• 제목/요약/키워드: Suillus

검색결과 35건 처리시간 0.023초

Molecular Taxonomical Re-classification of the Genus Suillus Micheli ex S. F. Gray in South Korea

  • Min, Young Ju;Park, Myung Soo;Fong, Jonathan J.;Seok, Soon Ja;Han, Sang-Kuk;Lim, Young Woon
    • Mycobiology
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    • 제42권3호
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    • pp.221-228
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    • 2014
  • The fungal genus Suillus Micheli ex S. F. Gray plays important roles in the survival and growth of plant seedlings. Humans have utilized these ectomycorrhizal fungi to enhance the nutrient uptake and defense systems of plants, particularly in the reforestation of coniferous forests. The genus Suillus is easily distinguishable by its distinctive morphological features, although the morphology of the fruiting body does not facilitate reliable interspecies discrimination. On the basis of micro-morphological features and internal transcribed spacer sequence analysis, we found that 51 of 117 Korean Suillus specimens had initially been misidentified. The list of the 12 Suillus species previously recorded in Korea was re-evaluated and revised to only eight distinct species: S. americanus, S. bovinus, S. granulatus, S. grevillei, S. luteus, S. pictus, S. placidus, and S. viscidus. We provide taxonomical descriptions for six of these species from the sample specimens.

젖비단 그물버섯(Suillus granulatus)으로부터 분리한 저분자 화합물 (Chemical Structures of Compounds Isolated from Mushroom Suillus granulatus)

  • 강희철;윤봉식;유승헌;유익동
    • 한국미생물·생명공학회지
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    • 제29권3호
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    • pp.149-154
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    • 2001
  • 버섯유래 저분자 대사산물을 탐색하던 중 대전근교에서 채집한 젖비단 그물버섯(Suillus granulatus)으로 부터 9종의 화합물을 분리하였다. 젖비단 그물버섯을 메탄올로 추출한 후 용매분획하여 그 중 대산산물이 많이 함유된 chloro-form 층 및 ethyl acetate층 에 대하여 각각 silica gel 및 Sephadx LH-20 column chromatography를 수행하였다. 주요대사산물을 함유하고 있는 분획물을 TLC및 분취용 HPLC를 사용하여 정제한 결과 9종의 화합물을 분리하였다. 분리된 화합물의 화학구조를 규명하기 위하여 NMR 및 mass 분석을 수행하여쓰며 그 결과 이들의 화합물은 4-hydro-xyphenylacetic acid, 4-hydroxybenzaldhyde, 2, 5-dihydro-xybenzoic acid methyl ester, 5-deoxy-5 methylthioadeno- sine. indole 3-carboxlic methyl ester indole-3-carbox-aldehyde 1,3,5-trihydroxy 7-methylanthraquinone nicotinamide, 3-geranylgeranyl-4-hydroxybenzoic acid로 동정하였다.

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Genet Variation of Ectomycorrhizal Suillus granulatus Fruiting Bodies in Pinus strobus Stands

  • Lee, Hwa-Yong;Koo, Chang-Duck
    • Mycobiology
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    • 제44권1호
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    • pp.7-13
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    • 2016
  • The genets of Suillus granulatus in a Pinus strobus stand ($13m{\times}60m$) were identified using random amplified polymorphic DNA molecular markers and the DNA of mushrooms that fruited for two years, and variations in genet size and distribution were analyzed. From a total of 116 mushrooms, 73 genets were identified and were grouped into three locations. The genets of mushrooms in close proximity differed from each other. The genet sizes varied at any of the three locations. The lengths of the identified genets in the pine stand ranged from 0.09 to 2.90 m. The average number of mushrooms per genet was 1.2 to 2.3, and the percentage of genets that were represented by a single mushroom was 44% to 94%. This variation in the genets of mushrooms in close proximity suggests that the ectomycorrhizal mycelial bodies of S. granulatus propagated sexually by fusing haploid spores derived from the mushrooms gills with below-ground mycelia. Therefore, it is necessary further to investigate the formation of new genets through spores in ectomycorrhizal fungal colonies.

