• 농업과학연구
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• 제47권3호
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• pp.597-604
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• 2020

Exposure to heat stress (HS) has negative effects on pig production and health. Plasma concentrations of amino acids (AAs) can be used as indicators of HS. Therefore, this study was conducted to evaluate the effect of acute HS on feed intake, water drink, and plasma AAs in pigs. A total of 6 growing pigs (n = 6, 3 boars and 3 gilts) were raised in thermal neutral (TN; 25℃) conditions for the 5-d adaptation period as a control. After the adaptation, pigs were exposed to HS at 33℃ (HS33) for 24 h. All the pigs were fed the same diet formulated to meet or exceed predicted requirements during the whole experimental period. Blood samples were collected after the adaptation and heat treatment to verify the AAs. Measurements were the average daily feed intake (ADFI), average daily water intake (ADWI), water loss, tumor necrosis factor (TNF)-α, nitrate, total nitric oxide, and AAs in the plasma samples. Data were analyzed using the PROC GLM of SAS. HS33 had a lower ADFI (p < 0.05) and a tendency for an increased ADWI and water loss compared to TN. The level of TNF-α was significantly decreased (p < 0.05) in HS33. HS33 had a lower concentration of histidine and sarcosine compared to TN; however, there were no differences in the levels of nitrates and total nitric oxide. In conclusion, the results of this study show the differential effect of HS on the plasma concentration of AAs. We expect that the changed AAs could be potential indicators of HS.

• Asian-Australasian Journal of Animal Sciences
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• 제27권7호
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• pp.1035-1043
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• 2014

To investigate the effect of dietary Acanthopanax senticosus polysaccharide (ASPS) on growth performance, immunity, blood parameters and mRNA expression of pro-inflammatory cytokines in immunologically challenged piglets, an experiment employing $2{\times}2$ factorial arrangement concerning dietary ASPS treatment (0 or 800 mg/kg) and immunological challenge (lipopolysaccharide [LPS] or saline injection) was conducted with 64 crossbred piglets (weaned at 28 d of age, average initial body weight of $7.25{\pm}0.21kg$) assigned to two dietary ASPS treatments with 8 replicates of 4 pigs each. Half of the piglets of per dietary treatment were injected with LPS or saline on d 14. Blood samples were obtained at 3 h after immunological injection on d 14 and piglets were slaughtered to obtain spleen samples on d 21. Dietary ASPS did not affect average daily gain (ADG) (p = 0.634), average daily feed intake (ADFI) (p = 0.655), and gain:feed (p = 0.814) prior to LPS challenge. After LPS challenge, for LPS-challenged pigs those fed ASPS had higher ADG and ADFI than the non-supplemented group (p<0.05), and an interaction between $LPS{\times}ASPS$ was observed on the two indices (p<0.05). Dietary ASPS improved lymphocyte proliferation among saline-injected and LPS-injected pigs (p<0.05). Interaction between $LPS{\times}ASPS$ was also revealed on lymphocyte proliferation (p<0.05). Circulatory concentration of IgG was influenced neither by ASPS (p = 0.803) or LPS (p = 0.692), nor their interaction (p = 0.289). Plasma concentration and spleen mRNA expression of interleukin-1beta (IL-$1{\beta}$), interleukin-6 (IL-6), and tumor necrosis factor (TNF)-${\alpha}$ were induced to increase (p<0.05) by LPS challenge, in contrast, these indices were decreased by dietary ASPS (p<0.05), and interactions were found on these cytokines (p<0.05). For LPS-challenged pigs, dietary ASPS also reduced the circulating concentration and spleen mRNA expression of IL-$1{\beta}$, IL-6 as well as TNF-${\alpha}$ (p<0.05). The interaction between $LPS{\times}ASPS$ was also observed on the circulating concentration of insulin-like growth factor-I, ${\alpha}$-acid glycoprotein (${\alpha}$-AGP), nonesterified fatty acid, and glucose (p<0.05). The results of this study demonstrate that dietary ASPS can modulate the release of pro-inflammatory cytokines during immunological challenge, which might enable piglets to achieve better growth performance.

