• Magazine of the Korean Society of Agricultural Engineers
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• v.38 no.5
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• pp.74-84
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• 1996

Dynamic loading of structures often causes excursions of stresses well into the inelastic range and the influence of geometric changes on the dynamic response is also significant in many cases. Therefore, both material and geometric nonlinearity effects should be considered in case that a dynamic load acts on the structure. For developing a program to analyze the dynamic response of an axisymmetric shell in this study, the material nonlinearity effect on the dynamic response was formulated by the elasto-viscoplastic model highly corresponding to the real behavior of the material. Also, the geometrically nonlinear behavior is taken into account using a total Lagrangian coordinate system, and the equilibrium equation of motion was numerically solved by a central difference scheme. A complete finite element program has been developed and the results obtained by it are compared with those in the references 1 and 2. The results are in good agreement with each other. As a case study of its application, the developed program was applied to a dynamic response analysis of a nuclear reinforced concrete containment structure. The results obtained from the' numerical examples are summarized as follows : 1. The dynamic magnification factor of the displacement and the stress were unrelated with the concrete strength. 2. As shown by the results that the displacement dynamic magnification factor were form 1.7 to 2.3 and the stress dynamic magnification factor from 1.8 to 2.5, the dynamic magnification factor of stress were larger than that of displacement. 3. The dynamic magnification factor of stress on the exterior surface was larger than that on the interior surface of the structure.

• Computers and Concrete
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• v.19 no.1
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• pp.59-68
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• 2017

This study focused on modeling the behavior of the compressive stress using the average strain and ultrasonic test results in concrete. Feed-forward backpropagation artificial neural network (ANN) models were used to compare four types of concrete mixtures with varying water cement ratio (WC), ordinary concrete (ORC) and concrete with short steel fiber-reinforcement (FRC). Sixteen (16) $150mm{\times}150mm{\times}150mm$ concrete cubes were used; each contained eighteen (18) data sets. Ultrasonic test with pitch-catch configuration was conducted at each loading state to record linear and nonlinear test response with multiple step loads. Statistical Spearman's rank correlation was used to reduce the input parameters. Different types of concrete produced similar top five input parameters that had high correlation to compressive stress: average strain (${\varepsilon}$), fundamental harmonic amplitude (A1), $2^{nd}$ harmonic amplitude (A2), $3^{rd}$ harmonic amplitude (A3), and peak to peak amplitude (PPA). Twenty-eight ANN models were trained, validated and tested. A model was chosen for each WC with the highest Pearson correlation coefficient (R) in testing, and the soundness of the behavior for the input parameters in relation to the compressive stress. The ANN model showed increasing WC produced delayed response to stress at initial stages, abruptly responding after 40%. This was due to the presence of more voids for high water cement ratio that activated Contact Acoustic Nonlinearity (CAN) at the latter stage of the loading path. FRC showed slow response to stress than ORC, indicating the resistance of short steel fiber that delayed stress increase against the loading path.

• The Korean Journal of Malacology
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• v.29 no.3
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• pp.181-187
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• 2013

In order to investigate environmental stress inducible genes in abalone, we analyzed differentially expressed transcripts from a Pacific abalone, Haliotis discus hannai, after exposure to heat-, cold- or hyposalinity-shock by suppression subtractive hybridization (SSH) method. 1,074 unique sequences from SSH libraries were composed to 115 clusters and 986 singletons, the overall redundancy of the library was 16.3%. From the BLAST search, of the 1,316 ESTs, 998 ESTs (75.8%) were identified as known genes, but 318 clones (24.2%) did not match to any previously described genes. From the comparison results of ESTs pattern of three SSH cDNA libraries, the most abundant EST was different in each SSH library: small heat shock protein p26 (sHSP26) in heat-shock, trypsinogen 2 in cold-shock, and actin in hyposalinity SSH cDNA library. Based on sequence similarities, several response-to-stress genes such as heat shock proteins (HSPs) were identified commonly from the abalone SSH libraries. HSP70 gene was induced by environmental stress regardless of temperature-shock or salinity-stress, while the increase of sHSP26 mRNA expression was not detected in cold-shock but in heat-shock condition. These results suggest that the suppression subtractive hybridization method is an efficient way to isolate differentially expressed gene from the invertebrate environmental stress-response transcriptome.

