• Title/Summary/Keyword: Sub- resonance

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Improved Purification of Thermophilic FoF1-ATP Synthase c-Subunit Rings and Solid-State NMR Characterization of Them in Different Lipid Membranes

  • Bak, Suyeon;Kang, Su-Jin;Suzuki, Toshiharu;Yoshida, Masasuke;Fujiwara, Toshimichi;Akutsu, Hideo
    • Journal of the Korean Magnetic Resonance Society
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    • v.17 no.2
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    • pp.67-75
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    • 2013
  • ATP synthase produces ATP, a major energy source for metabolic processes in organisms, from ADP and inorganic phosphate in cellular membranes. ATP synthase is known as a rotary motor, in which the c-subunit ring functions as a rotor. In this work, we have tried to develop a more general preparation procedure of thermophilic $F_oc$-ring ($TF_oc$-ring) for NMR measurements. The expression of $TF_oF_1$ is easily affected by various experimental conditions such as temperature, shape and size of a flask, a volume of medium, and shaking rate of an incubator. Accordingly, we have tried to optimize the expression conditions of $TF_oF_1$. $TF_oc$-rings were purified from $TF_oF_1$ according to a reported method. We modified purification procedures to improve purity and yield of $TF_oc$. On top of them, we found a new combination of detergents for the purification at anion-exchange column chromatography. To examine the effect of lipid environments on the structure, the $TF_oc$-rings were reconstituted into two kinds of lipid bilayers, namely, saturated and unsaturated lipid ones. Then, we have compared characteristics of the $TF_oc$-ring structures in these membranes with solid-state NMR.

Magneto-optical Properties of 55Mn-doped SrTiO3 Single Crystal (55Mn이 첨가된 SrTiO3 단 결정의 광 전이 특성연구)

  • Bae, Kyu-Chan;Park, Jung-Il;Lee, Hyeong-Rag
    • Journal of the Korean Magnetics Society
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    • v.21 no.6
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    • pp.208-213
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    • 2011
  • We calculated the EPR (electron paramagnetic resonance) line-shape function. The line-widths of a -doped single crystal was studied as a function of the temperature with 0.5 and 2 at. at a frequency of (X-band). The line-width decreases with increasing temperature, such temperature behavior of the line-width can indicate a motional narrowing of the spectrum, when impurity ions substitute for host ions in an off-center position, and thus there can be fast jumping of dipoles between several symmetrically equivalent configurations. Therefore, the present technique is considered to be more convenient to explain the resonant system as in the case of other optical transition problems.

Synthesis of Monodisperse Iron-oxide Nanoparticles from Fe(acac)3 Precursor (Fe(acac)3 전구체를 사용한 균일한 산화철 나노입자 제조)

  • Kim, Dong Young
    • Journal of the Korean Magnetics Society
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    • v.24 no.1
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    • pp.18-21
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    • 2014
  • The microwave absorption ($P_{tot}$), which is the double integration value of ferromagnetic resonance signal, propositional to the saturation magnetization, and the increase of the $P_{tot}$ measured during the thermal reaction time expect the growth process of the nanoparticles. Therefore, in this work, we measured the $P_{tot}$ in order to obtain the growth time of iron oxide nanoparticles after thermal decomposition of $Fe(acac)_3$ precursor at aging temperature $T_a=273$, 300 and $324^{\circ}C$, respectively. The best condition for monodisperse nanoparticles was obtained at $T_a=300^{\circ}C$, which condition showed the most rapid increase of $P_{tot}$ with thermal reaction time. Finally, the rapid growth rate was necessary condition for the synthesis of iron-oxide monodisperse nanoparticles.

Evaluation of Treatment Plan Quality between Magnetic Resonance-Guided Radiotherapy and Volumetric Modulated Arc Therapy for Prostate Cancer

