• Title/Summary/Keyword: Stress & strain measurement

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Requirement of Fur for the Full Induction of dps Expression in Salmonella enterica Serovar Typhimurium

  • Yoo, Ah-Young;Kim, Sam-Woong;Yu, Jong-Earn;Kim, Young-Hee;Cha, Jae-Ho;Oh, Jeong-Il;Eo, Seong-Kug;Lee, John-Hwa;Kang, Ho-Young
    • Journal of Microbiology and Biotechnology
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    • v.17 no.9
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    • pp.1452-1459
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    • 2007
  • The Dps protein, which is overexpressed in harsh environments, is known to playa critical role in the protection of DNA against oxidative stresses. In this study, the roles of Fur in the expression of the dps gene in Salmonella and the protection mechanisms against oxidative stress in Salmonella cells preexposed to iron-stress were investigated. Two putative Fur boxes were predicted within the promoter region of the S. typhimurium dps gene. The profile of dps expression performed by the LacZ reporter assay revealed growth-phase dependency regardless of iron-status under the culture conditions. The fur mutant, $_X4659$, evidenced a reduced level of ${\beta}$-galactosidase as compared to the wild-type strain. The results observed after the measurement of the Dps protein in various Salmonella regulatory mutants were consistent with the results acquired in the reporter assay. This evidence suggested that Fur performs a function as a subsidiary regulator in the expression of dps. The survival ability of Salmonella strains after exposure to oxidative stress demonstrated that the Dps protein performs a pivotal function in the survival of stationary-phase S. typhimurium against oxidative stress. Salmonella cells grown in iron-restricted condition required Dps for full protection against oxidative stress. The CK24 (${\Delta}dps$) cells grown in iron-replete condition survived at a rate similar to that observed in the wild-type strain, thereby suggesting the induction of an unknown protection mechanism(s) other than Dps in this condition.

Structural performance monitoring of an urban footbridge

  • Xi, P.S.;Ye, X.W.;Jin, T.;Chen, B.
    • Structural Monitoring and Maintenance
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    • v.5 no.1
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    • pp.129-150
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    • 2018
  • This paper presents the structural performance monitoring of an urban footbridge located in Hangzhou, China. The structural health monitoring (SHM) system is designed and implemented for the footbridge to monitor the structural responses of the footbridge and to ensure the structural safety during the period of operation. The monitoring data of stress and displacement measured by the fiber Bragg grating (FBG)-based sensors installed at the critical locations are used to analyze and assess the operation performance of the footbridge. A linear regression method is applied to separate the temperature effect from the stress monitoring data measured by the FBG-based strain sensors. In addition, the static vertical displacement of the footbridge measured by the FBG-based hydrostatic level gauges are presented and compared with the dynamic displacement remotely measured by a machine vision-based measurement system. Based on the examination of the monitored stress and displacement data, the structural safety evaluation is executed in combination with the defined condition index.

Accurate Measurement of Residual Stresses of Glass Rods by Photoelasticity (광탄성법에 의한 유리봉 잔류응력의 정밀측정)

  • Baek, Tae-Hyun
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.20 no.5
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    • pp.1524-1533
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    • 1996
  • Risidual stress of cylindrical glass rods are measured by photoelasticity to study the variation of stresses with respect to heat treatment temperatures. In order to measure the stresses accurately, fringe sharpening and multiplication techniques are applied to the determination of photoelastic fringe orders. Filon's separationmethod is used to resolve circumferential and redial stress ocmponents from isochromatic fringes which are the same as in-plane maximum shearing stresses. According to the photoelastic measurements, residual stress is increased as the heat treatment temperature of the rods is raised from $560^{\circ}C$ to $650^{\circ}C$ All the circumferential stress components are changed from tensile stresses to compressive ones at approximate $R_m$/$R_o$ = 0.6, where $R_o$/ is outer radius and $R_m$any measured radius. This analysis shows that residual stresses of the glass rods approach zero if the rods are heat-treated near the strain point.

