• Journal of Microbiology and Biotechnology
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• v.18 no.2
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• pp.219-227
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• 2008

The free-living photoheterotrophic Gram-negative bacterium Rhodobacter sphaeroides possesses a quorum-sensing (QS) regulatory system mediated by CerR-CerI, a member of the LuxR-LuxI family. To identify the genes affected by the regulatory system, random lacZ fusions were generated in the genome of R. sphaeroides strain 2.4.1 using a promoter-trapping vector, pSG2. About 20,000 clones were screened and 23 showed a significantly different level of ${\beta}$-gal activities upon the addition of synthetic 7,8-cis-N-tetradecenoyl-homoserine lactone (RAI). Among these 23 clones, the clone showing the highest level of induction was selected for further study, where about a ten-fold increase of ${\beta}$-gal activity was exhibited in the presence of RAI and induction was shown to be required for cerR. In this clone, the lacZ reporter was inserted in a putative gene that exhibited a low homology with catD. A genetic analysis showed that the expression of the catD homolog was initiated from a promoter of another gene present upstream of the catD. This upstream gene showed a strong homology with luxR and hence was named qsrR (quorum-sensing regulation regulator). A comparison of the total protein expression profiles for the wild-type cells and qsrR-null mutant cells using two-dimensional gel electrophoresis and a MALDI-TOF analysis allowed the identification of sets of genes modulated by the luxR homolog.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.271-280
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• 2010

Vibrio alginolyticus, a Gram-negative marine bacterium, is one of the causative agents of fish vibriosis. Its virulence factors and pathogenesis mechanism are barely known, except for some extracellular products (ECPs) that are known to be regulated by quorum sensing system. Therefore, the present study used a microarray to analyze the transcription profiles of the wild-type V. alginolyticus and a deletion mutant of luxO, the pivotal regulator in Vibrio quorum sensing systems, which resulted in the identification of a putative virulence factor, MviN. Quantitative real-time reverse transcription PCR confirmed that the transcription of mviN was upregulated in the luxO mutant when compared with wild-type, and down regulated in a luxO-con complemented strain. Furthermore, Western blotting indicated that MviN was greatly induced during the late-exponential and stationary phases of growth, indicating that the expression of MviN was cell-density dependent and quorum sensing regulated in V. alginolyticus. Meanwhile, the mviN null mutant displayed a much slower growth rate than the wild type, signifying the essential role of MviN in V. alginolyticus. Western blotting also revealed that MviN was present as an extracellular protein in V. alginolyticus. When epithelioma papulosum cyprini (EPC) cells were treated with the ECPs of the mviN mutant, no cytotoxicity was observed, whereas EPC cells treated with the wild type exhibited pathological changes, which increased with the ECPs concentration and treatment time. Therefore, the results demonstrated that MviN is a LuxO-regulated ECPs component and involved in the pathogenicity of V. alginolyticus.

• Composites Research
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• v.23 no.2
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• pp.24-30
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• 2010

Interfacial evaluation and hydrophobicity of Ni-nanopowder/epoxy composites were investigated for self-sensing and actuation. Contact resistance and resistivity were measured using gradient micro-specimens. The actuation of the composites in the electromagnetic field was studied with three wave functions, i.e., sine, triangle and square functions. Due tothe presence of hydrophobic domains on the heterogeneous surface, the static contact angle of Ni-nanopowder/epoxy nanocomposite wasabout $100^{\circ}$, which was rather lower than that for super-hydrophobicity. The dynamic contact angle showed the similar trend of static contact angle. Ni-nanopowder/epoxy composite was responded wellfor both self-sensing and actuation in electromagnetic field due to the intrinsic metal property of Ni-nanopowder. Displacement of the actuator of Ni-nanopowder/epoxy composite was evaluated to obtain the maximum and the optimum performance using laser displacement sensor as functions of the wave type, frequency, and voltage. Actuation of Ni-nanopowder/epoxy composites also increased as functions of applied frequency and voltage. Actuated strain increased more rapidly at sine wave with increasing voltage compared to those of triangle or rectangular waves.

