
  • Sustainable plastics can be mainly categorized into (1) biodegradable plastics decomposed into water and carbon dioxide after use, and (2) biomass-derived plastics possessing the carbon neutrality by utilizing raw materials converted from atmospheric carbon dioxide to biomass. Recently, biomass-derived engineering plastics (EP) and natural nanofiber-reinforced nanocomposites are emerging as a new direction of the industry. In addition to the eco-friendliness of natural resources, these materials are competitive over petroleum-based plastics in the high value-added plastics market. Polyesters and polycarbonates synthesized from isosorbide and 2,5-furandicarboxylic acid, which are representative biomass-derived monomers, are at the forefront of industrialization due to their higher transparency, mechanical properties, thermal stability, and gas barrier properties. Moreover, isosorbide has potential to be applied to super EP material with continuous service temperature over 150 ℃. In situ polymerization utilizing surface hydrophilicity and multi-functionality of natural nanofibers such as nanocellulose and nanochitin achieves remarkable improvements of mechanical properties with the minimal dose of nanofillers. Biomass-derived tough-plastics covered in this review are expected to replace petroleum-based plastics by satisfying the carbon neutrality required by the environment, the high functionality by the consumer, and the accessibility by the industry.

A Study on Physical Properties About Velvet Antler of the Elk(Cerus canadensis) (엘크 녹용의 물성 특성에 관한 연구)

  • Sung, S.H.;Han, J.H.;Kim, Y.M.;Kim, B.K.
    • Journal of Animal Environmental Science
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    • v.13 no.3
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    • pp.201-210
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    • 2007
  • In this study, physical properties of Elk antler was investigated to develop the optimum drying and packaging methods for improving the antler quality as well as deal with diversify of demand. After the antler was sliced with 5 mm thickness, and the compressive, shear, and tensile stresses were measured at the center and velvet parts of pre-dried and dried antlers after the contained water rate of the dried antlers was maintained below 10%. The results are as follows. 1. Considering the center of pre-dried antlers, the compressive stresses were $60.73\;g/mm^2$, $145.65\;g/mm^2$, and $260.97\;g/mm^2$, respectively at the upper, middle, and lower parts while $70.67\;g/mm^2$, $811.90\;g/mm^2$, $3,235.52\;g/mm^2$, respectively for velvet layer. Considering the center of dried antlers, the compressive stresses were $190.43\;g/mm^2$, $445.81\;g/mm^2$, and $705.86\;g/mm^2$, respectively at the upper, middle, and lower parts while $734.01\;g/mm^2$, $1,238.40\;g/mm^2$, $4,134.03\;g/mm^2$, respectively for velvet layer. 2. For the pre-dried, the shear stresses were $50.24\;g/mm^2$, $294.44\;g/mm^2$, and $423.47\;g/mm^2$, respectively, and $124.14\;g/mm^2$, $367.69\;g/mm^2$, and $425.86\;g/mm^2$, respectively for the dried antlers. 3. The tensile stresses were $13.59\;g/mm^2$, $62.85\;g/mm^2$, and $112.07\;g/mm^2$, respectively for the pre-dried and $77.24\;g/mm^2$, $175.87\;g/mm^2$, and $184.06\;g/mm^2$, respectively for the dried antlers. 4. In the case of drying antlers, the physical characteristics of the antlers was. changed such as moisture evaporation, contraction, and surface hardening. For the center part, the changes of the physical characteristics were more significant at the lower part while at the upper part for the velvet layer. 5. The stress changes of Elk antlers was shown very remarkably according to growth point. Moreover, the stress was clearly higher at velvet layer part to the center part, base parts compared to the upper parts.

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