• Journal of Sensor Science and Technology
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• v.32 no.5
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• pp.295-299
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• 2023

Herein, we propose a simple fabrication method for MXene-coated V-groove sensors for applications. To enhance the sensitivity of this sensor, we applied MXene particles, instead of conventional metal layers, as a sensing material on the sensor's surface. This allows for an easier fabrication, as well as higher sensitivity of the sensor compared to those of our previously demonstrated metal-based V-groove sensor. Additionally, polyurethane-acrylate, a UV-curable liquid polymer, can be easily applied using micro-electromechanical systems-based surface-texture micromachining. The sensor sensitivity is approximately 0.08 /mm, and it can be improved by increasing the number of V-grooves. We believe that the proposed MXene-based wearable sensor offers a great potential in detecting various types of motions characteristic of human activities.

• Journal of the Society of Naval Architects of Korea
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• v.61 no.3
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• pp.200-207
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• 2024

A multi-component force sensor has been developed to measure force and moment components in high-speed flow media for submerged models. The size of the test model is determined based on the Reynolds number of the model at the test speed and expected blockage effect. A two-component force sensor unit has been created by assembling pairs of column elements arranged symmetrically under an eccentric load. The six-component force sensor is constructed with symmetric arrangements of two-component force sensor units in a rectangular plane. The signals generated from the strain gauges attached to the surface of the elements can be converted into force signals. The performance of the waterproof six-component force sensor has been evaluated through calibration. A simplified interference decomposition procedure has been introduced to increase the accuracy of measurement.

• Composites Research
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• v.33 no.4
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• pp.228-233
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• 2020

In this paper, a single lap shear test was performed using a glass fiber reinforced composite material (GFRC). Pencil lead drawn paper sensor (PLDPS) was applied for single lap shear test being performed. Bisphenol-A epoxy and amine hardener were used as adhesives combining with composite materials. To make a difference in adhesive properties, the adhesive was cured under different conditions. PLDPS was made of a 4B pencil on A4 paper. Because graphite in a pencil was an electrically conductive substance, electric resistance (ER) could be measured. A change in ER was observed by a position where a PLDPS was attached to single lap shear specimens. It was confirmed that the change in ER was different depending on two attached positions and was observed by lap shear strain as well. In case the lap shear strain was large, the change in ER of PLDPS was high. This was because the larger the extension of the adhesive part, the larger the degree of bending of the specimen and thus the larger the distance change between two electrodes.

• Korean Journal of Microbiology
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• v.46 no.3
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• pp.240-247
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• 2010

Pseudomonas aeruginosa is a Gram (-) opportunistic human pathogen causing a wide variety of infections on lung, urinary tract, eyes, and burn wound sites and quorum sensing (QS), a cell density-sensing mechanism plays an essential role in Pseudomonas pathogenesis. In order to investigate the importance of QS in the Pseudomonas infections of Korean patients, we isolated 189 clinical strains of P. aeruginosa from the patients in Pusan Paik Hospital, Busan, South Korea. The QS signal production of these clinical isolates was measured by signal diffusion assay on solid media using reporter strains. While most clinical strains (79.4%) produced the QS signals as similar level as a wild type strain, PAO1 did, where LasR, the initial QS signal sensor-regulator was fully activated, a minority of them (4.2%) produced much less QS signals at the level to which LasR failed to respond. Similarly, while 72.5% of the clinical isolates produced QS signals enough to activate QscR, an another QS signal sensor-regulator, some few of them (9%) produced the QS signals at much lower level where QscR was not activated. For further analysis, we selected 74 clinical strains that were obtained from the patients under suspicion of Pseudomonas infection and investigated the total protease activity that is considered important for virulence. Interestingly, significant portion of them showed very low protease activity (44.6%) or no detectable protease activity (12.2%). When the biofilm-forming ability that is considered very important in chronic infection was examined, most isolates showed lower biofilm-forming activity than PAO1. Similarly, significant portion of clinical isolates showed reduced motility (reduced swarming activity in 51.4% and reduced twitching activity in 41.9%), or non-detectable motility (swarming-negative in 28.4% and twitching-negative in 28.4%). Our result showed that the clinical isolates that produced QS signals at the similar level to wild type could have significantly reduced activities in the protease production, biofilm formation, and motility, and some clinical isolates had unique patterns of motility, biofilm formation, and protease production that are not correlated to their QS activity.

• Journal of the Korean Society for Nondestructive Testing
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• v.24 no.5
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• pp.499-507
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• 2004

We have studied tile detection techniques, which can determine the location and the weight of an intruder into infrastructure, by using fiber-optic ROTDR (Rayleigh optical time domain reflectometry) sensor and fiber-optic BOTDA (Brillouin Optical time domain analysis) sensor, which can use an optical fiber longer than that of ROTDR sensor Fiber-optic sensing plates of ROTDR sensor, which arc buried in sand, were prepared to respond the intruder effects. The signal of ROTDR was analyzed to confirm the detection performance. The constructed ROTDR could be used up to 10km at the pulse width of 30ns. The location error was less than 2 m and the weight could be detected as 4 grades, such as 20kgf, 40kgf, 60kgf and 80kgf. Also, fiber optic BOTDA sensor was developed to be able to detect intrusion effect through an optical fiber of tells of kilometers longer than ROTDR sensor. fiber-optic BOTDA sensor was constructed with 1 laser diode and 2 electro-optic modulators. The intrusion detection experiment was peformed by the strain inducing set-up installed on an optical table to simulate all intrusion effect. In the result of this experiment, the intrusion effort was well detected as the distance resolution of 3m through the fiber length of about 4.81km during 1.5 seconds.

