Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of knife castration on leukocyte cytokine expression and indicators of stress, pain, and inflammation in Korean cattle bull calves

  • Seonpil Yoo (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Seok-Hyun Beak (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Hyeok Joong Kang (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Da Jin Sol Jung (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Dilla Mareistia Fassah (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • InHyuk Jeong (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Seung Ju Park (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Md Najmul Haque (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Myunghoo Kim (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Myunggi Baik (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University)
  • Received : 2022.09.20
  • Accepted : 2022.11.16
  • Published : 2023.03.01
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Abstract

Objective: This study investigated the effects of surgical castration on behavior, physiological and inflammatory indicators, and leukocyte cytokine mRNA levels in Korean cattle bull calves. Methods: Nineteen Korean cattle bull calves (average body weight, 254.5 kg; average age, 8.2 months) were divided into two treatment groups: control (n = 9) and castration (n = 10). Surgical castration was performed using Newberry knives and a Henderson castrating tool. Blood was obtained just before castration (0 h) and at 0.5 h, 6 h, 1 d, 3 d, 7 d, and 14 d after castration. Plasma cortisol (PC), saliva cortisol (SC), plasma substance P, and plasma haptoglobin concentrations, and the leucocyte mRNA levels of the interleukin-1-alpha (IL1A), interleukin-1-beta (IL1B), interleukin-1 receptor antagonist (IL1RN), and interleukin-6 (IL6) genes were analyzed. Results: Castration decreased (p<0.01) the average daily gain and gain/feed ratio. Castration reduced the time spent eating (p<0.001) and the eating frequency (p<0.01) and increased (p<0.001) the lying frequency. Castration temporarily increased (p<0.05) circulating PC and SC concentrations at 0.5 h after castration. Castration temporarily increased (p<0.05) plasma substance P concentrations at 1 d after castration. Castration increased (p<0.05) plasma haptoglobin concentrations at 1 and 3 d after castration. Castration increased (p<0.05) leukocyte mRNA levels of the IL1A, IL1B, IL1RN, and IL6 genes at 6 h after castration. Conclusion: Castration temporarily induced stress and expression of leucocyte inflammatory cytokine genes in Korean cattle bull calves.

Keywords

Acknowledgement

This research was financially supported by IPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries) through the Advanced Production Technology Development Program (grant No: 118016-03, Ministry of Agriculture, Food and Rural Affairs), and by a Korea Research Fellowship (KRF) Program (grant No: 2019H1D3A1A01102738) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT.

