Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic Characterization of β-lactamase (VPA0477) in Vibrio parahaemolyticus

장염비브리오가 보유하는 β-lactamase (VPA0477)의 유전학적 특성

  • Lee, Nam-Hyung (Department of Food Science and Biotechnology, Kunsan National University) ;
  • Song, Hyun-Jung (Department of Food Science and Biotechnology, Kunsan National University) ;
  • Park, Chang-Soo (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Kim, Hee-Dai (Department of Biotechnology and Biomedicine, Chungbuk Provincial College) ;
  • Park, Kwon-Sam (Department of Food Science and Biotechnology, Kunsan National University)
  • 이남형 (군산대학교 식품생명공학과) ;
  • 송현정 (군산대학교 식품생명공학과) ;
  • 박창수 (대구카톨릭대학교 식품생명공학과) ;
  • 김희대 (충북도립대학 바이오생명의약과) ;
  • 박권삼 (군산대학교 식품생명공학과)
  • Received : 2011.09.28
  • Accepted : 2011.10.27
  • Published : 2011.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Using 108 strains of Vibrio parahaemolyticus isolated from seawater, we investigated ampicillin-resistance profiles and the genetic characterization of ${\beta}$-lactamase (VPA0477). All of the strains studied, except one strain, were resistant to ampicillin. However, the strain that was susceptible to ampicillin had the same ${\beta}$-lactamase gene as the ampicillin-resistant strains. We compared ${\beta}$-lactamase promoter region sequences among five strains, including both ampicillin-resistant and -susceptible strains. In the susceptible strain, a nucleotide at position -19 in the methionine initiation codon for ${\beta}$-lactamase was not present in the ampicillin-resistant strains. The genes in the region containing the gene VPA0477 were present in all of the tested strains, and LA-PCR analysis showed that the distance between VPA0474 and VPA0479 in all of the V. parahaemolyticus samples was precisely 5.7 kb. In V. parahaemolyticus ${\beta}$-lactamase, four important structural features that are conserved in Class A ${\beta}$-lactamases were present in the deduced amino acid sequences. Taken together, our study demonstrates that V. parahaemolyticus ${\beta}$-lactamase is included in the Class A ${\beta}$-lactamase group, and some nucleotides within the promoter region are of particular importance for ${\beta}$-lactamase activity.

