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http://dx.doi.org/10.5657/KFAS.2011.0597

Genetic Characterization of β-lactamase (VPA0477) in Vibrio parahaemolyticus  

Lee, Nam-Hyung (Department of Food Science and Biotechnology, Kunsan National University)
Song, Hyun-Jung (Department of Food Science and Biotechnology, Kunsan National University)
Park, Chang-Soo (Department of Food Science and Technology, Catholic University of Daegu)
Kim, Hee-Dai (Department of Biotechnology and Biomedicine, Chungbuk Provincial College)
Park, Kwon-Sam (Department of Food Science and Biotechnology, Kunsan National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.44, no.6, 2011 , pp. 597-604 More about this Journal
Abstract
Using 108 strains of Vibrio parahaemolyticus isolated from seawater, we investigated ampicillin-resistance profiles and the genetic characterization of ${\beta}$-lactamase (VPA0477). All of the strains studied, except one strain, were resistant to ampicillin. However, the strain that was susceptible to ampicillin had the same ${\beta}$-lactamase gene as the ampicillin-resistant strains. We compared ${\beta}$-lactamase promoter region sequences among five strains, including both ampicillin-resistant and -susceptible strains. In the susceptible strain, a nucleotide at position -19 in the methionine initiation codon for ${\beta}$-lactamase was not present in the ampicillin-resistant strains. The genes in the region containing the gene VPA0477 were present in all of the tested strains, and LA-PCR analysis showed that the distance between VPA0474 and VPA0479 in all of the V. parahaemolyticus samples was precisely 5.7 kb. In V. parahaemolyticus ${\beta}$-lactamase, four important structural features that are conserved in Class A ${\beta}$-lactamases were present in the deduced amino acid sequences. Taken together, our study demonstrates that V. parahaemolyticus ${\beta}$-lactamase is included in the Class A ${\beta}$-lactamase group, and some nucleotides within the promoter region are of particular importance for ${\beta}$-lactamase activity.
Keywords
Vibrio parahaemolyticus; ${\beta}$-lactamase; VPA0477 gene; Ampicillin;
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1 Son KT, Oh EG, Lee TS, Lee HJ, Kim PH and Kim JH. 2005. Antimicrobial susceptibility of Vibrio parahaemolyticus and Vibrio alginolyticus from farms on the southern coast of Korea. J Kor Fish Soc 38, 365-371.
2 Teo JWP, Suwanto A and Poh CL. 2000. Novel $\ss$-lactamase genes from two environmental isolates of Vibrio harveyi. Antimicrob Agents Chemother 44, 1309-1314.   DOI   ScienceOn
3 Tomasz A and Waks S. 1975. Mechanism of action of penicillin: triggering of the pneumococcal autolytic enzyme by inhibitors of cell wall synthesis. Proc Natl Acad Sci USA 72, 4162-4166.   DOI   ScienceOn
4 Yu HS, Park KB, Oh EG, Lee TS, Shin SB, Kwon JY, Kim JH and Son KT. 2010. Trimethoprim resistance by class I integron in Vibrio parahaemolyticus from a fish farm. Kor J Fish Aquat Sci 43, 125-130.
5 Weng SF, Chao YF and Lin JW. 2004. Identification and characteristic analysis of the ampC gene encoding $\ss$-lactamase from Vibrio fischeri. Biochem Biophys Res Commun 314, 838-843.   DOI   ScienceOn
6 National Committee for Clinical Laboratory Standards. 2002. Performance standards for antimicrobial susceptibility testing. Twelfth informational supplement M100-S12. Wayne, PA:19087-19098. U.S.A.
7 Lee H, Oh YH, Park SG and Choi SM. 2007. Antibiotic susceptibility and distribution of Vibrio parahaemolyticus isolated from the seafood. Kor J Env Hlth 33, 16-20.
