• Title/Summary/Keyword: Streptomyces

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Isolation and Identification of Streptomyces aburabiensis Producing Cathepsin B Inhibitor (Cathepsin B 저해물질을 생산하는 Streptomyces aburabiensis의 분리 및 동정)

  • 박상진;이현숙;김인섭;김형태;윤성준;이계준
    • YAKHAK HOEJI
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    • v.39 no.3
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    • pp.297-305
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    • 1995
  • The aim of the present study was to develop strains of actinomycetes producing low molecular weight cathepsin B inhibitor. Among 700 isolates from soil samples, a strain of Streptomyces sp. SMF30 producing cathepsin B inhibitor showing specificity and heat stability was selected by an economical and effective screening method. 50 units characteristics for major cluster analysis and 34 units characteristics for minor cluster were tested and the data were analyzed numerically using the TAXON program. The Isolate SMF30 was identified as a strain of Streptomyces aburabiensis

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Isolation and Identification of an acidoduric Streptomyces sp. from Forest Soil (산림토양으로 부터 내산성 streptomyces sp. 균주의 분리 및 동정)

  • 김재헌;송도한
    • Korean Journal of Microbiology
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    • v.25 no.3
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    • pp.238-243
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    • 1987
  • In this study, an acidoduric Streptomyces strain was isolated and identified from acidic forest soil around Dankook University, Cheonan Campus. This isolated strain had rod-shaped, smooth, non-motile spore and the shape of spore chain was compact spiral. This structure appeared similar to the sporangium of the genus Streptosporangium but this strain proved to be a Streptomyces strain by an electron microscopic study and cell wall analysis. This strain showed a best growth on neutral medium, was also able to grow on the acidic media of pH 4.0 and pH 5.0. The color determination of this strain on various agar media and other physiological tests were carried out by ISP-methods. From these results, the isolated strain was considered to be Streptomyces mirabilis.

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Draft genome sequence of Streptomyces sp. P3 isolated from potato scab diseased tubers (감자 더뎅이병 이병괴경으로부터 분리한 Streptomyces sp. P3 균주의 유전체 해독)

  • Kang, Min Kyu;Park, Duck Hwan
    • Korean Journal of Microbiology
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    • v.54 no.2
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    • pp.158-160
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    • 2018
  • Streptomyces sp. P3 was isolated from potato scab diseased tubers in Pyeongchang, Gangwon-do, Republic of Korea in 2017. Here, we report the draft genome sequences of P3 with 9,851,971 bp size (71.2% GC content) of the chromosome. The genome comprises 8,548 CDS, 18 rRNA and 66 tRNA genes. Although strain P3 did not show pathogenicity both potato tuber assay and radish seedling assay, it possesses tomatinase (tomA) gene among conserved pathogenicity-related genes in well characterized pathogenic Streptomyces. Thus, the genome sequences determined in this study will be useful to understand for pathogenic evolution in Streptomyces species, which already adapted to potato scab pathogens.

groES Expression Related to Antifungal Activity of Streptomyces sp. SAR01 (Streptomyces sp. SAR01 균주에서의 항진균 관련 groES의 발현)

  • 이영근;김재성;조규성;장병일;추철형
    • Korean Journal of Microbiology
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    • v.38 no.3
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    • pp.162-167
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    • 2002
  • To analyse proteins and gene related to antifungal activity, SAR01 strain was isolated from a brown seaweed and identified as Streptomyces sp. by FAME(fatty acid methyl ester) analysis. Antifungal activity deficient mutant(SAR535) of Streptomyces sp. SAR01 was induced by gamma radiation$({60}^Co)$. It was found that 6 specific protein spots appeared only in SAR01 by 2-D electrophoresis analysis. Among them, a protein of 10 kDa had homology of 96% with 10 kD chaperonin cpn 10 (GroES) by Basic Local Alignment Search Tool(BLAST, NCBI) analysis. SAR535 transformants into which groES was transferred by electroporation revealed antifungal activity newly similar with SAR01 It suggested that groES be supposed to be related to the antifungal activity of Streptomyces sp. SAR01.