Keywords

References

  1. Choury D, Aubert G, Szajnert MF, Azibi K, Delpech M and Paul G. 1999. Characterization and nucleotide sequence of CARB-6, a new carbenicillin-hydrolyzing $\ss$-lactamase from Vibrio cholerae. Antimicrob Agents Chemother 43, 297-301.
  2. Highton CE and Hobbs DG. 1971. Penicillin and cell wall synthesis: a study of Bacillus licheniformis by electron microscopy. J Bacteriol 106, 646-658.
  3. Honda T and Iida T. 1993. The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct haemolysin and related haemolysins. Rev Med Microbiol 4, 106-113. https://doi.org/10.1097/00013542-199304000-00006
  4. Kaneko T and Colwell RR. 1975. Adsorption of Vibrio parahaemolyticus onto chitin and copepods. Appl Microbiol 29, 269-274. https://doi.org/10.1046/j.1365-2672.1999.00622.x
  5. Kodama T, Hiyoshi H, Gotoh K, Akeda Y, Matsuda S, Park KS, Cantarelli VV, Iida T and Honda T. 2008. Identification of two translocon proteins of Vibrio parahaemolyticus type III secretion system 2. Infect Immun 76, 4282-4289. https://doi.org/10.1128/IAI.01738-07
  6. Kodama T, Rokuda M, Park KS, Cantarelli VV, Matsuda S, Iida T and Honda T. 2007. Identification and characterization of VopT, a novel ADP-ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type III secretion system 2. Cell Microbiol 9, 2598-2609. https://doi.org/10.1111/j.1462-5822.2007.00980.x
  7. Lambert HP and O'Grady FW. 1992. Antibiotics and chemotherapy. In Veterinary medicine, 6th ed., Churchill Livingstone. New York. U.S.A., 130-139.
  8. Lee CY, Cheng MF, Yu MS and Pan MJ. 2002. Purification and characterization of a putative virulence factor, serine protease, from Vibrio parahaemolyticus . FEMS Microbiol Lett 19, 31-37.
  9. Lee H, Oh YH, Park SG and Choi SM. 2007. Antibiotic susceptibility and distribution of Vibrio parahaemolyticus isolated from the seafood. Kor J Env Hlth 33, 16-20.
  10. Lee HW, Lim SK and Kim MN. 2009. Characteristics of ampicillin- resistant Vibrio spp. isolated from a west coastal area of Korea peninsula. J Kor Fish Soc 42, 20-25.
  11. Lee KW and Park KS. 2010. Antibiotic-resistance profiles and the identification of the ampicillin-resistance gene of Vibrio parahaemolyticus isolated from seawater. Kor J Fish Aquat Sci 43, 637-641.
  12. Makino K, Oshima K, Kurokawa K et al., 2003. Genome sequence of Vibrio parahaemolyticus : a pathogenic mechanism distinct from that of V. cholerae . Lancet 361, 743-749. https://doi.org/10.1016/S0140-6736(03)12659-1
  13. Massova I and Mobashery S. 1998. Kinship and diversification of bacterial penicillin-binding proteins and $\ss$-lactamases. Antimicrob agents Chemother 42, 1-17. https://doi.org/10.1093/jac/42.1.1
  14. National Committee for Clinical Laboratory Standards. 2002. Performance standards for antimicrobial susceptibility testing. Twelfth informational supplement M100-S12. Wayne, PA:19087-19098. U.S.A.
  15. NVRQS (National Veterinary Research and Quarantine Service). Monitoring of microbial resistance on the food animals and meats. 1-92.
  16. Oh EG, Yu HS, Shin SB, Son KT, Park KB, Kwon JY, Lee TS and Lee HJ. 2008. Trimethoprim resistance of Vibrio parahaemolyticus isolated from the fish farm. J Kor Fish Soc 41, 324-329.
  17. Park KS, Ono T, Rokuda M, Jang MH, Okada K, Iida T and Honda T. 2004. Functional characterization of two type III secretion systems of Vibrio parahaemolyticus. Infect Immun 72, 6659-6665. https://doi.org/10.1128/IAI.72.11.6659-6665.2004
  18. Sakazaki R, Tamura K, Kato T, Obara Y and Yamai S. 1968. Studies on the enteropathogenic, facultatively halophilic bacterium, Vibrio parahaemolyticus . 3. Enteropathogenicity. Jpn J Med Sci Biol 21, 325-331. https://doi.org/10.7883/yoken1952.21.325
  19. Son JC, Park SW and Min KJ. 2003. Environmental and antimicrobial characteristics of Vibrio spp. isolated from fish, shellfish and brackish water samples in Gyeongbuk eastern coast. Kor J Env Hlth 29, 94-102.
  20. Son KT, Oh EG, Lee TS, Lee HJ, Kim PH and Kim JH. 2005. Antimicrobial susceptibility of Vibrio parahaemolyticus and Vibrio alginolyticus from farms on the southern coast of Korea. J Kor Fish Soc 38, 365-371.
  21. Teo JWP, Suwanto A and Poh CL. 2000. Novel $\ss$-lactamase genes from two environmental isolates of Vibrio harveyi. Antimicrob Agents Chemother 44, 1309-1314. https://doi.org/10.1128/AAC.44.5.1309-1314.2000
  22. Tomasz A and Waks S. 1975. Mechanism of action of penicillin: triggering of the pneumococcal autolytic enzyme by inhibitors of cell wall synthesis. Proc Natl Acad Sci USA 72, 4162-4166. https://doi.org/10.1073/pnas.72.10.4162
  23. Yu HS, Park KB, Oh EG, Lee TS, Shin SB, Kwon JY, Kim JH and Son KT. 2010. Trimethoprim resistance by class I integron in Vibrio parahaemolyticus from a fish farm. Kor J Fish Aquat Sci 43, 125-130.
  24. Weng SF, Chao YF and Lin JW. 2004. Identification and characteristic analysis of the ampC gene encoding $\ss$-lactamase from Vibrio fischeri. Biochem Biophys Res Commun 314, 838-843. https://doi.org/10.1016/j.bbrc.2003.12.171

Cited by

  1. Antimicrobial-resistance Profiles and Virulence Genes of Vibrio parahaemolyticus Isolated from Seawater in the Wando Area vol.47, pp.3, 2014, https://doi.org/10.5657/KFAS.2014.0220
  2. Application of the β-lactamase (VPA0477) Gene for the Detection of Vibrio parahaemolyticus by Polymerase Chain Reaction vol.47, pp.6, 2014, https://doi.org/10.5657/KFAS.2014.0740