• Journal of the Korea Convergence Society
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• v.13 no.4
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• pp.185-194
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• 2022

This study tried to understand the mediating role of stress perception and the moderating role of mindfulness in the process of psychological alienation affecting college students' smartphone addiction tendency. Two hundred seventeen university students from Chungcheongnam-do answered the questionnaire. The research model was analyzed with model 7 of Hayes' PROCESS Macro 4.0. When synthesizing analysis results, feelings of alienation mediate stress responses or directly influence smartphone addiction tendencies. At this time, people with high levels of mindfulness tended to lower their smartphone addiction tendencies by alleviating their stress response compared to those with a low level of mindfulness. The implication of this study is that it revealed the psychological process that causes alienation to induce smartphone addiction tendency through stress responses, and that mindfulness plays a role in alleviating stress response and lowering smartphone addiction tendency. In the future, it is necessary to apply the model of this study to people diagnosed with smartphone addiction.

• Journal of Korean Academy of Nursing
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• v.48 no.3
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• pp.298-310
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• 2018

Purpose: The purpose of this study was to examine the effects of the laughter therapy program on perceived stress and psycho-neuro-endocrine-immune responses in obese women. Methods: A nonequivalent control group with a pretest-posttest design was used. The participants (n=60), whose age ranged from 30 to 50 years (pre-menopausal and body mass index of over $25kg/m^2$), were assigned to the experimental group (n=24) or control group (n=26). The experimental group was provided with the laughter therapy program (12 sessions) for 6 weeks. Results: There were significant differences in perceived stress, psychological stress response, fasting blood sugar, interleukin-6, and tumor necrosis factor alpha between the two groups after the program. However, there were no significant differences in normalized low frequency (norm LF), normalized high frequency (norm HF), LF/HF ratio, and cortisol between the two groups after the program. Conclusion: It was found that the laughter therapy program had positive effects on some variables in terms of perceived stress and psycho-neuro-endocrine-immuno responses. It is suggested that the laughter therapy in this study can provide the direction for developing a program for obese women.

• Molecules and Cells
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• v.21 no.3
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• pp.356-359
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• 2006

The transcription factor E2F1 coordinates cell cycle progression and induces apoptosis in response to DNA damage stress. Aside from DNA damage, the role of E2F1 in the endoplasmic reticulum (ER) stress signaling pathways is unclear. We found that $E2F1^{-/-}$ murine embryonic fibroblasts (MEFs) are resistant to apoptosis triggered by the ER stress inducer thapsigargin. In addition, E2F1 deficiency results in enhanced phosphorylation of eukaryotic translation initiation factor $2{\alpha}$ ($elF2{\alpha}$). These results therefore indicate that E2F1 deficiency increases phosphorylation of $elF2{\alpha}$ in response to ER stress triggered by thapsigargin, and suggest that the reduction in ER stress-induced apoptosis in E2F1-deficient cells is related to the high level of $elF2{\alpha}$ phosphorylation.

• Journal of Plant Biotechnology
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• v.50
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• pp.225-231
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• 2023

Cold stress is one of the most vulnerable environmental stresses that affect plant growth and crop yields. With the recent advancements in genetic approaches using Arabidopsis and other model systems, genes involved in cold-stress response have been identified and the key cold signaling factors have been characterized. Exposure to low-temperature stress triggers the activation of a set of genes known as cold regulatory (COR) genes. This activation process plays a crucial role in enhancing the resistance of plants to cold and freezing stress. The inducer of the C-repeatbinding factor (CBF) expression 1-CBF module (ICE1-CBF module) is a key cold signaling pathway regulator that enhances the expression of downstream COR genes; however, this signaling module in Panax ginseng remains elusive. Here, we identified cold-signaling-related genes, PgCBF1, PgCBF3, and PgICE1 and conducted functional genomic analysis with a heterologous system. We confirmed that the overexpression of cold- PgCBF3 in the cbf1/2/3 triple Arabidopsis mutant compensated for the cold stress-induced deficiency of COR15A and salt-stress tolerance. In addition, nuclearlocalized PgICE1 has evolutionarily conserved phosphorylation sites that are modulated by brassinsteroid insensitive 2 (PgBIN2) and sucrose non-fermenting 1 (SNF1)-related protein kinase 3 (PgSnRK3), with which it physically interacted in a yeast two-hybrid assay. Overall, our data reveal that the regulators identified in our study, PgICE1 and PgCBFs, are evolutionarily conserved in the P. ginseng genome and are functionally involved in cold and abiotic stress responses.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.10 no.1
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• pp.14-22
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• 2003

