• Journal of the Korea Concrete Institute
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• v.16 no.5 s.83
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• pp.709-717
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• 2004

The existing AASHTO Standard Specification have some inadequacies in expressing wheel load distribution of bridge which has specific shape of curved bridge instead of straight bridge. Thus, this research presented the finite element analysis and modelling technique of prestressed concrete girder bridge having curved slab and the expression of wheel load distribution was suggested as the ratio of bending moment utilizing the result of finite element analysis of prestressed concrete girder bridge having cowed slab. The considered parameter of girder distribution expression is the curvature of slab, span length, girder space, cross beam space and number of lanes. Though the suggested girder distribution expression is generally underestimated below AASHTO Standard Specification, once the curvature of slab increases, the suggested expression gets larger than AASHTO LRFD Standard Specification.

• Journal of the Korean Magnetic Resonance Society
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• v.17 no.1
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• pp.30-39
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• 2013

Immortalization-upregulated protein-1 (IMUP-1) genes have been cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 gene is highly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. Previously, there were several problems for overexpression of IMUP-1 in bacterial expression systems including low solubility and aggregation due to unstructured property. To investigate the structural properties, it is necessary to obtain lots of pure and soluble proteins. Accordingly, the co-expression systems of bacterial chaperone proteins, GroEL-GroES, were used to increase solubility of IMUP-1. From the analysis of NMR and CD experiment data, it is suggested that the protein adopt typical the random coil properties in solution.

• Bulletin of the Korean Chemical Society
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• v.28 no.9
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• pp.1549-1552
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• 2007

The cytoplasmic domain of syndecan-4, a type I transmembrane heparan sulfate proteoglycan, was overexpressed as a fused form with the ubiquitin molecule in Escherichia coli, and the fusion protein was purified using immobilized metal affinity chromatography (IMAC). The cytoplasmic domain was released from its fusion partner by using yeast ubiquitin hydrolase (YUH), and subsequently purified by reverse phase chromatography. The integrity of the resulting peptide fragment was checked by MALDI-TOF and NMR spectroscopy. The yield of the peptide was 3.0-1.5 mg per liter in LB or minimal medium, respectively. The recombinant expression and purification of this domain will enable us its structural and functional studies using multidimensional NMR spectroscopy.

• Structural Engineering and Mechanics
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• v.31 no.2
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• pp.225-235
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• 2009

The present paper reviews the shear design (of reinforced concrete beam) provisions of four different national codes and proposes a new but simplified shear strength empirical expression, incorporating variables such as compressive strength of concrete, percentage of longitudinal and vertical steel/s, depth of beam in terms of shear span-to-depth ratio, for reinforced concrete (RC) beams without shear reinforcement. The expression is based on the experimental investigation on RC beams without shear reinforcement. Further, the comparisons of shear design provisions of four National codes viz.: (i) IS 456-2000, (iii) BS 8110-1997, (iv) ACI 318-2002 (v) EuroCode-2-2002 and the proposed expression for the prediction of shear capacity of normal beam/s, have been made by solving a numerical example. The results of the numerical example worked out suggest that there is need for revision in the shear design procedure of different codes. Also, the proposed expression is less conservative among the IS, BS & Eurocode.

• International Journal of High-Rise Buildings
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• v.5 no.4
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• pp.271-291
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• 2016

There is much architectural and engineering literature which discusses the virtues of exterior bracing and diagrid systems in regards to sustainability - two systems which generally reduce building materials, enhance structural performance, and decrease overall construction cost. By surveying past, present as well as possible future towers, this paper examines another attribute of these structural systems - the blend of structural functionality and aesthetics. Given the external nature of these structural systems, diagrids and exterior bracings can visually communicate the inherent structural logic of a building while also serving as a medium for artistic effect. Viewed in this light, the visual appeal of these systems can be enhanced to give a tower a more distinct urban identity. This entails the creation of structural elements that are aesthetically pleasing, geometrically coherent and that demonstrate dexterity of application in regards to a building's composition, while also respecting the laws of physics and mechanics. In this fashion, an artistic approach can exhibit structural systems as not just purely rational features that enable the construction of tall buildings, but as important visual components that afford opportunities for creative expression. This paper, therefore, synthesizes the concepts of structural performance and creative artistry to facilitate a better understanding of the aesthetic developments in skyscrapers worldwide.