Screening Test of Wild Mushroom Methanol Extracts for Fibrinolytic and $\alpha-Glucosidase$ Inhibitory Activity

  • 김준호
    • 대한의생명과학회지
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    • 제13권3호
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    • pp.245-249
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    • 2007
  • We investigated the fibrinolytic and ${\alpha}-glucosidase$ inhibitory activity of 55 wild mushroom methanol extracts. Among them, 14 mushrooms showed fibrinolytic activity. In particular, Amanita virgineoides showed the greatest enzyme activity (3.9 plasmin units/ml) by a fibrin plate assay. The fibrinolytic activities of Suillus pictus and Polypolellus varius were 3.8 plasmin units, and the activity of Gomphus fujisanensis was 2.8 plasmin units. Leccinum extremiorientale and Xerocomus nigromaculatus had the same activity with 2.3 plasmin units. In a ${\alpha}-glucosidase$ inhibitory activity test, Lactarius sp. showed the greatest inhibitory activity at 97.3%. The ${\alpha}-glucosidase$ inhibitory activities of Clitocybe odora, Xerocomus nigromaculatus, Melanoleuca melaleuca, Suillus pictus, and Gyroporus castaneus were 84.3%, 77.9%, 74.6%, 68.7%, and 65.4%, respectively. According to the results, because Suillus pictus and Xerocomus nigromaculatus have strong fibrinolytic and ${\alpha}-glucosidase$ inhibitory activities, the two mushrooms will be used as materials for the development of new biofunctional food.

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한국산(韓國産) 고등균류(高等菌類)(1) (Higher Fungi in Korea (1))

  • 석순자;김양섭;유영진;박동석
    • 한국균학회지
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    • 제23권2호통권73호
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    • pp.144-152
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    • 1995
  • 본 논문은 한국산 버섯류의 분포상과 자원탐색을 위한 연구조사중 주름버섯류내의 한국미기록 1속, 헛깔때기버섯속 Pseudoclitocybe과 미기록 5종, 헛깔때기버섯 Pseudoclitocybe cyathiformis(Bull.: Fr.) Sing., 한천비늘버섯 Pholiota brunnescense Smith & Hesler, 쥐방울먹물버섯 Coprinus angulatus Peck, 쌍포자외대버섯 Rhodophyllus bisporus Hongo, 끈적비단그물버섯 Suillus viscidipes Hongo과 복균류 1품종, 용문새주둥이버섯 Lysurus mokusin (L.; Pers.) Fr. f. sinnensis (Lloyd) Kobayasi 등이 판명되어 이를 보고한다. 색명은 "Methuen Handbook of Colour"(Kornerut & Wanscher, 1978)을 인용(引用)하였다. 채집(採集)된 모든 버섯표본(標本)은 농업과학기술원(農業科學技術院)에 보존(保存)되어 있다.

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순수배양 시 외생균근균의 질소원 요구성과 선호도 (Nitrogen Source-requirement and Preference of Ectomycorrhizal Fungi in Pure Culture)

  • 전성민;가강현
    • 한국균학회지
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    • 제41권3호
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    • pp.149-159
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    • 2013
  • 국내 산림에서 수집한 외생균근균(13속 42균주)을 질소원이 서로 다른 4종류의 시험배지에 접종하고 56일간 순수 배양하여 질소원 요구성과 선호도를 조사하였다. 그 결과, 일본연지그물버섯(KFRI 1224), 비단그물버섯(KFRI 1232), 끈적쓴맛그물버섯(KFRI 1383), 젖비단그물버섯(KFRI 1997), 알버섯류(KFRI 1434) 등이 질소원 요구성이 높은 것으로 조사되었다. 검은머리그물버섯(KFRI 1362)을 비롯하여 총 시험균주의 45%는 질산염보다 암모늄 형태의 무기질소원을 더 선호하였다. 반면 큰비단그물버섯(KFRI 1125)을 비롯한 총 시험균주의 36%는 질산염 형태의 무기질소원을 더 선호하였다.

Pisolthus tinctorius, Rhizopogon spp. 및 Suillus pictus균(菌)의 접종에 의한 리기테다소나무 묘목에 형성된 외생균근의 형태 (Morphology of Ectomycorrhizae of Pinus rigida × P. taeda Seedlings Inoculated with Pisolthus tinctorius, Rhizopogon spp. and Suillus pictus)

