• Journal of Korean Clinical Nursing Research
• /
• v.22 no.3
• /
• pp.352-358
• /
• 2016

Purpose: This study was a methodological research implemented to establish basic data for controlling the job rotation stress of nurses and effectively managing human resources by reflecting the Nurse Job Rotation Stress Scale (NJRS) developed by Huang and others into the domestic nursing environment and verifying the use of measurement tools. Methods: The study selected nurses working at four hospitals, with over 300 beds, in Seoul, Gyeongi-do, and Busan, as its research subjects and verified the content validity, construct validity, concurrent validity, and internal consistency reliability of the measurement tool. Results: The results ultimately formed eight questions from three sub-factors (3 emotional response questions, 2 daily life questions, and 3 communication questions). In terms of reliability, emotional response issued a Cronbach's of .78, daily life issued a Cronbach's of .75, and communication issued a Cronbach's of .86. Conclusion: This study is expected to be used as basic data for achieving effective human resource management by calculating the stress of clinical nurses resulting from job rotation, but the researcher believes that the measurement tools must be expanded by confirming additional job rotation stress questions which reflect the organizational culture of Korea.

• Journal of Microbiology
• /
• v.37 no.3
• /
• pp.141-147
• /
• 1999

Thirteen transcriptionally-fused carbon starvation mutants, derived from Pseudomonas putida ATCC 12633, were analyzed for their survivability and transcriptional induction profiles upon carbon starvation. One of these mutants, MK114, which exhibited the lowest survivability and the highest induction rate, was selected and further examined under different starvation (nitrogen and phosphate) and stress (osmolarity, H2O2, salts, alcohol, and heat) conditions. Under all tested conditions MK114 induced ${\beta}$-galactosidase activity, implying that the interrupted gene (cst114) is a general starvation and stress response gene. The rate of induction ranged from 2.6-fold for phosphate starvation to 3.7-fold for osmotic shock. The mini-Tn5 flanking DNA was cloned from the chromosome of MK114. The cloned DNA fragment exhibited carbon starvation activity, indicating that this fragment contains a carbon starvation-related promoter region. This region was partially sequenced. Possible physiological roles of Cst114 in a carbon sensing mechanism and in other stress responses are also discussed.

• Journal of the Korean Society of Physical Medicine
• /
• v.13 no.3
• /
• pp.99-111
• /
• 2018

PURPOSE: This study was conducted to identify the effects of sympathetic conversation on stress, anxiety and depression, and muscle tone in chronic stroke patients. METHODS: Patients were randomly assigned to either an experimental group (EG, n=7) or control group (CG, n=7). Both groups participated in a pretest before intervention. Subjects were asked to undergo: 1) electroencephalogram, 2) Stress Response Inventory, 3) Hospital Anxiety and Depression Scale, 4) muscle tone and stiffness testing. After the pretest, EG received sympathetic conversation and CG received a simple explanation about stroke recovery and rehabilitation. Following the intervention, both groups were immediately administered a post test. RESULTS: In EG, the electroencephalogram relative alpha power was significantly increased (p<.05), while the electroencephalogram relative gamma power was significantly decreased (p<.05). The Stress Response Inventory and Hospital Anxiety and Depression Scale scores decreased significantly in both groups (p<.05). In addition, muscle tone and stiffness decreased significantly in the EG (p<.05) CONCLUSION: The results of the present study indicate that sympathetic conversation had a positive effect on stress, anxiety and depression, and muscle tone in patients with chronic stroke. Therefore, sympathetic conversation could be used to improve not only psychological problems in chronic stroke patients including stress and anxiety, but also physical conditions including muscle tone.

