• KSBB Journal
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• v.9 no.2
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• pp.165-173
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• 1994

In this paper we have described the structural characterization of recombinant bovine somatotropin produced in Escherichia coli. Recombinant bovine somatotropin consists of 191 amino acid residues with a calculated molecular weight of 21,802 Da. For fragmentation of recombinant bovine somatotropin, we have used trypsin, Staphylococcus aureus V8 pretease, CNBr, and mild acid hydrolysis method. Digestion and cleavage with these proteases and chemicals yielded peptides of various size for amino acid sequence determination. The N-terminal sequence analysis was carried out up to thirty residues. Because the design of the recombinant bovine somatotropin gene for expression was such that the coding sequence begins with an initiation codon, AUG, before Ala, the first amino acid of bovine somatotropin, we could expect the initial amino acid as N-formyl Met. But the first amino acid of this protein, expressed in E. coli cells as inclusion bodies, was Ala. And the amino acid composition of RP-HPLC purified recombinant bovine somatotropin was determined and no essencial difference was observed. The amino acid sequence of the recombinant bovine somatotropin was identical to that predicted from its recombinant gene. There was no processing or replacement of amino acid residues in recombinant bovine somatotropin expressed in E. coli. The hydropathy plot of recombinant bovine somatotropin revealed a hydrophobic region at the NH2-terminus and hydrophilic region at the COOH-terminus. The E. coli expression system is thought to be valuable for the expression of recombinant bovine somatotropin because protein was processed to remove the N-terminal Met residue by methionyl-aminopeptidase autonomously.

• Computational Structural Engineering
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• v.10 no.4
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• pp.315-325
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• 1997

This paper describes the application of discretized continuum-type optimality criteria (DCOC) and the development of optimum design program for the multispan partially prestressed concrete beams. The cost of construction as objective function which includes the costs of concrete, prestressing steel, non-prestressing steel and formwork is minimized. The design constraints include limits on the maximum deflection, flexural and shear strengths, in addition to ductility requirements, and upper and lower bounds on design variables as stipulated by the design Code. Based on Kuhn-Tucker necessary conditions, the optimality criteria are explicitly derived in terms of the design variables-effective depth, eccentricity of prestressing steel and non-prestressing steel ratio. The prestressing profile is prescribed by parabolic functions. The self-weight of the structure is included in the equilibrium equation of the real system, as is the secondary effect resulting from the prestressing force. An iterative procedure and computer program for updating the design variables are developed. Two numerical examples of multispan PPC beams with rectangular cross-section are solved to show the applicability and efficiency of the DCOC-based technique.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.19 no.1 s.71
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• pp.47-54
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• 2006

In this study, an optimum support design problem is considered to minimize displacement of stacked plates under self weight condition. During the displacement analysis, several kinds of contact arise between the plates themselves and support bar. These can be easily considered if commercial analysis software, which provides capability to solve the contact problem, is used. It is found, however, that the computing time is extraordinarily long due possibly to the generality of the software and also to the ignorance of the control parameters used in the software. In this paper, the contact condition is imposed directly by the authors, while the software is used only to solve the ordinary displacement analysis problem. In this way, the computing time is decreased remarkably by more than 30 times, while yielding the same accurate results. Optimization is conducted based on this efficient analysis method to find minimum number of supporting bars using the response surface algorithm.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.22 no.4
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• pp.335-341
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• 2009

In this paper, review of developed MLS-based finite elements and a proposal for their applications are described. The shape functions and their derivatives of MLS-based finite elements are constructed using Moving-Least Square approximation. In MLS-based finite element, using the adequate influence domain of weight function used in MLS approximation, kronecker delta condition could be satisfied at the element boundary. Moreover, because of the characteristics of MLS approximation, we could easily add extra nodes at an arbitrary position in MLS-based finite elements. For these reasons, until now, several variable-node elements(2D variable element for linear case and quadratic case and 3D variable-node elements) and finite crack elements are developed using MLS-based finite elements concept. MLS-based finite elements could be extended to 2D variable-node triangle element, 2D finite crack triangle element, variable-node shell element, finite crack shell element, and 3D polyhedron element. In this paper, we showed the feasibility of 3D polyhedron element at the case of femur meshing.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.21 no.5
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• pp.421-427
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• 2008

A robot crane truck is developed by the Bridge Inspection Robot Development Interface(BRIDI) for an automated and/or teleoperated bridge inspection. This crane truck looks similar to the conventional bucket crane, but is much smaller in size and light-weight. At the end of the telescoping boom which is 12m long, a robot platform is mounted which allows the operator to scan the bridge structure under the deck trough the camera. Boom vibration induced by wind and deck movement can cause serious problems in this scanning system. This paper presents a control system to mitigate such vibration of the robot boom. In the proposed control system, an actuator is installed at the end of the working boom. This control system is studied using a mathematical model analysis with LQ control algorithm and a scaled model test in the laboratory. The study indicates that the proposed system is efficient for the vibration control of the robot booms, thereby demonstrating its immediate applicability in the field.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.29 no.6
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• pp.539-545
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• 2016