8 Lee HW, Lim SK and Kim MN. 2009. Characteristics of ampicillin- resistant Vibrio spp. isolated from a west coastal area of Korea peninsula. J Kor Fish Soc 42, 20-25.
9 Lee KW and Park KS. 2010. Antibiotic-resistance profiles and the identification of the ampicillin-resistance gene of Vibrio parahaemolyticus isolated from seawater. Kor J Fish Aquat Sci 43, 637-641.
10 Makino K, Oshima K, Kurokawa K et al., 2003. Genome sequence of Vibrio parahaemolyticus : a pathogenic mechanism distinct from that of V. cholerae . Lancet 361, 743-749.   DOI   ScienceOn
11 Massova I and Mobashery S. 1998. Kinship and diversification of bacterial penicillin-binding proteins and $\ss$-lactamases. Antimicrob agents Chemother 42, 1-17.   DOI   ScienceOn
12 NVRQS (National Veterinary Research and Quarantine Service). Monitoring of microbial resistance on the food animals and meats. 1-92.
13 Oh EG, Yu HS, Shin SB, Son KT, Park KB, Kwon JY, Lee TS and Lee HJ. 2008. Trimethoprim resistance of Vibrio parahaemolyticus isolated from the fish farm. J Kor Fish Soc 41, 324-329.
14 Park KS, Ono T, Rokuda M, Jang MH, Okada K, Iida T and Honda T. 2004. Functional characterization of two type III secretion systems of Vibrio parahaemolyticus. Infect Immun 72, 6659-6665.   DOI   ScienceOn
15 Highton CE and Hobbs DG. 1971. Penicillin and cell wall synthesis: a study of Bacillus licheniformis by electron microscopy. J Bacteriol 106, 646-658.
16 Sakazaki R, Tamura K, Kato T, Obara Y and Yamai S. 1968. Studies on the enteropathogenic, facultatively halophilic bacterium, Vibrio parahaemolyticus . 3. Enteropathogenicity. Jpn J Med Sci Biol 21, 325-331.   DOI
17 Son JC, Park SW and Min KJ. 2003. Environmental and antimicrobial characteristics of Vibrio spp. isolated from fish, shellfish and brackish water samples in Gyeongbuk eastern coast. Kor J Env Hlth 29, 94-102.
18 Choury D, Aubert G, Szajnert MF, Azibi K, Delpech M and Paul G. 1999. Characterization and nucleotide sequence of CARB-6, a new carbenicillin-hydrolyzing $\ss$-lactamase from Vibrio cholerae. Antimicrob Agents Chemother 43, 297-301.
19 Honda T and Iida T. 1993. The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct haemolysin and related haemolysins. Rev Med Microbiol 4, 106-113.   DOI   ScienceOn
20 Kaneko T and Colwell RR. 1975. Adsorption of Vibrio parahaemolyticus onto chitin and copepods. Appl Microbiol 29, 269-274.   DOI
21 Kodama T, Hiyoshi H, Gotoh K, Akeda Y, Matsuda S, Park KS, Cantarelli VV, Iida T and Honda T. 2008. Identification of two translocon proteins of Vibrio parahaemolyticus type III secretion system 2. Infect Immun 76, 4282-4289.   DOI   ScienceOn
22 Lee CY, Cheng MF, Yu MS and Pan MJ. 2002. Purification and characterization of a putative virulence factor, serine protease, from Vibrio parahaemolyticus . FEMS Microbiol Lett 19, 31-37.
23 Kodama T, Rokuda M, Park KS, Cantarelli VV, Matsuda S, Iida T and Honda T. 2007. Identification and characterization of VopT, a novel ADP-ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type III secretion system 2. Cell Microbiol 9, 2598-2609.   DOI   ScienceOn
24 Lambert HP and O'Grady FW. 1992. Antibiotics and chemotherapy. In Veterinary medicine, 6th ed., Churchill Livingstone. New York. U.S.A., 130-139.