• 대한화장품학회지
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• 제46권3호
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• pp.253-263
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• 2020

활성산소종(reactive oxygen species, ROS)은 우리 몸의 항상성 유지에 있어 중요한 역할을 한다. 그러나 과도한 ROS의 생성은 단백질, 지질, 핵산과 같은 세포 구성성분을 손상시키고 피부노화를 촉진시킨다. 이에 본 연구에서는 과도한 산화 스트레스를 예방하기 위해 Chungsimyeonja-um (CSYJE)의 항산화 효과를 확인하였다. 먼저 DPPH 및 ABTS assay를 실시하여 CSYJE의 항산화 효과를 확인한 결과 농도 의존적으로 radical 소거 활성을 확인하였다. 세포생존율 확인을 위해 MTT assay를 실시한 결과 1,000 ㎍/mL 농도에서 세포 독성이 없음을 확인하였다. 항산화 관련 단백질인 nuclear-E2-related factor 2 (Nrf2), Heme oxygenase-1 (HO-1)의 발현 수준을 확인하기 위해 western blotting을 실시한 결과 농도 의존적으로 발현이 증가하는 것을 확인하였다. 세포 내 ROS유발 물질인 lipopolysaccharide (LPS)로 ROS를 유도한 후, ROS생성 억제효과를 확인하기 위해 DCF-DA 염색법을 실시한 결과 농도 의존적으로 ROS 생성 억제효과를 확인하였으며 ROS의 생성으로 인한 염증성 사이토카인과 염증인자의 mRNA발현 수준을 확인하기 위해 real-time RT-PCR을 실시한 결과 농도 의존적으로 염증성 사이토카인과 염증인자의 mRNA 발현을 억제시켰다. 따라서, 본 연구는 Nrf2/HO-1 신호 전달 경로 활성을 통해 CSYJE의 항산화효과를 확인했으며 이는 CSYJE가 활성산소를 억제하여 항산화 화장품의 재료로서 사용될 수 있음을 시사한다.

• Environmental Analysis Health and Toxicology
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• 제29권
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• pp.10.1-10.6
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• 2014

Objectives Cigarette smoking had been recorded as the main cause of impaired endothelium-dependent vasodilation in smokers by reducing nitric oxide (NO), a production of endothelial nitric oxide synthase (eNOS). However, the mechanism of NO impairment via eNOS activity is unclear until now. In this study, cell passage is suggested to be a relevant factor to eNOS expression under cigarette smoking stress. Methods Bovine aortic endothelial cells (BAECs) were chosen as the research subject with passages ranking from 6 to 9 (6P to 9P). After exposure of cigarette smoking extract (CSE) solution, MTT assay and Western blot method were performed to check the cell viability as well as eNOS protein concentration. In these experiments, four concentrations of CSE at 0.5, 1, 2, and 4% were selected for treatment. Results Our results showed that cells almost died at 4% of CSE. Besides, eNOS protein mass had a linear decrease under the increase of CSE concentration. In addition, the effect of CSE on eNOS expression was dissimilar between different passages. Conclusions This study indicated that CSE had effect on both cell viability and eNOS expression. Besides, a reduction in protein mass was matched with the decrease of cell viability due to CSE tress. Last but not least, the response of eNOS protein to different concentration of CSE at different passages was disparate, making the hypothesis about cell passage related inhibition of eNOS caused by CSE solution.

• Fisheries and Aquatic Sciences
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• 제19권10호
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• pp.44.1-44.8
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• 2016

Rockfish, Sebastes schlegelii (mean length $14.53{\pm}1.14cm$ and mean weight $38.36{\pm}3.45g$), were exposed for 4 weeks with the different levels of ammonia in the concentrations of 0, 0.1, 0.5, and 1.0 mg/L at 19 and $24^{\circ}C$. The indicators of growth performance such as daily length gain, daily weight gain, condition factor, and hematosomatic index were significantly reduced by the ammonia exposure and high temperature. The ammonia exposure induced a significant decrease in hematological parameters, such as red blood cell (RBC) count, white blood cell (WBC) count, hemoglobin (Hb), and hematocrit (Ht), whose trend was more remarkable at $24^{\circ}C$. Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were also notably decreased by the ammonia exposure. Blood ammonia concentration was considerably increased by the ammonia concentration exposure. In the serum components, the glucose, glutamic oxalate transaminase (GOT), and glutamic pyruvate transaminase (GPT) were substantially increased by the ammonia exposure, whereas total protein was significantly decreased. But, the calcium and magnesium were not considerably changed.

