• Title/Summary/Keyword: Strain analysis

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Isolation and Characterization of Extracellular Polymeric Substances (EPS)-producing bacteria for restoration of burnt forest soils (산불토양복원을 위한 Extracellular Polymeric Substances (EPS) 생성세균의 분리, 동정 및 특성에 관한 연구)

  • Lee, Gun-Young;Song, In-Geun;Chung, Jae-Chun;Kim, Young-Jun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.12 no.4
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    • pp.139-147
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    • 2004
  • We have isolated two bacterial strains, FM-02 and AL-02, which produced EPS from forest soil for the restoration of forest fire by promoting soil aggregation. FM-02 was found to be Gram negative rod and belong to Beta Proteobacterium sp. through 16s-rDNA sequence analysis, and AL-02 was Gram positive rod and showed 81% of similarity to Zoogloea sp. through the analysis of 16s-rDNA sequence. FM-02 and AL-02 produced about 1.8g and 8.3g of EPS, respectively, per 1L of culture as dry weight. Flocculation activity (FA) was also measured in two strains. FM-02 showed 2.31 FA against active carbon, and AL-02 showed 6.21 FA against kaolin clay. From these results, we expect that AL-02 strain will be applied as a good biological material for the reduction of forest soil erosion by wild and rain after fire through promoting coagulation of soil particles.

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Antibiotics Resistance and Molecular Analysis of Enterococcus Isolated from the Han-river in Korea (한강에서 분리한 장구균의 항생제 내성과 분자생물학적 분석)

  • Kwon, Oh-Min;Kim, Mal-Nam
    • Korean Journal of Microbiology
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    • v.48 no.2
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    • pp.116-124
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    • 2012
  • Identification was performed in March 2008 for the 76 Enterococcus strains isolated from the Han-river, which is used as water supply for Seoul citizens. The antibiotic susceptibility, antibiotic resistant structural analysis, trans-conjugation, pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were also carried out for the isolated strains. Among the isolated strains, 25 strains were E. casseliflavus, 4 strains were E. faecalis and 1 strain was E. hirae. Investigation of antibiotic susceptibility indicated that 15 strains demonstrated tolerance against vancomycin, and that 11 strains of E. faecium and 4 strains of E. casseliflavus were VRE. The vanA gene detection of the VRE strains revealed that 6 E. faecium strains were vancomycin-resistant Enterococcus faecium (VREF) possessing vanA. Analyses of transposon Tn1546 structure containing vanA demonstrated that Km36 and Km37 belonged to Tn1 type, Km20 and Km38 was Tn2 type, and Km39 and Km40 was Tn3 type. PFGE disclosed that among the 6 VREF strains, Km36 and Km37 exhibited equivalent subtype, while the rest 4 strains showed subtypes different to each other. MLST for the 6 VREF strains disclosed that 3 strains were ST78, while the rest 3 strains were ST18, ST192 and ST230, respectively. All these clonal complexes were derived from CC17 which has been isolated from clinical sources. 4 strains belonged to CC78, while the rest 2 strains were CC18 and CC192, respectively.

A Study on the Determination of Reference Parameter for Aircraft Impact Induced Risk Assessment of Nuclear Power Plant (원전의 항공기 충돌 리스크 평가를 위한 대표매개변수 선정 연구)

  • Shin, Sang Shup;Hahm, Daegi;Choi, In-Kil
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.27 no.5
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    • pp.437-450
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    • 2014
  • In this study, we developed a methodology to determine the reference parameter for an aircraft impact induced risk assessment of nuclear power plant (NPP) using finite element impact analysis of containment building. The target structure used to develop the method of reference parameter selection is one of the typical Korean PWR type containment buildings. We composed a three-dimensional finite element model of the containment building. The concrete damaged plasticity model was used for the concrete material model. The steels in the tendon, rebar, and liner were modeled using the piecewise-linear stress-strain curves. To evaluate the correlations between structural response and each candidate parameter, we developed Riera's aircraft impact force-time history function with respect to the variation of the loading parameters, i.e., impact velocity and mass of the remaining fuel. For each force-time history, the type of aircraft is assumed to be a Boeing 767 model. The variation ranges of the impact velocity and remaining fuel percentage are 50 to 200m/s, and 30 to 90%, respectively. Four parameters, i.e., kinetic energy, total impulse, maximum impulse, and maximum force are proposed for candidates of the reference parameter. The wellness of the correlation between the reference parameter and structural responses was formulated using the coefficient of determination ($R^2$). From the results, we found that the maximum force showed the highest $R^2$ value in most responses in the materials. The simplicity and intuitiveness of the maximum force parameter are also remarkable compared to the other candidate parameters. Therefore, it can be concluded that the maximum force is the most proper candidate for the reference parameter to assess the aircraft impact induced risk of NPPs.

