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Production and Recovery of Oxygenated Fatty Acids from Oleic Acid by Flavobacterium sp. Strain DS5  

Heo, Shin-Haeng (Department of Chemical Engineering, College of Engineering, Chungbuk National University)
Kim, Beom Soo (Department of Chemical Engineering, College of Engineering, Chungbuk National University)
Publication Information
Korean Chemical Engineering Research / v.47, no.5, 2009 , pp. 620-623 More about this Journal
Abstract
Flavobacterium sp. strain DS5(NRRL B-14859) was used to convert oleic acid to 10-ketostearic acid(10-KSA) via 10-hydroxystearic acid(10-HSA). Increase in cell concentration by centrifuging, collecting cells grown in two flasks, and resuspending in one flask, improved 10-KSA production to 6.5 g/L from 3.5 g/L in a usual flask culture. Tween-80 addition to the culture did not greatly affect the production of 10-KSA and 10-HSA. When culture broth was centrifuged after fermentation, it was observed that pellets were separated into two parts(yellow and white). Gas chromatography analysis showed that 10-KSA and 10-HSA were detected only in a white pellet, suggesting that the bioconversion products of strain DS5 are extracellularly produced and can be easily recovered from cells by a simple centrifugation step.
Keywords
Oxygenated Fatty Acids; Flavobacterium sp. Strain DS5; 10-Ketostearic Acid; 10-Hydroxystearic Acid; Oleic Acid;
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Times Cited By KSCI : 1  (Citation Analysis)
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