References

  1. Welfare implications of castration of cattle [Internet]. American Veterinary Medical Association-Animal Welfare Division. 2014 [cited 2022 Sept 14]. Available from https://www.avma.org/KB/Resources/LiteratureReviews/Pages/castration-cattlebgnd.aspx
  2. Park SJ, Beak SH, Kim SY, et al. Genetic, management, and nutritional factors affecting intramuscular fat deposition in beef cattle-A review. Asian-Australas J Anim Sci 2018;31:1043-61. https://doi.org/10.5713/ajas.18.0310
  3. Park SJ, Piao M, Kim H, et al. Effects of castration and a lidocaine-plus-flunixin treatment on growth and indicators of pain, inflammation, and liver function in Korean cattle bull calves. Livest Sci 2018;216:61-6. https://doi.org/10.1016/j.livsci.2018.07.010
  4. Sutherland MA, Ballou MA, Davis BL, Brooks TA. Effect of castration and dehorning singularly or combined on the behavior and physiology of Holstein calves. J Anim Sci 2013;91:935-42. https://doi.org/10.2527/jas.2012-5190
  5. Gonzalez LA, Schwartzkopf-Genswein KS, Caulkett NA, et al. Pain mitigation after band castration of beef calves and its effects on performance, behavior, Escherichia coli, and salivary cortisol. J Anim Sci 2010;88:802-10. https://doi.org/10.2527/jas.2008-1752
  6. Earley B, Crowe MA. Effects of ketoprofen alone or in combination with local anesthesia during the castration of bull calves on plasma cortisol, immunological, and inflammatory responses. J Anim Sci 2002;80:1044-52. https://doi.org/10.2527/2002.8041044x
  7. Warnock TM, Thrift TA, Irsik M, et al. Effect of castration technique on beef calf performance, feed efficiency, and inflammatory response. J Anim Sci 2012;90:2345-52. https://doi.org/10.2527/jas.2011-4511
  8. Beutler B, Cerami A. Cachectin/tumor necrosis factor: an endogenous mediator of shock and inflammation. Immunol Res 1986;5:281-93. https://doi.org/10.1007/BF02935501
  9. Horwitz W, Latimer GW. AOAC International. Official methods of analysis of AOAC International. 18th ed. Gaithersburg, MD, USA: AOAC International; 2005.
  10. Van Soest PV, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 1991;74:3583-97. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  11. Hernandez CE, Thierfelder T, Svennersten-Sjaunja K, Berg C, Orihuela A, Lidfors L. Time lag between peak concentrations of plasma and salivary cortisol following a stressful procedure in dairy cattle. Acta Vet Scand 2014;56:61. https://doi.org/10.1186/s13028-014-0061-3
  12. O'Loughlin A, McGee M, Waters SM, Doyle S, Earley B. Examination of the bovine leukocyte environment using immunogenetic biomarkers to assess immunocompetence following exposure to weaning stress. BMC Vet Res 2011;7:45. https://doi.org/10.1186/1746-6148-7-45
  13. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. methods. 2001;25:402-8. https://doi.org/10.1006/meth.2001.1262
  14. McKay LI, Cidlowski JA. Pharmacokinetics of corticosteroids. In: Kufe DW, Pollock RE, Weichselbaum RR, et al, editors. Holland-Frei cancer medicine. (6th ed.). Hamilton, ON, Canada: BC Decker; 2003. Available from: https://www.ncbi.nlm.nih.gov/books/NBK13300/
  15. Melendez DM, Marti S, Pajor EA, et al. Effect of band and knife castration of beef calves on welfare indicators of pain at three relevant industry ages: I. Acute pain. J Anim Sci 2017;95:4352-66. https://doi.org/10.2527/jas2017.1762
  16. Schwinn AC, Knight CH, Bruckmaier RM, Gross JJ. Suitability of saliva cortisol as a biomarker for hypothalamic-pituitary-adrenal axis activation assessment, effects of feeding actions, and immunostimulatory challenges in dairy cows. J Anim Sci 2016;94:2357-65. https://doi.org/10.2527/jas.2015-0260
  17. Vining RF, McGinley RA. The measurement of hormones in saliva: possibilities and pitfalls. J Steroid Biochem 1987;27:81-94. https://doi.org/10.1016/0022-4731(87)90297-4
  18. Coetzee JF, Lubbers BV, Toerber SE, et al. Plasma concentrations of substance P and cortisol in beef calves after castration or simulated castration. Am J Vet Res 2008;69:751-62. https://doi.org/10.2460/ajvr.69.6.751
  19. Mintline EM, Varga A, Banuelos J, et al. Healing of surgical castration wounds: a description and an evaluation of flunixin. J Anim Sci 2014;92:5659-65. https://doi.org/10.2527/jas.2014-7885
  20. Murata H, Shimada N, Yoshioka M. Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J 2004;168:28-40. https://doi.org/10.1016/S1090-0233(03)00119-9
  21. Jain S, Gautam V, Naseem S. Acute-phase proteins: as diagnostic tool. J Pharm Bioallied Sci 2011;3:118. https://doi.org/10.4103/0975-7406.76489
  22. Horadagoda NU, Knox KM, Gibbs HA, et al. Acute phase proteins in cattle: discrimination between acute and chronic inflammation. Vet Rec 1999;144:437-41. https://doi.org/10.1136/vr.144.16.437
  23. Brown AC, Powell JG, Kegley EB, et al. Effect of castration timing and oral meloxicam administration on growth performance, inflammation, behavior, and carcass quality of beef calves. J Anim Sci 2015;93:2460-70. https://doi.org/10.2527/jas.2014-8695
  24. Di Paolo NC, Shayakhmetov DM. Interleukin 1α and the inflammatory process. Nat Immunol 2016;17:906-13. https://doi.org/10.1038/ni.3503
  25. Pang WY, Earley B, Murray M, Sweeney T, Gath V, Crowe MA. Banding or Burdizzo castration and carprofen administration on peripheral leukocyte inflammatory cytokine transcripts. Res Vet Sci 2011;90:127-32. https://doi.org/10.1016/j.rvsc.2010.04.023
  26. Schindler R, Mancilla J, Endres S, Ghorbani R, Clark SC, Dinarello CA. Correlations and interactions in the production of interleukin-6 (IL-6), IL-1, and tumor necrosis factor (TNF) in human blood mononuclear cells: IL-6 suppresses IL-1 and TNF. Bood 1990;75:40-7. https://doi.org/10.1182/blood.V75.1.40.40
  27. Arend WP, Gabay C. Physiologic role of interleukin-1 receptor antagonist. Arthritis Res Ther 2000;2:245. https://doi.org/0.1186/ar94
  28. Arend WP, Malyak M, Guthridge CJ, Gabay C. Interleukin-1 receptor antagonist: role in biology. Annu Rev Immunol 1998; 16:27-55. https://doi.org/10.1146/annurev.immunol.16.1.27
  29. Steensberg A, Fischer CP, Keller C, Moller K, Pedersen BK. IL-6 enhances plasma IL-1ra, IL-10, and cortisol in humans. Am J Physiol Endocrinol Metab 2003;285:E433-7. https://doi.org/10.1152/ajpendo.00074.2003
  30. Nicklin MJ, Hughes DE, Barton JL, Ure JM, Duff GW. Arterial inflammation in mice lacking the interleukin 1 receptor antagonist gene. J Exp Med 2000;191:303-12. https://doi.org/10.1084/jem.191.2.303