• Animal Bioscience
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• 제34권7호
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• pp.1210-1220
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• 2021

Objective: Unlike mammals, goose fatty liver shows a strong tolerance to fatty acids without obvious injury. Stearyl-coenzyme A desaturase 1 (SCD1) serves crucial role in desaturation of saturated fatty acids (SAFs), but its role in the SAFs tolerance of goose hepatocytes has not been reported. This study was conducted to explore the role of SCD1 in regulating palmitic acid (PA) tolerance of goose primary hepatocytes. Methods: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide was examined to reflect the effect of PA on hepatocytes viability, and quantitative polymerase chain reaction was used to detect the mRNA levels of several genes related to endoplasmic reticulum (ER) stress, inflammation, and apoptosis, and the role of SCD1 in PA tolerance of goose hepatocytes was explored using RNA interfere. Results: Our results indicated that goose hepatocytes exhibited a higher tolerant capacity to PA than human hepatic cell line (LO2 cells). In goose primary hepatocytes, the mRNA levels of fatty acid desaturation-related genes (SCD1 and fatty acid desaturase 2) and fatty acid elongate enzyme-related gene (elongase of very long chain fatty acids 6) were significantly upregulated with 0.6 mM PA treatment. However, in LO2 cells, expression of ER stress-related genes (x box-binding protein, binding immunoglobulin protein, and activating transcription factor 6), inflammatory response-related genes (interleukin-6 [IL-6], interleukin-1β [IL-1β], and interferon-γ) and apoptosis-related genes (bcl-2-associated X protein, b-cell lymphoma 2, Caspase-3, and Caspase-9) was significantly enhanced with 0.6 mM PA treatment. Additionally, small interfering RNA (siRNA) mediated downregulation of SCD1 significantly reduced the PA tolerance of goose primary hepatocytes under the treatment of 0.6 mM PA; meanwhile, the mRNA levels of inflammatory-related genes (IL-6 and IL-1β) and several key genes involved in the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT), forkhead box O1 (FoxO1), mammalian target of rapamycin and AMPK pathways (AKT1, AKT2, FoxO1, and sirtuin 1), as well as the protein expression of cytochrome C and the apoptosis rate were upregulated. Conclusion: In conclusion, our data suggested that SCD1 was involved in enhancing the PA tolerance of goose primary hepatocytes by regulating inflammation- and apoptosis-related genes expression.

• 한국식품영양학회지
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• 제26권3호
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• pp.553-564
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• 2013

The core ingredient of traditional Korean style soy sauce is soy bean without any wheat or rice incorporated. National brands as well as regional micro-brewed companies constitute the soy sauce market in Korea. The present study investigated the physico-chemical and sensory properties of soy sauces produced by small-scale or mass-production. Additionally, the key physico-chemical parameters sufficiently representing the critical sensory characteristics have been identified. Ten types of soy sauce brewed by the Korean traditional method were selected for the study. Among these samples, seven types were brewed in small-scales in the Gyeonggi-do region whereas the other 3 types were mass-production products of major national brands. The total solid, reducing sugar, salinity, sugar content, amino nitrogen, CIELAB, acidity, and pH of soy sauce samples were measured for the physico-chemical analysis. A generic descriptive analysis was conducted to analyze the sensory characteristics of the samples using six trained panelists. The descriptive panel developed 21 sensory attributes. The data were statistically analyzed using ANOVA, PCA and PLSR. Overall, the micro-brewed products showed significantly higher value of salinity and acidity but lower content of reducing sugar than the mass-production products. The micro-brewed soy sauces elicited stronger fermented flavor, sourness, and bitterness whereas the national brand products elicited stronger alcoholic odor, sweetness and umami taste. Sugar content, acidity, and amino nitrogen showed strong relationships with fish sauce flavor, umami taste, and rich flavor. Salinity was closely related to the overall flavor intensity.