Antioxidative Effects of Cultivation of Streptomyces sp. BH-405 Isolated from Marine Origin (해양에서 분리한 Streptomyces sp. BH-405 배양액의 항산화 효과)

  • 류병호;이영숙;양승택
    • KSBB Journal
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    • v.15 no.2
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    • pp.150-155
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    • 2000
  • Antioxidative activity of c비ture of Streptomyces sp. BH-405 was investigated. After removal of pellets of Streptomyces sp. B BH-405, antioxidative substances were is미ated and suc$\infty$sively purified from culture of Streptomyces sp. BH-405 by by thin | layer chromatography $\pi$LC) or silica gel column chromatography. The fraction 3 obtained from ethylether fractionation of the C culture appeared highest level of anti oxidative activity as determined by thiocyanate method. Band 2 obtained by further P purification of this fraction showed higher anti oxidation level than that of same concentration of dl- $\alpha$ -tocopherol, butylated h hydroxy anisole (BHA). The band 2 showed higher rate of 1, 1.diphenyl 2-picrylhydrazyl (DPPH) decolorization than dl-$\alpha$-tocopherol. In the rat liver microsomes, band 2 rapidly inhibited lipid peroxidation which was initiated enzymatically by r reduced nicotinamide adenine dinucleotide phosphate (NADPH) or non-enzymatically by Fenton’s reagent. Band 2 inhibited on | lipid peroxidation of mitochondria or the linoleic acid hydro peroxide induced peroxidation system. It is concluded that band 2 obtained by fractionation of Streptomyces sp. BH-405 cultivation contained antioxidants with the capacity to inhibit oxidative m modification.

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Turfgrass Probiotics Reduce Population of Large Patch Pathogen and Improve Growth of Zoysiagrass (유용미생물 처리에 따른 들잔디 재배지의 갈색퍼짐병 병원균 감소와 잔디생육 촉진 효과)

  • Bae, Eun-Ji;Cheon, Chang Wook;Hong, A-Reum;Lee, Kwang-Soo;Kwak, Youn-Sig
    • Weed & Turfgrass Science
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    • v.6 no.3
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    • pp.249-261
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    • 2017
  • To prevent large patch disease, caused by Rhizoctonia solani AG-2-2, in zoysiagrass a fungicide, Tebuconazole and three microbial agents Streptomyces sp. Burkholderia sp. and Streptomyces sp. S8 were applied in commercial turfgrass cultivation field in Sanchung, Gyeongnam, Korea. All treatments showed 50% reduced the pathogen population in thatch layer throughout the yearly cultivation period. Not only reduced the pathogen population, Tebuconazole, Streptomyces sp. Burkholderia sp. and Streptomyces sp. S8 treatment also enhanced turfgrass growth, chlorophyll and proline content. Malondialdehyde contents in each treatment was reduced from 6.2~28.9% when compared with the control. Taken together, reduction of pathogen population in soil lowered the disease incidence or severity, and allowed the turfgrass developed as normal condition. The results suggested that the selected microbial agents may use as biological control and growth promotion agents for the Zoysia turfgrass.

Identification of Streptomyces species antagonistic to Fusarium solani or Cylindrocarpon destructans causing ginseng root rots (인삼근부(人蔘根腐) 병원균(病原菌), Fusarium solani 및 Cylindrocarpon destructans에 길항적(拮抗的)인 Streptomyces species의 분류동정(分類同定))

  • Shim, Jae-Ouk;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.19 no.1
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    • pp.66-73
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    • 1991
  • Among 110 isolates of actinomycetes isolated from ginseng pathogen-suppressive soils, the three actinomycetes showing the effective controls to Fusarium solani or Cylindrocarpon destruc­tans causing ginseng root rots were identified according to their morphological, cultural and physio­logical characteristics on various culture media. Spore chains of K 6-2, S 2-1 and Y 2-2 were Spira (S), Retinaculum-apertum (RA) and Rectus-flexibilis (RF), respectively. Spore surfaces of K 6-2 were spiny, whereas S 2-1 and Y 2-2 were all smooth. Aerial mass colors of 3 isolates were gray series. As a result of various tests, they were identified as Streptomyces variabilis, Streptomyces virgi­niae and Streptomyces griseo/us, respectively.

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The Study of Enzymes Produced by Streptomyces halstedii ssp. scabies SA1-27 and Streptomyces violaceusinger C1-6 Which Have Good Lignolytic Activity (리그닌의 분해가 우수한 Streptomyces halstedii ssp. scabies SA1-27과 Streptomyces violaceusinger C1-6에서 생성되는 효소들에 관한 연구)

