• Bulletin of the Korean Chemical Society
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• 제34권4호
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• pp.1232-1236
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• 2013

The Cu cation binding sites of $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex have been investigated to explain the $[Cu{\cdot}DNA]$ biological activity caused by the Cu association to DNA. The structure of $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex was investigated by electrospray ionization mass spectrometry (ESI-MS). The fragmentation patterns of $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex were analyzed by MS/MS spectra. In the MS/MS spectra of $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex, three fragment ions were observed with the loss of d(CpG), {d(CpG) + Cyt}, and {d(CpG) + Cyt + dR}. The Cu cation binds to d(CpG) mainly by substituting the $H^+$ of phosphate group. Simultaneously, the Cu cation prefers to bind to a guanine base rather than a cytosine base. Five possible geometries were considered in the attempt to optimize the $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex structure. The ab initio calculations were performed at B3LYP/6-31G(d) level.

• 한국건설관리학회:학술대회논문집
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• 한국건설관리학회 2008년도 정기학술발표대회 논문집
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• pp.85-92
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• 2008

리모델링 공사는 신축공사와 달리 철거공사가 주공정으로 선행되어야 하는데 현행 철거공사는 개략적 수준의 철거관리계획에 의한 생산성만을 강조한 철거 형태로 인해 폐자재 재활용이 되지 않고 있어 자원낭비, 환경파괴를 가중시키고 있으며, 철거작업 중 작업자의 안전과 구조체의 안전등이 전혀 고려되지 않고 철거하는 것이 현실이다. 따라서 친환경 철거공사가 되기 위해서는 프로세스의 개선이 우선 되어야하며 그 단계를 1) 철거관련 조사분석, 2) 철거관리 도서작성, 3) 철거관리 시공계획서작성, 4) 철거시공 및 감리, 5) 철거 후 관리의 5단계로 구분하였으며, 특히 철거도면 작성으로 표준철거 WBS가 구축되고, 철거장비 및 공법, 폐기물 투하, 철거공정계획 수립이 가능하며 마감재 철거 후 구조체의 안전점검을 실시하므로써 철거중 불가피한 보수보강이 가능하며, 철거완료 Check list 작성에 따라 리모델링 본 공사의 Database 활용이 가능하게 되었다. 본 논문에서는 현행 리모델링 철거공사 프로세스(AS-IS)의 문제점을 분석하고 개선된 친환경 리모델링 철거공사 프로세스(TO-BE)를 IDEF0 모델을 사용하여 제안하고자 한다.

• 한국수산과학회지
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• 제44권6호
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• pp.597-604
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• 2011

Using 108 strains of Vibrio parahaemolyticus isolated from seawater, we investigated ampicillin-resistance profiles and the genetic characterization of ${\beta}$-lactamase (VPA0477). All of the strains studied, except one strain, were resistant to ampicillin. However, the strain that was susceptible to ampicillin had the same ${\beta}$-lactamase gene as the ampicillin-resistant strains. We compared ${\beta}$-lactamase promoter region sequences among five strains, including both ampicillin-resistant and -susceptible strains. In the susceptible strain, a nucleotide at position -19 in the methionine initiation codon for ${\beta}$-lactamase was not present in the ampicillin-resistant strains. The genes in the region containing the gene VPA0477 were present in all of the tested strains, and LA-PCR analysis showed that the distance between VPA0474 and VPA0479 in all of the V. parahaemolyticus samples was precisely 5.7 kb. In V. parahaemolyticus ${\beta}$-lactamase, four important structural features that are conserved in Class A ${\beta}$-lactamases were present in the deduced amino acid sequences. Taken together, our study demonstrates that V. parahaemolyticus ${\beta}$-lactamase is included in the Class A ${\beta}$-lactamase group, and some nucleotides within the promoter region are of particular importance for ${\beta}$-lactamase activity.