  • 이창헌;고대식
    • 한국산림과학회지
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    • 제82권4호
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    • pp.319-327
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    • 1993
  • 본(本) 연구(硏究)는 우리나라에 인공식재(人工植栽)된 몇몇 지역(地域)의 리기테다소나무림(林)에 자생(自生)하는 몇가지 균근균(菌根菌)을 분리(分離) 동정(同定)하고, 도입(導入) 균근균(菌根菌)인 Pt균(菌)과 분리된 자생(自生) 균근균(菌根菌) Ra균(菌), Rb균(菌), Sp균(菌) 등(等)의 접종에 의해 리기테다소나무묘(苗)에 형성(形成)된 균근(菌根)의 해부학적(解剖學的)인 형태(形態)를 조사(調査)하였던 바 다음과 같은 결과(結果)를 얻었다. 1. 분리(分離) 동정(同定)된 Rhizopogon rubescens, Rhizopogon sp., Suillus pictus균(菌)과 도입종(導入種)인 Pisolithus tinctorius균(菌) 등(等)을 리기테다소나무묘(苗)의 근부(根部)에 인공접종(人工接種)한 결과(結果) 균근(菌根)을 형성(形成)하였다. 2. 균근(菌根)이 형성(形成)된 뿌리는 균사(菌絲)가 뿌리 표면(表面)을 감싸고 있으며 Hartig net와 균투(菌套)가 발달(發達)하였다. 또한 Hartig net의 발달(發達)로 인(因)하여 세포간극(細胞間隙)이 두꺼워졌다. 3. 균근(菌根)의 세포에서는 비균근(非菌根)의 세포(細胞)에서보다 액포의 크기는 작았고, 세포질(細胞質)의 양(量)은 많았다. 그러나 핵(核)의 크기에는 큰 차이(差異)가 없었다.

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Variation in Sodium Chloride Resistance of Cenococcum geophilum and Suillus granulatus Isolates in Liquid Culture

  • Obase, Keisuke;Lee, Jong-Kyu;Lee, Sun-Keun;Lee, Sang-Yong;Chun, Kun-Woo
    • Mycobiology
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    • 제38권3호
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    • pp.225-228
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    • 2010
  • We studied the resistance of Cenococcum geophilum and Suillus granulatus isolates to NaCl during growth under axenic culture conditions. C. geophilum isolates displayed variations in NaCl resistance; mycelial growth of most isolates was inhibited above 200mM. All isolates of S. granulatus were tolerant to high NaCl content.

ITS 영역 증폭에 의한 소나무 송이균 뿌리 감염 확인 및 RAPD에 의한 타 균근과의 비교 (Identification of Tricholoma matsutake in a Pine Root by ITS Region Amplification and RAPD Analysis with Different Mycorrhiza)

  • 김명길;유선화;박원철;박현;가강현;손희경
    • Journal of the Korean Wood Science and Technology
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    • 제34권6호
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    • pp.96-103
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    • 2006
  • 대량 배양된 송이 균사를 배양병 안에 있는 소나무에 접종한 접종묘를 실시하였는데, 접종묘 내 소나무 뿌리내로 송이 균사가 침투하여 감염이 되었는지를 판별하는 방법으로 ITS (Internal transcribed spacer) 영역에서 특이 primer set인 ITS1-ITS4 (TCCGTAGGTGAACCTGCGG/TCCTCCGCTTATGATATGC)를 선택하여 정하고, 그를 바탕으로 홍천 시험지(강원도 홍천군 동면 노천리)와 홍릉수목원(서울시 동대문구 청량리 2동) 내에서 수집한 다른 균근과 비교하고자 RAPD (Rapid Amplified Polymorphic DNA) 기법으로 각 6종, 10 종의 균근을 동정하였다.

Iso-suillin Isolated from Suillus luteus, Induces G1 Phase Arrest and Apoptosis in Human Hepatoma SMMC-7721 Cells

  • Jia, Zhi-Qiang;Chen, Ying;Yan, Yong-Xin;Zhao, Jun-Xia
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권3호
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    • pp.1423-1428
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    • 2014
  • Iso-suillin, a natural product isolated from Suillus luteus, has been shown to inhibit the growth of some cancer cell lines. However, the molecular mechanisms of action of this compound are poorly understood. The purpose of this study was to investigate how iso-suillin inhibits proliferation and induces apoptosis in a human hepatoma cell line (SMMC-7721). We demonstrated the effects of iso-suillin on cell proliferation and apoptosis in SMMC-7721 cells, with no apparent toxicity in normal human lymphocytes, using colony formation assays and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis. Western blotting was used to examine the expression of $G_1$ phase-regulated and apoptosis-associated protein levels in iso-suillin treated SMMC-7721 cells. The results indicated that iso-suillin significantly decreased viability, induced $G_1$ phase arrest and triggered apoptosis in SMMC-7721cells. Taken together, these results suggest the potential of iso-suillin as a candidate for liver cancer treatment.