• 한국HCI학회:학술대회논문집
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• 한국HCI학회 2008년도 학술대회 1부
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• pp.158-162
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• 2008

심박은 주위에 환경적, 신체적, 정신적 상태가 많은 영향을 끼친다. 실험에서 정서를 판단함에 있어서 안정된 상태를 만들어야 정확한 데이타를 얻을 수 있다. 사람은 하루 중 심박수가 시간 및 상태에 따라서 다르다. 그래서 심박수의 안정 상태를 만들어야 한다. 의학에서는 신체적으로 볼 때 낮아지는 심박수일수록 안정 상태이다. 하지안 사람마다 평균 심박수가 다르고 시간마다 심박수 평균이 달라진다. 그래서 심박수로는 안정 심박을 구하기가 힘들다. 심박 패턴을 추출할 때 장기 분석을 통한 검증이 정확하다. 하지만 장기 분석을 하지 않아도 피험자의 안정 상태를 유도 할 수 있다면 장기 분석을 하지 않아도 된다. 안정 심박 패턴을 유도하기 위해서 사용한 청각자극은 우리가 일반적으로 집중력과 스트레스 피로를 해소하는 MC Square와 모차르트 음악을 선정하였다. 두 개의 청각 자극이 안정된 심박을 유도 와 안정된 심박 패턴을 만들 수 있는지를 분석하여 유사점과 차이점을 비교하였다. 본 논문은 분당 심박수 변화와 심박 패턴분석으로 청각자극이 안정된 심박수를 유도하는 유의미한 결과 얻었으며 주위의 영향을 받지 않는 상태 즉, 평상심을 유도 할 수 있었다.

• 생물정신의학
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• 제13권2호
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• pp.95-102
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• 2006

Object : Nerve growth factor(NGF) is a protein involved in neuronal survival and plasticity in the central nervous system, which might play an important role in stress, depression and suicide. This study was performed to determine whether there is an alteration in plasma NGF concentrations in depressed patients with suicidal attempt. Methods : The subjects were 32 depressed patients who attempted suicide and admitted in emergency room. Forty-four hospitalized non-suicidal depressive patients and the 30 normal controls were closely matched with the suicidal group in terms of age and sex. Individuals in all 3 groups were evaluated independently by a semi-structured interview for the purpose of establishing a DSM-IV criteria diagnosis. The severity of depressive symptoms was evaluated using Hamilton depression rating scale(HDRS). The severity of the suicidal behavior was evaluated by Weisman and Worden's risk-rescue rating(RRR) system and the Lethality Suicide Attempt Rating Scale(LSARS). Plasma NGF level was measured by the enzyme linked immunosorbent assay(ELISA) method. Results : There were no statistically significant differences of the plasma NGF levels among groups. LSARS and RRR did not reveal any significant correlation with ${\beta}$-NGF level in suicidal depressive patients. Conclusion : This study do not support an association between ${\beta}$-NGF and suicidal depression. However it is necessary to investigate this association through other route such as postmortem brain.

• Molecules and Cells
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• 제42권4호
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• pp.292-300
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• 2019