• Journal of Applied Biological Chemistry
• /
• v.48 no.4
• /
• pp.178-182
• /
• 2005

Nitric oxide (NO) produced from endothelial cells plays a critical role in vascular physiology. The regulation of endothelial NO synthase (eNOS) involves various mechanisms including multiple Ser/Thr phosphorylations. Recently, eNOS-Ser617 was newly recognized to be phosphorylated in response to humoral factors including vascular endothelial growth factor. However, it remains unknown whether and how eNOS-Ser617 phosphorylation is stimulated by shear stress, the primary stimulus of endothelial NO production. This issue was explored in the present study using cultured bovine aortic endothelial cells (BAECs). Over-expression of a constitutively active protein kinase B(Akt) mutant in BAECs increased Ser617 phosphorylation while constitutively active protein kinase A mutant had no effect. When BAECs were subjected to an arterial level of laminar shear stress, eNOS-Ser617 phosphorylation was clearly increased in a time-dependent manner. Shear stress also stimulated Akt phosphorylation at Thr308, one of the key regulatory sites. The time courses of eNOS-Ser617 and Akt-Thr308 phosphorylations appeared to be very similar. These results suggested that eNOS-Ser617 phosphorylation, mediated by Akt, is a physiological response to the mechanical shear stress, involved in the regulation of NO production in endothelial cells.

• Journal of Microbiology and Biotechnology
• /
• v.21 no.10
• /
• pp.1064-1068
• /
• 2011

The osmotolerant yeast, Candida magnoliae, which was isolated from honeycomb, produces erythritol from sugars such as fructose, glucose, and sucrose. Erythrose reductase in C. magnoliae (CmER) reduces erythrose to erythritol with concomitant oxidation of NAD(P)H. Sequence analysis of the 5'-flanking region of the CmER gene indicated that one putative stress response element (STRE, 5'-AGGGG-3'), found in Saccharomyces cerevisiae, exists 72 nucleotides upstream of the translation initiation codon. An enzyme activity assay and semiquantitative reverse transcription polymerase chain reaction revealed that the expression of CmER is upregulated under osmotic and salt stress conditions caused by a high concentration of sugar, KCl, and NaCl. However, CmER was not affected by osmotic and oxidative stress induced by sorbitol and $H_2O_2$, respectively. The basal transcript level of CmER in the presence of sucrose was higher than that in cells treated with fructose and glucose, indicating that the response of CmER to sugar stress is different from that of GRE3 in S. cerevisiae, which expresses aldose reductase in a sugarindependent manner. It was concluded that regulation of CmER differs from that of other aldose reductases in S. cerevisiae.

• Journal of the Korean Wood Science and Technology
• /
• v.34 no.5
• /
• pp.19-28
• /
• 2006

There has been considerable research in recent times in light-timber med structures in fires. These structures have included horizontal (floor-like) panels in bending and walls under eccentric and approximately concentric vertical loading. It has been shown that compression properties are the most dominant mechanical properties in affecting structural response of these structures in fire. Compression properties have been obtained by various means as functions of one variable only, temperature. It has always been expected that compression properties would be significantly affected by moisture and stress, as well. However, these variables have been largely ignored to simplify the complex problem of predicting the response of light-timber framed structures in fire. Full-scale experiments on both the panels and walls have demonstrated the high level of significance of moisture and stress for a limited range of conditions. Described in this paper is an overview of these conditions and experiments undertaken to obtain compression properties as a functions of moisture, stress and temperature. The experiments limited temperatures to $20{\sim}100^{\circ}C$. At higher temperatures moisture vaporizes and moisture and stress are less significant. Described also is a creep model for wood at high temperatures.

• Computational Structural Engineering
• /
• v.10 no.3
• /
• pp.185-193
• /
• 1997

The order of the stress singularity that occurs at the termination of an interface between materials exhibiting bilinear stress-strain response under plane strain conditions has been calculated, The governing equation of elasticity together with traction-free boundary condition and interface continuity condition defines a two-point boundary value problem. The stress components near the free edge are assumed to be proportional to r/sup s-1/, with solutions existing only for certain values of s. Finding these values entails the solution of an eigenvalue problem. Because it has been impossible to integrate the differential equations analytically, the integration has been performed numerically with a shooting method coupled with a Newton improvement scheme.

