• Title/Summary/Keyword: Stress-Fiber formation

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Phospholipase D Is Not Involved in Rho A-Mediated Activation of Stress Fiber Formation

  • Leem, Sun-Hee;Shin, In-Cheol;Kweon, Soo-Mi;Kim, Seung-Il;Kim, Jae-Hong;Ha, Kwon-Su
    • BMB Reports
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    • v.30 no.5
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    • pp.337-341
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    • 1997
  • In order to investigate the role of a small GTP-binding protein RhoA in lysophosphatidic acid (LPA)-induced stress fiber formation, C3 ADP-ribosyltransferase was prepared by expressing in E. coli and then applied to Rat-2 fibroblasts. C3 transferase isolated from E. coli was as effective as the toxin from Clostridium botulinum in ADP-ribosylation of RhoA. Incubation of the cells with C3 transferase for 2 days induced ADP-ribosylation of RhoA by a dose-dependent manner, with a sub-maximal induction at $25\;{\mu}g/ml$. As expected, LPA-induced stress fiber formation was completely blocked by pre-incubation with C3 transferase for 2 days. However, exogenously added C3 transferase had no significant effect on the formation of phosphatidylethanol by LPA. These results suggested that phospholipase D was not activated by RhoA in the LPA-induced stress fiber formation.

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The Reliability of Optical Fiber Assembly Using Glass Solder

  • Lee, Jong-Jing;Kang, Hyun-Seo;Koh, Jai-Sang
    • Proceedings of the Korean Reliability Society Conference
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    • 2004.07a
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    • pp.147-151
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    • 2004
  • In this study, an optical fiber assembly directly coupled with a laser diode or a photo diode is designed to confirm high reliable optical coupling efficiency of optical transmitter(Tx) and receiver(Rx). The optical fiber assembly is fabricated by soldering an optical fiber and a Kovar ferrule using a glass solder after inserting an optical fiber through a Kovar ferrule. The Kovar which has good welding characteristics is applied to introduce laser welding technique. The glass solder has excellent thermal characteristics such as thermal shift delamination compared with PbSn, AuSn solder previously used usually. Furthermore, the glass solder doesn't need fiber metalization and this enables low cost fabrication. However, the glass soldering is high temperature process over 35$0^{\circ}C$ and the convex shape after solidification due to surface tension causes the stress concentration on optical fiber. The stress concentration on the optical fiber increases the optical insertion loss and possibility of crack formation. The shape of glass solder was designed referring to 2-D Axi-symmetric FEM simulation. To test the mechanical reliability, mechanical vibration test and shock test were done according to Telcorida GR-468-Core protocol. After each test, the optical loss of the stress distributed fiber assembly didn't exceed 0.5 dB, which passes the test.

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The Effect of Etching on Low-stress Mechanical Properties of Polypropylene Fabrics under Helium/Oxygen Atmospheric Pressure Plasma

  • Hwang, Yoon J.;An, Jae Sang;McCord, Marian G.;Park, Shin Woong;Kang, Bok Choon
    • Fibers and Polymers
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    • v.4 no.4
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    • pp.145-150
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    • 2003
  • Polypropylene nonwoven fabrics were exposed to He/$O_2$ atmospheric pressure glow discharge plasma. Surface chemical analysis and contact angle measurement revealed the surface oxidation by formation of new functional groups after plasma treatment. Weight loss (%) measurement and scanning electron microscopy analysis showed a significant plasma etching effect. It was investigated in low-stress mechanical properties of the fabrics using Kawabata Evaluation System (KES-FB). The surface morphology change by plasma treatment increased surface friction due to an enhancement of fiber-to-fiber friction, resulting in change of other low-stress mechanical properties of fabric.

Strain Analysis of Composite Laminates Using Optical Fiber Sensor (광섬유센서를 이용한 복합적층판의 변형률 해석)

  • Woo S.C.;Choi N.S.;Park L.Y.;Kwon I.B.
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2004.04a
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    • pp.111-114
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    • 2004
  • Using the embedded optical fiber sensor of totally-reflected extrinsic Fabry-Perot interferometer(TR-EFPI), longitudinal strains(Ex) of the core and skin layers in glass fiber reinforced plastic(GFRP) cross-ply composite laminates have been measured. Transmission optical microscopy was employed to study the damage formation around the TR-EFPI sensor. It was observed that values of ex in the interior of the skin layer and the core layer measured by embedded TR-EFPI sensor was significantly higher than that of the specimen surface measured by strain gauges. The experimental results agreed well with those from finite element analysis on the basis of uniform stress model. Large strains in the core layer led to the occurrence of transverse cracks which drastically reduced the strain at failure of optical fiber sensor embedded in the core layer.

