• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.6
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• pp.63-71
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• 2018

Strands with ultimate strength of 2,400 MPa was developed and applied in the KCI Code and the KS standard. A high-strength prestressed strand to be applied to a structure, a suitable anchorage system should be used together. Recently, a post tension anchorage for 2,400 MPa strands was developed. but there is not much research on performance evaluation. Therefore, in this study, structural analysis of local zone with 9 strands, 15 strands, and 19 strands anchorage were investigated respectively, which are most widely used for post tensioning anchorages with 2,400 MPa strands, according to PTI anchorage zone design method, and Load transfer performance from ETAG013 and/or KCI-PS101 was evaluated. Furthermore, the adequacy of the test was also analyzed by nonlinear numerical analysis. As results, the anchorages with 2,400 MPa strands satisfied the structural performance of the local area and satisfied the load transfer performance condition.

• Journal of Mechanical Science and Technology
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• v.15 no.4
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• pp.473-481
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• 2001

By depicting the transfer of heat and combustion reaction to take place within thin gas layers close to the propellant surface burning in a steady-state fashion, a mathematical equation has been deduced to describe the burning rate of solid propellant as a function of initial grain temperature and chamber pressure. It has been also assumed that chemical reaction could take place in premixing-diffusing zone but were carried out mainly in the reaction-flame zone. All these phenomena taken place in each zone of combustion have been assumed to be steady-state. In the present investigation, the equation, γ=$\kappa$$.$(1/R(T(sub)i+C))(sup)n$.$exp(-E(sub)a/R(T(sub)i+C))(P/z) is being presented and it is compared with experimental data. The proposed model has been tested and evaluated vis-a-vis strand burner data for three different propellants based on CTPB, and it has been found that the deviation of the computed burning rates from the measured rates ranged up to 2%.

• BMB Reports
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• v.41 no.10
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• pp.739-744
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• 2008

Turnip yellow mosaic virus (TYMV) is a positive strand RNA virus that infects mainly Cruciferae plants. In this study, the TYMV genome was modified by inserting an extra subgenomic RNA promoter and a multiple cloning site. This modified TYMV was introduced into Nicotiana benthamiana using a Agrobacterium-mediated T-DNA transfer system (agroinfiltration). When a gene encoding $\beta$-glucuronidase or green fluorescent protein was expressed using this modified TYMV as a vector, replication of the recombinant viruses, especially the virus containing $\beta$-glucuronidase gene, was severely inhibited. The suppression of replication was reduced by co-expression of viral silencing suppressor genes, such as tombusviral p19, closteroviral p21 or potyviral HC-Pro. As expected, two subgenomic RNAs were produced from the recombinant TYMV, where the larger one contained the foreign gene. An RNase protection assay revealed that the recombinant subgenomic RNA was encapsidated as efficiently as the genuine subgenomic RNA.

• Structural Engineering and Mechanics
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• v.69 no.4
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• pp.371-381
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• 2019

A hybrid prestressed segmental concrete (HPSC) girder utilizing low carbon materials was developed in this paper. This paper introduces the hybrid prestressing concept of pre-tensioning the center segment and assembling all segments by post-tensioning, as well as the development process of the low carbon HPSC girder. First, an optimized mix proportion of 60 MPa high strength concrete containing high volume blast furnace slag was developed, then its mechanical properties and durability characteristics were evaluated. Second, the mechanical properties of 2,400 MPa high strength prestressing strands and the transfer length characteristics in pre-tensioned prestressed concrete beams were evaluated. Third, using those low carbon materials and the hybrid prestressing concept, the HPSC girders were manufactured, and their structural performance was evaluated. A 30-m long HPSC girder for highway bridges and a 35-m long HPSC girder for railway bridges were designed, manufactured, and structurally confirmed as having sufficient strength and safety. Finally, five 35-m long HPSC girders were successfully applied to an actual railway bridge for the first time.

• Genomics & Informatics
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• v.18 no.3
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• pp.32.1-32.7
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• 2020

The mitochondrial genome of a species is an essential resource for its effective conservation and phylogenetic studies. In this article, we present sequencing and characterization of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba collected from Khulna region of Bangladesh. The complete mitochondrial genome was 16,597 bp in size, which formed a circular double-stranded DNA molecule containing a total of 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes) with two non-coding regions, an origin of light strand replication (OL) and a displacement loop (D-loop), similar structure with other fishes of Teleostei. The phylogenetic tree demonstrated its close relationship with labeonine fishes. The complete mitogenome of Cirrhinus reba (GenBank no. MN862482) showed 99.96% identity to another haplotype of Cirrhinus reba (AP013325), followed by 90.18% identity with Labeo bata (AP011198).

