• Title/Summary/Keyword: Strain of Structure

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Transferable R plasmid of Streptococci Ioslation from Diseased Olive Flounder (Paralichthys olivaceus) in Jeju (제주도 양식넙치병어에서 분리된 연쇄상구균의 약제내성 전이성 plasmid)

  • Kim, Jong-Hun;Lee, Chang-Hoon;Kim, Eun-Heui
    • Journal of fish pathology
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    • v.19 no.3
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    • pp.267-276
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    • 2006
  • Seventy-five streptococci were isolated from diseased olive flounder, Paralichthys olivaceus in Jeju. Their drug susceptibility and transferable multiple drug resistance were characterized. All isolates were resistant to flumequine (AR) and oxolinic acid (OA) and 26 isolates (34.7%) showed 4~6 multiple resistance of ampicillin (ABPC), AR, doxycycline (DOXY), erythromycin (EM), norfloxacin(NOR), OA and oxytetracycline (OTC) in various combinations. pST9 of a transferable R plasmid was detected from a multiple drug resistance strain, Streptococcus sp., ST9 originated from diseased flounder in Jeju, previously. We performed DNA hybridization to know the distribution of plasmid with the same DNA structure as pST9 in streptococci. Thirteen out of 60 isolates analyzed were positive in colony DNA hybridization and the part of bacteria isolated from raw meal was also hybridized with pST9. It suggested that raw meal is one of the origin of the resistance plasmid and R plasmid with DNA structure differing from pST9 is also involving in multiple drug resistance of the streptococci. In conjugation experiment, we found transferable R plasmid carrying OTC, DOXY and/or EM resistance determinant in the 13 resistance strains. all of the streptococci carrying the transferable R plasmid were similar in RAPD patterns. However, pST -type R plasmid was rare in S. iniae most frequently appearing in flounder farm.

Effects of Solvent on the Fabrication of Poly(L-lactide) Scaffold Membranes through Phase Inversion (상전이를 통한 Poly(L-lactide) 스캐폴드 막의 제조에서의 용매의 효과)

  • Cho, Yu Song;Kim, Young Kyoung;Koo, Ja-Kyung;Park, Jong Soon
    • Membrane Journal
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    • v.24 no.2
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    • pp.113-122
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    • 2014
  • Porous poly(L-lactic acid)(PLLA) scaffold membranes were prepared via. phase separation process. Chloroform, dichloromethane and 1,4-dioxane were used as solvent and, ethyl alcohol was used as non-solvent. Morphologies, mechanical properties and mass transfer characteristics of the scaffold membranes were investigated through SEM, stress-strain test and glucose diffusion test. The scaffold membranes obtained from the casting solutions with chloroform and with dichloromethane showed similar morphologies. They showed sponge-like porous structure with the pore size in the range of $3-10{\mu}m$ and, their porosities were in 50-80% range. Using 1,4-dioxane as solvent, nano-fibrous scaffold membranes with porosities over 80% were fabricated. When the polymer content in the solution with 1,4-dioxane was lowered to 4%, highly porous, macroporous and nano-fibrous scaffold membranes were obtained. The size of the macropore was tens of the microns and the porosity was around 90%. These results indicate that the solvent has significant effect on the scaffold membrane structure and, that scaffold membranes with various structures can be fabricated through phase separation method by choosing solvent and by controlling polymer concentration in the casting solution.

Damage Analysis of Nearby Structures with the Consideration of Tunnel Construction Conditions in Sandy and Clayey Ground (모래 및 점토지반에서 터널시공조건을 고려한 인접구조물의 손상도 분석)

  • Son, Moorak;Yun, Jongcheol
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.31 no.1C
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    • pp.53-63
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    • 2011
  • This paper investigates the effects of tunnelling-induced ground movements on nearby structures, considering soil-structure interactions of different ground (loose sand, dense sand, soft clay, stiff clay) and construction conditions (ground loss). The response of four-story block structures, which are subjected to tunnelling-induced ground movements, has been investigated in different ground and construction conditions (ground loss) using numerical analysis. The structures for numerical analysis has been modelled using Discrete Element Method (DEM) to have real cracks when the shear and tensile stress exceed the maximum shear and tensile strength. The response of four-story block structures has been investigated with a ground movement magnitude and compared in terms of ground and construction conditions (ground loss) considering the magnitude of deformations and cracks in structures. In addition, the damage levels, which are possibly induced in structures, has been provided in terms of ground and construction conditions (ground loss) using the state of strain damage estimation criterion (Son and Cording, 2005). The results of this study will provide a background for better understandings for controlling and minimizing building damage on nearby structures due to tunnelling-induced ground movements.