Creep Analysis of Type 316LN Stainless Steel by Reference Stress Concept (참조응력 개념에 의한 316LN 강의 크리프 해석)

  • Kim, Woo-Gon;Kim, Dae-Whan;Ryu, Woo-Seog
    • Proceedings of the KSME Conference
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    • 2001.11a
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    • pp.123-128
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    • 2001
  • The creep constants which are used to the reference stress equations of creep damage were obtained to type 316LN stainless steel, and their determining methods were described in detail. Typical Kachanov and Rabotnov(K-R) creep damage model was modified into the damage equations with reference stress concepts, and the modified equations were applied practically to type 316LN stainless steel. In order to determine the reference stress value, a series of high-temperature tensile tests and creep tests were accomplished at $550^{\circ}C$ and $600^{\circ}C$. By using the experimental creep data, the creep constants used in reference stress equations could be obtained to type 316LN stainless steel, and a creep curve on rupture strain was predicted. The reference stress concept on creep damage can be utilized easily as a design tool to predict creep life because the process, which is quantified by the measurement of voids or micro cracks during creep, is omitted.

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The Unsaturated Stress Strain Behavior of CDG (Completely Decomposed Granite) Soils (완전 풍화된 화강풍화토의 불포화 응력-변형률 거동 특성)

  • Ham, Tae-Gew;Ahn, Tae-Bong
    • Journal of the Korean Geotechnical Society
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    • v.26 no.6
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    • pp.21-28
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    • 2010
  • Decomposed granite soil is the most common type of soils. The measurement of the stress-strain-strength behavior of anisotropic decomposed granite soils is very important for the deformation and stability analysis of slopes, retaining walls, excavations. A series of unsaturated-drained triaxial compression tests were performed to know unsaturated strength properties. The sample had three different angles of the axial (major principal) direction to the sedimentation plane (compaction plane): 0, 45 and 90 degrees. The compression strain of specimens subjected to an isotropic compression was strongly influenced by the sedimentation angle. In addition, the time dependence was independent of the sedimentation angle in relation to the deformation behavior during the secondary compression process. The effect of the sedimentation angle on the triaxial compression strength and deformation was clearly shown with low confining stress. The effect of the sedimentation angle on the compressive strength and deformation was more evident in saturated specimens. A new method of predicting the shear strength of unsaturated decomposed granite soils, considering compaction angles, was proposed.

New Austrian Tunneling Method (일본의 NATM시공)

  • Toyoki Kadoya
    • Explosives and Blasting
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    • v.9 no.4
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    • pp.22-31
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    • 1991
  • NATM technic had been applied to Nakaya tunnel of Sin kan express R.R lines in 1975. on the worst expandable geological conditions, application of NATM method was carried out good result. Measurement data which include convergencymeter, inclinometer, extensometor load-cell, strain gage data of shotcrete stress and steel arch. was explained with slides. Induced NATM technic has been improved since 1975 as follows, specially adhesive method of shotcrete instead of spray method such as tunnel swift lining Sliding press lining, clean lining by pumping and sweeping tote lining ets.

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Estimation of the Axial Stress in High-Tension Bolt by Acoustoelastic Method (음탄성법을 이용한 고장력 볼트의 축응력 평가)

  • Chun, Hae-Hwa;Lee, Tae-Hoon;Jhang, Kyung-Young;Kim, Noh-Yu
    • Journal of the Korean Society for Nondestructive Testing
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    • v.26 no.5
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    • pp.285-290
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    • 2006
  • The evaluation of clamping condition has been regarded as the main issue in the safety-maintenance of the clamped high-tension bolts. For this, this paper proposes a method to estimate the axial stress by measuring the TOF (Time-Of-Flight) of ultrasonic wave, which is based on the acoustoelasticity or the dependency of sound speed on the stress. In this method, however, the variation of sound speed within the range of stress induced under the field condition is very small, and thus the accuracy of the TOF measurement is important. We adopted the phase detection method using tone-burst ultrasonic wave to measure the precise TOF. In order to verify the usefulness of the proposed method experiments are carried out and the results were compared with the stress measured by the strain gage. The results show good agreement with each other, and from these we can conclude that the proposed method is highly useful fnr the evaluation of clamping condition in the clamped high-tension bolts.

