• Bulletin of the Korean Chemical Society
• /
• 제35권3호
• /
• pp.933-937
• /
• 2014

A derivative of Mycobacterium bovis Bacillus Calmette-Guerin (BCG) has been used for the preparation of tuberculosis vaccines. To establish a Korean tuberculosis vaccine derived from BCG-Pasteur $1173P_2$, genome sequencing of a BCG-Korea strain was completed by Joung and coworkers. A comparison analysis of the genome sequences of the BCG-Pasteur $1173P_2$ and BCG-Korea strains showed marginal increases in the total genome length (~0.05%) and the number of genes (~4%) in the BCG-Korea genome. However, how the genomic changes affect the BCG-Korea protein expression levels remains unknown. Here, we provide evidence of the proteomic alterations in the BCG-Korea strain by using a SWATH-based mass spectrometric approach (Sequential Window Acquisition of all THeoretical mass spectra). Twenty BCG proteins were selected by top-rank identification in the BCG proteome analysis and the proteins were quantified by the SWATH method. Thirteen of 20 proteins showing significant changes were enough to discriminate between the two BCG proteomes. The SWATH method is very straightforward and provides a promising approach owing to its strong reliability and reproducibility during the proteomic analysis.

• 한국암반공학회:학술대회논문집
• /
• 한국암반공학회 2000년도 암반공학문제의 수치해석(Numerical Analysis in Rock Engineering Problems)
• /
• pp.139-146
• /
• 2000

지하 채굴에 따른 침하와 수리전도도의 변화 양상에 관하여 조사를 하였다. 채굴 후 암반내 발생하는 변형률과 변형률 의존 수리전도도와의 상호 관계를 나타내는 식을 얻기 위하여 절리 암반을 등가 다공질 매질로 고려하였으며, 단일 절리의 수리전도도는 평행판 이론을 적용하여 결정하였다. 해석 결과에 따르면 채굴 후 암반내 수리전도도의 변화는 침하에 의해 발생한 변형률에 직접적으로 연관이 있는 것으로 나타났다. 채굴적의 폭을 증가시킴에 따라 최대 침하량과 수리전도도의 크기가 증가하였다. 절리 간격은 수리전도도의 변화에 영향을 주는 것으로 나타났다.

• 터널과지하공간
• /
• 제10권3호
• /
• pp.387-394
• /
• 2000

지하 채굴에 따른 침하와 수리전도도의 변화 양상에 관하여 조사를 하였다. 채굴 후 암반내 발생하는 변형률과 변형률 의존 수리전도도와의 상호 관계를 나타내는 식을 얻기 위하여 절리 암반을 등가 다공질 매질로 고려하였으며, 단일 절리의 수리전도도는 평행판 이론을 적용하여 결정하였다. 해석 결과에 따르면 채굴 후 암반내 수리전도도의 변화는 침하에 의해 발생한 변형률에 직접적으로 연관이 있는 것으로 나타났다. 채굴적의 폭을 증가시킴에 따라 최대 침하량과 수리전도도의 크기가 증가하였다. 절리 간격은 수리전도도의 변화에 영향을 주는 것으로 나타났다.

• Smart Structures and Systems
• /
• 제3권4호
• /
• pp.475-494
• /
• 2007

This paper deals with the development of an innovative distributed construction system based on smart prefabricated concrete elements for the real-time condition assessment of civil infrastructure. So far, two reduced-scale prototypes have been produced, each consisting of a $0.2{\times}0.3{\times}5.6$ m RC beam specifically designed for permanent instrumentation with 8 long-gauge Fiber Optic Sensors (FOS) at the lower edge. The sensing system is Fiber Bragg Grating (FBG)-based and can measure finite displacements both static and dynamic with a sample frequency of 625 Hz per channel. The performance of the system underwent validation in the laboratory. The scope of the experiment was to correlate changes in the dynamic response of the beams with different damage scenarios, using a direct modal strain approach. Each specimen was dynamically characterized in the undamaged state and in various damage conditions, simulating different cracking levels and recurrent deterioration scenarios, including cover spalling and corrosion of the reinforcement. The location and the extent of damage are evaluated by calculating damage indices which take account of changes in frequency and in strain-mode-shapes. The outcomes of the experiment demonstrate how the damage distribution detected by the system is fully compatible with the damage extent appraised by inspection.

• Fisheries and Aquatic Sciences
• /
• 제15권3호
• /
• pp.233-239
• /
• 2012

Hematological analysis can provide crucial information for monitoring the health of fish. However, there is no current information available regarding hematological changes in olive flounder following infection by Vibrio scophthalmi. In this study, hematological and biochemical alterations were determined in olive flounder infected by the high virulence strain (HVS) and low virulence strain (LVS) of V. scophthalmi. Survival in serum, skin mucus, and macrophages of olive flounder was also compared between the HVS and LVS. The results demonstrated that the hematocrit value in infected fish declined from 23.4% at 0 h to 18.0% at 168 h post infection. The total protein concentration in fish infected with the HVS was significantly higher than in fish infected with the LVS and a non-infected control. Lysozyme activity was significantly different between infected and control fish. The HVS survived in serum and cell numbers increased substantially, while cell numbers of the LVS in serum decreased. These changes in hematological characteristics in fish infected by V. scophthalmi can be used as an effective and sensitive index to monitor the physiological and pathological conditions of fish. The survival and reproduction of V. scophthalmi in host serum, skin mucus, and macrophages play a major role in systemic infection and can serve as a virulence indicator for different strains.

