• Title/Summary/Keyword: Stress indicators

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Inclusion of bioclimatic variables in genetic evaluations of dairy cattle

  • Negri, Renata;Aguilar, Ignacio;Feltes, Giovani Luis;Machado, Juliana Dementshuk;Neto, Jose Braccini;Costa-Maia, Fabiana Martins;Cobuci, Jaime Araujo
    • Animal Bioscience
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    • v.34 no.2
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    • pp.163-171
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    • 2021
  • Objective: Considering the importance of dairy farming and the negative effects of heat stress, more tolerant genotypes need to be identified. The objective of this study was to investigate the effect of heat stress via temperature-humidity index (THI) and diurnal temperature variation (DTV) in the genetic evaluations for daily milk yield of Holstein dairy cattle, using random regression models. Methods: The data comprised 94,549 test-day records of 11,294 first parity Holstein cows from Brazil, collected from 1997 to 2013, and bioclimatic data (THI and DTV) from 18 weather stations. Least square linear regression models were used to determine the THI and DTV thresholds for milk yield losses caused by heat stress. In addition to the standard model (SM, without bioclimatic variables), THI and DTV were combined in various ways and tested for different days, totaling 41 models. Results: The THI and DTV thresholds for milk yield losses was THI = 74 (-0.106 kg/d/THI) and DTV = 13 (-0.045 kg/d/DTV). The model that included THI and DTV as fixed effects, considering the two-day average, presented better fit (-2logL, Akaike information criterion, and Bayesian information criterion). The estimated breeding values (EBVs) and the reliabilities of the EBVs improved when using this model. Conclusion: Sires are re-ranking when heat stress indicators are included in the model. Genetic evaluation using the mean of two days of THI and DTV as fixed effect, improved EBVs and EBVs reliability.

Effects of Stocking Density on the Growth, Body Composition, Stress, Antioxidant Status and Immune Responses of Juvenile Rainbow Trout Oncorhynchus mykiss (사육 밀도가 무지개송어(Oncorhynchus mykiss) 치어의 성장, 체조성, 스트레스, 항산화 및 면역반응에 미치는 영향)

  • Jae-Hoon Kim;In Joon Hwang;Jin Woo Park;Jeong-Hyeon Cho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.1
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    • pp.79-88
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    • 2023
  • A 12-week feeding trial was conducted to evaluate the effect of different stocking densities on the growth, body composition, stress, antioxidant status, and immune responses of juvenile rainbow trout Oncorhynchus mykiss. Juvenile rainbow trout (32.2±0.5 g) were randomly placed in eight experimental tanks, each containing 2,000 L of water, at stocking densities of 150, 300, 600, and 1,200 individual/m3. The trout were assigned to four treatments (2.4, 4.8, 9.7, and 19.4 kg/m3) in duplicates. Following after the feeding trial, the fish cultured in the less dense group showed significantly higher growth and survival (P<0.05). In terms of proximate composition, the whole body crude protein and lipid contents were lower in the denser group (19.4 kg/m3). Regarding the antioxidant and immune responses, the fish reared in the relatively denser group exhibited lower lysozyme, immunoglobulin M, and superoxide dismutase levels. The stress indicators, glucose and cortisol, were significantly lower in the less dense group (2.4 kg/m3), while the denser group (19.4 km/m3) exhibited higher AST and ALP levels(P<0.05). The results showed that denser stocking has a crowding stress effect and negatively impacts growth and immune responses.

The Effect of Driving Specific Characteristics and Life Stress on Traffic Fafety (운전 상황에서의 개인특성과 생활스트레스가 교통안전에 미치는 영향)

  • Suran Lee ;EunKyoung Chung ;JaeYoung Kwon ;Young Woo Sohn
    • Korean Journal of Culture and Social Issue
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    • v.17 no.3
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    • pp.305-320
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    • 2011
  • The objectives of the present research are twofold. First, this research aims to compare the effect of trait characteristics(sensation seeking, social resistance and type-A behavior) with that of driving specific characteristics(driving anger and type-A driving) on problematic driving behavior. Second, the role of life stress as a mediator in the relationships between general trait characteristics and traffic safety index was examined. 1158 licensed commercial vehicle drivers were surveyed and their accident-related records were obtained in this research. Results showed that driving specific characteristics were significant indicators of traffic safety and life stress mediated the relationships between general trait characteristics and traffic safety index. These findings implicate that understanding drivers' driving specific characteristics and their levels of life stress is important to reduce problematic driving behaviors and enhance traffic safety.

