• Applied Biological Chemistry
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• v.41 no.1
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• pp.18-22
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• 1998

A mutant strain which hyperproduced thermostable ${\alpha}-amylase$ was obtained by chemical mutagenesis of Bacillus licheniformis. The mutant strain, SK-5, produced the enzyme about 50 times higher than the original strain. The mutant was longer and slimmer in shape, slower in growth compared to the original strain. Nucleotide sequence analysis of the SK-5 ${\alpha}-amylase$ gene revealed no changes in the the structural gene. The changes found in the promoter region might be responsible for the hyperproduction of the enzyme by the mutant. No structural changes in the enzyme structure could be observed when the secreted enzymes at various culture times were analyzed by Western blot.

• Korean Journal of Microbiology
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• v.39 no.3
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• pp.155-160
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• 2003

The wild type and two morphological variants of Streptomyces viridochromogenes were studied for their lipidand fatty acid compositions at different incubation temperatures. It showed that a decrease in triacylglycerol content was closely linked to the aerial mycelium formation. Phospholipids showed no characteristic changes, except that the contents of phosphatidylethanolamine were clearly high for aerial mycelium deficient strain BR2 grown at $20^{\circ}C$. The strain BR2 also presented unidentified aminolipids with various $R_{f}$ values. Among the aminolipids, ornithinolipid increased gradually during the cultivation for all strains. The changes in fatty acid compositions showed a temperature dependency that the proportion of unsaturated acids decreased as the growth temperature increased. The proportion of straight chain saturated fatty acids decreased as the aerial mycelium developed, and it was most evident for the mutant strain M13 with more extensive aerial mycelium. The mutant strain BR2 presented significantly higher level of iso branched odd numbered saturated fatty acids.

• Advanced Composite Materials
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• v.17 no.2
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• pp.125-137
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• 2008

FBG (Fiber Bragg Grating) sensors and optical fibers were embedded into CFRP dry preforms before resin impregnation in VaRTM (Vacuum-assisted Resin Transfer Molding). The embedding location was the interface between the skin and the stringer in a CFRP-stiffened panel. The reflection spectra of the FBG sensors monitored the strain and temperature changes during all the molding processes. The internal residual strains of the CFRP panel could be evaluated during both the curing time and the post-curing time. The temperature changes indicated the differences between the dry preform and the outside of the vacuum bagging. After the molding, four-point bending was applied to the panel for the verification of its structural integrity and the sensor capabilities. The optical fibers were then used for the newly-developed PPP-BOTDA (Pulse-PrePump Brillouin Optical Time Domain Analysis) system. The long-range distributed strain and temperature can be measured by this system, whose spatial resolution is 100 mm. The strain changes from the FBGs and the PPP-BOTDA agreed well with those from the conventional strain gages and FE analysis in the CFRP panel. Therefore, the fiber-optic sensors and its system were very effective for the evaluation of the VaRTM composite structures.

• 한국전기화학회:학술대회논문집
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• 2003.10a
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• pp.29-29
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• 2003

• Journal of KIBIM
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• v.9 no.3
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• pp.1-7
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• 2019

Digital twin technology has been actively developed to monitor and assess the current state of actual structures. The digital twin changes the traditional observation method performed in the field to the real-time observation and detection system using virtual online model. Thus, this study designed a digital twin model for a beam and examined the feasibility of the digital twin for bridges. To reflect the current state of the bridge, model updating was performed according to the field test data to construct an analysis model. Based on the constructed bridge analysis model, the relationship between strain and displacement was used to represent a virtual model that behaves in the same way as the actual structure. The strain and displacement relationship was expressed as a matrix derived using an approximate analytical theory. Then, displacements can be obtained using the measured data obtained from strain sensors installed on the bridge. The coordinates of the obtained displacements are used to construct a virtual digital model for the bridge. For verification, a beam was fabricated and tested to evaluate the digital twin model constructed in this study. The displacements obtained from the strain and displacement relationship agrees well with the actual displacements of the beam. In addition, the displacements obtained from the virtual model was visualized at the locations of the strain sensor.

• Journal of radiological science and technology
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• v.47 no.4
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• pp.279-286
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• 2024

Echocardiography is a widely used test to evaluate the contractility of the heart. However, this exam is cannot be said to be objective because the test relies on the examiner's visual judgment. Therefore, although a lot of efforts are being made to quantify the strain through speckle tracking echocardiography (STE), there are some limitations, and studies of conditions to supplement are needed. Through this study, we determine whether the general characteristics of the subjects affect vessel-specific strain and whether changes in various imaging conditions (general, resolution, compression, gain, chroma) affect vessel-specific strain. According to the results of the study, the general conditions obtained during the examination were generally less significant compared to other conversion conditions, and Resolution, Compress, Gain, Chroma, and LAD showed significant correlations under the Compress conditions (p < .05). Therefore, Accurate strain assessment will be made if echocardiography is analyzed using significant conditions for each stenotic vessel

• Transactions of the Korean Society of Mechanical Engineers A
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• v.22 no.1
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• pp.238-246
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• 1998