UPS system in the liquefied natural gas(LNG) receiving terminal is one of the fundamental equipment that need to sustain operation during earthquake. In this study, modal identification test of UPS system was performed based on IEEE Std. 693-2005 and natural frequencies and modal damping, mode shapes had been identified. In addition, tri-axial time history test was performed to check the behavior and stress of the equipment during earthquake. Eigenvalue analysis was performed and analysis model was modified by reflecting the results of the test. Static analysis by dead weight and response spectrum analysis were performed to compare the combined stresses with the stress results of test. Dynamic characteristics and combined stresses under seismic load condition of the improved analysis model were similar to the test results and in this regard the compatibility was proved.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.30 no.4
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• pp.289-296
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• 2017

In this paper, geometrical optimization for newly designed flapping mechanism for insect-like micro air vehicle is presented. The mechanism uses strings to convert rotation of motor to reciprocating wing motion to reduce the total weight and inertial force. The governing algorithm of movement of the mechanism is established considering the characteristic of string that only tensile force can be acted by string, to optimize the kinematics. Modified pattern search method which is complemented to avoid converging into local optimum is adopted to the geometrical optimization of the mechanism. Then, prototype of the optimized geometry is produced and experimented to check the feasibility of the mechanism and the optimization method. The results from optimization and experiment shows good agreement in flapping amplitude and other wing kinematics. Further research will be conducted on dynamic analysis of the mechanism and detailed specification of the prototype.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.30 no.6
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• pp.479-484
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• 2017

In this paper, finite element analysis of Steel-Concrete panel(SCP) was conducted considering the local buckling behavior and the optimized design of shear studs arrangement was studied by comparing with design guidelines. If the spacing of the studs of SCP is widened, it is easy to be manufactured and the weight fo members become lighter. On the other hand, the steel plate would be vulnerable to the local buckling behavior. Therefore, the guidance and design of SCP limit the maximum spacing of the studs to prevent the development of shear cracks and local buckling, however this is based on the design criteria of the other composite structures. Parameter studies with changes in stud spacing on steel plate and SCP are conducted and the obtained result was compared with values given in design guidelines.

• Steel and Composite Structures
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• v.22 no.3
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• pp.537-565
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• 2016

Australian railway networks possess a large amount of aging timber components and need to replace them in excess of 280 thousands $m^3$ per year. The relatively high turnover of timber sleepers (crossties in a plain track), bearers (skeleton ties in a turnout), and transoms (bridge cross beams) is responsible for producing greenhouse gas emissions 6 times greater than an equivalent reinforced concrete counterparts. This paper presents an innovative solution for the replacement of aging timber transoms installed on existing railway bridges along with the incorporation of a continuous walkway platform, which is proven to provide environmental, safety and financial benefits. Recent developments for alternative composite materials to replace timber components in railway infrastructure construction and maintenance demonstrate some compatibility issues with track stiffness as well as structural and geometrical track systems. Structural concrete are generally used for new railway bridges where the comparatively thicker and heavier fixed slab track systems can be accommodated. This study firstly demonstrates a novel and resilient alterative by incorporating steel-concrete composite slab theory and combines the capabilities of being precast and modulated, in order to reduce the depth, weight and required installation time relative to conventional concrete direct-fixation track slab systems. Clear benefits of the new steel-concrete composites are the maintainability and constructability, especially for existing railway bridges (or brown fields). Critical considerations in the design and finite element modelling for performance benchmarking of composite structures and their failure modes are highlighted in this paper, altogether with risks, compatibilities and compliances.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2002.04a
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• pp.123-130
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• 2002

A general formulation for shape design sensitivity analysis over three dimensional beam structure is developed based on a variational formulation of the beam in linear elasticity. Sensitivity formula is derived based on variational equations in cartesian coordinates using the material derivative concept and adjoint variable method for the displacement and Von-Mises stress functionals. Shape variation is considered for the beam shape in general 3-dimensional direction as well as for the orientation angle of the beam cross section. In the sensitivity expression, the end points evaluation at each beam segment is added to the integral formula, which are summed over the entire structure. The sensitivity formula can be evaluated with generality and ease even by employing piecewise linear design velocity field despite the bending model is fourth order differential equation. For the numerical implementation, commercial software ANSYS is used as analysis tool for the primal and adjoint analysis. Once the design variable set is defined using ANSYS language, shape and orientation variation vector at each node is generated by making finite difference to the shape with respect to each design parameter, and is used for the computation of sensitivity formula. Several numerical examples are taken to show the advantage of the method, in which the accuracy of the sensitivity is evaluated. The results are found excellent even by employing a simple linear function for the design velocity evaluation. Shape optimization is carried out for the geometric design of an archgrid and tilted bridge, which is to minimize maximum stress over the structure while maintaining constant weight. In conclusion, the proposed formulation is a useful and easy tool in finding optimum shape in a variety of the spatial frame structures.