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Effects of Short-term Acute Heat Stress on Physiological Responses and Heat Shock Proteins of Hanwoo Steer (Korean Cattle)

  • Baek, Youl-Chang;Kim, Minseok;Jeong, Jin-Young;Oh, Young-Kyoon;Lee, Sung-Dae;Lee, Yoo-Kyung;Ji, Sang-Yun;Choi, Hyuck
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.3
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    • pp.173-182
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    • 2019
  • This study was performed to evaluate the effect of heat stress on the status of physiological responses, blood parameter, serum T3 and cortisol, and heat shock proteins (HSP 27, 70, and 90) of Hanwoo cattle. Six Hanwoo steers (242.8 ± 7.2 kg of BW) were housed in the climate-controlled respiration chambers. The experiment consisted of 7 days (control; 0 day) at thermoneutral (air temperature (Ta) of 15℃ and relative humidity (RH) of 60%; temperature-humidity index (THI) = 64), and by 3 and 6 days (treatment groups) at heat stress (Ta of 35℃ and RH of 60%; THI = 87). Body temperature of each parts (frank, rump, perineum and foot) and rectal temperature elevated in heat stress groups (3 days and 6 days) than the control group (0 day). Respiration rates increased in 3 days and 6 days (88.5 ± 0.96 bpm and 86.3 ± 0.63 bpm, respectively) from 0 days (39.5 ± 0.65 bpm). Feed intake significantly decreased in heat stress groups (3 days and 6 days, 3.7 ± 0.14 kg and 4.0 ± 0.15 kg, respectively) than the control group (0 day, 5.0 ± 0.00 kg). In addition, final BW significantly decreased in heat stress groups (3 days and 6 days, 211.8 ± 4.75 kg and 215.5 ± 3.50 kg, respectively) than the control group (0 day, 240.0 ± 25.00 kg). However, heat stress has no significant effect on blood parameter, serum T3 and cortisol. Nevertheless, heat stress increased HSPs mRNA expression in liver tissue, and serum concentration of HSPs. Despite Hanwoo cattle may have high adaptive ability to heat stress, our results suggested that heat stress directly effect on body temperature and respiration rate as well as serum and tissue HSPs. Therefore, we are recommended that HSPs could be the most appropriate indicators of Hanwoo cattle response to heat stress.

Measuring stress responses using active biotelemetry in cattle I. Restraint stress responses in heifers and cows (Active biotelemetry를 이용한 젖소의 스트레스 반응 측정 I.보정 스트레스에 대한 경산우와 미경산우의 생체반응의 변화)

  • Lim, Joa-jin;Lee, Byeong-han;Kim, Jin-young;Lee, Su-han;Lee, Dong-hee;Kim, Jae-kyung;Jeong, Soon-wuk;Choe, Nong-hoon;Chung, Byung-hyun
    • Korean Journal of Veterinary Research
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    • v.41 no.4
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    • pp.597-602
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    • 2001
  • This study was carried out to investigate the effectiveness of active biotelemetry to monitor stress response, and the effects of acute stressor (raising a hind foot of a standing animal) on body temperature (BT) and heart rate (HR) in comparison heifer group (n=4) with cow group (n=3). Both parameters were recorded by active telemetry. Cortisol assay was used to compare the result of active biotelemetry. The stressor consisted in restraining the cows for hoof treatments. Body temperature and heart rate were measured at 0, 10, 20, 30, 40, 50, 60, 90, 120, 180 and 360 minutes during the experiments, and also cortisol assay at 0, 0.5, 1, 2, 3, 12 and 24 hours. Base line for these animals was set in there accustomed environment at -24hrs and -30 mins before the beginning of experiments. The results obtained in this study were as follows: 1. In the BT, heifer group showed no significant difference from cow group, but were observed slightly higher BT than cow group during restraint stress for 30 minutes. 2. The both groups showed directly significant increase of HR immediately after the beginning of the restraint stress (P<0.01), but in the required time for the recovery of HR after finishing the stress, heifer group was 40 minutes and cow group 60 minutes. 3. In the cortisol assay, the both groups increased from 30 to 120 minutes after the beginning of experiment (i.e, from 0 to 90 minutes after finishing the restraint stress), showing significant difference from before the beginning of the stress (P<0.01), but heifer group showed great increase of the cortisol level from 30 to 60 minutes after the beginning of the stress, while cow group was observed at 60 minutes. In conclusion, we suppose that care is required to decrease psychological stress in cattle when they suffer from a series of procedures for diagnosis and treatment, especially in heifers rather than in cows. Also BT and HR obtained by active biotelemetry are reliable indicators of stress in cattle.

