• 제목/요약/키워드: Stress Concentration factor

검색결과 369건 처리시간 0.029초

Suppressive effects of $Schizandra$ $chinensis$ Baillon water extract on allergy-related cytokine generation and degranulation in IgE-antigen complex-stimulated RBL-2H3 cells

  • Chung, Mi-Ja;Kim, Jeong-Mi;Lee, Sang-Chul;Kim, Tae-Woo;Kim, Dae-Jung;Baek, Jong-Mi;Kim, Tae-Hyuk;Lee, Jae-Sung;Kim, Kyoung-Kon;Yoon, Jin-A;Choe, Myeon
    • Nutrition Research and Practice
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    • 제6권2호
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    • pp.97-105
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    • 2012
  • $Schizandra$ $chinensis$ Baillon is a traditional folk medicine plant that is used to treat and prevent several inflammatory diseases and cancer in Korea, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. This study was designed to investigate mechanisms of anti-allergic activity of a $Schizandra$ $chinensis$ Baillon water extract (SCWE) in immunoglobulin E (IgE)-antigen complex-stimulated RBL2H3 cells and to assess whether gastric and intestinal digestion affects the anti-allergic properties of SCWE. Oxidative stress is an important consequence of the allergic inflammatory response. The antioxidant activities of SCWE increased in a concentration-dependent manner. RBL-2H3 cells were sensitized with monoclonal anti-dinitrophenol (DNP) specific IgE, treated with SCWE, and challenged with the antigen DNP-human serum albumin. SCWE inhibited ${\beta}$-hexosaminidase release and expression of interleukin (IL)-4, IL-13, and tumor necrosis factor-alpha (TNF-${\alpha}$) mRNA and protein in IgE-antigen complex-stimulated RBL2H3 cells. We found that digested SCWE fully maintained its antioxidant activity and anti-allergic activity against the IgE-antigen complex-induced activation of RBL-2H3 cells. SCWE may be useful for preventing allergic diseases, such as asthma. Thus, SCWE could be used as a natural functional ingredient for allergic diseases in the food and/or pharmaceutical industries.

혈액투석환자에서 영양상태와 필수아미노산 섭취의 관련성 연구 (Association of the Nutritional Status and Essential Amino Acids Intake in Hemodialysis Patients)

  • 김혜진;김수안;손정민
    • Journal of Nutrition and Health
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    • 제39권7호
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    • pp.617-623
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    • 2006
  • The mortality and morbidity rate of hemodialysis patients (HD) remain high. Among many factors, protein and calorie malnutrition has been shown to be a major risk factor for increased mortality in the HD patients population. Malnutrition can be caused by insufficient amino acid intake, nutrient losses in dialysate, oxidant stress and muscle catabolism. In this study, we evaluated the association of markers of nutritional status and essential amino acids intake in HD patients. We investigated nutritional status of 41 HD patients (mean age: $64.2\;{\pm}\;11.5\;y$, men: 24, women: 27) by measuring anthropometric, biochemical parameters and food intakes by using 24 hr recall methods. Subject's total energy intake and total protein intake were $1,648.0\;{\pm}\;397.31\;kcal/day,\;79.2\;{\pm}\;27.2\;g/day$:, respectively. The animal protein intake was $42.7\;{\pm}\;22.1\;g/day$, essential amino acids intake was $23.4\;{\pm}\;9.92\;g/day$, and the ratio of essential amino acids to total protein intake was $29.6\;{\pm}\;5.42%$. There were significantly positive correlation between muscle mass and lean body mass with serum creatinine level (r=0.435, p<0.01; r=0.435, p<0,01). There were also significant positive correlation in muscle mass and lean body mass with pre hemodialysis blood urea nitrogen (preHD BUN) (r=0.329, p<0.05; r=0.329, p<0.05). There were no significant correlation in total energy intake and total protein intake per kg ideal body weight (IBW) to muscle mass and lean body mass. However, there were significantly positive correlation between the ratio of essential amino acids and muscle mass and lean body mass (r=0.368, p<0.05; r=0.405, p<0.01). And serum hematocrit concentration was positively correlated with the ratio of essential amino acids (r=0.032, p<0.05). The results of this study indicate that strong associations exist in essential amino acid intakes with malnutrition than total protein intakes in HD patient. In conclusion, specialized nutrition education should be necessary to efficiently improve the quality of protein intakes.

p-수렴 완전층별모델에 의한 일면패치로 보강된 원공 적층판의 휨효과 (Bending Effect of Laminated Plates with a Circular Hole Repaired by Single-Sided Patch Based on p-Convergent Full Layerwise Model)