• International Journal of High-Rise Buildings
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• v.11 no.1
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• pp.31-39
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• 2022

The seamless integration of the architecture and structure of a tall building plays a key role in establishing a recognizable and iconic design. The structural system developed for Shenzhen Rural Commercial Bank Headquarters (SRCBH) utilizes enhanced structural innovations unique to the tower's geometry to improve structural and sustainability performance. SRCBH utilizes a steel diagrid system pulled outside of the enclosure line with diaphragm forces resolved primarily by corner diagonal beams. During the design process the structural systems underwent performance based design and optimization for wind and seismic loading. Resiliency was prioritized for structural design as well as fire resistance. More closely integrating the structure of a building with its architecture and sustainability goals can lead to unique and innovative towers with a timeless expression.

• Journal of Korean Society of Forest Science
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• v.84 no.1
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• pp.77-86
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• 1995

To effectively modify tree function with genetic engineering, transgenes must be expressed at the proper level in the appropriate tissues at suitable developmental stages. Toward understanding the spatial and temporal expression of transgenes in woody plants, transgene expression was evaluated in three greenhouse-grown, transgenic lines of Populus alba ${\times}$ P. grandidentata hybrid clone 'Hansen'. All transgenic poplar lines possess constructs containing the bacterial nopaline synthase(nos) promoter linked to a neomycin phosphotransferase II(NPT II) selectable marker gene. In addition, each transgenic poplar line contains one of the following gene constructs : 1) a wound-inducible potato proteinase inhibitor II (pin2) promoter linked to a chloramphenicol acetyltransferase(CAT) reporter gene. 2) a nos promoter linked to a PIN2 structural gene : or 3) a Cauliflower Mosaic Virus 35s promoter linked to a PIN2 structural gene. Polymerase chain reaction(PCR) was used to verify the presence of foreign genes in the poplar genome. Enzyme-linked immunosorbent assays(ELISAs) were used to evaluate organ specific expression of the nos-NPT II construct. NPT II expression was detected in leaves, petioles, stems, and roots of transgenic poplar, thereby indicating that the nos promoter is potentially effective for general constitutive expression of transgenes. NPT expression varied among transgenic poplar lines and among organs for one transgenic line, Tr15. With Tr15, NPT II levels were highest in older leaves and petioles. These results indicate that screening of several transgenic lines may be required to identify lines with optimal transgene expression.

• Journal of KSNVE
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• v.4 no.4
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• pp.487-496
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• 1994

Recently, the application of viscoelastic material in the field of vibration isolation has gradually increased due to its achievement in structural damping capacity, and many of the theoretical and experimental study has been carried out. In this study, the dynamic characteristics of the visoelastically supported cantilever beam, of which govering equation is based on the Bernoulli- Euler equation, is analyzed theoretically and experimentally. Expression for stiffness of multi-layered viscoelastic materal has been developed using variables such as frequency and number of layers, and further, based on this expression, damping characteristic of the beam is investigated with experimental verification.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.154.1-154.1
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• 2003

TCTP is presented to have a retinol binding protein (RBP)-like structure by domain search. Human cellular RBP (CRBP) plays a key role in the intercellular transfer of retinol. Modulation of its expression is known to contribute to tumor growth and progression via retinoid-mediated signaling. Changes in the expression of TCTP have also been reported to be associated with carcinogenesis. Therefore, the attempt to establish the interactive relationship between the human TCTP and CRBP with retinol will be helpful in further understanding the cell signaling of TCTP. (omitted)

• The KIPS Transactions:PartD
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• v.14D no.6
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• pp.605-614
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• 2007

As a standard query language to search XML data, XQuery and XPath were proposed by W3C. By widely using XQuery and XPath languages, recent researches focus on the development of query processing algorithm and data structure for efficiently processing XML query with the enormous XML database system. Recently, when processing XML path expressions, the concept of the structural join which may determine the structural relationship between XML elements, e.g., ancestor-descendant or parent-child, has been one of the dominant XPath processing mechanisms. However, structural joins which frequently occur in XPath query processing require high cost. In this paper, we propose a new structural join algorithm, called SISJ, based on our structured index, called SI, in order to process XPath queries efficiently. Experimental results show that our algorithm performs marginally better than previous ones. However, in the case of high recursive documents, it performed more than 30% by the pruning feature of the proposed method.