• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2004.05a
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• pp.174-177
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• 2004

The nickel-based alloy Nimonic 80A possesses strength, and corrosion, creep and oxidation resistance at high temperature. These products are used for aerospace, marine engineering and power generation, etc. The control of forging parameters such as strain, strain rate, temperature and holding time is important because the microstructure change in hot working affects the mechanical properties. It is necessary to understand the microstructure variation evolution. The microstructure change evolution occurs by recovery, recrystallization and grain growth phenomena. The dynamic recrystallization evolution has been studied in the temperature range $950-1250^{\circ}C$ and strain rate range $0.05-5s^{-1}$ using hot compression tests. The metadynamic recrystallization and grain growth evolution has been studied in the temperature range $950-1250^{\circ}C$ and strain rate range 0.05, $5s^{-1}$, holding time range 5, 10, 100, 600 sec using hot compression tests. Modeling equations are developed to represent the flow curve, recrystallized grain size, recrystallized fraction and grain growth phenomena by various tests. Parameters of modeling equation are expressed as a function of the Zener-Hollomon parameter. The modeling equation for grain growth is expressed as a function of initial grain size and holding time.

• Korean Chemical Engineering Research
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• v.47 no.5
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• pp.620-623
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• 2009

Flavobacterium sp. strain DS5(NRRL B-14859) was used to convert oleic acid to 10-ketostearic acid(10-KSA) via 10-hydroxystearic acid(10-HSA). Increase in cell concentration by centrifuging, collecting cells grown in two flasks, and resuspending in one flask, improved 10-KSA production to 6.5 g/L from 3.5 g/L in a usual flask culture. Tween-80 addition to the culture did not greatly affect the production of 10-KSA and 10-HSA. When culture broth was centrifuged after fermentation, it was observed that pellets were separated into two parts(yellow and white). Gas chromatography analysis showed that 10-KSA and 10-HSA were detected only in a white pellet, suggesting that the bioconversion products of strain DS5 are extracellularly produced and can be easily recovered from cells by a simple centrifugation step.

• Molecules and Cells
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• v.28 no.6
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• pp.575-581
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• 2009

Ornithine decarboxylase (ODC) is a rate-limiting enzyme in the biosynthesis of polyamines, which are essential for cell growth, differentiation, and proliferation. This report presents the characterization of an ODC-encoding cDNA (SlitODC) isolated from a moth species, the tobacco cutworm, Spodoptera litura (Lepidoptera); its expression in a polyamine-deficient strain of yeast, S. cerevisiae; and the recovery in polyamine levels and proliferation rate with the introduction of the insect enzyme. SlitODC encodes 448 amino acid residues, 4 amino acids longer than B. mori ODC that has 71% identity, and has a longer C-terminus, consistent with B. mori ODC, than the reported dipteran enzymes. The null mutant yeast strain in the ODC gene, SPE1, showed remarkably depleted polyamine levels; in putrescine, spermidine, and spermine, the levels were > 7, > 1, and > 4%, respectively, of the levels in the wild-type strain. This consequently caused a significant arrest in cell proliferation of > 4% of the wild-type strain in polyamine-free media. The transformed strain, with the substituted SlitODC for the deleted endogenous ODC, grew and proliferated rapidly at even a higher rate than the wild-type strain. Furthermore, its polyamine content was significantly higher than even that in the wild-type strain as well as the spe1-null mutant, particularly with a very continuously enhanced putrescine level, reflecting no inhibition mechanism operating in the putrescine synthesis step by any corresponding insect ODC antizymes to SlitODC in this yeast system.

• Journal of Microbiology and Biotechnology
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• v.34 no.2
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• pp.407-414
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• 2024

Phosphorus is an essential but non-renewable nutrient resource critical for agriculture. Luxury phosphorus uptake allows microalgae to synthesize polyphosphate and accumulate phosphorus, but, depending on the strain of algae, polyphosphate may be degraded within 4 hours of accumulation. We studied the recovery of phosphorus from wastewater through luxury uptake by an engineered strain of Synechocystis sp. with inhibited polyphosphate degradation and the effect of this engineered Synechocystis biomass on lettuce growth. First, a strain (∆phoU) lacking the phoU gene, which encodes a negative regulator of environmental phosphate concentrations, was generated to inhibit polyphosphate degradation in cells. Polyphosphate concentrations in the phoU knock-out strain were maintained for 24 h and then decreased slowly. In contrast, polyphosphate concentrations in the wild-type strain increased up to 4 h and then decreased rapidly. In addition, polyphosphate concentration in the phoU knockout strain cultured in semi-permeable membrane bioreactors with artificial wastewater medium was 2.5 times higher than that in the wild type and decreased to only 16% after 48 h. The biomass of lettuce treated with the phoU knockout strain (0.157 mg P/m²) was 38% higher than that of the lettuce treated with the control group. These results indicate that treating lettuce with this microalgal biomass can be beneficial to crop growth. These results suggest that the use of polyphosphate-accumulating microalgae as biofertilizers may alleviate the effects of a diminishing phosphorous supply. These findings can be used as a basis for additional genetic engineering to increase intracellular polyphosphate levels.