  • 우광성;양승호;안재석;신영식
    • 한국전산구조공학회논문집
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    • 제22권5호
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    • pp.463-474
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    • 2009
  • 기존에 설치되어 있는 구조물의 양면대칭 패치보강은 항상 면내거동만을 유발하나 시공상 어려움이 있다. 반면에 일면 패치보강의 경우 인장력의 증가에 따라 중립축의 위치가 대칭이 아니므로 휨에 대한 강성도가 증가하게 되며, 결과적으로 적층판의 휨을 심화시키게 된다. 이 연구에서는 일면 패치보강된 적층판의 두께방향은 물론이고 원공주위의 응력집중계수를 산정하기 위해 p-수렴 완전층별모델을 제안하였다. 가정된 변위장의 정의를 위해, 임의의 층에서 변위-변형률 관계와 3차원 구성방정식은 2차원 및 3차원 계층적 형상함수의 조합이 사용된다. 원형경계의 기하형상을 나타내기 위해 초유한사상기법이 사용되며, 다른 외삽법을 사용하지 않고 각 층마다 절점에서의 응력값을 직접적으로 얻기위해 가우스-로바토 수치 적분이 수행되었다. 제안된 모델의 정확도와 단순성은 기존의 3차원 유한요소해석과 실험에 의해 구해진 결과들과의 비교를 통해 검증되었다. 또한 정사각형, 원형, 고리형 형상의 다양한 패치보강에 따른 휨효과를 조사하였다.

Significance of $p27^{kip1}$ as potential biomarker for intracellular oxidative status

  • Quintos, Lesley;Lee, In-Ae;Kim, Hyo-Jung;Lim, Ji-Sun;Park, Ji-A;Sung, Mi-Kyung;Seo, Young-Rok;Kim, Jong-Sang
    • Nutrition Research and Practice
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    • 제4권5호
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    • pp.351-355
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    • 2010
  • Our previous proteomic study demonstrated that oxidative stress and antioxidant delphinidin regulated the cellular level of $p27^{kip1}$ (referred to as p27) as well as some heat shock proteins in human colon cancer HT 29 cells. Current study was conducted to validate and confirm the regulation of these proteins using both in vitro and in vivo systems. The level of p27 was decreased by hydrogen peroxide in a dose-dependent manner in human colon carcinoma HCT 116 (p53-positive) cells while it was increased upon exposure to hydrogen peroxide in HT 29 (p53-negative) cells. However, high concentration of hydrogen peroxide (100 ${\mu}M)$ downregulated p27 in both cell lines, but delphindin, one of antioxidative anthocyanins, enhanced the level of p27 suppressed by 100 ${\mu}M$ hydrogen peroxide. ICR mice were injected with varying concentrations of hydrogen peroxide, delphinidin and both. Western blot analysis for the mouse large intestinal tissue showed that the expression of p27 was upregulated by 25 mg/kg BW hydrogen peroxide. To investigate the association of p27 regulation with hypoxia-inducible factor 1-beta (HIF-$1{\beta}$), the level of p27 was analyzed in wild-type mouse hepatoma hepa1c1c7 and Aryl Hydrocarbon Nuclear Translocator (arnt, HIF-$1{\beta}$)-defective mutant BPRc1 cells in the absence and presence of hydrogen peroxide and delphinidin. While the level of p27 was responsive to hydrogen peroxide and delphinidin, it remained unchanged in BPRc1, suggesting that the regulation of p27 requires functional HIF-$1{\beta}$. We also found that hydrogen peroxide and delphinidin affected PI3K/Akt/mTOR signaling pathway which is one of upstream regulators of HIFs. In conclusion, hydrogen peroxide and antioxidant delphinidin seem to regulate intracellular level of p27 through regulating HIF-1 level which is, in turn, governed by its upstream regulators comprising of PI3K/Akt/mTOR signaling pathway. The results should also encourage further study for the potential of p27 as a biomarker for intracellular oxidative or antioxidant status.