• Journal of the Korean Society for Precision Engineering
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• v.18 no.2
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• pp.214-221
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• 2001

This paper describes the design of 3-component force/moment sensor with the force and moment ratio of wide range. It can measure the x-direction force Fx, y-direction force Fy and z-direction moment Mz simultaneously. In order to accurately measure forces and moment using 3-component force/moment sensor, it should get suitable force and moment ratio(the ratio of force Fx=200 N and moment Mz=20 Nm is ten to one), and small interference error. In this paper, in order to design the 3-component force/moment sensor with the force and moment ratio of wide range, the procedures are performed as follow : 1) the derivation of the equations to predict the bending strains on the surfaces of the plate-beams under the force or the moments, 2) the determination of the size of the sensing elements of the force/moment sensor by using the derived equations, 3) the Finite Element Method(FEM) analysis and the characteristic test for confirming the strains from the theory analysis, 4) the selection of the attachment locations of the strain gages of each sensor, 5) the analysis of the rated strain and the interference error at the attachment location of strain gages. It reveals that the rated strains calculated from the derived equations make a good agreement with the results from the Finite Element Method analysis and the characteristic test.

• Journal of the Korean Society of Hazard Mitigation
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• v.6 no.3 s.22
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• pp.9-16
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• 2006

In structural health monitoring, the safety of structural members are assessed by the level of stress measured by various strain sensors based on different sensing mechanisms. Since most existing strain sensors used for health monitoring system can cover a relatively small range of structural members, it is very difficult to measure the maximum value of the member subjected to varying amount and types of loads with those point sensors. The reliability of assessed safety of a member may be improved by increasing the number of sensors. It may not be also realistic to increase the number of sensors to overcome these drawbacks. In this paper, a stress measuring method for beam-column members is developed by estimating the maximum stress based on the average strains obtained from long gauge sensor. The average strain from long gage fiber optic sensor is transformed into the maximum strain by multiplication of the modification factor derived in this research.

• Smart Structures and Systems
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• v.6 no.5_6
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• pp.641-659
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• 2010

Structural Health Monitoring (SHM) gradually becomes a technique for ensuring the health and safety of civil infrastructures and is also an important approach for the research of the damage accumulation and disaster evolving characteristics of civil infrastructures. It is attracting prodigious research interests and the active development interests of scientists and engineers because a great number of civil infrastructures are planned and built every year in mainland China. In a SHM system the sheer number of accompanying wires, fiber optic cables, and other physical transmission medium is usually prohibitive, particularly for such structures as offshore platforms and long-span structures. Fortunately, with recent advances in technologies in sensing, wireless communication, and micro electro mechanical systems (MEMS), wireless sensor technique has been developing rapidly and is being used gradually in the SHM of civil engineering structures. In this paper, some recent advances in the research, development, and implementation of wireless sensors for the SHM of civil infrastructures in mainland China, especially in Dalian University of Technology (DUT) and Harbin Institute of Technology (HIT), are introduced. Firstly, a kind of wireless digital acceleration sensors for structural global monitoring is designed and validated in an offshore structure model. Secondly, wireless inclination sensor systems based on Frequency-hopping techniques are developed and applied successfully to swing monitoring of large-scale hook structures. Thirdly, wireless acquisition systems integrating with different sensing materials, such as Polyvinylidene Fluoride(PVDF), strain gauge, piezoresistive stress/strain sensors fabricated by using the nickel powder-filled cement-based composite, are proposed for structural local monitoring, and validating the characteristics of the above materials. Finally, solutions to the key problem of finite energy for wireless sensors networks are discussed, with future works also being introduced, for example, the wireless sensor networks powered by corrosion signal for corrosion monitoring and rapid diagnosis for large structures.

