• 대한한의학회지
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• 제26권1호
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• pp.174-186
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• 2005

Objectives : The water extract of Samul-tang (SMT) has traditionally been used for treatment of ischemic heart and brain damage in oriental medicine. However, little is known about the mechanism by which the water extract of SMT rescues cells from these damages. Methods: This study was designed to investigate the protective mechanisms of SMT on oxidative stress-induced toxicity in H9c2 cardiomyoblast cells. Treatment with $H_2O_2$ markedly induced death of H9c2 cardiomyoblast cells in a dose-dependent manner. Results: The characteristics of H20z-induced death of H9c2 showed apparent apoptotic features such as DNA fragmentation and morphological change. However, SMT significantly reduced both H202-induced cell death and morphological change. The decrease of Bc-2 expression by High were inhibited by SMT. In addition, the increase of Bax expression was also inhibited by SMT. The cotreatment of SMT and $H_2O_2$ in H9c2 cells also induced the phosphorylation of ERK in a time-dependent manner. Moreover, PD98059, a specific inhibitor of ERK1/2 attenuated the protective effects of SMT on $H_2O_2-induced$ toxicity in H9c2 cardiomyoblast cells. These results suggest that both ERK1/2 signaling pathways play important roles in the protective effects of SMT on $H_2O_2-induced$ apoptotic death of H9c2 cells. Also, the expression profile of proteins in $H_2O_2$ cardiomyoblast cells were screened by using two-dimensional (2-D) gel electrophoresis. Among 300 spots resolved in 2-D gels, the comparison of control versus apoptosis cells revealed that signal intensity of 17 spots increased and 11 spots decreased. Conclusions: Taken together, this study suggests that the protectiw effects of the water extract of SMT against oxidative damages may be mediated by the modulation of Bc1-2 and Bax expression via the regulation of the ERK signaling pathway.

• Journal of Plant Biotechnology
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• 제32권2호
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• pp.97-103
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• 2005

Superoxide dismutase와 ascorbate peroxidase가 동시에 과대발현된 담배(CA)를 가지고 여러 제초제들에 대한 항산화 반응을 조사하였다. 온실조건 실험에서, CA 담배는 PSI 저해제로 알려진 paraquat처리에 대해서 내성이 인정되었고 그 정도는 40% 내외였다. PS II 저해제 (bromoxynil, diuron, bromacil), 엽록소 생합성 저해제 (oxyfluorfen), 카로티노이드 생합성 저해제 (fluridone)와 EPSP synthase 저해제 (glyphosate) 처리에서는 CA와 wild type간의 반응차이가 관찰되지 않았다. Paraquat와 diuron을 이용한 약광 조건의 실험에서도 온실조건의 실험결과와 유사한 정도로 paraquat처리에 대해서만 내성을 나타내었다. 온실조건에서의 엽위별 반응의 경우, 6 - 9 엽기 식물체에 paraquat를 처리하였을 때, 약제처리 당시 위로부터 3 - 4번째 전개되고 있었던 잎이 상대적으로 내성 정도가 높게 나타났다. 한편 paraquat 처리 시에 여러 농도의 ascorbic acid를 혼합할 경우, CA와 wild type 모두에서 비슷한 정도로 paraquat 활성을 경감시켰다. 결론적으로, CuZnSOD/APX의 과대발현은 photosystem I 에서 발생되는 산화스트레스에 대해서만 주로 작용하며, 다른 제초제들에 의해 발생되는 산화적 스트레스에 대해서는 소거 능력이 부족한 것으로 판단되었다.

• 대한기계학회논문집A
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• 제40권12호
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• pp.1005-1011
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• 2016

기어소음의 근본적인 원인은 전달오차로 인해 발생하게 된다. 전달오차는 기어가 맞물릴 때 발생하는데 크게 정적전달오차와 동적전달오차가 있다. 이러한 오차들은 기어 이빨의 처짐 또는 치면 마찰에 의해 발생하고 이 요인들이 원인이 되어 기어 시스템에 진동이 발생하게 된다. 그리고 이 진동이 기어의 축으로 이동하게 되어 축을 떠받치고 있는 베어링에 전달되고 베어링에 전달된 가진은 최종적으로 기어의 케이싱으로 이동하게 되어 소음을 방출하게 된다. 본 논문에서는 이러한 전달오차에 의해 발생하는 최대 굽힘응력을 가지는 롤각을 찾기 위한 응력해석을 수행되었다. 본 논문에서는 이론적 바탕으로 설계되어 인볼류트 곡선을 가진 기어와 수정된 치형을 가진 기어의 전달오차에 대한 해석을 진행하였다. 또한, 급작스러운 작동이나 큰 백래쉬로 인해 발생하는 충격강도에 대한 영향을 알아보기 위해 유한요소해석을 통해 각각 정적 최대굽힘응력과 동적 최대굽힘응력의 결과를 이용하여 충격인자 값을 예측해 보았다.