Spermatogenic and Antioxidant Potential of Mucuna prureins (L.) in Epididymal Spermatozoa: A Dose Dependent Effect

  • Suresh, Sekar;Prithiviraj, Ealumali;Venkatalakshmi, Nagella;Ganesh, Mohanraj Karthik;Ganesh, Lakshmanan;Lee, Hyun-Jeong;Prakash, Seppan
    • Reproductive and Developmental Biology
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    • 제35권4호
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    • pp.441-447
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    • 2011
  • The study aim is to investigate the free radicals scavenging and spermatogenic potentials, as well as to analyze any reproductive toxicity of ethanolic extract of Mucuna prureins (M. pruriens) Linn. in spermatozoa, under different dosages in normal male rat. Normal rats were randomly selected and suspension of the extract was administered orally at the dosages of 150, 200 and 250 mg/kg body weight of the different groups of male rats (n=6) once in a day for 60 days and grouped as group II, III and IV respectively. Saline treated rats served as control -group I. On the $60^{th}$ day the animals were sacrificed and the epididymal sperm were subjected to various analyses like level of ROS production, LPO, enzymatic and non enzymatic antioxidant, morphology, morphometry, chromosomal integrity and DNA damage. Results showed significant reduction in ROS production and peroxidation and significant increase in both enzymic and non-enzymic antioxidants in all concentration treated groups when compared with control. Results from all the drug treated groups showed good sperm morphology, increased sperm count and motility. There was no DNA damage and showed normal chromosomal integrity even in 250 mg/kg dose. When compared with control all the three extract treated groups showed increased ROS scavenging activity. However, group II (200 mg/kg) showed significant changes in all the parameters. From the present study it was confirmed that the M. pruriens has potential to improve the sperm qualitatively and quantitatively through scavenging the excess ROS with any adverse side effects. These observations suggest that ethanolic seed extract of M. pruriens may serve as anti-oxidant that can exploit to treat the oxidative stress mediated male factor infertility.

흰쥐의 합곡부위(合谷部位)에 황련약침(黃連藥鍼)이 TNBS로 유발된 대장염(大腸炎)에 미치는 영향(影響) (Effects of Rhizoma Coptidis Herbal Acupuncture applied to HapGok $(LI_4)$ on TNBS-induced Colitis in rats)

  • 도진우;안성훈;구성태;백대봉;김경식;손인철
    • Korean Journal of Acupuncture
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    • 제21권2호
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    • pp.95-110
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    • 2004
  • Objectives : Ulcerative colitis and Crohn's diseases are chronic inflammatory disease of the gastrointestinal tract identified mainly for diarrhea, stomachache, hemafecia. The source and pathologic mechanism about ulcerative colitis and Crohn's disease are still unknown but stress, infection, a genetic factor, environmental factors etc. may be the cause of chronic inflammatory disease of the gastrointestinal tract recently. Methods : Because ulcerative colitis and Crohn's disease is recognized as Ha-ri(下痢) or Jang-Byok(腸?) in Oriental Medicine, Rhizoma Coptidis Herbal acupunctures were injected in hapgok $(LI_4)$ which is used to treat intestinal disease in clinic. All animals were subjected to the injection of saline $(300\;{\mu}l,\;500\;{\mu}l)$ for a study control and TNBS $(300\;{\mu}l,\;500\;{\mu}l)$ into the lumen of the colon, 8cm proximal to the anus through the intestine. Rhizoma Coptidis Herbal (20 mg/ml, 0.4 ml) acupuncture solution was injected to the $LI_4$ (both hands) at the secondary injection time of TNBS in rats. And body weight, RBC count, WBC count, total protein, Paw edema rate, rate of protein leakage into CMC-pouch fluid, IgG levels and IgM levels were observed to study the effects of Rhizoma Coptidis Herbal acupuncture in hapgok $(LI_4)$. Results : In results, Rhizoma Coptidis Herbal acupuncture in hapgok $(LI_4)$ on TNBS-induced colitis reduced the rate of weight loss but had no effect on RBC and WBC count. And it reduced total protein concentration, paw edema, rate of protein leakage into CMC-pouch fluid, IgG levels and IgM levels. Conclusion : Data suggest that injection of Rhizoma Coptidis herbal acupuncture solution into hapgok has significant therapeutic effect on the colitis.