• 산업경영시스템학회지
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• 제40권3호
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• pp.27-37
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• 2017

As the global uncertainty of manufacturing has increased and the quality problem has become global, the recall has become a fatal risk that determines the durability of the company. In addition, as the convergence of PSS (product-service system) product becomes common due to the development of IT convergence technology, if the function of any part of hardware or software does not operate normally, there will be a problem in the entire function of PSS product. In order to manage the quality of such PSS products in a stable manner, a new approaches is needed to analyze and manage the hardware and software parts at the same time. However, the Fishbone diagram, FTA, and FMEA, which are widely used to interpret the current quality problem, are not suitable for analyzing the quality problem by considering the hardware and software at the same time. In this paper, a quality risk assessment model combining FTA and FMEA based on defect rate to be assessed daily on site to manage quality and fishbone diagram used in group activity to solve defective problem. The proposed FTA-FMEA based risk assessment model considers the system structure characteristics of the defect factors in terms of the relationship between hardware and software, and further recognizes and manages them as risk. In order to evaluate the proposed model, we applied the functions of ITS (intelligent transportation system). It is expected that the proposed model will be more effective in assessing quality risks of PSS products because it evaluates the structural characteristics of products and causes of defects considering hardware and software together.

• Archives of Pharmacal Research
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• 제28권2호
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• pp.177-179
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• 2005

Nine known diarylheptanoids (1-9) isolated from the barks of Alnus japonica were evaluated for their inhibitory activities on nitric oxide (NO) and prostagrandin $E_2$ (COX-2) production in interferon-${\gamma}$ (INF-${\gamma}$) and lipopolysaccharide (LPS)-activated RAW 264.7 cells in vitro. The NO and COX-2 levels were moderately reduced by the addition of compounds (1-9). Among these compounds, compounds 6 and 8 inhibited NO production in a dose dependent manner with an $IC_{50}$ of 16.7 and $27.2\;{\mu}g/mL$, respectively (positive control, L-NMMA; $22.8\;{\mu}g/mL$), and compounds 6, 7, 8, and 9 reduced the COX-2 level in a dose dependent manner with an $IC_{50}$ of 20.7, 25.7, 25.0, and $27.3\;{\mu}g/mL$, respectively (positive control, indomethacin; $26.2\;{\mu}g/mL$). An analysis of the structural activity relationship among these diarylheptanoids suggests that the presence of a keto-enol group in the heptane moiety or a caffeoyl group in the aromatic ring were important for the efficacy on the inhibitory activities of NO and COX-2 production.

• Archives of Pharmacal Research
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• 제29권1호
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• pp.50-58
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• 2006

Translationally controlled tumor protein (TCTP), also known as histamine releasing factor (HRF), is found abundantly in different eukaryotic cell types. The sequence homology of TCTP between different species is very high, belonging to the MSS4/DSS4 superfamily of proteins. TCTP is involved in both cell growth and human late allergy reaction, as well as having a calcium binding property; however, its primary biological functions remain to be clearly elucidated. In regard to many possible functions, the TCTP of Plasmodium falciparum (Pf) is known to bind with an antimalarial agent, artemisinin, which is activated by heme. It is assumed that the endoperoxide-bridge of artemisinin is opened up by heme to form a free radical, which then eventually alkylates, probably to the Cys14 of PfTCTP. Study of the docking of artemisinin with heme, and subsequently with PfTCTP, was carried out to verify the above hypothesis on the basis of structural interactions. The three dimensional (3D) structure of PfTCTP was built by homology modeling, using the NMR structure of the TCTP of Schizosaccharomyces pombe as a template. The quality of the model was examined based on its secondary structure and biological function, as well as with the use of structure evaluating programs. The interactions between artemisinin, heme and PfTCTP were then studied using the docking program, FlexiDock. The center of the peroxide bond of artemisinin and the Fe of heme were docked within a short distance of $2.6{\AA}$, implying the strong possibility of an interaction between the two molecules, as proposed. When the activated form of artemisinin was docked on the PfTCTP, the C4-radical of the drug faced towards the sulfur of Cys14 within a distance of $2.48{\AA}$, again suggesting the possibility of alkylation having occurred. These results confirm the proposed mechanism of the antimalarial effect of artemisinin, which will provide a reliable method for establishing the mechanism of its biological activity using a molecular modeling study.