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The Availability of Amplitude, Asymmetry and Mean Frequency of Alpha Intrinsic Rhythm in Old Age as Brain Health Indicators (노년기 알파 고유리듬 진폭, 비대칭 및 평균 주파수의 뇌건강 지표로써 활용 가능성 연구)

  • Shim, Jun-Young
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.2
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    • pp.124-132
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    • 2020
  • This study examines the clinical availability of alpha amplitude, asymmetry and mean frequency in old age as EEG indexes reflecting brain health condition. The EEGs of prefrontal lobes in eye closure state were measured for 4 minutes with 72 male and female elderly people aged 73. Subjects were divided into higher and lower groups based on average values of alpha amplitude, asymmetry and mean frequency. The results of correlations and t-test among EEG indexes were as follows. First, the higher Alpha altitude was, the higher physical and mental stress resistance and left-to-right brain communication were, and the lower mean Alpha mean frequency location was. The higher Alpha asymmetry was, the lower left-to-right communication level was. Second, as Alpha power of right brain was higher than that of left brain, Alpha amplitude and mental stress resistance and left-to-right brain communication level were higher. Third, as Alpha mean frequency location was higher, Alpha amplitude and mental anti-stress level were lowered and arousal level was enhanced. These high correlations and complementary meaning among quantitative EEG indicators demonstrated the possibility of biomarkers reflecting brain health state in old age.

Effect of knife castration on leukocyte cytokine expression and indicators of stress, pain, and inflammation in Korean cattle bull calves

  • Seonpil Yoo;Seok-Hyun Beak;Hyeok Joong Kang;Da Jin Sol Jung;Dilla Mareistia Fassah;InHyuk Jeong;Seung Ju Park;Md Najmul Haque;Myunghoo Kim;Myunggi Baik
    • Animal Bioscience
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    • v.36 no.3
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    • pp.521-528
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    • 2023
  • Objective: This study investigated the effects of surgical castration on behavior, physiological and inflammatory indicators, and leukocyte cytokine mRNA levels in Korean cattle bull calves. Methods: Nineteen Korean cattle bull calves (average body weight, 254.5 kg; average age, 8.2 months) were divided into two treatment groups: control (n = 9) and castration (n = 10). Surgical castration was performed using Newberry knives and a Henderson castrating tool. Blood was obtained just before castration (0 h) and at 0.5 h, 6 h, 1 d, 3 d, 7 d, and 14 d after castration. Plasma cortisol (PC), saliva cortisol (SC), plasma substance P, and plasma haptoglobin concentrations, and the leucocyte mRNA levels of the interleukin-1-alpha (IL1A), interleukin-1-beta (IL1B), interleukin-1 receptor antagonist (IL1RN), and interleukin-6 (IL6) genes were analyzed. Results: Castration decreased (p<0.01) the average daily gain and gain/feed ratio. Castration reduced the time spent eating (p<0.001) and the eating frequency (p<0.01) and increased (p<0.001) the lying frequency. Castration temporarily increased (p<0.05) circulating PC and SC concentrations at 0.5 h after castration. Castration temporarily increased (p<0.05) plasma substance P concentrations at 1 d after castration. Castration increased (p<0.05) plasma haptoglobin concentrations at 1 and 3 d after castration. Castration increased (p<0.05) leukocyte mRNA levels of the IL1A, IL1B, IL1RN, and IL6 genes at 6 h after castration. Conclusion: Castration temporarily induced stress and expression of leucocyte inflammatory cytokine genes in Korean cattle bull calves.