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합곡에 대한 애엽 약침이 흰쥐 대장염 완화에 미치는 효과 (Artemisiae argyi herbal acupuncture of Hapgok (LI4) ameliorates TNBS-induced colitis in rats)

  • 변기원;임용수;구성태;김재효;김경식;손인철
    • Korean Journal of Acupuncture
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    • 제24권1호
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    • pp.79-94
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    • 2007
  • Objectives : Ulcerative colitis and Crohn's disease are chronic inflammatory disease of the gastrointestinal tract was identified mainly for diarrhea, stomachache, hemafecia. The source and pathologic mechanism about ulcerative colitis and crohn's disease are still unknown but stress, infection, a genetic factor, environmental factors etc. may be the cause of chronic inflammatory disease of the gastrointestinal tract recently. The purpose of the present study is to evaluate the effects of herbal acupuncture with Artemisiae Argyi for the treatment to intestinal disease in the rat with 2, 4, 6 - trinitrobenzenesulfonic acid (TNBS) induced colitis. Methods : Artemisiae Argyi Herbal acupunctures were infected in HapGok (LI4) which is used to treat for intestinal disease in clinic. All animals were subjected to the injection of saline (300 ${\mu}l$, 500 ${\mu}l$) for a study control and TNBS (300 ${\mu}l$, 500 ${\mu}l$) into the lumen of the colon, 8cm proximal to the anus through the intestine. Artemisiae Agryi' Herbal (20 mg/ml, 0.4 ml) acupunctures were injected to the LI4 (both hands) at the secondary injection time of TNBS in rats. And body weight, RBC count, WBC count, total protein, Paw edema rate, rate of protein leakage into CMC-pouch fluid, IgG levels and IgM levels were observed to study the effects of Artemisiae Argyi' Herbal acupuncture in HapGok (Ll4). Results : Artemisiae Argyi' Herbal acupuncture in HapGok (Ll4) on TNBS-induced colitis inhibit the body weight lose rate but not RBC and WBC count. Furthermore, it inhibited the reduction of total protein concentration, paw edema, rate of protein leakage into CMC-pouch fluid, IgG levels and IgM levels. Conclusions : Herbal acupuncture with Artemisiae Argyi helps recover the TNBS-induced colonic damage and may be an important method for treatment of the colitis.

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A Study on Stability Evaluation of the Nail-Anchor Mixed Support System

  • Kim, Hong-Taek;Cho, Yong-Kwon;Yoo, Han-Kyu
    • 한국지반공학회논문집
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    • 제15권3호
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    • pp.41-70
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    • 1999
  • 쏘일네일 또는 지반앵커와 같은 보강재는 지하굴착 및 사면의 안정성 확보를 위해 효율적으로 적용될 수 있음이 여러 지반공학자들에게 널리 알려져 있다. 그러나 경우에 따라서는, 쏘일네일과 지반앵커를 굴착면 상하 또는 좌우에 복합적으로 적용하여 지반굴착이 진행되는 시공사례가 종종 있어 왔다. 본 연구에서는 한계평형적 접근에 근거하여, 이와같은 상하 또는 좌우 쏘일네일-지반앵커 복합 지지시스템의 전체적인 안정성을 평가할 수 있는 해석법을 제시하였다. 이 과정에서 예상 파괴흙쐐기의 형상은 $FLAC^{2D}\; 및\; FLAC^{3D}$ 프로그램 해석결과를 토대로 결정하였다. 또한 관입전단파괴에 대한 안정성 확보를 위해 요구되는 본 쏘일네일-지반앵커 복합 지지시스템의 전면부 숏크리트 벽체의 두께를 검토하였다. 아울러 쏘일네일 구조체와 지반앵커 구조체가 서로 접하게 되는 경계영역에서는 응력집중, 상대변위 및 이로 인한 전단력 유발 등 전면벽체에 대한 추가 안정성 검토가 요구된다. 이를 위해, 경계영역에서의 상대변위를 예측키 위한 간편 유한요소해석기법을 제시하였으며, 또한 충분한 안정성 확보를 위해 숏크리트 전면벽체의 두께가 과도하게 요구되는 문제점을 적절히 해결하기 위해 수정된 지압판 시스템을 제시하였다. 아울러 관련 설계변수들의 영향에 대한 다양한 분석과 더불어, 예측된 상대변위를 $FLAC^{2D}$프로그램 해석결과와 서로 비교하여 제시된 간편 유한요소해석기법의 적용 가능성을 평가하였다.