  • Kim, Tai-Jeon
    • Korean Journal of Clinical Laboratory Science
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    • v.38 no.2
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    • pp.87-93
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    • 2006
  • This study was done to know a kind and change (transition) of enzymes produceed by Streptomyces halstedii ssp. scabies SA1-27 and Streptomyces violaceusinger C1-6 which showed good lignolytic activity and a good decolorization ratio of remazol brilliant blue R(RBBR) dye. These strains were isolated from soil and identified by the author. The basal medium containg 0.2% glucose was used to measure enzyme activity, Lignin peroxidase 1 (Lip 1) was measured by the methods of Choi, and Bourbonnais and Paice. Lignin peroxidase 2 (Lip 2) was measured by the methods of Ishida et al and Ramachandra et al using 2.4-dichlorophenol(2.4 DCP), manganese peroxidase(Mnp), veratryl alcohol oxidase (VAO), and laccase. They were measured by each of the methods of Choi and Paszczynski et al, and Bourbonnais and Paice, and De Jong et al. In the results, the kind of enzymes produced by Streptomyces halstedii ssp. scabies SA1-27 were Lip 1, Lip 2, VAO, and laccase, and their activities indicated the highest value as each 4.95 nmol/mg protein, $8.45({\times}100^{-3})unit$, 10.25 nmol/mg protein, 9.20 nmol/mg protein on the sixth day of the culture and decreased gradually over time. The kind of enzymes produced by Streptomyces violaceusinger C1-6 were Lip 1, Lip 2, Mnp, VAO, and laccase, and their activities indicated the highest value as each 4.90 nmol/mg protein, $13.85({\times}100^{-3})unit$, 3.10 nmol/mg protein, 11.30 nmol/mg protein, 4.45 nmol/mg protein on the sixth day of the culture and decreased gradually over time. Consequently, the author knew the fact that there were few differences in the kind and quantity of enzymes produced by the two Streptomyces strains, but all enzyme activities indicated the highest value on the sixth day of the culture and decreased gradually over time.

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INHIBITION OF ARTIFICIAL PLAQUE BY MUTANASE PRODUCED FROM Streptomyces exfoliatus (Streptomyces exfoliatus가 생성하는 mutanase에 의한 인공치태 억제 작용)

  • Song, Do-Won;Yang, Kyu-Ho;Chung, Jin;Oh, Jong-Suk
    • Journal of the korean academy of Pediatric Dentistry
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    • v.24 no.2
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    • pp.449-459
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    • 1997
  • The main component of dental plaque is the mutan containing the a-1,3 bond. The following results were obtained by using a blue mutan to assess the factors affecting the mutan-digesting activity of Streptomyces exfoliatus isolated from soil. A clear zone was produced by mutanase-producing Streptomyces exfoliatus on the minimal essential agar containing blue mutan. Streptomyces exfoliatus digested more blue mutan in the minimal essential broth at pH 7.0 than at pH 5.5 or 8.5. Streptomyces exfoliatus digested more blue mutan at $37^{\circ}C$ than at $32^{\circ}C$ or $42^{\circ}C$ (P<0.05). When the concentration of $CaCl_2$ was increased in the minimal essential broth, the digestion of blue mutan was increased (P<0.05). The optimal concentration of KCl was 10mM to digest blue mutan, but a similar amount of blue mutan was digested at the range of 0.1mM to 6.4mM of $MgCl_2$. When the culture supernatant of Streptomyces exfoliatus was mixed with 2X brain heart infusion broth containing 0.5% yeast extract and 10% sucrose, less artificial plaque was formed by Streptococcus mutans on the orthodontic wire (P<0.05). These results indicated that the secretion of mutanase was identified in culture supernatant of mutan-digesting Streptomyces exfoliatus, suppressing the formation of artificial plaque by Streptococcus mutans.

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Studies on the Cell Immobilization of Alkalophilic Streptomyces sp. B-2 for the Glucose Isomerization (포도당 이성화를 위한 Alkalophilic Streptomyces sp. B-2의 균체 고정화에 관한 연구)

  • 이은숙
    • The Korean Journal of Food And Nutrition
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    • v.11 no.3
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    • pp.319-322
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    • 1998
  • The whole cell of alkalophilic Streptomyces sp. B-2 which produce glucose isomerase was immobilized by entrapment method for the effective production of high fructose syrup. The highest immobilized activity was achieved when the enzyme was bound to 2% $textsc{k}$-carrageenan. Immobilized glucose isomerase the pH optimum was about pH 7.5~8.5. Immobilization of alkalophilic Streptomyces sp. B-2 on 2% $textsc{k}$-carrageenan at 7$0^{\circ}C$ showed an increase in glucose isomerase activity. GI activity of immobilized cells was maximum Co2+ concentration 10-3M, Mg2+ concentration 10-3M.

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