[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.6564/JKMRS.2013.17.1.030

Expression and Purification of Unstructured Protein, IMUP-1, using Chaperone Co-expression System for NMR Study

Yi, Jong-Jae (College of pharmacy, CHA University)
Yoo, Jung Ki (College of pharmacy, CHA University)
Kim, Jin Kyeoung (College of pharmacy, CHA University)
Son, Woo Sung (College of pharmacy, CHA University)

Publication Information

Journal of the Korean Magnetic Resonance Society / v.17, no.1, 2013 , pp. 30-39 More about this Journal

Abstract

Immortalization-upregulated protein-1 (IMUP-1) genes have been cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 gene is highly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. Previously, there were several problems for overexpression of IMUP-1 in bacterial expression systems including low solubility and aggregation due to unstructured property. To investigate the structural properties, it is necessary to obtain lots of pure and soluble proteins. Accordingly, the co-expression systems of bacterial chaperone proteins, GroEL-GroES, were used to increase solubility of IMUP-1. From the analysis of NMR and CD experiment data, it is suggested that the protein adopt typical the random coil properties in solution.

Keywords

IMUP-1; Chaperone; Purification; CD; NMR;

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