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파프리카 생산성 차이 농가 간 지상부 환경요인 비교 (Comparison of Atmospheric Environmental Factors between Farms with Difference in Paprika Productivity)

  • 김가영;우승미;김호철
    • 문화기술의 융합
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    • 제7권4호
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    • pp.785-789
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    • 2021
  • 파프리카 생산성은 동일한 품질 온실, 지역 간에 차이를 나타내고 있다. 이러한 차이는 다양한 환경요인의 차이에서 오는 것으로 알려져 있다. 본 연구는 동일 지역에서 동일 품질 온실 중에서 생산성이 높거나(HPF) 낮은(LPF) 온실 간 내부 환경요인의 수준 차이를 알아보고자 수행되었다. 주요 환경요인들의 일 또는 주의 평균값에서 두 온실 간에 가장 큰 차이를 보인 것은 CO2 농도였지만, LPF에서 HPF보다 매우 높아 생산성 차이 요인으로 판정하기 않았다. 14개 환경 요인들 간 상관분석에서 광량과 수분 관련 요인들 간에 상관성을 나타내었다. 증산량에 관련된 수증기압포차와 상대습도 간 선형회귀모형의 회귀계수는 HPF에서 -0.0202kpa, LPF에서는 -0.0262kpa로 나타났다. 특히 2월과 3월에는 LPF에서 VPD가 1.5kpa 이상이었고, 재배 초기에 누적 광량 대비 누적 수증기압포차의 급격한 증가가 나타났다. 따라서 LPF에서 생산성이 낮은 원인은 재배 초기와 겨울철에 적은 광량 조건에서 높은 VPD에 의한 높은 증산량이 식물체에게 수분 스트레스를 주었기 때문으로 판단된다.

Altitude training as a powerful corrective intervention in correctin insulin resistance

  • Chen, Shu-Man;Kuo, Chia-Hua
    • 운동영양학회지
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    • 제16권2호
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    • pp.65-71
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    • 2012
  • Oxygen is the final acceptor of electron transport from fat and carbohydrate oxidation, which is the rate-limiting factor for cellular ATP production. Under altitude hypoxia condition, energy reliance on anaerobic glycolysis increases to compensate for the shortfall caused by reduced fatty acid oxidation [1]. Therefore, training at altitude is expected to strongly influence the human metabolic system, and has the potential to be designed as a non-pharmacological or recreational intervention regimen for correcting diabetes or related metabolic problems. However, most people cannot accommodate high altitude exposure above 4500 M due to acute mountain sickness (AMS) and insulin resistance corresponding to a increased levels of the stress hormones cortisol and catecholamine [2]. Thus, less stringent conditions were evaluated to determine whether glucose tolerance and insulin sensitivity could be improved by moderate altitude exposure (below 4000 M). In 2003, we and another group in Austria reported that short-term moderate altitude exposure plus endurance-related physical activity significantly improves glucose tolerance (not fasting glucose) in humans [3,4], which is associated with the improvement in the whole-body insulin sensitivity [5]. With daily hiking at an altitude of approximately 4000 M, glucose tolerance can still be improved but fasting glucose was slightly elevated. Individuals vary widely in their response to altitude challenge. In particular, the improvement in glucose tolerance and insulin sensitivity by prolonged altitude hiking activity is not apparent in those individuals with low baseline DHEA-S concentration [6]. In addition, hematopoietic adaptation against altitude hypoxia can also be impaired in individuals with low DHEA-S. In short-lived mammals like rodents, the DHEA-S level is barely detectable since their adrenal cortex does not appear to produce this steroid [7]. In this model, exercise training recovery under prolonged hypoxia exposure (14-15% oxygen, 8 h per day for 6 weeks) can still improve insulin sensitivity, secondary to an effective suppression of adiposity [8]. Genetically obese rats exhibit hyperinsulinemia (sign of insulin resistance) with up-regulated baseline levels of AMP-activated protein kinase and AS160 phosphorylation in skeletal muscle compared to lean rats. After prolonged hypoxia training, this abnormality can be reversed concomitant with an approximately 50% increase in GLUT4 protein expression. Additionally, prolonged moderate hypoxia training results in decreased diffusion distance of muscle fiber (reduced cross-sectional area) without affecting muscle weight. In humans, moderate hypoxia increases postprandial blood distribution towards skeletal muscle during a training recovery. This physiological response plays a role in the redistribution of fuel storage among important energy storage sites and may explain its potent effect on changing body composition. Conclusion: Prolonged moderate altitude hypoxia (rangingfrom 1700 to 2400 M), but not acute high attitude hypoxia (above 4000 M), can effectively improve insulin sensitivity and glucose tolerance for humans and antagonizes the obese phenotype in animals with a genetic defect. In humans, the magnitude of the improvementvaries widely and correlates with baseline plasma DHEA-S levels. Compared to training at sea-level, training at altitude effectively decreases fat mass in parallel with increased muscle mass. This change may be associated with increased perfusion of insulin and fuel towards skeletal muscle that favors muscle competing postprandial fuel in circulation against adipose tissues.