• 한국환경교육학회지:환경교육
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• 제5권1호
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• pp.19-33
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• 1993

This result showed that the middle school students' response for the environment condition was highly negative. The major findings of this study are as follows. 1. Students would become to know the environmental pollution through reading of newspapers and TV rather than school education. 2. Students' response for air and water pollution was seriously accepted in city areas than in country areas, and in large cities than in small cities. 3. Students' response for air pollution showed that the quality of air was getting worse and major factor of air pollution was the exhausts of automobiles. 4. It showed that students' concern for water pollution was increased and water pollution was being accelerated by the increase of domestic and industrial sewages, the overuse of the agricultural chemicals, the entrophication and acid rain. 5. Students thought that soil pollution was mainly due to factory sewage, life sewage, heavy metal and agricultural medicines and so on. But now they think it is due to the degenerated and inseparable things such as used vinyle for farm and plastics. 6. Most students thought of the pollution of our country as serious. But they thought it could be removed if we tried to get rid of pollution. 7. Now students' consciousness to protect the nature took an active interest turn and was strong. Putting these various findings together, I suggested that, the efforts to turn students affirmative consciousness for the environment and a powerful plan by the nation to take off pollution should be needed. Also the education to enforce the environment preservation had to be needed.

• 한국미생물·생명공학회지
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• 제39권3호
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• pp.200-209
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• 2011

동물은 고기, 우유, 및 달걀과 같은 중요한 산물을 생산한다. 현대사회의 도시화과정 및 고도 성장은 보다 많은 동물성 산물을 요구하고 작은 농장에서 밀집화된 사육이 이루어지게 되는 원인이 되었다. 결과적으로 가축 분뇨생산량이 증가되었으며, 그에 따른 악취문제도 필연적인 결과로 나타났다. 분뇨로 인한 악취문제를 해결하지 못할 경우 축산업의 지속적인 발전과 환경개선을 이루는 큰 장애가 될 것이다. 축산환경으로 생산되는 악취는 휘발성지방산, 암모니아와 휘발성아민, 인돌과 페놀, 그리고 황화합물 네 종류로 분류된다. 질소성분의 절반이상은 뇨로 배설되기 때문에 동물을 사육하는 과정에서 저단백 사료와 합성아미노산을 급여시키고, 소화효소나 미생물제제 혹은 생균제를 사료에 첨가시켜줄 경우 질소원의 배출량을 감소시켜줄 수 있는 중요한 방법이 될 것이다. 따라서, 축산환경의 개선과 축산분야의 생산성을 향상시켜주기 위하여 사료 및 보조사료를 급여하는 다양한 시도가 진행되고 있다.

• 농촌지도와개발
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• 제21권4호
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• pp.1093-1124
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• 2014

농학계열 대학의 역할에 대한 논의들은 주로 농업의 본원적 기능인 농산물생산과 사회적 필요에 의해 새롭게 중요성이 강조되는 기능들로 구분되는데, 녹색혁명과 농업인 노령화가 심한 상황에서 농학계 학문은 현상유지 정도만이 필요한 분야로 바라보는 시각이 강하다. 그러나 농업은 전통적인 농산물 생산중심에서 벗어나고, 그 개념과 내용이 확장되면서, 농학적 배경지식이 필요한 분야는 보다 세밀해지고 확대되고 있다. 하지만 대학평가의 가늠자로 평가받는 취업률 조사에서 농학계열 대학의 역할이 반영된 분야는 영농인 육성으로 보는 시각이 강하다. 영농업종사자를 제외한 농산업부문은 농학계열 학문분야와 다른 모든 학문분야가 한데 묶인 공통의 일반산업부문 범주로 구성되어, 농산업부문에 취업을 해도 전공과 관련 없는 분야에 취업한 것으로 볼 수 있는 문제가 있다. 이러한 배경에서 본 연구는 농학계열 대학 졸업생의 농산업분야 취업상황을 중심으로 인력육성측면에서 농학계열 대학의 갖는 의의를 살펴보았다. 농학계열 학문연구 분야는 국가과학기술표준분류 중분류 16개 분야로 구분하고, 취업분야는 산업연관부문 통합소분류 168개를 활용하여 전국 37개 대학 220개 학과를 대상으로 2013년 졸업생의 취업상황을 조사하였다. 조사결과 전체 취업자 3명 중 1명인 33.0%가 농산업분야에 진출하고 있었고, 이 중 3.6%가 농산업분야에 창업하고 있었다. 특히 영농종사자의 경우 2013년 국내 대학졸업자의 0.1%만이 취업하는 것으로 조사되었으나, 조사결과는 13.3%로 농업인 육성에 농학계열 대학의 기여가 큼을 확인할 수 있었다. 연구결과를 통해 볼 때, 취업률 조사방식의 문제로 제대로 역할을 평가받지 못하고 있는 것으로 보이나, 농학계열 대학의 농업, 농산업분야에 대한 기여를 확인할 수 있었다.