Socioeconomic Differentials in Health and Health Related Behaviors: Findings from the Korea Youth Panel Survey (사회경제적 위치에 따른 청소년의 건강과 건강 관련 행태의 차이 : 한국청소년패널 조사 결과)

  • Cho, Sung-Il;Yang, Seung-Mi;Lee, Moo-Song;Khang, Young-Ho
    • Journal of Preventive Medicine and Public Health
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    • v.38 no.4
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    • pp.391-400
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    • 2005
  • Objective : This study examined the socioeconomic differentials for the health and health related behaviors among South Korean middle school students. Methods : A nationwide cross-sectional interview survey of 3,449 middle school second-grade students and their parents was conducted using a stratified multi-stage cluster sampling method. The response rate was 93.3%. The socioeconomic position indicators were based on self-reported information from the students and their parents: parental education, father's occupational class, monthly family income, out-of-pocket expenditure for education, housing ownership, educational expectations, educational performance and the perceived economic hardships. The outcome variables that were measured were also based on the self-reported information from the students. The health measures included self-rated health conditions, psychological or mental problems, the feelings of loneliness at school, the overall satisfaction of life and the perceived level of stress. The health related behaviors included were smoking, alcohol drinking, sexual intercourse, violence, bullying and verbal and physical abuse by parents. Results : Socioeconomic differences for the health and health related behaviors were found among the eighth grade boys and girls of South Korea. However, the pattern varied with gender, the socioeconomic position indicators and the outcome measures. The prevalence rates of the overall dissatisfaction with life for both genders differed according to most of the eight socioeconomic position indicators. All the health measures were significantly different according to the perceived economic hardship. However, the socioeconomic differences in the self-rated health conditions and the psychosocial or mental problems were not clear. The students having higher socioeconomic position tended to be a perpetrator of bullying while those students with lower socioeconomic position were more likely to be a victim. Conclusions : The perceived economic hardships predicted the health status among the eighth graders of South Korea. The overall satisfaction of life was associated with the socioeconomic position indicators. Further research efforts are needed to explore the mechanisms on how and why the socioeconomic position affects the health and health related behaviors in this age group.

Evaluation on the stress using HRV according to elapsed time of MRI noise (HRV를 이용한 자기공명영상 소음의 시간 변화에 따른 스트레스 평가)

  • Ye, Soo-Young;Kim, Dong-Hyun
    • Journal of the Institute of Convergence Signal Processing
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    • v.16 no.2
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    • pp.50-55
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    • 2015
  • The noise of MRI shooting is 100dB loud and has an intensive psychological and physiological influences on the human body. ECG signals were measured by experimental methods, while wearing earplugs for 15 minutes in the stable state. Then the ECG signals were measured for 30 minutes while listening to about 100dB of sound in a MRI equipment. In this study, the heart rate variability of men and women was analyzed according to the MRI noise stress level through the frequency analysis. As the MRI noise level is about 100dB, HRV analysis resulted in an imbalance between the sympathetic and parasympathetic. During the period from the resting state up to 10 minutes, the maximum stress state was shown. This study will encourage MRI workers to take interests in hearing protection for the patient and to make objective indicators about MRI noises.

Pomegranate (Punica granatum) Peel Extract Efficacy as a Dietary Antioxidant against Azoxymethane-Induced Colon Cancer in Rat

  • Waly, Mostafa I.;Ali, Amanat;Guizani, Nejib;Al-Rawahi, Amani S.;Farooq, Sardar A.;Rahman, Mohammad S.
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.8
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    • pp.4051-4055
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    • 2012
  • Functional foods include antioxidant nutrients which may protect against many human chronic diseases by combating reactive oxygen species (ROS) generation. The purpose of the present study was to investigate the protective effect of pomegranate peel extract (PPE) on azoxymethane (AOM)-induced colon tumors in rats as an in vivo experimental model. Forty Sprague-Dawley rats (4 weeks old) were randomly divided into 4 groups containing 10 rats per group, and were treated with either AOM, PPE, or PPE plus AOM or injected with 0.9% physiological saline solution as a control. At 8 weeks of age, the rats in the AOM and PPE plus AOM groups were injected with 15 mg AOM/kg body weight, once a week for two weeks. After the last AOM injection, the rats were continuously fed ad-libitum their specific diets for another 6 weeks. At the end of the experiment (i.e. at the age of 4 months), all rats were killed and the colon tissues were examined microscopically for lesions suspected of being preneoplastic lesions or tumors as well as for biochemical measurement of oxidative stress indices. The results revealed a lower incidence of aberrant crypt foci in the PPE plus AOM administered group as compared to the AOM group. In addition, PPE blocked the AOM-induced impairment of biochemical indicators of oxidative stress in the examined colonic tissue homogenates. The results suggest that PPE can partially inhibit the development of colonic premalignant lesions in an AOM-induced colorectal carcinogenesis model, by abrogating oxidative stress and improving the redox status of colonic cells.