The 33-mode Dielectric and Piezoelectric Properties of PIN-PMN-PT Single Crystal under Stress and Electric Field (압축하중 및 전계 인가에 따른 PIN-PMN-PT 단결정의 33-모드 유전 및 압전특성)

  • Lim, Jae Gwang;Park, Jae Hwan;Lee, Jeongho;Lee, Sang Goo
    • Journal of the Microelectronics and Packaging Society
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    • v.27 no.4
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    • pp.91-96
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    • 2020
  • The 33-mode dielectric and piezoelectric properties of Pb(In1/2Nb1/2)O3-Pb(Mg1/3Nb2/3)O3-PbTiO3 piezoelectric single crystals were measured under large electric field and compressive stress. The phase transition from the low temperature rhombohedral to the high temperature tetragonal structure was observed in the range of 110~140℃, and the Curie temperature changing to the cubic structure was about 165℃. The polarization change according to the compressive stress and electric field was measured. Relative dielectric constant was calculated from the slope of the polarization curve applied to the electric field, and the calculated relative dielectric constant increased as the applied stress increased, and the relative dielectric constant decreased as the applied electric field increased. The strain according to the compressive stress and electric field change was measured, the piezoelectric constant was calculated from the slope of the curve, and the phase transition according to the application of pressure was confirmed. In the case of practical application as an underwater or medical ultrasonic actuator, it is necessary to properly design the magnitude of the compressive stress applied to the device and the DC bias in order to maintain linear driving.

Response Analysis of Nearby Structures with the Consideration of Tunnel Construction and Ground Conditions (터널시공 및 지반조건을 반영한 인접구조물의 거동분석)

  • Son, Moorak;Yun, Jongcheol
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.6C
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    • pp.255-263
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    • 2010
  • This paper investigates the effects of tunnelling-induced ground movements on nearby structures, considering soil-structure interactions of different construction (ground loss) and soil characteristics. The response of four-story block structures, which are subjected to tunnelling-induced ground movements, has been investigated in different construction (ground loss) and soil conditions using numerical analysis. The structures for numerical analysis has been modelled using Discrete Element Method (DEM) to have real cracks when the shear and tensile stress exceed the maximum shear and tensile strength. The response of four-story block structures has been investigated with a ground movement magnitude and compared in terms of construction (ground loss) and soil conditions considering the magnitude of deformations and cracks in structures. In addition, the damage levels, which are possibly induced in structures, has been provided in terms of construction (ground loss) and soil conditions using the state of strain damage estimation criterion (Son and Cording, 2005). The results of this study will provide a background for better understandings for controlling and minimizing building damage on nearby structures due to tunnelling-induced ground movements.

Evaluation of Characteristics of Re-liquefaction Resistance in Saturated Sand Deposits Using 1-g Shaking Table Test (1-g 진동대시험을 이용한 포화된 모래지반의 재액상화 강도 특성 평가)

  • Ha Ik-Soo;Kim Myoung-Mo
    • Journal of the Korean Geotechnical Society
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    • v.21 no.4
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    • pp.65-70
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    • 2005
  • Many case histories of re-liquefaction phenomena seem to support the idea that sand deposits, if they once have been liquefied, could be reliquefied again by a subsequent earthquake even though the earthquake is smaller than the previous one. The magnitude of the strains induced in the initial liquefaction has a significant influence on the resistance of the sample to re-liquefaction. The deposits undergoing liquefaction experience large shear strain during liquefaction. And this previous strain changes the microstructure into highly anisotropic structure such as columnlike structure and connected voids. This type of anisotropy is so unstable that it can reduce re-liquefaction resistance. It is blown that the extent of anisotropic structural change depends on the gradation characteristics of ground. The purpose of this study is to estimate the correlation between the gradation characteristics of the sand and the ratio of re-liquefaction resistance to liquefaction resistance. In this study, 1-g shaking table tests were carried out on five different kinds of sands. During the tests the values of excess pore pressure at various depths and surface settlements were measured. Re-liquefaction resistances were not affected by the initial void ratio and the effective confining pressures, and the deposits of all test sands which had once been liquefied were reliquefied in the cyclic loading number below 1 to 1.5. The ratio of re-liquefaction resistance to liquefaction resistance linearly decreased as $D_{10}/C_u$ increased, and was constant as about 0.2 above the value of $D_{10}/C_u$, 0.15 mm.

Evaluation on the Applicability of Monitoring for Urban Railway Structure Using Brillouin Optical Correlation Domain Analysis Based Distributed Optical Fiber Sensor (브릴루앙 광 상관영역 기반 분포형 광섬유를 활용한 도시철도 구조물의 모니터링 적용성 평가)

  • Chae, Deokho;Lee, Sungjin;Lee, Jin-Wook
    • Journal of the Korean GEO-environmental Society
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    • v.19 no.9
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    • pp.13-19
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    • 2018
  • Recently, there have been various problems aroused on the domestic infrastructures as the domestic cities become old. Accordingly, the national concerns grow on the urban railway and the related structures, which brings the national interests are brought on the research on the maintenance and rehabilitation of the old infrastructures. The underground structure of urban railway are checked with the strain gages or fiber brag grating (FBG) sensors on the railway. However, these methods are known to have resolution limitations on the investigations of the specified abnormal section. Therefore, the applicability of the Brillouin Optical Correlation Domain Analysis (BOCDA) based distributed fiber optic sensor system on the railway was evaluated in this study. The constructed BOCDA fiber optic sensor system shows high resolution of 10, 20, 50, 100 cm and capability of continuous monitoring on overall or specified section within 2 km range. The applicability evaluation was performed on the 250 m distribution of fiber optic sensors abandoned railway for continuous monitoring. The applicability of the system on the specified area was evaluated with wheel load testing. As a result, data loss tends to increase with the reduction of spatial resolution from 1.0 m to 0.1 m. Even though the measuring speed is reduced with lower spatial resolution, data accuracy increases on the location and deformation. The system can be applicable to various structures if the proper distribution method is invented later.

