• Title/Summary/Keyword: Succinate

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A Study of the Effects of Na Ion on Codeposition of Particles in the Formation of Electroless Ni Composite Coatings (무전해 Ni 복합도금에서 분말의 공석에 미치는 Na 이온의 영향)

  • 이원해;이승평
    • Journal of the Korean institute of surface engineering
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    • v.22 no.2
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    • pp.101-108
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    • 1989
  • Effects of Na+ ion on zeta potential of SiC and Al2O3 particles suspended in nikel sufate and nickel chloride solutions were investigated. various complexing agents for Ni2+ ion were added to electroless Ni composite bath and the effects of the complexing agents on zeta potential and codeposition of the particles from the baths were studied. It was confirmed that Na+ ion was absorbed on the particles bringing about the positive surface charge and thus they promoted the entrapment of the particles into the nickel deposit. On the basis of these results it was possible to deposit SiCc particle in nickel chloride electrolyte containing complex agent such as trisodium citrate+sodium succinate.

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Effects of Salts on the Formation of $\alpha$-Calcium Sulfate Hemihydrated from by-Product Gypsum of Phosphoric Acid Process at Hydrothermal Condition (가압수열 수용액중에서 인산석고로부터 $\alpha$형 반수석고의 생성에 미치는 염류의 영향)

  • 이구종;최상흘
    • Journal of the Korean Ceramic Society
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    • v.24 no.4
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    • pp.343-348
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    • 1987
  • The effects of salts such as aluminum sulfate as inorganic salt(2-4%), and sodium salts of citrate, tartrate, succinate, potassium tartrate and gelatin as organic salts(0.1%) on the formation of ${\alpha}$-calcium sulfate hemihydrate from by-product gypsum of phosphoric acid process under hydrothermal condition at 123$^{\circ}C$ and 133$^{\circ}C$ were investigated. Aluminum sulfate solution exhibited the catalystic effected on the crystallization of ${\alpha}$-calcium sulfate hemihydrate of which was assumed in the prismatic form, and organic salts solution exhibited little effect on the catalystic action to the crystallization, than inorganic salts. In the acidic solution with sulfuric acid(pH=2), needle like crystal of calcium sulfate hemihydrate was obtained. Hydrothermal process with aluminum sulfate solution also showed certain amounts of impurity removal such as phosphorus penataoxide from calcium sulfate hemihydrate.

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PCA를 이용한 유전자 재조합 대장균의 ALA 생산공정의 해석

  • Gang, Tae-Hyeong;Jeong, Sang-Yun;Im, Yong-Sik;Kim, Chun-Gwang;Jeong, Sang-Uk;Lee, Jong-Il
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.157-160
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    • 2003
  • ALA is an intermediate in the tetrapyrrole biosynthesis pathway and has extensive applications as a biodegradable herbicide and insecticide as well as medical applications including photodynamic therapy of cancers. For the development of mass production process of ALA it is necessary to on-line monitor some metabolites such as glycine, succinate, LA and ALA. In this study, medium compositions and fermentation conditions were investigated for enhancement of ALA production by recombinant E. coli. A 2-dimensional fluorescence sensor was employed to monitor the bioprocess of ALA production. The monitored data is analyzed using principal component analysis, a powerful tool for multivariate statistical analysis.

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유전자 재조합 대장균을 이용한 5-Aminolevulinic acid(ALA)의 대량 생산 기술 및 모니터링 기술 개발

  • Jeong, Sang-Yun;Lee, Jong-Il;Scheper, Th.
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.87-90
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    • 2003
  • ALA is an essential intermediate in the tetrapyrrole biosynthesis pathway and has applications as a biodegradable herbicide and insecticide as well as medical applications including photodynamic therapy of cancers. For the development of mass production process of ALA it is necessary to on-line monitor some metabolites such as glycine, succinate, LA and ALA. In this work, medium compositions, pH conditions, induction and addition of LA were investigated to increase the production of ALA by recombinant E. coli. A 2-dimensional fluorescence sensor was used to monitor the processe and the fluorescence spectra were correlated with on- and off-line data like ALAS, ALAD, cell mass etc.

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Malonate Metabolism: Biochemistry, Molecular Biology, Physiology, and Industrial Application

  • Kim, Yu-Sam
    • BMB Reports
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    • v.35 no.5
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    • pp.443-451
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    • 2002
  • Malonate is a three-carbon dicarboxylic acid. It is well known as a competitive inhibitor of succinate dehydrogenase. It occurs naturally in biological systems, such as legumes and developing rat brains, which indicates that it may play an important role in symbiotic nitrogen metabolism and brain development. Recently, enzymes that are related to malonate metabolism were discovered and characterized. The genes that encode the enzymes were isolated, and the regulation of their expression was also studied. The mutant bacteria, in which the malonate-metabolizing gene was deleted, lost its primary function, symbiosis, between Rhizobium leguminosarium bv trifolii and clover. This suggests that malonate metabolism is essential in symbiotic nitrogen metabolism, at least in clover nodules. In addition to these, the genes matB and matC have been successfully used for generation of the industrial strain of Streptomyces for the production of antibiotics.