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Effects of Fluid Shear Stress on 3T3-L1 Preadipocytes (유체전단응력에 의하여 3T3-L1 지방세포가 받는 영향)

  • Lee, Jeongkun;Lee, Yeong Hun;Jin, Heewon;Lee, Seohyun;Kim, Chi Hyun
    • Journal of Biomedical Engineering Research
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    • v.39 no.4
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    • pp.168-174
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    • 2018
  • Adipocytes affect obesity through the regulation of lipid metabolism. Physical loading is an important regulator of fat tissue. There are ongoing in vitro studies inducing mechanotransduction on 3T3-L1 preadipocytes with mechanical stimulus in order to treat obesity by inhibiting adipogenesis and provoking cell death. In this study, our goal was to suggest a new therapy for obesity by investigating whether fluid shear stress (FSS) changes transcription factors on 3T3-L1 related with adipogenesis and cell death. FSS loading was applied to 3T3-L1 preadipocytes at 1Pa and 1Hz. After loading, bright field images were taken and an immunofluorescence assay was conducted to observe actin stress fiber formation. Western blot analysis was conducted to identify the activation of the ERK pathway as well as the adipogenic factors, which including C/EBPs and $PPAR{\gamma}$. The expression of osteopontin, a protein related to inflammation in adipose tissue, and cell death related factors, Bax, Bcl-2, and Beclin, were also measured. Results showed that FSS stimulated the formation of actin stress fibers in 3T3-L1 and also that the activation of C/EBPs decreased significantly when compared with the control group. $PPAR{\gamma}$ activation in the 2 hour FSS group was lower than the 1 hour FSS group, which implied that the results were time dependent. Additionally, there were no differences in the expression of cell death factors after FSS loading. In summary, similar to other fibroblasts, the formation of actin stress fibers induced by mechanotransduction may affect the differentiation of 3T3-L1, leading to inhibition of adipogenesis and inflammation.

Experiment and simulation analysis on full scale double-layer concrete shell

  • Thanh Quang Khai Lam;Thi My Dung Do
    • Computers and Concrete
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    • v.31 no.1
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    • pp.9-21
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    • 2023
  • The published studies usually used analytical method, numerical methods or experimental method to determine the stress-strain state and displacement of the single-layer or multi-layer curved shell types, but with a small scale model. However, a full scale multi-layer doubly curved concrete shell roof model should be researched. This paper presents the results of the experiment and simulation analysis involving stress-strain state, sliding between layers, the formation and development of the full scale double-layer doubly curved concrete shell roof when this shell begins to crack. The results of the this study have constructed the load-sliding strain relationship; strain diagram; stress diagram in the shell layers; the Nx, Ny membrane force diagram and deflection of shell. Thisresults by experimental method on a full scale model of concrete have clarified the working of multi-layer doubly curved concrete shell roof. The experimental and simulation results are compared with each other and compared with the Sap2000 software.

Algorithm of solving the problem of small elastoplastic deformation of fiber composites by FEM

  • Polatov, Askhad M.;Khaldjigitov, Abduvali A.;Ikramov, Akhmat M.
    • Advances in Computational Design
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    • v.5 no.3
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    • pp.305-321
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    • 2020
  • In this paper is presented the solution method for three-dimensional problem of transversely isotropic body's elastoplastic deformation by the finite element method (FEM). The process of problem solution consists of: determining the effective parameters of a transversely isotropic medium; construction of the finite element mesh of the body configuration, including the determination of the local minimum value of the tape width of non-zero coefficients of equation systems by using of front method; constructing of the stiffness matrix coefficients and load vector node components of the equation for an individual finite element's state according to the theory of small elastoplastic deformations for a transversely isotropic medium; the formation of a resolving symmetric-tape system of equations by summing of all state equations coefficients summing of all finite elements; solution of the system of symmetric-tape equations systems by means of the square root method; calculation of the body's elastoplastic stress-strain state by performing the iterative process of the initial stress method. For each problem solution stage, effective computational algorithms have been developed that reduce computational operations number by modifying existing solution methods and taking into account the matrix coefficients structure. As an example it is given, the problem solution of fibrous composite straining in the form of a rectangle with a system of circular holes.