• Mycobiology
• /
• v.37 no.3
• /
• pp.161-170
• /
• 2009

Cryptococcus neoformans is a basidiomycete human fungal pathogen that causes meningoencephalitis in both immunocompromised and immunocompetent individuals. The ability to sense and respond to diverse extracellular signals is essential for the pathogen to infect and cause disease in the host. Four major stress-activated signaling (SAS) pathways have been characterized in C. neoformans, including the HOG (high osmolarity glycerol response), PKC/Mpk1 MAPK (mitogen-activated protein kinase), calcium-dependent calcineurin, and RAS signaling pathways. The HOG pathway in C. neoformans not only controls responses to diverse environmental stresses, including osmotic shock, UV irradiation, oxidative stress, heavy metal stress, antifungal drugs, toxic metabolites, and high temperature, but also regulates ergosterol biosynthesis. The PKC(protein kinase C)/Mpk1 pathway in C. neoformans is involved in a variety of stress responses, including osmotic, oxidative, and nitrosative stresses and breaches of cell wall integrity. The $Ca^{2+}$/calmodulin- and Ras-signaling pathways also play critical roles in adaptation to certain environmental stresses, such as high temperature and sexual differentiation. Perturbation of the SAS pathways not only impairs the ability of C. neoformans to resist a variety of environmental stresses during host infection, but also affects production of virulence factors, such as capsule and melanin. A drug(s) capable of targeting signaling components of the SAS pathway will be effective for treatment of cryptococcosis.

• Structural Engineering and Mechanics
• /
• v.37 no.1
• /
• pp.111-129
• /
• 2011

This paper presents a nonlinear analysis of reinforced concrete column with lap splice confined by FRP wrapping in the critical hinging zone. The steel stress-slip model derived from the tri-uniform bond stress model presented in the companion paper is included in the nonlinear frame analysis to simulate the response of reinforced concrete columns subjected to cyclic displacement reversals. The nonlinear modeling is based on a fiber discretization of an RC column section. Each fiber is modeled as either nonlinear concrete or steel spring, whose load-deformation characteristics are calculated from the section of fiber and material properties. The steel spring that models the reinforcing bars consists of three sub-springs, i.e., steel bar sub-spring, lap splice spring, and anchorage bond-slip spring connected in series from top to bottom. By combining the steel stress versus slip of the lap splice, the stress-deformation of steel bar and the steel stress-slip of bars anchored into the footing, the nonlinear steel spring model is derived. The analytical responses are found to be close to experimental ones. The analysis without lap splice springs included may result in an erroneous overestimation in the strength and ductility of columns.

• Asian-Australasian Journal of Animal Sciences
• /
• v.31 no.1
• /
• pp.47-53
• /
• 2018

Objective: The objective of this experiment was to investigate the effects of heat stress on milk protein and blood amino acid profile in dairy cows. Methods: Twelve dairy cows with the similar parity, days in milk and milk yield were randomly divided into two groups with six cows raised in summer and others in autumn, respectively. Constant managerial conditions and diets were maintained during the experiment. Measurements and samples for heat stress and no heat stress were obtained according to the physical alterations of the temperature-humidity index. Results: Results showed that heat stress significantly reduced the milk protein content (p<0.05). Heat stress tended to decrease milk yield (p = 0.09). Furthermore, heat stress decreased dry matter intake, the concentration of blood glucose and insulin, and glutathione peroxidase activity, while increased levels of non-esterified fatty acid and malondialdehyde (p<0.05). Additionally, the concentrations of blood Thr involved in immune response were increased under heat stress (p<0.05). The concentration of blood Ala, Glu, Asp, and Gly, associated with gluconeogenesis, were also increased under heat stress (p<0.05). However, the concentration of blood Lys that promotes milk protein synthesis was decreased under heat stress (p<0.05). Conclusion: In conclusion, this study revealed that more amino acids were required for maintenance but not for milk protein synthesis under heat stress, and the decreased availability of amino acids for milk protein synthesis may be attributed to competition of immune response and gluconeogenesis.