• Proceedings of the Computational Structural Engineering Institute Conference
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• 1998.04a
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• pp.149-156
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• 1998

In suspension bridges, the axial farces in the wires are transferred by side pressure on the semicircular face, and further from the strand shoe through rods to a base plate fixed to the massive concrete part of the anchor block. For prefabricated strands the most common way of anchoring is by socketing the ends of the strands. In this study, strand shoe and hanger socket are analyzed far various load conditions using Finite Element Method. The finite element models are built using MSC/PATRAN and analysis is carried out using MSC/NASTRAN. Results are again completely processed using MSC/PATRAN. From the results of the analysis, trends of deformation and stress distribution are reviewed and important factors to consider in the design of strand shoe and hanger socket are discussed.

• The Journal of Korea Robotics Society
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• v.17 no.2
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• pp.178-182
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• 2022

Post-tension duct in nuclear reactor containment building is filled with grease to prevent steel strand from corroding. If grease leaks by break of duct, steel strand will corrode and cause problem in building safety. Therefore, grease leak should be checked preventatively. But currently used method is inefficient, since it has to remove grease and strand to check. And other methods for checking post-tension dust are not well-researched. In this paper, we develop screw-propelled robot that can move in grease and detect grease duct directly. Also, we make the test environment that is similar to real post-tension duct of containment building and test robot in that environment to verify that our robot is feasible in the post-tension duct. The robot can move forward and backward in grease duct by twin screw mechanism and has mount for sensors to detect grease leakage and strand corrosion.

• Proceedings of the Korea Concrete Institute Conference
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• 2003.11a
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• pp.207-210
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• 2003

As reviewing of current trend on PC connection details, owing to effective stress transfer in the connection, it grow to increase that use of mechanical splices, reinforcements or welded splices, and prestressing. However such devices as reinforcement, mechanical splices entail not only more cost resulted from materials but also extra construction process so as to cause PC used method to lower competition against conventional method. Therefore more enhanced connection details which help working process simplified and construction cost reduced. In this research, as replace 9.3mm 7strand for reinforcement, it is attempt to devise connection detail which makes workability improve and confirm effective stress transfer in the region of connection. The experimental research is proceeded by partial tension test of specimen. The splice lengths of 7strand is decided to be variations. The flexural capability is verified to depend on spice length. An an appropriate splice length could be also determined as a precedent research on improving PC connection detail.

• BMB Reports
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• v.32 no.6
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• pp.529-534
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• 1999

Asymmetric PCR and single-strand conformation polymorphism (SSCP) methods were combined to analyze human leukocyte antigen (HLA)-DRB allele polymorphism. Asymmetric PCR amplification was applied to generate single-stranded DNA (ssDNA) using the nonradioactive oligonucleotide primers desinged for the polymorphic exon 2 region. The conformational differences of ssDNAs, depending on the allele type, were analyzed by nondenaturing polyacrylamide gel electrophoresis and visualized by ethidium bromide staining. The ssDNAs were clearly separated from double-stranded DNA without interference and obviously migrated depending on their allele type. This method was applied to the genomic DNA either from homozygous or from heterozygous cell lines containing the DR4 allele as template DNA using DR4-specific primers, and satisfying results were obtained. Compared to the standard PCR-SSCP method, this asymmetric PCR-SSCP method has advantages of increased speed, reproducibility, and convenience. Along with PCR-SSP or sequence-based typing, this method will be useful in routine typing of HLA-DRB allele.

• Radiation Oncology Journal
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• v.13 no.2
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• pp.113-120
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• 1995

Purpose : To investigate the effect of alkaloid fraction from Korean ginseng on radiation-induced DNA double strand breaks (dsb) formation and repair in murine lymphocytes Materials and Methods : We used the neutral filter elution technique to assay $^{60}Co\;{\gamma}$ ray-induced DNA double strand breaks formation and repair in C57BL/6 mouse spleen lymphocytes for evaluating the dose-response relationship in the presence of alkaloid fraction as a radioprotective agent. The lymphocytes were stimulated with Phytohemagglutinin (PHA, 2 u g/ml) to label $^3[H]-thymidine.$ Isotope-labelled lymphocytes in suspension were exposed to 100 Gy at $0^{\cdot}C$ in the alkaloid fraction-treated group and elution procedure was performed at PH 9.6. The extents of formation of radiation-induced DNA double strand breaks and repair were compared respectively via strand scission factor (SSF) and relative strand scission factor (RSSF). Results: Alkaloid fraction reduced the formation of double strand breaks with dose modification factor of 2 15, compared to control group Rejoining of DNA dsb appeared to take place via two components. The first fast component was completed within 20.4 minutes, but the second slow component was not completed until 220.2 minutes after irradiation. About $30\%$ of dsb formed by irradiation was ultimately unrejoined despite the administration of alkaloid fraction. The administration of alkaloid fraction had a great effect on the second slow component of repair; the half-time of fast component repair was not changed, but that of slow component was 621.8 minutes. Conclusion: Neutral filter elution assay Proved to be a very effective method to quantitate the extents of DNA dsb formation and its repair. By using this technique, we were able to evaluate the efficiency of alkaloid fraction from Korean ginseng as a valuable radioprotector. Alkaloid fraction can be used prophylactically to prevent or ameliorate the severe radiation damages in workers and neighbors around the atomic power plants. For more refined study, however, more advanced purification of alkaloid fraction wil be needed in the near future.