Purpose: To improve self-management in elderly people with hypertension, the researcher examined the effects of an abdominal relaxed breathing exercises using biofeedback on blood pressure. These exercises regulate physical response to stress and lessen the activity of sympathetic nerves. Method: A single-experimental pre-and post-test design was used. Eight elderly people with hypertension participated in the study from April, 6 to June, 14, 2000. Biofeedback was done with soft ware by developed by J & J Company (1-410 form for abdominal respiration training). The abdominal relaxed breathing exercises are based on Mason's suggestion (1985) and were modified by Yu & Song(2001). This program consisted of 4 sessions once a week for four weeks. Data were analysed using SPSSPC+(10.0). Result: There was a significant decrease in systolic and diastolic blood pressure over three different times. There was a significant difference in the level of total stress response, overall health and quality of life but no significant difference in the level of emotional condition over three different times. Conclusion: The results of this study will contribute to the development of nursing strategies for the regulation of blood pressure in older people as the exercises are easy to learn and are a nonpharmacologic approach.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.5
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• pp.725-732
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• 2020

The sudden drop of water temperature in winter is very threatening factor that affects the productivity of farmed fish and the management in aquafarm. In this study, we investigated the effect of low temperature on the survival, oxygen consumption and stress responses of parrotfish Oplegnathus fasciatus due to acute drop of water temperature. The survival rate of parrotfish Oplegnathus fasciatus was 5% at 6℃, 95% at 8℃ and 100% at 10℃ on the 4^th day of exposure in each experimental temperature. Low-lethal temperature for 4days of parrotfish Oplegnathus fasciatus (4 day-LT₅₀) was 6.99℃ (confidence limit, 6.55-7.42℃). Oxygen consumption rate was significantly decreased with decreasing water temperature. Temperature coefficient (Q₁₀) was found to be 4.0 between 10℃ and 8℃ and 0.39 between 8℃ and 6℃. As a result of investigating the stress response according to the drop in water temperature, the concentration of SOD (Superoxide dismutase), cortisol, glucose, total Ig, AST (Aspartate) and ALT (Alanine aminotransferase) increased with decreasing of water temperature. This study would be useful for the management of temperature about cultured fish.

• IMMUNE NETWORK
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• v.6 no.1
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• pp.6-12
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• 2006

Background: The Hypothalamo-Pituitary-Adrenal (HPA) axis is an important regulator for the body's stress response. As a primary stress responsive system, HPA-axis secretes various neurotransmitters, hormones, and cytokines, which regulates the immune system. Natural killer (NK) cell which is plays an important role in the innate immune response, is specially decreased their numbers and loose cytolytic activity in response to stress. However, the effect of HPA-axis secreted proteins on NK cell activity has not been defined. Herein, we studied the effect of adrenal secreted adiponectin on NK cell cytotoxicity. Adiponectin which is well-known metabolic control protein, plays important roles in various diseases, including hypertension, cardiovascular diseases, inflammatory disorders, and cancer. Methods: Signal sequence trap was used to find stress novel secretory protein from HP A-axis. Selected adiponectin was treated mouse mature primary NK cells and then examined the effect of adiponectin to NK cell cytotoxicity and cytokine expression level. Results: We found that adiponectin which is secreted from adrenal gland, suppress IL-2 induced NK cell cytotoxicity. And also investigated cytolytic cytokines are suppressed by adiponectin. Conclusion: These data suggest that adiponectin inhibites NK cell cytotoxicity via suppression of cytotoxicity related target gene.