• 전기학회논문지
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• 제61권9호
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• pp.1340-1349
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• 2012

Increasing the oxidation of fat through exercise is the recommendable method for weight control. Preceding researches have proposed increase in the usage of fat during exercise in stabilized state and under maximum exertion through aerobic training. However, such researches require additional equipment for gas analysis in order to measure the caloric value or gas exchange of subjects during exercise. Such equipments become highly restrictive for those exercise and cause substantially higher cost. According to this, we have presented the method of estimating the maximal fat oxidation point through changes in LF & HF which reflects changes in heart rate and the autonomic nervous system in order to induce exercise for a less restrictive and efficient fat oxidation than existing methods. We have conducted exercise stress test on subject with similar exercise abilities, and have detected the changes in heart rate and changes in LF & HF by measuring changes in fat oxidation and measuring ECG signals at the same time through a gas analyzer. Changes in heart rate and HRV of the subjects during exercising was detected through only the electrocardiographic signals from exercising and detected the point of maximum fat oxidation that differs from person to person. The experiment was carried out 16 healthy males, and used Modified Bruce Protocol, which is one of the methods of exercise stress test methods that use treadmill. The fat oxidation amount during exercise of all the subjects showed fat oxidation of more than 4Fkcal/min in the exercise intensity from about 5 minutes to 10 minutes. The correlation between the maximal fat oxidation point obtained through gas analysis and the point when 60% starts to be relevant in the range from -0.01 to 0.01 seconds for values of R-R interval from changes in heart rate had correlation coefficients of 0.855 in Kendall's method and in Spearman's rho, it showed significant results of it being p<0.01 with 0.950, respectively. Furthermore, in the changes in LF & HF, we have determined the point where the normalized area value starts to become the same as the maximal fat oxidation point, and the correlation here showed 0.620 in Kendall and 0.780 in Spearma of which both showed significant results as p<0.01.

• 한국표면공학회지
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• 제53권1호
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• pp.1-8
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• 2020

Sulfamate-chloride baths were fabricated to study the properties of the electrodeposited Ni and NiCo thin films. The dependences of current efficiency, deposit composition of Ni and Co, residual stress, surface morphology and microstructure of electrodeposited Ni and NiCo thin films on CoCl₂ concentration in sulfamate-chloride baths were investigated. The current efficiency was measured to be more than about 90%, independent of the changes of CoCl₂ concentration in the baths. Residual stress of Ni and NiCo thin films was increased from about 45 to about 250 MPa with varying CoCl₂ concentration from 0 to 0.210 M CoCl₂ in the baths and then reached to a plateau, about 250 MPa above 0.420 M CoCl₂ concentration. Nodular surface morphologies were observed at most CoCl₂ concentrations in the baths except 0.210 M. NiCo thin film electrodeposited from the bath with 0.210 M CoCl₂ concentration showed an acicular surface morphology. Pure Ni thin film consists of FCC(111), FCC(200), FCC(220), and FCC(311) peaks without any preferred orientation. On the other hand NiCo thin films make up of HCP(100), FCC(111), HCP(101), FCC(200), FCC(220) or HCP(110), FCC(311) or HCP(112) and FCC(222) peaks. It was revealed from the analysis of XRD result that FCC(111) peak at the NiCo thin film electrodeposited from the bath with 0.084 M CoCl₂ concentration can be regarded as the preferred orientation. However the peak of the preferred orientation was changed to FCC(220) or HCP(110) above 0.084 M CoCl₂ concentration in the baths. Then the intensity of FCC(220) or HCP(110) peak was gradually decreased with increasing CoCl₂ concentration further. The crystalline size of pure Ni thin film was observed to be about 53 ㎛ and those of NiCo thin films were in the range of 35~45 ㎛.

• Nuclear Engineering and Technology
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• 제52권9호
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• pp.2092-2099
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• 2020

Many damages due to flow-accelerated corrosion and cracking have been observed during recent in-service inspections of nuclear power plants. To determine the operability or repair for damaged pipes, an integrity evaluation related to the damaged piping system should be performed by using already proven code and standards. One of them, the ASME Code Case is most popularly used to integrity assessment in nuclear power plants. However, the recent version of CC N-513 still recommends the simplified method which means a damaged elbow is assumed as an equivalent straight pipe. In addition, to enhance the accuracy integrity assessment in elbow, several previous studies recommend that the SIF and elastic COD values for an elbow with relatively large crack could be predicted by an interpolation technique. However, those estimates for elbow with relatively large crack might be derived to inaccurate results for crack growth analysis, such as for the allowable crack size and life estimation. Therefore, in this paper, the effect of crack length (0.3≤θ₁/π≤0.5) on SIF and elastic COD for elbow is systematically investigated. Then, for large crack in elbow, accurate estimates for SIF and elastic COD, which are widely used to assess the integrity of elbows, are proposed. Those proposed solutions are expected to be the technical basis for revisions of CC N-513-4 through the validation.