• Journal of Korean Society of Forest Science
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• v.80 no.2
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• pp.187-192
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• 1991

This study was carried out to estimate the stand volume for Japanese larch(Larix leptolepis) by Strand sampling method. The data collected for this study were based on the 380 sample plots from the field survey, which were distributed in the major part of Korea(Kyeongi, Kangweon, Chungbuk, Chungnam, Chunbuk and Kyeongbuki), and the plotless sampling instrument such as dendrometer, spiegel relascope and tele-relascope were used. The procedure for this study is summarized briefly as follows : 1. There were not only significant differences between volume estimation by Strand sampling method and that by plot survey method, and the relationship was y=bx, where b approached nearly 1. Therefore, the stand volume of Japanese larch could by estimated by Strand sampling method. 2. The value measured by three different plotless sampling instruments did not showed any significant differences between instruments and observers, density and instruments, and ground slope and instruments. 3. With the stand volume, basal area height showed the highest correlation and stand form height, average height, basal area per ha correlated with the volume in thier orders. 4. The best fitted equation of stand volume estimation with basal area height by relascope was as follow. log V=-0.0375+0.8910 log GH-1.5946 1/GH Stand volume table also was obtained using the above estimeated equation. 5. The relationship between estimated value and actual value was Y=bx, where b was nearly 1. The correlation coefficient was very high and the percentage of estimated error was 4.5%.

• 기술발표회
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• s.2006
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• pp.66-72
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• 2006

The ground anchoring has been utilized over 40 years. It is growing the application of the removal ground anchor with tension force for holding earth retaining constructions in the city. It transmits tension stress of prestressed steel wire through grouting to fixed the ground that is of great advantage adjacent ground stability. Nowadays, we can find the compression dispersion anchor on many site. But, it has some problems in behavior of anchors because of impossible to tense p.c strand uniformly under the existing equipment due to different length of p c strand. Hence, motive of this research was to study the application of the newly developed tension system, that analyze and compare with the current anchoring method build on the data of in-site test and laboratory test. As a result, in case of auto back tension system, it became clear that tension pressure was equally distributed among the steal wires but the existing tension system showed sign of instability by indicating stress deflection of about 30% compare with design load. This can cause an ultimate failure of the concentrated p.c strand and a shear failure of ground.

• Structural Monitoring and Maintenance
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• v.2 no.4
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• pp.339-355
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• 2015

Corrosion of prestressed concrete structures is one of the main challenges that engineers face today. In response to this national need, this paper presents the results of a long-term project that aims at developing a structural health monitoring (SHM) technology for the nondestructive evaluation of prestressed structures. In this paper, the use of permanently installed low profile piezoelectric transducers (PZT) is proposed in order to record the acoustic emissions (AE) along the length of the strand. The results of an accelerated corrosion test are presented and k-means clustering is applied via principal component analysis (PCA) of AE features to provide an accurate diagnosis of the strand health. The proposed approach shows good correlation between acoustic emissions features and strand failure. Moreover, a clustering technique for the identification of false alarms is proposed.

• Journal of Radiation Industry
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• v.5 no.3
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• pp.243-247
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• 2011

In this study, a convenient method for the selective immobilization of single strand DNA (ssDNA) on a polymer surface was described. A positively charged polyelectrolyte, poly(diallyldimethylammonium chloride) (PDDA), was spin-coated on a tissue culture petridish and the micropatterns of the PDDA were formed by selective ion implantation through a pattern mask. The surface property of the implanted PDDA was investigated by using a surface profiler and FT-IR spectrometer. Cy3-labeled ssDNA was selectively immobilized on the PDDA patterns through ionic interaction and thus, well-defined ssDNA patterns were obtained.

• Bulletin of the Korean Chemical Society
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• v.27 no.9
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• pp.1346-1352
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• 2006

We describe an effective method of microchip electrophoresis (ME) based on single strand conformation poly-morphism (SSCP) analysis to rapidly detect the point mutation, Leu72Met, in a human obesity gene. The 207-bp dsDNA in the Leu72Met region, an estimate of the child obesity DNA mutant, was amplified by polymerase chain reaction (PCR) and submitted to a conventional glass microchip analysis with a sieving matrix of 1.75% poly(vinylpyrrolidone) (Mr 1 300 000), 1.0% poly(ethyleneoxide) (Mr 600 000) and 5.0% w/w glycerol. When combined with base stacking (BS) with hydroxide ions, the SSCP-ME provided rapid analysis as well as sensitive detection. The detection sensitivity was effectively enhanced in the OH- concentration range of 0.01-0.025 M NaOH. The sensitivity and speed of this ME-based SSCP methodology for the rapid detection of Leu72Met point mutations makes this an attractive method for diagnosing childhood obesity in a clinical diagnostic laboratory.