• 콘크리트학회논문집
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• 제22권6호
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• pp.753-760
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• 2010

초고성능 콘크리트(ultra high performance concrete, UHPC)는 종래의 보통 콘크리트와 다른 새로운 재료로써 높은 강도와 향상된 인성을 그 특징으로 한다. 이러한 새로운 재료의 활용을 위하여 이 연구에서 초고성능 콘크리트의 부착 성능을 평가하고자 하였다. 수정된 RILEM 방법을 사용하여 초고성능 콘크리트와 이형 철근의 인발실험(pull-out test)을 수행하였으며 보통 콘크리트와 비교하여 5~10배에 달하는 부착강도를 확인하여 기존의 설계 기준에 비하여 현저하게 감소한 정착길이와 피복 두께를 제안하였다. 700 MPa급 고장력 철근의 실험 결과의 비교로부터 초고성능 콘크리트에서 고강도 철근 활용의 유효성을 확인하였다. 강연선의 응력전달길이 측정실험을 통하여 현재 전달길이 기준이 UHPC의 경우 매우 보수적이라는 것을 확인하였다. 또한 유한요소해석을 통하여 실험 결과를 검증하였다.

• 콘크리트학회지
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• 제8권4호
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• pp.171-179
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• 1996

PC판넬 조립식 구조의 수직.수평접합부의 유용한 접합형태에 따라 접합부 전단내력을 상승시킬 수 있는 접합부 설계의 기본 형태를 제시하는데 연구의 목적이 있다. 시험체는 수직접합부의 경우 전단키의 개수 및 보강철물의 종류에 변수를 주고 수평접합부의 경우에는 전단키의 갯수 및 가력방향에 변수를 주어 총 22개의 시험체를 제작하여 실험하였다. 결론적으로 수직접합부의 경우, 수평보강철물이 있는 시험체는 기존의 다른 실험결과와 마찬가지로 수평보강철물이 없는 시험체에 비하여 연성이 크게 나타났다. EH한 와이어로프와 원형철근 두 종류의 보강철물을 사용하여 실험한 결과 와이어 로프를 사용한 시험체는 원형철근을 사용한 시험체와 최대내력 및 전단강성 등 전체적인 구조거동이 거의 비슷하게 나타났다.

• 한국지능시스템학회논문지
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• 제14권5호
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• pp.545-550
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• 2004

생태학, 환경공학, 임상진단 등 생물학 분야에서 미생물의 다양성 연구의 중요성이 대두되고 그 연구가 점증하고 있다. 특히 16S rRNA를 분자지표로한 DNA 염기서열 분석방법이 널리 사용되고 있다. 본 논문에서는 16S rRNA의 염기서열 분석과정을 각 단계별로 자동화하고, 생물학자들의 결과 판단이나 사용상의 편의를 도모하기 위하여 웹기반의 미생물 다양성 분석 어플리케이션을 개발하였다. 이를 위하여 단계별 자동화 및 인터페이스 개발에 적합한 폴더-프로세스-필터 모델을 고안하고 적용하였다. 제공되는 생물정보분석도구는 서열입력, 서열방향교정, 다중서열정렬 및 가시화, 서열동정 등의 분석이 있으며, 각 결과는 계통분류도구와 호환 가능하도록 하였다. 또한 신생아의 장내 세균총에 대한 분석을 수행하여 개발된 도구의 유용성을 확인하였다. 개발된 웹 어플리케이션은 리눅스 시스템 상에서 Perl 과 CGI를 이용하였으며, http://home.pusan.ac.kr/～genome/tools/rat.htm으로 접속하여 사용할 수 있다.

• 대한본초학회지
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• 제21권4호
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• pp.115-121
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• 2006