Studies on the Mutagenicity, Lipids Peroxidation and Meat Structure of Cooked Pork in Relation to Storage and Reheating using a Microwave Oven (가열조리한 돼지고기의 저장${\cdot}$Microwave 재가열에 의한 변이원성과 지질과산화 및 육조직에 관한 연구)

  • Chung Kyung-Sook;Koo Sung-Ja
    • Korean journal of food and cookery science
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    • v.20 no.6 s.84
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    • pp.643-649
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    • 2004
  • Pork was cooked using three kinds of instrument [electric pill (EG) for 5min., microwave oven (MW) for 6min. and reheated using a MW] and then extracted with $80\%$ methanol. The Ames test was performed on the methanol extracts, employing the S. typhimurium tester strain, TA100. The methanol extract of cooked pork showed high mutagenicity ion the 5.0 mg/plate without the S9 mix, but a higher mutagenicity was induced with the S9 mix With increasing refrigeration $(4^{\circ}C)$ and freezing $(-18^{\circ}C)$ periods the extracts showed higher mutagenicities and TBA values, and the same results where shown with reheating. Correlations of the mutagenicity (-S9 mix) and rancidity of the pork cooked by EG, according to storage at and $-18^{\circ}C$ and reheated by MW (1 min), were r=0.85, 0.86, 0.98 and 0.83, respectively. When the MW was used for reheating, the refrigeration storage (r=0.98) showed a higher correlation coefficient than for that stored frozen (r=0.83). From the structure of cooked pork, as observed by SEM, many vapor pathways were viewed in the pork reheated using themicrowave oven.

Nonlinear Explosion Analyses for Damage Assessments of Reinforced Concrete Structures (비선형 폭발해석에 의한 콘크리트 구조물의 손상도 평가)

  • Huh, Taik Nyung;Kim, Seong Yun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.37 no.1
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    • pp.1-7
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    • 2017
  • In general, the large loads which are applied from explosion, impact, earthquake and wind at a short time caused the materials of structures to large deformations, rotations and strains locally. If such phenomena will be analyzed, hydrocodes which can be considered fluid-structure interaction under computational continuum mechanics are inevitably needed. Also, the explosion mechanism is so complicated, it is reasonable that the behaviors of structure are predicted through explosion analyses and experiment at the same time. But, unfortunately, it is true that explosion experiments are limited to huge cost, large experiment facilities and safety problems. Therefore, in this study, it is shown that the results of explosion analyses using the AUTODYN are agreed with those of existing explosion experiments for reinforced concrete slabs within reasonable error limits. And the explosion damage of the same reinforced concrete slab are assessed for quite different reinforcement arrangement spacings, concrete cover depths, and vertical reinforcements. From the explosion analyses, it is known that the more the ratio of slab thickness to reinforcement arrangement spacing is increased, and small-diameter reinforcements are used than large-diameter reinforcements on the same reinforcement ratio, and vertical reinforcements are used, the more the anti-knock capacities are improved.

Optical Fiber-Based Hybrid Nerve Measurement System for Static and Dynamic Behavior of Structures (구조물의 정적 및 동적 거동 모니터링을 위한 광섬유 기반 하이브리드 신경망 계측 시스템)

  • Park, Young-Soo;Song, Kwang-Yong;Jin, Seung-Seop;Park, Young-Hwan;Kim, Sung-Tae
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.24 no.2
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    • pp.33-40
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    • 2020
  • Various studies have been conducted on the structural health monitoring using optical fiber. Optical fibers can be used to measure multiple and distributed strain. Among the optical fiber sensors, FBG sensor has advantages of dynamic response measurement and high precision, but the number of measurement points is limited. Distributed fiber sensors, represented by distributed Brillouin sensors, usually have more than 1000 measurement points, but the low sampling rate makes dynamic measurements impossible. In this study, a hybrid nerve sensor system using only the advantages of the FBG sensor and the distributed Brillouin sensor has been proposed. Laboratory experiments were performed to verify the proposed system, and the accuracy and reproducibility were verified by comparing with commercial sensors. Applying the proposed system, dynamic response ambient measurements are used to evaluate the global state of the structure. When an abnormal condition is detected, the local condition of the structure is evaluated by static response measurement using the distributed measurement system. The proposed system can be used for efficient structural health monitoring.