  • Chang Heon Choi;Jin Ho Kim;Jaeman Son;Jong Min Park;Jung-in Kim
    • Progress in Medical Physics
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    • v.33 no.4
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    • pp.121-128
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    • 2022
  • Purpose: This study evaluated the quality of plans based on magnetic resonance-guided radiotherapy (MRgRT) tri-Co-60, linac, and conventional linac-based volumetric modulated arc therapy (linac-VMAT) for prostate cancer. Methods: Twenty patients suffering from prostate cancer with intermediate risk who were treated by MAT were selected. Additional treatment plans (primary and boost plans) were generated based on MRgRT-tri-Co-60 and MRgRT-linac. The planning target volume (PTV) of MRgRT-based plans was created by adding a 3 mm margin from the clinical target volume (CTV) due to high soft-tissue contrast and real-time motion imaging. On the other hand, the PTV of conventional linac was generated based on a 1 cm margin from CTV. The targets of primary and boost plans were prostate plus seminal vesicle and prostate only, respectively. All plans were normalized to cover 95% of the target volume by 100% of the prescribed dose. Dosimetric characteristics were evaluated for each of the primary, boost, and sum plans. Results: For target coverage and conformity, the three plans showed similar results. In the sum plans, the average value of V65Gy of the rectum of MRgRT-linac (2.62%±2.21%) was smaller than those of MRgRT tri-Co-60 (9.04%±3.01%) and linac-VMAT (9.73%±7.14%) (P<0.001). In the case of bladder, the average value of V65Gy of MRgRT-linac was also smaller. Conclusions: In terms of organs at risk sparing, MRgRT-linac shows the best value while maintaining comparable target coverage among the three plans.

Studies on the influence factors of wind dynamic responses on hyperbolic cooling tower shells

  • ZHANG, Jun-Feng;LIU, Qing-Shuai;GE, Yao-Jun;ZHAO, Lin
    • Structural Engineering and Mechanics
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    • v.72 no.5
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    • pp.541-555
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    • 2019
  • Wind induced dynamic responses on hyperbolic cooling tower (HCT) shells are complicated functions of structure and wind properties, such as the fundamental frequency fmin, damping ratio ζ, wind velocity V, correlationship in meridian direction and so on, but comprehensions on the sensitivities of the dynamic responses to these four factors are still limited and disagree from each other. Following the dynamic calculation in time domain, features of dynamic effects were elaborated, focusing on the background and resonant components σB and σR, and their contributions to the total rms value σT. The σR is always less than σB when only the maximum σT along latitude is concerned and the contribution of σR to σT varies with responses and locations, but the σR couldn't be neglected for structural design. Then, parameters of the above four factors were artificially adjusted respectively and their influences on the gust responses were illustrated. The relationships of σR and the former three factors were expressed by fitted equations which shows certain differences from the existing equations. Moreover, a new strategy for wind tunnel tests aiming at surface pressures and the following dynamic calculations, which demands less experiment equipment, was proposed according to the influence from meridian correlationship.

Low Temperature Sintering and Microwave Dielectric Properties of Ca[Ti1-x(Ni1/3Nb2/3)x]O3 Ceramics (Ca[Ti1-x(Ni1/3Nb2/3)x]O3 세라믹스의 저온소결 및 마이크로파 유전특성)

  • Lee, Young-Gyu;Kim, Hyo-Tae;Nam, Joong-Hee;Kim, Jong-hee;Paik, Ungyu
    • Journal of the Korean Ceramic Society
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    • v.43 no.1 s.284
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    • pp.55-61
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    • 2006
  • The microwave dielectric properties and low temperature sintering of $Ca[Ti_{1-x}(Ni_{1/3}Nb_{2/3})_x]O_3$ system were investigated at the sintering temperature $1,200\~1,350^{\circ}C$. The density and quality factors $(Q{\times}f)$ increased while dielectric constants slightly decreased with the decrease of Ti. The dielectric constant, quality factor, and temperature coefficient of resonance frequency $(\tau_f)$ were 64, 17,000 GHz, and $-9.1\;ppm/^{\circ}C$ respectively, when $CaTi_{1/2}(Ni_{1/3}Nb_{2/3})_{1/2}O_3$ ceramics were sintered at $1,300^{\circ}C$ for 4 h. $2Li_2O-B_2O_3$ was added to $CaTi_{1/2}(Ni_{1/3}Nb_{2/3})_{1/2}O_3$ to decrease the sintering temperature for LTCC application. The microwave dielectric properties of the samples sintered at $925^{\circ}C$ for 2 h with the addition of $6\;wt\%\;2Li_2O-B_2O_3$ were $\varepsilon_r=48.7,\;Q{\times}f=8,460\;GHz$, and $\tau_f=+5.6ppm/^{\circ}C$. Compatibility test of the composition with silver electrode shows no reaction with silver electrode, implying the feasibility as a high-K LTCC material.