Conversion of Succinate-and Adipate-Coordinated Al(III) Complexes to AlN in $N_2$ and $NH_3$ Atmospheres (질소와 암모니아 분위기에서 알루미늄(III)의 호박산 및 아디프산 착물의 AlN으로의 변환)

  • 안상경;오창우;정우식
    • Journal of the Korean Ceramic Society
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    • v.33 no.4
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    • pp.455-463
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    • 1996
  • Aluminium nitride (AlN) powder was prepared by using aluminium (III) complexes with dibasic carboxylate ligands(adipato)(hydroxo) aluminium(III) and (hydroxo)(succinato)aluminium (III) as a precursor. The AlN pow-der was obtained by calcining the complexes without mixing any carbon source under a flow of ammonia at 120$0^{\circ}C$ Contary to the conventional carbothermal reduction and nitridiation the process of decarboniza-tion of the residual carbon was not required because of the reaction of ammonia with carbon at temperature >100$0^{\circ}C$. Fine AlN powder was also prepared by calcining a mixture of an (adipato)(hydroxo)aluminium(III) complex and carbon under a flow of nitrogen at 140$0^{\circ}C$ The AlN powders prepared were ultrafine and their morphology was almost the same as that of powders of two precursors.

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Factors affecting the protoplast formation and regeneration of Bacillus pumilus and Cellulomonas fimi for intergeneric protoplast fusion

  • Kim, D.M.;Lee, K.H.
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1986.12a
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    • pp.527.3-527
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    • 1986
  • Several factors predicted to affect the protoplast formation and regeneration were investigated. The optimum lysozyme, casamino acid and PVP concentration were 0.5 (mg/$m\ell$), 0.1 (%) and 1.5(%). In B. pumilus, Penicillin-G treatment concentration was 0.3 (U/$m\ell$) and optimum treatment period was transit log. phase. And in the case of Celm. fimi, 0.3 (U/$m\ell$) and initial log. phase. Osmotic stabilizer and di-cation for OSM medium of B.pumilus and Gelm .fimi were 25mM CaCl2, 0.5M sodium sucinate and 50mM MgCl$_2$, 100mM CaCl$_2$, 0.4M sodium succinate. The regeneration frequency of B.pumilus and Celm. fimi were 14.6(%) and 6.9(%).

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Mutational and Nutritional IMprovement of Tylosin Production (Tylosin 생성 우수 균주선별과 Tylosin 발효)

  • 이상희;정병철;이계준
    • Microbiology and Biotechnology Letters
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    • v.23 no.6
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    • pp.647-651
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    • 1995
  • A tylosin-hyperproductive mutant of S. fradiae MNU20 was isolated among 3500 strains obtaincd from either MNNG- or UV-treated Streptomyces fradiae NRRL2702. The composition of optimal medium for tylosin production was formulated as followed as: 4 g soluble starch, 1 g glucose, 1 g corn steep liquor, 7.5 ml soy bean oil, 0.2 g KH$_{2}$PO$_{4}$, 1 g Na$_{2}$S$_{2}$O$_{3}$$\cdot $5H$_{2}$O, 2 g CaCO$_{3}$, 2 g NaCl, 0.001 g CoCl$_{2}$$\cdot $6H$_{2}$O in 1 liter of distilled water. With the optimal medium, S. fradiae MNU20 was able to produce 159 mg tylosin (g biomass)$^{-1}$, indicating that tylosin productivity of Streptomyces fradiae NRRL2702 was increased 14 times higher by mutation. When the effect of valine, succinate, and natural zeolite on tylosin production was investigated by using the optimal medium, these substances essentially enhanced tylosin production and their addition time during culture period appeared to be critical for the increase of tylosin production.

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Bioconversion of fumaric acid to succinic acid using hollow fiber bioreactor (실관 생물반응기를 이용한 푸마르산으로부터 숙신산 생물전환)

  • Wi, Yeong-Jung;Yun, Jong-Seon;Min, Na-Yeong;Kim, Jin-Nam;Ryu, Hwa-Won
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.297-300
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    • 2000
  • Succinic acid was produced by Enterococcus faecalis RKY1 cells immobilized in hollow fiber bioreactor as an alternatively immobilized culture in bioconversion of fumaric acid to succinic acid. The feed was pumped through the shell side. As the flow rate of the feed was increased, the steady state was obtained more quickly. The steady state was reached after 24 hr cultivation in 0.25 ml/min, 12 hr in 0.5 ml/min, and 9 hr in 1.0 ml/min, respectively. The effect of medium pH on succinate production was also investigated. By changing the medium pH of 8.0, the succinic acid produced was increased about 16% than that of pH 7.0.

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Interfacial and Thermal Characteristics of Natural Fiber Composites Reinforced with Henequen Surface-Treated with EBI

  • Pang Yansong;Han Seong Ok;Cho Donghwan;Drzal Lawrence T.
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2004.10a
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    • pp.88-91
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    • 2004
  • In this study, a number of natural fiber henequen reinforced polymer matrix composites were successfully fabricated by means of a compression molding technique using chopped henequen fibers surface-treated with different electron beam irradiation (EBI) dosages, thermoplastic poly(butylene succinate), thermosetting unsaturated polyester and phenolic resins. Their interfacial and thermal characteristics were studied in terms of interfacial shear strength, fracture surface, dynamic mechanical properties, dimensional stability, and thermal stability using single fiber microbonding test, SEM, DMA, TMA, and TGA. The results show that their interfacial and thermal properties significantly depend on the intensity of EBl treatment on the natural fiber surface.

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