• Journal of the Korean Society for Nondestructive Testing
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• v.29 no.4
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• pp.316-322
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• 2009

This paper presents a preliminary study to monitor the lateral behavior of pile foundation using multiplexed fiber Bragg grating(FBG) sensors. In the Preliminary study, an 1.7 meter long cantilever beam with the shape of square hollow box was fabricated and tested under the static loading. Four FBG sensors were multiplexed in a single optical fiber and installed into the top and bottom of the cantilever beam. The strains are directly measured from FBG sensors followed by curvature calculations based on the plane section assumption. Vertical deflections are then estimated using the regression analyses based on the geometric relationships. It has been found that excellent correlation with conventional sensing system was observed. The success of the test encourages the use of the FBG sensing system as a monitoring system for pile foundations. However, further consideration should be given in the case of the sensor malfunction for the practical purpose.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.1
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• pp.23-29
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• 2020

The skin is colonized by a large number of microorganisms with a stable composition of species. However, disease states of skin such as acne vulgaris, psoriasis, and atopic dermatitis have specific microbiome compositions that are different from those of healthy skin. The target modulation of the skin microbiome can be a potential treatment for these skin diseases. Quorum sensing (QS), a bacterial cell-cell communication system, can control the survival of bacteria and increase cell density. Also, QS affects the pathogenicity of bacteria such as biofilm formation and protease production. In this study, we confirmed anti-QS activity of Amorepacific patented ingredients, which are Lactobacillus ferment lysate (using Lactobacillus plantarum APsulloc 331261, KCCM 11179P) through bio-reporter bacterial strain Chromobacterium violaceum. The purple pigment production of C. violaceum controlled by QS was reduced 27.3% by adding 10 ㎍/mL of Lactobacillus ferment lysate (freeze dried). In addition, the Lactobacillus ferment lysate increased growth of Staphylococcus epidermidis 12% and decreased growth of Pseudomonas aeruginosa 38.5% and its biofilm formation 17.7% at a concentration of 10 ㎍/mL compared to the untreated control group. Moreover, S. epidermidis was co-cultured with the representative dermatological bacterium Staphylococcus aureus in the same genus, the growth of S. epidermidis was increased 134 % and the growth of S. aureus was decreased 13%. These results suggest that fermented lysate using Lactobacillus plantarum APsulloc 331261 may be useful as a cosmetic ingredient that can control the balance of skin microbiome.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.5
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• pp.507-512
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• 2005

This research focuses on the development and application of a method for the detection of m-toluate in soils using a genetically engineered bioluminescent bacteria, Pseudomonas putida mt-2 KG1206. KG1206 produces light by direct (m-toluate and benzoate) and indirect (toluene analogs) inducers. For detection of m-toluate in soil system, 9.9 mL strain was amended with 0.1 mL soil ethanol extractant. A high correlation ($r^2>0.97$) was observed between bioluminescence and m-toluate concentration. The unknown concentrations of m-toluate in soil samples were pre-determined using a method developed based on bioluminescence activity of strain with extracted inducers. Values between by LC analysis and bioluminescence activity show moderate statistical results. These results demonstrate the feasibility of recombinant bioluminescent microorganism, engineered to generate a quantifiable bioluminescence signal in response to specific pollutants, may serve as combined sensing and reporting tools in environmental monitoring.

• Smart Structures and Systems
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• v.15 no.2
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• pp.245-258
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• 2015

A methodology based on distributed fiber optic sensors is proposed to detect the lateral buckling for subsea pipelines in this study. Uncontrolled buckling may lead to serious consequences for the structural integrity of a pipeline. A simple solution to this problem is to control the formation of lateral buckles among the pipeline. This firms the importance of monitoring the occurrence and evolution of pipeline buckling during the installation stage and long-term service cycle. This study reports the experimental investigations on a method for distributed detection of lateral buckling in subsea pipelines with Brillouin fiber optic sensor. The sensing scheme possesses the capability for monitoring the pipeline over the entire structure. The longitudinal strains are monitored by mounting the Brillouin optical time domain analysis (BOTDA) distributed sensors on the outer surface of the pipeline. Then the bending-induced strain is extracted to detect the occurrence and evolution of lateral buckling. Feasibility of the method was validated by using an experimental program on a small scale model pipe. The results demonstrate that the proposed approach is able to detect, in a distributed manner, the onset and progress of lateral buckling in pipelines. The methodology developed in this study provides a promising tool for assessing the structural integrity of subsea pipelines.

• Journal of Microbiology and Biotechnology
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• v.30 no.2
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• pp.187-195
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• 2020

To understand the molecular mechanism involved in the survivability of cold-tolerant lactic acid bacteria was of great significance in food processing, since these bacteria play a key role in a variety of low-temperature fermented foods. In this study, the cold-stress response of probiotic Lactobacillus plantarum K25 isolated from Tibetan kefir grains was analyzed by iTRAQ proteomic method. By comparing differentially expressed (DE) protein profiles of the strain incubated at 10℃ and 37℃, 506 DE proteins were identified. The DE proteins involved in carbohydrate, amino acid and fatty acid biosynthesis and metabolism were significantly down-regulated, leading to a specific energy conservation survival mode. The DE proteins related to DNA repair, transcription and translation were up-regulated, implicating change of gene expression and more protein biosynthesis needed in response to cold stress. In addition, two-component system, quorum sensing and ABC (ATP-binding cassette) transporters also participated in cell cold-adaptation process. These findings provide novel insight into the cold-resistance mechanism in L. plantarum with potential application in low temperature fermented or preserved foods.