Surface residual stresses as well as wall thickness and ovality changes after U-bending process on UNS N06690 row-1 heat exchanger tubes, were estimated. Surface residual stresses were measured by Hole Drilling Method(HDM), calculating the stresses from relieved strains of 3 rosette strain gages. After bending of the tubes, dimensional tolerances for wall thickness and ovality were satisfied with ASTM requirements. Residual stresses at the extrados were introduced with compressive stress(-) by bending operations, and its maximum value reached-319 MPa in axial direction at ${\phi}=0^{\circ}$ in position. Tensile residual stresses(+) of ${\sigma}_zz=45$ MPa,${\sigma}_zz=25$ MPa were introduced in the intrados surface at position of ${\phi}=0^{\circ}$ Maximum tensile residual stress of 170 MPa was detected on the flank side at position of ,${\phi}=95^{\circ}$i.e., at apex region. It appeared that higher stress gradients were generated at the irregular transition regions. In the trend of residual stress changes with U-bend position, the extrados is related with the changes of ovality and the intrados is related with the changes of wall thickness.

• Parasites, Hosts and Diseases
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• v.21 no.2
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• pp.234-240
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• 1983

The pathogenicity of free-living amoeba, Waegleria fcwleri, is influenced according to the strain, cultural condition and host (Culbertson et at., 1968; Carter, 1970; Wong et at., 1975), Phillips (1973) demonstrated that Entamoeba histolytica became avirulent after more than 2 year maintenance in axonic culture in vitro. This study was carried out to compare the difference in pathogenicity between two strains of N. fowleri, one of a prolonged maintenance in arsenic medium and the other one obtained by serial brain passage in mice. The 0 strain was that N. fowleri had cultivated axenically more than 7 years in CGVS medium. The 2-1 strain was obtained from the brain of mouse inoculated intranasally with a strain, which was from the mouse brain infected with 0 strain, and cultured for 15 weeks until the beginning of this experiment. White male mice weighing 18-22 g were used. Mice were anesthetized by an intraperitoneal injection of about 1 mg secobarbital, and inoculated intranasally with $10{\times}$10^4 live N. fowleri trophoBoites in a $5{\;}{\mu}l$ cell suspension. Sluggish behaviour, nervousness, rotation and leg paralysis were developed earlier and more frequently in the 2-1 experimental group than the control 0 group. Pathological changes such as inflammatory and necrotic lesion were observed in the olfactory and anterior portion of brain, and these changes were more extensive in the 2-1 group. The edematous and inflammatory changes in lung were demonstrated in mice died after 13th day post-inoculation. The experimental mice of 2-1 group began to die suddenly from 7th day post-inoculation, and the survival time in 2-1 group mice was shorter than 0 group mice. The typical primary amoebic meningoencephalitis was developed in the mice inoculated intranasally with N. fowleri. The prolonged maintenance of N. fowleri amoebae in axonic CGVS medium was observed to have lost their original pathogenicity for mice, but their pathogenicity was restored by serial brain passage in mice.

• Journal of Microbiology
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• v.41 no.2
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• pp.129-136
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• 2003

The Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMPI) exhibits considerable sequence heterogeneity among EBV isolates. Seven distinct EBV strains have been defined based on sequence polymorphisms in the LMPI gene, which are designated China 1, China 2, China 3, Alaskan, Mediterranean, NC, and the B95-8 strains. In this study, we analyzed a 30-bp deletion and sequence variations in the carboxy-terminal region of the LMPl gene in 12 EBV isolates from spontaneous lym-phoblastoid cell lines derived from individuals with non-EBV associated cancers in Korea. Eleven of the 12 isolates showed a 30-bp deletion spanning LMPI amino acids 342 to 353, suggesting a high prevalence of the LMPI 30-bp deletion variant among EBV isolates in Korea. In addition, all 12 isolates had a 15-bp common deletion in the 33-bp repeat region and multiple base-pair changes relative to the prototype B95-8 EBV strain along with variations in the number of the 33-bp repeats. The bp changes at positions 168746, 168694, 168687, 168395, 168357, 168355, 168631, 168320, 168308, 168295, and 168225 were highly conserved among the isolates. Comparative analysis of sequence change patterns in the LMPI carboxy-terminal coding region identified nine 30-bp deletion variants as China 1, two deletion variants as a possible interstrain between the Alaskan and China 1 strains, and a single undeleted variant as a possible variant of the Alaskan strain. These results suggest the predominance of the China 1 EBV strain in the Korean population.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.108-113
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• 2004

Korea Gas Corp. has developed the design technology of the LNG storage tank. The membrane to be applied inside of the LNG storage tank is provided with corrugations to absorb thermal contraction and expansion caused by LNG temperature changes. It is very important to measure their thermal strains under LNG temperatures by analytical and experimental stress analysis of the membrane. We have developed a stress measurement system using strain gages and measured the strain during cooldown and storing the LNG. We also analyzed the measured data by comparison with the FEM data. On the basis of these results, we could design and assure the application of the Kogas Membrane to large scale LNG storage.