Investigation of Strain Field on a Misfit Dislocation in a Strained Si Layer Using the CFTM Method (CFTM 방법을 이용한 Si 박막과 격자불일치 전위결함의 변형률 분포에 대한 고찰)

  • Chang, Wonjae
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.30 no.12
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    • pp.757-761
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    • 2017
  • The computational fourier-transform moire (CFTM) method has been briefly explained and this method was used to perform strain analysis of a misfit dislocation in a strained $Si/Si_{0.55}Ge_{0.45}$ layer. An essential advantage of the CFTM method is that it does not require unwrapping, such that errors due to improper unwrapping can be excluded. The analysis results revealed that the Si layer was grown with tensile stress on $Si_{0.55}Ge_{0.45}$ and lattice constant of the Si layer along the growth direction was 1.9% smaller than that of $Si_{0.55}Ge_{0.45}$. On the other hand, strain of the misfit dislocation in the strained $Si/Si_{0.55}Ge_{0.45}$ layer was maximum at the dislocation core due to an extra half-plane and the $e_{xx}$ and $e_{yy}$ values were positive and negative, respectively, along the direction of a burgers vector.

The High Temperature Deformation Behavior of Mechanically (기계적 합금화된 Al-8wt% Fe분말의 고온 변형거동)

  • Jo, Gwon-Gu;Lee, Do-In;An, In-Seop;Heo, Bo-Yeong;Jo, Jong-Chun;Kim, Seon-Jin;Mun, In-Hyeong
    • Korean Journal of Materials Research
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    • v.3 no.1
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    • pp.50-57
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    • 1993
  • Abstract The sintering behavior of the mechanically alloyed AI-8wt%Fe power during vacuum hot pressing was investigated and high temperature deformation behavior of the sintered specimen was studied through compression tests at various strain rates in the temperature range between 35$0^{\circ}C$ and 45$0^{\circ}C$. In 'addition, thermal stablity of the sintered specimem was examined by hardness measurement after annealing the spcimem for 60 hours in the temperature range of 30$0^{\circ}C$ ~50$0^{\circ}C$. The compressive stress increased rapidly with strain and reached the maximum point at the strain about 3%. With slight decrement after reaching the maximum point, the flow stress became constant up to the strain of 30% and it was considered to be due to equilibrium between work hardening and dynamic recrystallization. The hardness of the 60 hrs annealed specimens began to decrease rapidly at 40$0^{\circ}C$ .

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Ductile cracking simulation procedure for welded joints under monotonic tension

  • Jia, Liang-Jiu;Ikai, Toyoki;Kang, Lan;Ge, Hanbin;Kato, Tomoya
    • Structural Engineering and Mechanics
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    • v.60 no.1
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    • pp.51-69
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    • 2016
  • A large number of welded steel moment-resisting framed (SMRF) structures failed due to brittle fracture induced by ductile fracture at beam-to-column connections during 1994 Northridge earthquake and 1995 Kobe (Hyogoken-Nanbu) earthquake. Extensive research efforts have been devoted to clarifying the mechanism of the observed failures and corresponding countermeasures to ensure more ductile design of welded SMRF structures, while limited research on the failure analysis of the ductile cracking was conducted due to lack of computational capacity and proper theoretical models. As the first step to solve this complicated problem, this paper aims to establish a straightforward procedure to simulate ductile cracking of welded joints under monotonic tension. There are two difficulties in achieving the aim of this study, including measurement of true stress-true strain data and ductile fracture parameters of different subzones in a welded joint, such as weld deposit, heat affected zone and the boundary between the two. Butt joints are employed in this study for their simple configuration. Both experimental and numerical studies on two types of butt joints are conducted. The validity of the proposed procedure is proved by comparison between the experimental and numerical results.