Immunometabolism, defined as the interaction of metabolic pathways with the immune system, influences the pathogenesis of metabolic diseases. Metformin and carbon monoxide (CO) are two pharmacological agents known to ameliorate metabolic disorders. There are notable similarities and differences in the reported effects of metformin and CO on immunometabolism. Metformin, an anti-diabetes drug, has positive effects on metabolism and can exert anti-inflammatory and anti-cancer effects via adenosine monophosphate-activated protein kinase (AMPK)-dependent and AMPK-independent mechanisms. CO, an endogenous product of heme oxygenase-1 (HO-1), can exert anti-inflammatory and antioxidant effects at low concentration. CO can confer cytoprotection in metabolic disorders and cancer via selective activation of the protein kinase R-like endoplasmic reticulum (ER) kinase (PERK) pathway. Both metformin and CO can induce mitochondrial stress to produce a mild elevation of mitochondrial ROS (mtROS) by distinct mechanisms. Metformin inhibits complex I of the mitochondrial electron transport chain (ETC), while CO inhibits ETC complex IV. Both metformin and CO can differentially induce several protein factors, including fibroblast growth factor 21 (FGF21) and sestrin2 (SESN2), which maintain metabolic homeostasis; nuclear factor erythroid 2-related factor 2 (Nrf2), a master regulator of the antioxidant response; and REDD1, which exhibits an anticancer effect. However, metformin and CO regulate these effects via different pathways. Metformin stimulates p53- and AMPK-dependent pathways whereas CO can selectively trigger the PERK-dependent signaling pathway. Although further studies are needed to identify the mechanistic differences between metformin and CO, pharmacological application of these agents may represent useful strategies to ameliorate metabolic diseases associated with altered immunometabolism.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 1999년도 봄 학술발표회 논문집(I)
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• pp.774-780
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• 1999

The study of bond behavior between concrete and rebar has been performed for a long time. On this study, we tried to analysed variation of bond behaviors quantitatively with varying the strength of concrete. Bond stress which observed below the neutral surface of beam and at connecting part of beam and column is affected by various bond parameters. Resistance of deformed bars which embedded in concrete to the pullout force is divided 1) chemical adhesive force 2) frictional force 3) mechanical resistance of ribs to the concrete and these horizontal components of resistance is being bond strength. We selected the most common and typical variable which is concrete strength among various variables. So we used two kinds of concrete strength like as 25MPa(NSC) and 65MPa(HSC). Tension Test was performed to verify how bond behavior varied with two kinds of concrete strength. Concentration of bond stress was observed at load-end commonly in Tension Test of the initial load stage. At this stage stress distribution was almost coincident at each strength. As tension load added, this stress distribution had difference gradually and movement of pick point of bond stress to free-end and central section was observed. This tendency was observed at first and moving speed was more fast in NSC. At the preceeding result the reason of this phenomenon is considered to discretion of chemical adhesion and local failure of concrete around rebar in load-end direction. Especially, when concrete strength was increased 2.6 times in tension test, ultimate bond strength was increased 1.45 times. In most recent used building codes, bond strength is proportioned to sqare root of concrete compressive strength but comparison of normalized ultimate bond strength was considered that the higher concrete strength is, the lower safety factor of bond strength is in each strength if we use existing building codes. In Tension Test, in case of initial tensile force state, steel tensile stress of central cross section is not different greatly at each strength but tensile force increasing, that of central cross section in NSC was increased remarkably. Namely, tensile force which was shared in concrete in HSC was far greater than that of concrete in NSC at central section.

• 구강회복응용과학지
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• 제29권3호
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• pp.259-271
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• 2013

임플란트 고정체-지대주 결합구조체의 형태에 따른 교합부하의 반응이 다양하여 본 연구에서는 하중위치 및 결합구조체 접촉 비율에 따라 3단 계단형 결합구조체와 경사형에서 어떠한 차이가 있는 지를 3차원 유한요소분석을 시행하였다. 2종의 임플란트-지대주 결합 구조체에 연결된 상부 치관을 제작하여 각 치관에 설정된 하중위치에 200 N의 하중을 부여하였다. 임플란트 중심 부위에서 하중조건이 멀어질수록 피질골정에 가해지는 응력이 증가되기에 하중조건은 응력발생에 영향을 미치는 주요 요소이며 다음으로 결합구조형태에도 영향을 받았다. 또한 수직 하중에 비해 빗금 경사 하중이 부여된 경우 계단형은 경사형에 비해 유리한 응력 분포를 보였다. 그리고 지대주 결합구조체가 고정체의 내벽에 대해 접촉이 많아 질수록 골질에 대한 응력분산이 유리한 것으로 나타났다. 결론적으로 고정체 폭경에서 벗어난 빗김 수직 및 경사 하중은 결합구조체의 종류와 관계없이 피질골정에 응력을 집중시키므로 저작기능시 교합접촉면을 고정체의 폭경 내에 위치하도록 하는 것이 생체역학적으로 바람직 할 것으로 사료되었다.