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http://dx.doi.org/10.15230/SCSK.2020.46.3.253

Anti-oxidative Effect of Chungsimyeonja-um (CSYJE) via Nrf2/HO-1 Pathway Activity in Lipopolysaccharide (LPS) Induced RAW 264.7 Macrophages  

Jeon, Seon Hong (Department of Cosmetic Science, Semyung University)
Oh, Sol La (Department of Cosmetic Science, Semyung University)
Kim, So Jeong (Department of Cosmetic Science, Semyung University)
Jeon, Bo Hee (Department of Cosmetic Science, Semyung University)
Sung, Jin Young (Department of Cosmetic Science, Semyung University)
Kim, Yong Min (Department of Cosmetic Science, Semyung University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.46, no.3, 2020 , pp. 253-263 More about this Journal
Abstract
Reactive oxygen species (ROS) plays an important role in maintaining homeostasis. However, excessive ROS production damages cellular components such as proteins, lipids, and nucleic acids and promotes skin aging. In this study, we confirmed the antioxidant effect of CSYJE to prevent excessive oxidative stress. First, DPPH and ABTS assays were performed to confirm the antioxidant effect of CSYJE and the radical scavenging activity was confirmed depending on the concentration. As a result of performing the MTT assay to confirm the cell viability, it was confirmed that there was no cytotoxicity at a concentration of 1,000 ㎍/mL. As a result of western blotting to confirm the expression levels of the antioxidant-related proteins nuclear-E2-related factor 2 (Nrf2) and Heme oxygenase-1 (HO-1), it was confirmed that the expression was increased in a concentration-dependent manner. After inducing ROS with lipopolysaccharide (LPS), an intracellular ROS-causing substance, DCF-DA was performed to confirm the inhibitory effect of ROS production, and the inhibition of ROS production was confirmed to concentration-dependent. Real-time RT-PCR was performed to confirm the mRNA expression level of inflammatory cytokines and inflammatory mediator caused by ROS generation, mRNA expression was reduced in a dose dependent manner. Therefore, this study confirmed the antioxidant effect of CSYJE through the Nrf2/HO-1 signaling pathway, which suggests that CSYJE can be used as an antioxidant cosmetic material by inhibiting free radicals.
Keywords
anti-oxidative; Chungsimyeonja-um (CSYJE); HO-1; Nrf2; RAW 264.7;
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1 K. J. Trouba, H. K. Hamadeh, R. P. Amin, and D. R. Germolec, Oxidative stress and its role in skin disease, Antioxid. Redox Signal., 4(4), 665 (2002).   DOI
2 J. W. Kaspar, S . K. Niture, and A. K. Jaiswal, Nrf2:inrf2 (keap1) signaling in oxidative stress, Free Radical Biology and Medicine, 47(9), 1304 (2009).   DOI
3 J. A. Johnson, D. A. Johnson, A. D. Kraft, M. J. Calkins, R. J. Jakel, M. R. Vargas, and P. C. Chen, The nrf2-are pathway: an indicator and modulator of oxidative stress in neurodegeneration, Annals of The New York Academy of Sciences, 1147(1), 61 (2008).   DOI
4 L. Li, P. Grenard, J. T. Van Nhieu, B. Julien, A. Mallat, A. Habib, and S. Lotersztajn, Heme oxygenase-1 is an antifibrogenic protein in human hepatic myofibrobalsts, Gastroenterology, 125(2), 460 (2003).   DOI
5 A. Nakao, L. E. Otterbein, M. Overhaus, J. K. Sarady, A. Tsung, K. Kimizuka, and F. H. Bach, Biliverdin protects the functional integrity of a transplanted syngeneic small bowel, Gastroenterology, 127(2), 595 (2004).   DOI
6 S. R. Yoo, H. K. Ha, N. R. Lee, H. K. Shin, C. S. Seo, A study on change of marker compounds and biological activity in chungsimyeonja-eum decoction depending on a storage temperature and periods, The Korea Journal of Herbology, 32(4), 25 (2017).   DOI
7 I. J. Rhee and D. M. Lee, The effect of some antipolydipsia oriental prescriptions on experimental diabetic rats, The Pharmaceutical Society of Korea, 38(4), 555 (1994).