Objectives : For the purpose of developing new anti-HIV agents from natural sources, the extracts of Actinidia arguta were tested for their inhibitory effects on essential enzymes as the reverse transcriptase (RT), protease and ${\alpha}-\;glucosidase$. And we predicted inhibition activity of major compounds of Actinidia arguta using Quantitative Structure Activity Relationships (QSAR). Methods : In this assay the activity of HIV-1 reverse transcriptase is measured as the formation of a strand of copy-DNA (cDNA) using RNA as a template. The activity of HIV-1 protease is measured as the cleavage of an oligopeptide by HIV-1 protease. Results : In the anti-HIV-1 RT using Enzyme Linked Oligonucleotide Sorbent Assay (ELOSA) method, water extracts (100ug/ml) of stem and leaf showed strong activity of 93.9% and 91.9%, respectively. In the HIV-1 protease inhibition assay, aqueous stem extract inhibited the activity of the enzyme to cleave an oligopeptide, resembling one of the cleavage sites in the viral polyprotein which can only be processed by HIV-1 protease with 56.8%. In the ${\alpha}-glucosidase$ inhibition assay, aqueous stem extract showed activity of 73.1%. Conclusion : We found out this result, for these samples it is possible that the inhibition of the viral replication in vitro is due to the inhibition at least one of RT and ${\alpha}-glucosidase$. It would be of great interest to identify the compounds which are responsible for this inhibition, since all therapeutically useful agent up to date are RT, PR and ${\alpha}-glucosidase$ inhibitors.

• 콘크리트학회논문집
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• 제14권2호
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• pp.156-163
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• 2002

우리나라에서 교량이나 주차장 슬래브용으로 중공 슬래브를 활용한 예는 거의 없었다. 본 연구는 국내에서 생산중인 중공슬레브의 길이가 가장 큰 고성능 중공슬래브를 재설계하고, 그 적용성을 검토한 것이다. 이를 위해 최대 깊이 315 mm인 고성능 중공슬래브에 대하여 12.7 mm 슬래브 하부강선 10개와 상부강선을 4개를 배근한 중공슬래브 80mm 토핑콘크리트를 타설한 4개의 실험체에 휨실험을 실시하여, 차량 등을 위한 고하중에서의 활용성과 슬래브의 합성작용에 대한 검토를 병행하였다. 이 실험에서의 중공슬래브는 10 m-경간 슬래브에서 설계활하중 1,000 kgf/$m^2$를 고려할 때 강도설계에 적합한 연성적 휨파괴 거동을 보여주었다. 또한 슬래브와 토핑콘크리트의 합성거동을 위하여 사용한 직사각형 전단키와 원형 전단키는 모든 단부에서 순수 휨파괴까지 어떠한 미세 균열도 발생하지 않았으므로, 누 종류의 키는 전단키로 충분한 내력을 발휘한 것으로 판단된다.

• Journal of Ginseng Research
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• 제7권2호
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• pp.172-192
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• 1983

Light environment and growth of Panax ginseng In habitat and cultivation under natural shade were renewed. Grower's experiences on ginseng stand In relation to light environment were assessed. Change of shading method and grower's concepts on light requirement of ginseng plant in Korea, Manchuria and Japan was counted. Growth of wild ginseng was better under rich light. Optimum crown density index appeared to be 0.7 In natural habitat and 0.4 to 0.7 for the cultivation in forest. Change of light Intensity in forest was greatest in May and reached near to constant value (from 40% to 3% for broad leaf deciduous forest and loom 4% to 2% for pine forest). Insufficient light condition induced long and thin stem, poor flowering and seed bearing, and sequent dormancy. Relation between light and ginseng strand was not clear but light Interception with cool weather was effective. Topography and orientation of bed have been deeply considered for light environment. Panel or bark of won were used for shading in deep forest manly In Manchuria while reed blind and straw thatch were used in Korea. Kinds and number of shades material and seasonal or daily schedule have been simplified probably by labor pressure due to eulargement of plantation. Post height has been greater while width of roof, bed and ditch has changed lisle. Scientific survey in the past omitted important light control methods (complete light hardening etc) which has been practiced in specific areas. The concept and technique of light control in the past in Korea were delicate and intensive including the control of temperature and moisture. For the application of old concept in modem cultivation precise Investigation of the technique including the measurement of light, temperature and moisture is needed.

• Molecular & Cellular Toxicology
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• 제3권3호
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• pp.195-197
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• 2007

Currently praziquantel is used for treatment of not only clonorchiasis but also other trematodes and cestodes. But cure rate of praziquantel is just 60-80% for most trematodes. It needs for the treatment-failed patients to have more drugs. The cause of failure of treatment is not studied. We just know that the blood level of praziquantel is severely different among the people. We guess that this factor may influence the results of treatment. In an endemic area of human clonorchiasis in Heilongjiang Providence, China, 78 subjects were selected for the study. Three doses of 25 mg/kg (total 75 mg/kg) of praziquantel were administered to 78 clonorchiasis patients. After three weeks of treatment, stool examination was undertaken once again to confirm the cured and uncured subjects. To analyze SNP (single nucleotide polymorphism) of CYP3A5 PS2-1, CYP3A5 PS2-2, and CYP3A5*6, PCR method was done with specifically designed primers. The mutation rates of all sites were not significant statistically. The number of subjects was too small, so we need more subjects and other delivery proteins of bile ducts (ex. MRP etc.) were also considered for effects of praziquantel. We analyzed, for the first time, the entire CYP3A5 gene in a French population, using a polymerase chain reaction- single strand conformational polymorphism (PCR-SSCP) strategy.

