• Journal of Ginseng Research
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• 제34권4호
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• pp.296-303
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• 2010

A peroxiredoxin cDNA (PgPrx) was isolated and characterized from the leaves of Panax ginseng. The cDNA is 716 nucleotides long and has an open reading frame of 489 base pairs with a deduced amino acid sequence of 162 residues. The calculated molecular mass of the mature protein is approximately 17.4 kDa with a predicted isoelectric point of 5.37. A GenBank BlastX search revealed that the deduced amino acid sequence of PgPrx shares a high degree homology with type II peroxiredoxin (Prx) proteins in other plants. The PgPrx gene was highly expressed in leaves, and expressed at a low level in the stem. To analyze the gene expression of PgPrx in response to various abiotic stresses, we utilized real-time quantitative RT-PCR. Our results reveal that PgPrx expression is induced by ultraviolet irradiation, low temperature, and salt. The induction of PgPrx in response to abiotic stimuli suggests that ginseng Prx may function to protect the host against environmental stresses.

• Journal of Ginseng Research
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• 제34권1호
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• pp.17-22
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• 2010

Sesquiterpenes are found naturally in plants and insects as defensive agents or pheromones. They are produced in the cytosolic acetate/mevalonate pathway for isoprenoid biosynthesis. The inducible sesquiterpene synthases (STS), which are responsible for the transformation of the precursor farnesyl diphosphate, appear to generate very few olefinic products that are converted to biologically active metabolites. In this study, we isolated the STS gene from Panax ginseng C.A. Meyer, designated PgSTS, and investigated the correlation between its expression and various abiotic stresses using real-time PCR. PgSTS cDNA was observed to be 1,883 nucleotides long with an open reading frame of 1,707 bp, encoding a protein of 568 amino acids. The molecular mass of the mature protein was determined to be 65.5 kDa, with a predicted isoelectric point of 5.98. A GenBank BlastX search revealed the deduced amino acid sequence of PgSTS to be homologous to STS from other plants, with the highest similarity to an STS from Lycopersicon hirsutum (55% identity, 51% similarity). Real-time PCR analysis showed that different abiotic stresses triggered significant induction of PgSTS expression at different time points.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 추계학술발표회
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• pp.154-155
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• 2011

• Journal of Ginseng Research
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• 제5권2호
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• pp.155-162
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• 1981

This study was aimed at elucidating the composition and color in ginseng extracts during the processing and the long periods of the storage. The types of sugar were determined by using HPLC. In the model study with the fresh ginseng extracts stored at the elevated temperatures between 70-100$^{\circ}C$ for 24-96 hrs, it was shown an overall increase in the concentration of fructose and the overall reduction in the concentrations of sucrose and maltose with increase in the storage temperature and time. The concentration of glucose increased for 24 hrs of storage at all temperatures studied and then decreased with the storage time. Rhamnose in the extracts stored at 80$^{\circ}C$ for 72 hrs was identified and its concentration was increased at the higher storage temperature. The reduction of the concentrations of sugars related to the development of brown color during the processing and the storage.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.221-222
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• 2010

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.280-281
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• 2010

• Journal of Ginseng Research
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• 제6권2호
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• pp.143-148
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• 1982

This study was conducted to investigate to select the optimum condition for the sterilization of white ginseng powder. The powder heated at 100-121 $^{\circ}C$ for 1-30 minutes in a autoclave and irradiated at 2450 MHz microwave for 0.5-3.0 minutes was significantly changed in it's color density and become coagulated state, but their sterilizing effect on a general bacteria and 5. coli group were negligible. Meanwhile W- irradiation to the powder with thickness 1.5-10.5mm for 120 minutes was no changes in it's color. coagulated state and sterilizing effect, but 60Co-${\gamma}$ray irradiation at various dose with 0.1-2.0 Mrad was significant effect for sterilization and stability of the powder during the storage for 4 months at 25 $^{\circ}C$. The optimum dose of 60Co-${\gamma}$ ray irradiation was 0.5-0.7 Mrad.

• Journal of Ginseng Research
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• 제4권1호
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• pp.96-103
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• 1980

The effect of granule size of dried red ginseng extract on physical characteristics on tablet such as hardness, color, yield and disintegration time was studied. A moisture proofing agent was found to be necessary to form granules without serious absorption of moisture. The result showed that decrease in particle size resulted increase of values in weight, hardness and disintegration time. The granule size of 60∼80 mesh was found to be optimum for proper color uniformity and other physical characteristics of tables.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2008년도 춘계학술발표회
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• pp.276-277
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• 2008

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2008년도 춘계학술발표회
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• pp.156-157
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• 2008