• Smart Structures and Systems
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• 제21권4호
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• pp.421-433
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• 2018

Carbon fiber reinforced polymer (CFRP) cable has good mechanical properties and corrosion resistance. However, the anchorage of CFRP cable is a big issue due to the anisotropic property of CFRP material. In this article, a high-efficient bonding anchorage with novel configuration is developed for CFRP cables. The acoustic emission (AE) technique is employed to evaluate the performance of anchorage in the fatigue test and post-fatigue ultimate bearing capacity test. The obtained AE signals are analyzed by using a combination of unsupervised K-means clustering and supervised K-nearest neighbor classification (K-NN) for quantifying the performance of the anchorage and damage evolutions. An AE feature vector (including both frequency and energy characteristics of AE signal) for clustering analysis is proposed and the under-sampling approaches are employed to regress the influence of the imbalanced classes distribution in AE dataset for improving clustering quality. The results indicate that four classes exist in AE dataset, which correspond to the shear deformation of potting compound, matrix cracking, fiber-matrix debonding and fiber fracture in CFRP bars. The AE intensity released by the deformation of potting compound is very slight during the whole loading process and no obvious premature damage observed in CFRP bars aroused by anchorage effect at relative low stress level, indicating the anchorage configuration in this study is reliable.

• 한국추진공학회지
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• 제19권6호
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• pp.26-32
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• 2015

본 연구에서는 파괴인성에 대하여 입자강화 복합재 추진제의 균열저항 특성을 분석하기 위하여 평가되었다. $50^{\circ}C$에서 $-60^{\circ}C$까지 온도 범위에서 WST 시험편을 이용하여 파괴인성시험이 수행되었다. 파괴인성에 대한 평가방법은 선형탄성파괴역학에 기초한 ASTM E399를 이용하여 산출하였다. 추진제의 분열하중과 응력확대계수는 온도가 내려감에 따라 증가하고 있다. 또한 디지털 이미지 상관법을 이용하여 가시화된 변형률장은 온도가 $50^{\circ}C$에서 $-40^{\circ}C$로 내려갈수록 증가하나, $-60^{\circ}C$에서 변형률장은 취성거동으로 인해 크게 감소한다.

• Composites Research
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• 제17권1호
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• pp.10-17
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• 2004

본 연구에서는 이관능성 에폭시(DGEBA)와 polymethylmethacrylate(PMMA)를 블랜딩하여 열적 특성과 기계적 계면특성을 측정하였다. 열적 특성은 DSC, DMA, 그리고 TGA를 이용하였으며, 블랜드의 기계적 계면특성을 측정하기 위해 contact angle로 표면자유에너지를 조사하였고, 파괴인성은 $K_{IC}$로 측정하고 $K_{IC}$ 실험 후 파괴단면을 SEM을 이용하여 관찰하였다. 실험 결과, 경화 온도와 유리전이 온도는 PMMA의 첨가에 의해 증가하는 것을 확인할 수 있었다. 또한 블랜드의 표면자유에너지는 PMMA의 저함량에서 높은 값을 나타내었고, 이는 비극성 요소의 증가와 극성 요소의 존재에 의한 것으로 판단된다. 블랜드의 파괴인성 측정 결과 5 phr에서 최대값을 나타내었다. 이는 DGEBA/PMMA 간의 상용성 또는 거대분자 사슬에서 물리적 결합의 증가에 기인하는 것으로 판단된다.

• 대한기계학회논문집A
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• 제26권11호
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• pp.2305-2311
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• 2002

The pressure tube is a major component of the CANDU reactor, which supports nuclear fuel bundle and heavy water coolant. Pressure tubes are installed horizontally inside the reactor and only selected samples are periodically examined during in-service inspection. In this respect, a probabilistic safety assessment method is more appropriate fur the assessment of overall pressure tube safety. The failure behavior of CANDU pressure tubes, however, is governed by delayed hydride cracking which is the major difference from pipings and reactor pressure vessels. Since the delayed hydride cracking has more widely distributed governing parameters, it is impossible to apply a general PFM methodology directly. In this paper, a PFM methodology for the safety assessment of CANDU pressure tubes is introduced by applying Monte Carlo simulation in determining failure probability Initial hydrogen concentration, flaw shape and depth, axial and radial crack growth rate and fracture toughness were considered as probabilistic variables. Parametric study has been done under the base of pressure tube dimension and hydride precipitation temperature in calculating failure probability. Unstable fracture and plastic collapse are used for the failure assessment. The estimated failure probability showed about three-order difference with changing dimensions of pressure tube.