• Structural Engineering and Mechanics
• /
• 제39권2호
• /
• pp.187-206
• /
• 2011

This paper uses dynamic computer simulation techniques to develop and apply a multi-criteria procedure using non-destructive vibration-based parameters for damage assessment in truss bridges. In addition to changes in natural frequencies, this procedure incorporates two parameters, namely the modal flexibility and the modal strain energy. Using the numerically simulated modal data obtained through finite element analysis of the healthy and damaged bridge models, algorithms based on modal flexibility and modal strain energy changes before and after damage are obtained and used as the indices for the assessment of structural health state. The application of the two proposed parameters to truss-type structures is limited in the literature. The proposed multi-criteria based damage assessment procedure is therefore developed and applied to truss bridges. The application of the approach is demonstrated through numerical simulation studies of a single-span simply supported truss bridge with eight damage scenarios corresponding to different types of deck and truss damage. Results show that the proposed multi-criteria method is effective in damage assessment in this type of bridge superstructure.

• 전기학회논문지
• /
• 제60권3호
• /
• pp.594-599
• /
• 2011

A SAW strain sensor based on Shear Horizontal wave with an 92 MHz central frequency was developed. It consists of SAW sensor, PCB substrate and bonding material (Loctite 401). External force applied to PCB substrate bonded to a piezoelectric substrate induces strain at the substrate surface, which causes changes in the elastic constant and density of the substrate and hence the propagation velocity of the SAW. The change in the velocity of the SAW result in a frequency shift of the sensor and by measuring a frequency shift, we can extract the strain induced by the external force. The $41^{\circ}$ YX $LiNbO_3$ was used because it has a Leaky shear horizontal(SH) wave propagation mode and a high electromechanical coupling coefficient ($K^2$=17.2%). And to compare with Rayleigh wave mode, $128^{\circ}$ YX $LiNbO_3$ was used. And to make a stable and low insert loss, Split IDT structure was used. The obtained sensitivity and linearity of the SAW strain sensor in the case of Split IDT were measured to be 17.2 kHz / % and 0.99, respectively.

• 소성∙가공
• /
• 제17권6호
• /
• pp.420-428
• /
• 2008

The effect of loading path changes on the strain and mechanical properties of a commercial pure aluminum was studied using flat rolling and groove rolling. Material during flat rolling undergoes a continuous monotonic compressive loading, while one during groove rolling experiences a series of cross compressive loading. Four-pass flat rolling and groove rolling experiment are designed such that the aluminum undergoes the same amount of the strain at each pass. The rolling experiment was performed at room temperatures. Specimens for tensile test are fabricated from the plate and bar rolled. In addition, the strain distribution for the plate and bar cold rolled specimens is also calculated by finite element method. The results reveal that differences of loading path attributed by monotonic loading(flat rolling) and cross loading(groove rolling) significantly influence the mechanical properties such as yield stress, ultimate tensile stress, strain hardening and elongation. It is clear that the different loading path can give raise to change the deformation history, although it is deformed with same amount of strain for same material.

• Journal of Welding and Joining
• /
• 제31권6호
• /
• pp.37-43
• /
• 2013

This study targets to make clear the connection between MS (Martensite start) point and welding shrinkage. We approved that a Martensite-transformed weldment may not yield state under low MS point, but also admitted the limitation of numerical calculation by inherent strain approach or thermal strain approach. Therefore, new thermal strain formulae during cooling stages were made. As a thermal strain is obtained by integrating thermal extension coefficient, a constant of integration should be decided. In our suggested formulae, the origin was based on totally remained austenite, and added strain from volume changes in Martensite transformation was based on totally transformed ferrite. Through the suggested methodology, It is verified that an MS point under a critical temperature can let weld shrinkage relax and the critical value can be obtained. For supporting this process, 15 weld-consumables were made, were tested by fillet type and were measured. As a result, a positive correlation between MS point and level of weld-distortion was obtained, but it was rather weak.

• 한국정밀공학회지
• /
• 제12권3호
• /
• pp.101-109
• /
• 1995

Axisymmetric and quasi-static finite element analysis of an inflated tire rotating with constant angular velocity and contact to road has been performed. Centrifugal force effect was added to load stiffness matrix and equation of effective material properties were calculated by the Halpin-Tsai formulation. In this report, radial truck/bus tire was analyzed. It was inflated and rotated at speeds up to 140 km/h. Then, contact problem was performed to calculate stress-strain field of tire wiht flat rigid road under the load due to the self-weight of a vehicle. Significant changes of stress-strain field of tire were observed in the finite element analysis. Shear stress, strain and strain energy density were rapidly increased at the dege of #2 belt at freely rotating state. This concentrated stress and strain made belt edge sparation. Under the condition of flat riged road contact, strain energy density of #2 belt, carcass turn-up part were concentrated and bigger values than only freely rotation state. Therefore, dynamic behaivor of tire has to considered as design factors which are affected to belt edge separation and bead breakage.