• KSBB Journal
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• v.25 no.2
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• pp.167-172
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• 2010

Streptomyces scabiei producing phytotoxin called thaxtomin, which cause scab disease on economically important crops such as potato. For molecular genetics study of S. scabiei an effective transformation method was established based on conjugal transfer from Escherichia coli ET12567 (pUZ8002) using a phiC31-derived integration vector, pSET152, containing oriT and attP fragments. The high frequency was obtained on MS medium containing 50 mM $MgCl_2$. In addition, the sequence and location of the chromosomal integration attB site of S. scabiei was identified for the first time in the strains producing thaxtomin by the southern blot analysis of exconjugants and the sequencing of plasmid containing DNA flanking the insertion sites from exconjugant chromosome. Similar to the case of Streptomyces species, a single phiC31 attB site of S. scabiei is present within an ORF encoding a pirin-homolog.

• Journal of Microbiology
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• v.40 no.2
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• pp.178-181
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• 2002

Streptomycete isolator strain M0137 showed cytotoxic effect on THP-1 cells. One of the purified substances produced from the strain was identified as nonadecanoic acid. Morphological and physiological properties, phylogenetic analysis, and genomic fingerprinting of strain M0137 were determined. Strain M0137 showed a high similarity with Streptomyces scabiei, phenotypically and phylogenetically. In contrast, genomic fingerprinting and G+C content analysis revealed that strain M0137 could be distinguished from S. scabiei ATCC49173$\^$T/. We propose to name strain M0137 as Streptomyces scabiei Subsp.