• Journal of the Korea Concrete Institute
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• v.22 no.6
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• pp.753-760
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• 2010

Ultra high performance concrete (UHPC), is characterized by its high compressive strength and advanced tensile behavior that is much superior to those of conventional concrete. In order to apply this new material in practice, the bond characteristics of UHPC were evaluated in this study. Pull-out tests between UHPC and deformed steel rebar were carried out according to the modified RILEM test method, and were verified by finite element analysis. From the test results showed that UHPC presents 5 to 10 times higher bond strength compared to normal strength concrete, this study suggested remarkably reduced development length and concrete cover comparing to existing specifications. The test results of 700 MPa high strength steel rebar demonstrated the applicability of high strength steel to UHPC. In addition, the transfer length measurements of seven-wire strand in UHPC specimens indicated that the transfer length limit set by the current design code is very conservative for UHPC.

• Journal of Embryo Transfer
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• v.25 no.2
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• pp.111-116
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• 2010

Sperm chromatin integrity is essential for successful fertilization and development of an embryo. Reported here is a quantification of DNA fragments which is intimately associated with reproductive potential to provide one of criteria for sperm chromatin integrity. Three sperm populations were considered: CONTROL (no treatment), UV irradiation (48mW/$cm^2$, 1h) and $H_2O_2$ (oxidative stress induced by hydrogen peroxide, 10 mM, 50 mM and 100 mM). DNA fragments in boar sperm were evaluated by using ligation-mediated quantitative real-time polymerase chain reaction (LM-qPCR) assay, which relies on real-time qPCR to provide a measure of blunt 5' phosphorylated double strand breaks in genomic DNA. The results in agarose gel electrophoresis showed no significant DNA fragmentation and no dose-dependent response to $H_2O_2$. However, the remarkable difference in shape and position was observed in melting curve of LM-qPCR. This result supported that the melting curve analysis of LM-qPCR presented here, could be more sensitive and accurate than previous DNA fragmentation assay method.

• Molecules and Cells
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• v.19 no.2
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• pp.246-255
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• 2005

Retroviral integrases insert viral DNA into target DNA. In this process they recognize their own DNA specifically via functional domains. In order to analyze these functional domains, we constructed six chimeric integrases by swapping domains between HIV-1 and HFV integrases, and two point mutants of HFV integrase. Chimeric integrases with the central domain of HIV-1 integrase had strand transfer and disintegration activities, in agreement with the idea that the central domain determines viral DNA specificity and has catalytic activity. On the other hand, chimeric integrases with the central domain of HFV integrase did not have any enzymatic activity apart from FFH that had weak disintegration activity, suggesting that the central domain of HFV integrase was defective catalytically or structurally. However, these inactive chimeras were efficiently complemented by the point mutants (D164A and E200A) of HFV integrase, indicating that the central domain of HFV integrase possesses potential enzymatic activity but is not able to recognize viral or target DNA without the help of its homologous N-terminal and C-terminal domains.

• Journal of Microbiology and Biotechnology
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• v.20 no.6
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• pp.968-973
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• 2010

In order to study its biochemical properties, the integrase (IN) protein of feline foamy virus (FFV) was overexpressed in Escherichia coli, purified by two-step chromatography, (Talon column and heparin column), and characterized in biochemical aspects. For the three enzymatic reactions of the 3'-processing, strand transfer, and disintegration activities, the $Mn^{2+}$ ion was essentially required as a cofactor. Interestingly, $Co^{2+}$ and $Zn^{2+}$ ions were found to act as effective cofactors, whereas other transition elements such as $Ni^{2+}$, $Cu^{2+}$, $La^{3+}$, $Y^{3+}$, $Cd^{2+}$, $Li^{1+}$, $Ba^{2+}$, $Sr^{2+}$, and $V^{3+}$ were not. Regarding the substrate specificity, FFV IN has low substrate specificities as it cleaved in a significant level prototype foamy virus (PFV) U5 LTR substrate as well as FFV U5 LTR substrate, whereas PFV IN did not. Finally, the 3'-processing activity was observed in high concentrations of several solvents such as CHAPS, glycerol, Tween 20, and Triton X-100, which are generally used for dissolution of chemicals in inhibitor screening. Therefore, in this first report showing its biochemical properties, FFV IN is proposed to have low specificities on the use of cofactor and substrate for enzymatic reaction as compared with other retroviral INs.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.7 s.178
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• pp.1650-1660
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• 2000

Continuous casting process has been adopted increasingly in recent years to save both energy and labor. It has experienced a rapid development in the production of semi-finished steel products, rep lacing the conventional route of ingot casting and rolling. In order to achieve this merit, however, more studies about the mechanism between roll and slab are needed. In this paper, a dynamic bulging in steel cast slabs was simulated by considering the solidification and heat transfer. This study is to prevent internal cracks of a slab in a bending and unbending zone. The value of moving strand shell bulging between two supporting rollers under the ferrostatic pressure and slab-self weight has been calculated in terms of creep and elasto-plasticity. The strain and strain rate distributions in solidified shell undergoing a series of bulging are calculated with working boundary conditions.