8 Y. Z. Yang, Y. Z. Tang, and Y. H. Liu, Wogonoside displays anti-inflammatory effects through modulating inflammatroy mediator expression using raw 264.7 cells, Journal of Ethnopharmacology, 148(1), 271 (2013).   DOI
9 X. L. Li, A. G. Zhou, L. Zhang, and W.J. Chen, Antioxidant status and immune activity of glycyrrhizin in allergic rhinitis mice, International Journal of Molecular Sciences, 12(2), 905 (2011).   DOI
10 H. S. Lim, Y. J. Kim, C. S. Seo, S. R. Yoo, H. K. Shin, and S. J. Jeong, Chungsimyeonja-eum inhibits inflammatory responses in raw 264.7 macrophages and hacat keratinocytes, BMC Complementary and Alternative Medicine, 15(1), 371 (2015).   DOI
11 P. Perer, Text of the immune system, third edition, 1, Lifescience, Seoul (2011).
12 I. S. Kim, S. H. Lee, K. J. Lee, D. S. Lee, J. S. Park, and N. K. Lee, Text of essential immunology, 28, Daihak, Seoul (2017).
13 J. A. Smith, A. Das, S. K. Ray, and N. L. Banik, Role of pro-inflammatory cytokines released from microglia in neurodegenerative diseases, Brain Research Bulletin, 87(1), 10 (2012).   DOI
14 M. Hori, M. Kita, S. Torihashi, S. Miyamoto, K. J. Won, K. Sato, and H. Karaki, Upregulation of inos by cox-2 in muscularis resident macrophage of rat intestine stimulated with lps, American Journal of Physiology-Gastrointestinal and Liver Physiology, 280(5), 930 (2001).   DOI
15 C. A. Dinarello, Biologic basis for interleukin-1 in disease, American Society of Hematology, 87(6), 2095 (1996).
16 T. Kishimoto, S. Akira, M. Narazaki, and T. Taga, Interleukin-6 family of cytokines and gp130, American Society of Hematology, 86(4), 1243 (1995).
17 H. K. Kim, Role of erk/mapk signalling pathway in anti-inflammatory effects of ecklonia cava in activated human mast cell line-1 cells, Asian Pacific Journal of Tropical Medicine, 7(9), 703 (2014).   DOI
18 D. Y. Kim, Anti-allergic effect of sapindus mukorossi fruits extract in human mast cells, Journal of Investigative Cosmetology, 15(4), 389 (2019).
19 X. Xu, P. Yin, C. Wan, X. Chong, M. Liu, P. Cheng, J. Chen, F. Liu, and J. Xu, Punicalagin inhibits inflammation in lps-induced raw 264.7 macrophages via the suppression of tlr4-mediated mapks and nf-${\kappa}b$ activation, Inflammation, 37(3), 956 (2014).   DOI
20 T. Lawrence, C. Fong, The resolution of inflammation: anti-inflammatory roles for nf-${\kappa}b$, The International Journal of Biochemistry & Cell Biology, 42(4), 519 (2010).   DOI
21 M. Yoshizumi, T. Nakamura, M. Kato, T. Ishioka, K. Kozawa, K. Wakamatsu, and H. Kimura, Release of cytokines/chemokines and cell death in uvb-irradiated human keratinocytes, hacat, Cell Biology International, 32(11), 1405 (2008).   DOI
22 S. N. Lee, The antioxidant effect of rutin in human dermal fibroblasts damaged by reactive oxygen species, Asian Journal of Beauty & Cosmetology. 12(6), 831 (2014).
23 X. Xu, H. Li, X. Hou, D. Li, S. He, C. Wan, P. Yin, M. Liu, F. Liu, and J. Xu, Punicalagin induces nrf2/ho-1 expression via upregulation of pi3k/akt pathway and inhibits lps-induced oxidative stress in raw 264.7 macrophages, Mediators of Inflammation. 1, 15 (2015).   DOI
24 J. P. Roach, E. E. Moore, D. A. Partrick, S. S. Damle, C. C. Silliman, R. C. Mclntyre, and A. Banerjee, Heme oxygenase-1 induction in macrophages by a hemoglobin-based oxygen carrier reduces endotoxin-stimulated cytokine secretion, Shock, 31(3), 251 (2009).   DOI
25 N. Li, J. Alam, M. I. Venkatesan, A.E. Fernandez, D. Schmitz, E. D. Stefano, N. Slauther, E. K. Xiaorong wang, A. Huang, M. Wang, A. H. Miguel, and A.E. Nelj, Nrf2 is a key transcription factor that regulates antioxidant defense in macrophages and epithelial cells: protecting against the proinflammatory and oxidizing effects of diesel exhaust chemicals, Immunology, 173(5), 3467 (2004).   DOI