• 건축역사연구
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• 제14권4호
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• pp.117-135
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• 2005

This paper is a study of the possibility of experience and expression in the architecture of Louis I. Kahn by focusing on the characters of entrance, court and window/wall of his public buildings. In the course of composition, Kahn defined the entrance, court and window/wall as an connecting elements and elements of boundary. The characters revealed by these elements or rooms give the clue to insight Kahn's thought of relation of interior and exterior space or inner and outer space. Following are the characters of these elements. First, a entrance reveals the fact that inner space separates from outer space by connecting these two space and giving the value to inner space as the entity and totality like outer space. The entrance gives its ontological being to human subjects not by vision but experience and expression which is the essence of commonness, that is, Silence. Kahn made the possibility of activity amplify in this common and silent space. Second, this entrance is connected with wide and huge central space not individual spaces of interior space. This extreme procedure of entering makes human subject feel sublime of intoner space. And the central spaces becomes another exterior or another world in the inner world of architecture by the lights from above and by having the boundary wall which shows same pattern of exterior wall. Third, Kahn regarded a window as the giver of lights not as the medium of vision connecting inner space with outer. He tried to connect interior with exterior through the being and character of the light expressed in the interior. And in his buildings, interior space is connected with exterior by expressing the purpose of building, composition of inner space, structural truth and construction facts through the Form, a pattern of wall, details and ornamental joints. By practicing this thoughts in the real buildings, Kahn tried to gave aura to both the interior space and entity of architecture which is regarded as micro universe like flowers, rocks and human beings.

• Journal of the Korean Data and Information Science Society
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• 제26권6호
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• pp.1377-1386
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• 2015

한 나라 전체의 경제활동 수준을 나타내는 경기의 변동과 밀접한 관계를 지닌 지표로서 GDP갭을 꼽을 수 있다. GDP갭은 초과수요압력이나 고용사정에 대한 정보를 제공하기 때문에 중앙은행의 통화정책 수행시 중요한 고려변수로 꼽히고 있다. 그러나, GDP갭 총량만으로는 최근의 경제구조 변화라든지 대내외 경제여건의 영향 등을 살펴볼 수 없는 등 제한적인 부분이 있다. 본 논문에서는 통계적 필터링 기법에 의해 새로운 갭을 추정하고 다양한 물가영향 모형을 설정하여 각 요인들이 인플레이션에 미치는 영향력을 추정하는 한편 동 요인들의 영향력이 시간에 따라 변화하는지도 분석하였다. 분석결과, GDP갭의 물가영향력이 2000년대 들어 대체로 그 영향력이 축소되는 것으로 추정된 반면, 글로벌갭이 국내 물가에 미치는 영향력은 증대된 것으로 나타났다. 이러한 변화는 최근의 저물가 현상이 국내요인과 더불어 세계 경기침체에서 비롯된 수출의 둔화와 같은 국외여건에 영향을 받았다는 것을 의미한다.

• 한국주거학회논문집
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• 제22권2호
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• pp.53-61
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• 2011

This study aims to examine the characteristics of the changes in residents' perception of neighborhood and sense of neighborhood according to the residential structural changes caused by city renewal projects such as old urban large redevelopment. Research findings benefits of the residential environment changes after the development are environment improvement, convenient traffic, leisure activity increase, and green area increase, whereas their drawbacks are encroachment of sunshine and view, However, with the regard to the relationship with neighbors, paired-sample t-test results showed there were no statistically significant differences between before and after the development. It appeared to be that the community residential complex development did not influence the relationship with neighbors. ANOVA was used to examine the differences in the neighborhood perception among age categories. The results showed that there were no differences between age groups but when there were changes in their life cycle, residents showed differences in their neighborhood perception among age groups. The neighborhood perception was measured on 5-point Likert Scale in four factors of intimacy, participation, reliability, and group identity. The perception decreased somewhat after the development and the group identity, especially, showed a significant difference (p<.001). This was considered to be caused by a sense of incongruity due to the influx of different social-economic groups.

• Bulletin of the Korean Chemical Society
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• 제32권10호
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• pp.3675-3681
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• 2011

Fusarium oxysporum, an important pathogen that mainly causes vascular or fusarium wilt disease which leads to economic loss. Disruption of gene encoding a heterotrimeric G-protein-${\beta}$-subunit (FGB1), led to decreased intracellular cAMP levels, reduced pathogenicity, colony morphology, and germination. The plant defense protein, Nicotiana alata defensin (NaD1) displays potent antifungal activity against a variety of agronomically important filamentous fungi. In this paper, we performed a molecular modeling and docking studies to find vital amino acids which can interact with various antifungal compounds using Discovery Studio v2.5 and GRAMMX, respectively. The docking results from FGB1-NaD1 and FGB1-antifungal complexes, revealed the vital amino acids such as His64, Trp65, Ser194, Leu195, Gln237, Phe238, Val324 and Asn326, and suggested that the anidulafungin is a the good antifungal compound.The predicted interaction can greatly assist in understanding structural insights for studying the pathogen and host-component interactions.