Assessing interfacial fracture in orthotropic materials: Implementing the RIS concept with considering the T-stress term under mixed-mode I/II

  • Zahra Khaji;Mahdi Fakoor
    • Steel and Composite Structures
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    • v.50 no.2
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    • pp.237-247
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    • 2024
  • Research on interfacial crack formation in orthotropic bi-materials has experienced a notable increase in recent years, driven by growing concerns about structural integrity and reliability. The existence of a crack at the interface of bi-materials has a substantial impact on mechanical strength and can ultimately lead to fracture. The primary objective of this article is to introduce a comprehensive analytical model and establish stress relationships for investigating interfacial crack between two non-identical orthotropic materials with desired crack-fiber angles. In this paper, we present the application of the Interfacial Maximum Tangential Stress (IMTS) criterion, in combination with the Reinforcement Isotropic Solid (RIS) model, to investigate the behavior of interfacial cracks in orthotropic bi-materials under mixed-mode I/II loading conditions. We analytically characterize the stress state at the interfacial crack tip using both Stress Intensity Factors (SIFs) and the T-stress term. Orthotropic materials, due to their anisotropic nature, can exhibit complex crack tip stress fields, making it challenging to predict crack initiation behavior. The secondary objective of this study is to employ the IMTS criterion to predict the crack initiation angle and explore the notable impact of the T-stress term on fracture behavior. Furthermore, we validate the effectiveness of our approach in evaluating Fracture Limit Curves (FLCs) for interfacial cracks in orthotropic bi-materials by comparing our FLCs with relevant experimental data from existing literature.

Signals of MLCK and ROCK Pathways Triggered via Lymphotoxin β Receptor are Involved in Stress Fiber Change of Fibroblastic Reticular Cells (FRC에서 Lymphotoxin β receptor의 자극은 MLCK와 ROCK의 이중 신호전달 경로를 통해 stress fiber 변화에 관여)

  • Kim, Dae Sik;Lee, Jong-Hwan
    • Journal of Life Science
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    • v.29 no.2
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    • pp.256-264
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    • 2019
  • Lymphotoxin ${\beta}$ receptor ($LT{\beta}R$), a member of the tumor necrosis factor receptor family, plays an important role in lymphoid tissue's architecture and organogenesis. In contrast, MLCK and ROCK play critical roles in the regulation of stress fiber (SF) formation in cells. To determine whether $LT{\beta}R$ stimulation in fibroblastic reticular cells (FRCs) is involved in these signaling pathways, myosin light chain kinase inhibitor-7 (ML-7) was used to inhibit them. ML7-treated FRCs completely blocked SFs and showed retraction and shrinkage processes comparable to those observed in agonistic anti-$LT{\beta}R$ antibody-treated cells. The inhibition of ROCK activity with Y27632-induced changes in actin cytoskeleton organization and cell morphology in FRCs. Actin bundles rearranged into SFs, and phospho-myosin light chain (p-MLC) co-localized in FRCs. We checked the level of Rho-guanosine diphosphate (RhoGDP)/guanosine triphosphate (GTP) exchange activity using FRC lysate. When $LT{\beta}R$ was stimulated with agonistic anti-$LT{\beta}R$ antibodies, Rho-GDP/GTP exchange activity was markedly reduced. Regarding $LT{\beta}R$ signaling with a focus on MLCK inhibition, we showed that the phosphorylation of MLCs was reduced by $LT{\beta}R$ stimulation in FRCs. Cytoskeleton components, such as tubulin, b-actin, and phospho-ezrin proteins acting as membrane-cytoskeleton linkers, were produced in de-phosphorylation, and they reduced expression in agonistic anti-$LT{\beta}R$ antibody-treated FRCs. Collectively, the results suggested that MLCK and ROCK were simultaneously responsible for SF regulation triggered by $LT{\beta}R$ signaling in FRCs.

Helium/Oxygen Atmospheric Pressure Plasma Treatment on Poly(ethylene terephthalate) and Poly(trimethylene terephthalate) Knitted Fabrics: Comparison of Low-stress Mechanical/Surface Chemical Properties

  • Hwang Yoon Joong;McCord Marian G.;Kang Bok Choon
    • Fibers and Polymers
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    • v.6 no.2
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    • pp.113-120
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    • 2005
  • Helium-oxygen plasma treatments were conducted to modify poly(trimethylene terephthalate) (PIT) and poly(ethylene terephthalate) (PET) warp knitted fabrics under atmospheric pressure. Lubricant and contamination removals by plasma etching effect were examined by weight loss $(\%)$ measurements and scanning electron microscopy (SEM) analysis. Surface oxidation by plasma treatments was revealed by x-ray photoelectron spectroscopy (XPS) analyses, resulting in formation of hydrophilic groups and moisture regain $(\%)$ enhancement. Low-stress mechanical properties (evaluated by Kawabata evaluation system) and bulk properties (air permeability and bust strength) were enhanced by plasma treatment. Increasing interfiber and interyarn frictions might play important roles in enhancing surface property changes by plasma etching effect, and then changing low-stress mechanical properties and bulk properties for both fabrics.