• Journal of Biomedical Engineering Research
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• v.10 no.3
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• pp.261-268
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• 1989

The characteristics of aging of near-equiatomic Ni-Ti alloy has been studied by the three point bend test and the measurement of Differential Scanning Calorimetry(DSC). The DSC is used to measure precise transformation temperatures and the amount of thermal energy required for the corresponding phase transformation. The effort of hardness on aging treatment in saline solution of $37^{\circ}C$ was higher for the annealed than solution treated specimens. As the testing temperature inc- rease from under $M_f$ to above $A_f$ temperature, the elastic stiffness increased. Almost full recovery can be achieved after bending below Belo outer fiber strain. Total bend recovery decreased gradually as aging time and bend angle is increased.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2006.05a
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• pp.159-163
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• 2006

The effects of annealing temperature and time on mechanical properties and microstructures were studied in cold drawn pearlitic steel wires containing 0.84wt% Si. Annealing was performed from $200^{\circ}C$ to $450^{\circ}C$ with different time of 30sec, 1min, 15min and 1hr. The increase of tensile strength at low temperature was related with strain ageing. The decrease of tensile strength at high annealing temperature was related with spherodization of cementite and the occurrence of recovery of the lamellar ferrite in the pearlite. The improvement of ductility was connected with spherodization of cementite plate in pearlite and recovery process by reduction of high dislocation density at short time annealing temperature of $400^{\circ}C$.

• Interaction and multiscale mechanics
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• v.2 no.3
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• pp.277-293
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• 2009

The Enriched Free Mesh Method (EFMM) is a patch-wise procedure in which both a displacement field on an element and a stress/strain field on a cluster of elements connected to a node can be defined. On the other hand, the Superconvergent Patch Recovery (SPR) is known to be an efficient post-processing procedure of the finite element method to estimate the error norm at a node. In this paper, we discuss the relationship between solutions of the EFMM and those of the SPR through several convergence studies. In addition, in order to solve the demerit of the smoothing effect on the fracture mechanics fields, we implement a singular stress field to a local patch in the EFMM, and its effectiveness is investigated.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2006.09b
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• pp.718-719
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• 2006

Two different commercial aluminium powder grades have been densified by direct hot extrusion. The extrusion temperature was $425^{\circ}C$, with an extrusion ratio of 1:16. Prior to extrusion, some green compacts were pre-sintered ($500^{\circ}C$). The evolution of the extrusion load during the process and the hardness of the final products have been investigated. Additionally, microstructural characterization by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM) and Electron Backscattered Diffraction (EBSD) was carried out. The obtained results evidence grain refinement. Additionally, inter-metallic precipitation, dynamic recovery and geometric dynamic recrystallization take place depending on some process variables, powder composition, heat treatment, strain $\ldots$

• Journal of the Korean Society for Heat Treatment
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• v.30 no.6
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• pp.251-257
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• 2017

The effect of annealing temperature on the martensitic transformation behavior, tensile deformation chracteristics and shape recovery etc., has been studied in TiNi based shape memory ribbon fabricated by coldrolling of wire. TiNi based shape memory wire (${\phi}=500{\mu}m$) of which structure is intermetallic compound could be cold-rolled without process annealing up to the reduction rate in thickness of 50%, but a few cracks appear in cold-rolled ribbon in the reduction rate in thickness of 65%. The $B2{\rightarrow}R{\rightarrow}B19^{\prime}$ martensitic transformation or $B2{\rightarrow}B19^{\prime}$ martensitic transformation occurs in annealing conditions dissipating lattice defects introduced by coldrolling. However, in case of higher reduction rate or lower annealing temperature, martensitic transformation in cold-rolled and then annealed ribbons does not occur. The maximum shape recovery rate of cold-rolled ribbons with the reduction rate of 35 and 65% could be achieved at annealing temperatures of 250 and $350^{\circ}C$, respectively. The shape recovery rate seems to be related to the stress level of plateau region on stress-strain curve.

• Journal of Korean Association for Spatial Structures
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• v.14 no.3
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• pp.93-100
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• 2014

Ethylene Tetrafluoroethylene (ETFE) has been widely used in long-span buildings because of its light weight and high transparency. This paper studies the short and long term creep behaviour of ETFE foil. A series of short-term creep and recovery tests were performed, in which the residual strain was observed. A long-term creep test of the ETFE foil was also performed over 110 days. A viscoelastic-plastic model was then established to describe the short-term creep and recovery behaviour. The model contains a traditional multi-Kelvin part and an added steady-flow component to represent the viscoelastic and viscoplastic behaviour, respectively. The model successfully fit the data for three stresses and six temperatures. Additionally, time-temperature equivalency was adopted to predict the long-term creep behaviour of ETFE foil. Horizontal shifting factors were determined from the process of shifting creep-curves at six temperatures. The long-term creep behaviours at three temperatures were predicted. Finally, the long-term creep test showed that the short-term creep test at identical temperatures insufficiently predicted additional creep behaviour, and the long-term test verified the horizontal shifting factors derived from the time-temperature equivalency.