• Journal of the Korean Society for Marine Environment & Energy
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• v.14 no.4
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• pp.280-286
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• 2011

In this research, the oil detection method and the characteristic of sensor using the selective reaction of silicone rubber in response to hydrocarbon oil will be described. As a sensing principle, the swelling property of silicone rubber in response to hydrocarbon fuel is used, also a strain gauge is used to transduce the volume change to an electrical signal. The sensor core is manufactured with a strain gauge embedded in silicone rubber by the curing process and experiments for characteristics of sensor core with various oils were carried out. It is shown that the sensor core can be used as an oil spill detection sensor. Also, for the application to the sea area, a buoy type sensor platform is integrated with a sensor core and a strain amplifier and it is tested in the simulated oil spill condition. In this study, it is proven that the integrated sensor can be used for the detection of various oils.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.42 no.2
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• pp.151-162
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• 2022

Prefab members have attracted attention because they can be mass-produced in factories through smart construction technology. For prefab prestressed concrete girders, it is important to manage the shapes of the girders properly from production to the pre-installation stage for consistency with the prefab floor plate during the erection process. This paper presents a camber reconstruction method using collocated strain measurements from the top and bottom of the prefab girder. In particular, the camber reconstruction method is applied to measured strain data in which the time-dependent behavior of concrete is considered after the introduction of prestress. Through Monte Carlo numerical simulations, the statistical accuracy of the reconstructed camber for a limited number of sensors, measurement errors, and nonlinear time-dependent behaviors are analyzed and validated.

• Science of Emotion and Sensibility
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• v.21 no.1
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• pp.83-90
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• 2018

The purpose of this study is for fundamental research of meditation smart wear for physical and mental healing, and researching method for monitoring phase of meditation through textile by measuring the number of abdominal respiration when meditating. For this purpose, the research implemented Single Wall Carbon Nano-Tube (SWCNT) based strain gauges type textile sensor, considered reliability and validity of respiratory sensing, and analyzed efficiency of respiratory sensing based on body parts comparatively. The first preliminary experiment was to evaluate the performance of textile sensor through abdominal model dummy which open and shut of 5 cm repeatedly for 2 minutes at the rate of 0.1Hz in order to simulate abdominal respiration. It concluded signal efficiency between reference sensor(BIOPAC) and textile respiratory sensor appears statistically significant (p<0.001). The second experiment were conducted with 4 subjects doing abdominal respiration under same conditions, and after comparing the signal values between two sensors from 4 attached locations(around center and sides of omphali and phren), center of omphali and sides of phren were selected as suitable location for measuring meditational breathing as they showed large and stable signals. In result, this research aimed for implementing of the textile sensor for sensing meditational breathing of long respiration cycle, review of reliability and validity for sensing number of meditational respiration with the sensor and consideration of sensing efficiency by sensing location on body parts.

• International Journal of Control, Automation, and Systems
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• v.5 no.4
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• pp.419-428
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• 2007

This paper describes the development of a six-axis force/moment sensor with rectangular taper beams for an intelligent robot's wrist and ankle. In order to accurately push and pull an object with an intelligent robot's hand, and in order to safely walk with an intelligent robot's foot, the robot's wrist and ankle should measure three forces Fx, Fy, and Fz, and three moments Mx, My, and Mz simultaneously from the mounted six-axis force/moment sensor to the intelligent robot's wrist and ankle. Unfortunately, the developed six-axis force/moment sensor utilized in other industrial fields is not proper for an intelligent robot's wrist and ankle in the size and the rated output of the six-axis force/moment sensor. In this paper, the structure of a six-axis force/moment sensor with rectangular taper beams was newly modeled for an intelligent robot's wrist and ankle, and the sensing elements were designed by using the derived equations, following which the six-axis force/moment sensor was fabricated by attaching strain-gages on the sensing elements. Moreover, the characteristic test of the developed sensor was carried out by using the six-component force/moment sensor testing machine. The rated outputs from the derived equations agree well with those from the experiments. The interference error of the sensor is less than 2.87%.