Browse > Article

Detection of m-toluate in Soils using Bioluminescence Producing Recombinant Bacteria  

Kong, In-Chul (School of Civil, Urban and Environmental Engineering, Yeungnam University)
Kim, Myung-Hee (School of Civil, Urban and Environmental Engineering, Yeungnam University)
Jung, Yun-Ho (School of Civil, Urban and Environmental Engineering, Yeungnam University)
Ko, Kyung-Seok (Geo-Environmental Division, Korea Institute of Geoscience and Mineral Resources)
Kim, Jae-Gon (Geo-Environmental Division, Korea Institute of Geoscience and Mineral Resources)
Shin, Sung-Chun (Geo-Environmental Division, Korea Institute of Geoscience and Mineral Resources)
Publication Information
Journal of Korean Society of Environmental Engineers / v.27, no.5, 2005 , pp. 507-512 More about this Journal
Abstract
This research focuses on the development and application of a method for the detection of m-toluate in soils using a genetically engineered bioluminescent bacteria, Pseudomonas putida mt-2 KG1206. KG1206 produces light by direct (m-toluate and benzoate) and indirect (toluene analogs) inducers. For detection of m-toluate in soil system, 9.9 mL strain was amended with 0.1 mL soil ethanol extractant. A high correlation ($r^2>0.97$) was observed between bioluminescence and m-toluate concentration. The unknown concentrations of m-toluate in soil samples were pre-determined using a method developed based on bioluminescence activity of strain with extracted inducers. Values between by LC analysis and bioluminescence activity show moderate statistical results. These results demonstrate the feasibility of recombinant bioluminescent microorganism, engineered to generate a quantifiable bioluminescence signal in response to specific pollutants, may serve as combined sensing and reporting tools in environmental monitoring.
Keywords
Genetically Engineered Microorganism(GEM); Bioluminescence; Toluene Analogs; TOL Plasmid;
Citations & Related Records
연도 인용수 순위
  • Reference
1 King, J. M. H., Digrazia, P. M., Burlage, B., Sanseverino, J., Dunbar, P., Larimer, F., and Sayler, G. S., 'Rapid, sensitive bioluminescent reporter technology for naphthalene exposure and biodegradation,' Science, 249, 778-781(1990)   DOI   PUBMED   ScienceOn
2 Assinder, S. J. and Willams, P. A., 'The TOL Plasmid : Determinants of the catabolism of toluene and the xylenes,' Adv. Microbiol. Phys., 31, 2 - 69(1990)
3 Kohler, S., Belkin, S., and Schmid, R. D., 'Reporter gene bioassays in environmental analysis.' Fresenius J. Anal. Chem., 366, 769-779(2000)   DOI   ScienceOn
4 Inouye, S., Nakazawa, A., and Nakazawa T., 'Overproduction of the xylS gene product and activation of the xylDLEGF operon on the TOL Plasmid,' J. Bacteriol., 169, 3587 - 3592(1987)   DOI   PUBMED
5 Gu, M. B. and Choi, S. H., 'Toxicity biomonitoring of degradation byproducts using freeze-dried recombinant bioluminescent bacteria,' Anal. Chim. Acta, 481, 229-238(2003)   DOI   ScienceOn
6 Chatterjee, J. and Meighen, E. A., 'Biotechnological application of bacterial bioluminescenec (lux) genes,' Photochem. Photobiol., 62(4), 641-650(1995)   DOI   ScienceOn
7 Gu, M. B. and Chang, S. T., 'Soil biosensor for the detection of P AH toxicity using an immobilized recombinant bacterium and a biosurfactant,' Biosens. Biotechnol., 16, 667-674(2001)   DOI   ScienceOn
8 Steinberg, S. M. and Poziomek, E. J., 'A review of environmental application of bioluminescence measurements,' Chemosphere, 30(11), 2155 - 2197(1995)   DOI   ScienceOn
9 Holtel, A., Marques, S., Mohler, I., Jakubzik, U., and Timmis, K. N., 'Carbon source - dependent inhibition of xyl operon expression of the Pseudomonas putida TOL Plasmid,' J. Bacteriol., 176(6), 1773-1776(1994)   DOI   PUBMED
10 Nealson, K. H. and Hastings, J. W., 'Bacterial bioluminescence: Its control and ecological significance,' Microbiol. Rev., December, 496-518(1979)   PUBMED
11 Kong, I, C., Suh, H., Yang, Z., and Burlage, R. S., 'A bioluminescence reporter strain utilizing the lower pathway promoter (Pm) of the xyl operon of Psedumonas; optimization of a bioassay for m-toluate,' Adv. Environ. Res., 8, 647 - 654(2004)   DOI   ScienceOn
12 Harayama, S. and Rekik, M., 'The meta cleavage operon of TOL degradative plasmid pWWO comprises 13 genes,' Mol. Gen. Genet., 221, 113 -120(1990)   DOI   ScienceOn
13 Willardson, B. M., Wilkins, J. F., Rand, T. A., Schupp, J. M., Hill, K. K., Keim, P., and Jackson, P. J., 'Development and testing of a bacterial biosensor for toluene - based environmental contaminants,' Appl. Environ. Microbiol., 64(3), 1006-1012(1998)   PUBMED
14 신창섭, 김기환, 원정일, 'PCB 제조공정에서 발생하는 VOC를 처리하기 위한 흡착제의 흡착특성,' 한국대기환경학회지, 17(1), 67-74(2001)
15 Thouand, G., Horry, H., Durand, M. J., Picart, P., Bendriaa, L., Daniel, P., and Dubow, M. S., 'Detection of bacteria in the food industry with bioluminescence sensors,' Recent Res. Develop. Microbiol., 5, 79-93(2001)
16 Burlage. R. S., Palumbo, A. V., Heitzer, A., and Sayler, G. S., 'Bioluminescent reporter bacteria detect contaminants in soil samples,' Appl. Biochem. Biotech., 45, 731-740(1994)   DOI   ScienceOn