• 대한수의학회지
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• 제38권3호
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• pp.565-573
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• 1998

To study the specific tools for the diagnosis of Newcastle disease virus (NDV) in chicken, polymerase chain reaction (PCR) and its presumable conditions were evaluated for the detection of hemagglutinin-neuraminidase (HN) gene of NDV RNA. For these purposes, Kyojeongwon strain of the NDV was propagated in allantoic cavity of SPF embryonating chicken eggs, and viral RNA was extracted from fractionated virus after the allantoic fluids were ultracentrifuged with sucrose gradient. The first-strand cDNA was then made for the HN gene of NDV RNA by reverse transcription at $42^{\circ}C$ for 1 hour using specific primer complementary to the HN gene. The single-stranded cDNA was used as template in the PCR of the HN-DNA, and various conditions of the PCR were evaluated to set up method for the specific detection of the HN-DNA. The PCR conditions promising for the detection of HN gene consist of preheating at $94^{\circ}C$, 5 min, 30 cycles of denaturation at $94^{\circ}C$, 1 min, annealing at $55^{\circ}C$, 1 min and polymerization at $72^{\circ}C$, 2 min, and a cycle of extension at $72^{\circ}C$, 5 min. when NDVs of allantoic fluids without fractionation were applied to the above PCR condition, the HN genes were detected effectively not only from Kyojeongwon but from other velogenic strains such as Herts and a field isolate.

• Journal of Gastric Cancer
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• 제3권4호
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• pp.206-213
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• 2003

Purpose: The clinical implication of p53 mutation in gastric cancer is still unclear, as shown by the discordant results that continue to be reported in the literature. Materials and Methods: To assess p53 gene mutation, tumor p53 overexpression, and serum anti-p53 antibody, we employed a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis, an immunohistochemistry using monoclonal antibody DO-7, and an enzymelinked immunosorbent assay (ELISA), respectively. Results: Of 169 surgical specimens of gastric cancer, mutation at exon $5\∼8$ of the p53 was identified in 33 ($19.5\%$) and was significantly correlated with lymph node metastasis. Overexpression of p53 was found in 62 specimens ($36.7\%$) and had a significant correlation with tumor differentiation. Serum anti-p53 antibody was positive in 18 patients ($10.7\%$). Twenty-three of the mutated tumors ($69.7\%$) and 39 of the non-mutated tumors ($28.7\%$) displayed immunoreactivity. Twelve of the immunopositive tumors ($19.4\%$) and 6 of the immunonegative tumors produced anti-p53 antibody. These differences were statistically significant (P<0.001 and P=0.005, respectively). There was no significant difference in survival according to the mutation of p53. Conclusion: Mutation and overexpression of p53 can be easily detected by immunohistochemistry. However, standardization of the immunohistochemical staining method, as well as guidelines for interpreting the stained result, will produce concordant results and thereby improve clinical application.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.734-740
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• 2005

The blood samples were collected from dairy cows at the same milking stage. The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5'flanking region of the hsp70 gene. The mRNA expression levels of HSP70, HSF1, Bcl-2 and Bax-$\alpha$ at different daily-mean-temperature were analyzed by relative quantitative RTPCR. The DNA content, cell phase and the ratio of apoptosis of lymphocytes in peripheral blood of dairy cattle at different daily-meantemperature were determined by FCM. The PCR-SSCP products of primer pair 1 showed polymorphisms and could be divided into four genotypes: aa, ab, ac, cc, with the cis-acting element (CCAAT box) included. Mutations in the hsp70 5'flanking region (468-752 bp) had different effects on mRNA expression of HSP70, HSF1, Bcl-2 and Bax-$\alpha$. The ac genotypic cows showed higher expressions of HSP70mRNA, HSF1mRNA and Bcl-2mRNA/Bax-$\alpha$mRNA and lower ratio of apoptosis. These mutation sites can be used as molecular genetic markers to assist selection for anti-heat stress cows.