Evaluation of Split Tension Fatigue Test Method for Application in Concrete (콘크리트의 쪼갬인장 피로실험방법 제안 및 적용성 평가)

  • Kim Dong-Ho;Lee Joo-Hyung;Jeong Won-Kyong;Yun Kyong-Ku
    • Journal of the Korea Concrete Institute
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    • v.16 no.1 s.79
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    • pp.27-35
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    • 2004
  • Most of concrete fatigue tests currently used are flexural tension or compression methods to investigate the tensile or compressive properties, respectively. However, the concrete pavement or concrete slab is actually subjected to a combined stress condition such as biaxial or triaxial. The split tension test may result in similar stress condition to biaxial stress condition. The purposes of this study were to evaluate the split tension fatigue test method for application in concrete. These were done by a finite element analysis and experimental series. The results were as follows: The optimum configuration of split tension fatigue test was a cylinder of 15cm in diameter and 7.5cm in thickness, which had a little different thickness compared to the KS standard cylinder of ${\phi}15{\times}30cm$. The concrete stress ratio of compressive against horizontal from FEA was 3.1, while that from theory was 3.0. The stress distributions of mortar and steel were almost similar at different thicknesses. The measured static split tensile strengths of concrete and mortar were quite similar at 30cm and 7.5cm thickness cylinders. The measured stress-strain relationship showed their consistency at all specimens regardless of thickness, and confirmed the results from FEA. As a results, the concrete split tension specimen, cylinder of 15cm in diameter and 7.5cm in thickness, could be used at fatigue test because of its accuracy, simplicity and convenience.

Analysis of Seismic Response of the Buried Pipeline with Pipe End Conditions (II) (단부 경계조건을 고려한 매설관의 동적응답 해석 (II))

  • Lee, Byong-Gil;Park, Byung-Ho;Jeong, Jin-Ho
    • Proceedings of the Korean Geotechical Society Conference
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    • 2005.03a
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    • pp.328-337
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    • 2005
  • This work reports results of our study on the dynamic responses of the buried pipelines both along the axial and the transverse directions under various boundary end conditions. We have considered three cases, i.e., the free ends, the fixed ends, and the fixed-free ends for the axial direction, and three more cases including the guided ends, the simply supported ends, and the supported-guided ends for the transverse direction. In order to investigate the effect of the boundary end conditions for the dynamic responses of the buried pipeline, we have devised a computer program to find the solutions of the formulae on the dynamic responses (displacements, axial strains, and bending strains) under the various boundary end conditions considered in this study. The dynamic behavior of the buried pipelines for the forced vibration is found to exhibit two different forms, a transient response and a steady state response, depending on the time before and after the transfer of a seismic wave on the end of the buried pipeline. The former is identified by a slight change in its behavior before the sinusoidal-shaped seismic wave travels along the whole length of the pipeline whereas the latter by the complete form of a sinusoidal wave when the wave travels throughout the pipeline. The transient response becomes insignificant as the wave speed increases. We have observed a resonance when the mode wavelength matches the wavelength of the seismic wave, where the mode number(k) of resonance for the axial direction is found to be $\overline{\omega}/{\pi}V+1/2$ for the fixed-free ends, $\overline{\omega}/{\pi}V+1$ for the free ends, and $\overline{\omega}/{\pi}V$ for the fixed ends, respectively. By adding 10 more modes to the mode number(k) of resonance, we were able to study all the dynamic responses of the buried pipeline for the axial direction. On the other hand, we have not been able to observe a resonance in the analysis for the transverse direction, because the dynamic responses are found to vanish after the seventh mode. From the results of the dynamic responses at the many points of the pipeline, we have found that the responses appeared to be dependent critically on the boundary end conditions. Such effects are found to be most prominent especially for the maximum values of the displacement and the strain and its position.

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Molecular Genetic Characteristics of Methicillin-Resistant Staphylococcus aureus Isolated from University Campus Environment and Students

  • Park, Heechul;Park, Sung-Bae;Kim, Junseong;Jeon, Hyeonjeong;Choi, Sein;Lee, Seungyeon;Oh, Eunchong;Hwang, Soenghwi;Kim, Hyunjung;Kim, Jungho;Kim, Sunghyun
    • Biomedical Science Letters
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    • v.26 no.3
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    • pp.230-237
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    • 2020
  • Staphylococcus aureus (S. aureus) is known as a bacterium that can cause skin infections, respiratory system infections, and sinusitis; however, it can exist as a normal flora rather than a pathogen. Recently, methicillin-resistant S. aureus (MRSA) infections have emerged in the community as a new variant of community-associated (CA)-MRSA. In the present study, S. aureus and MRSA were isolated and cultured by collecting samples from facilities and environments where students and educational personnel have multiple contacts on university campuses; specifically, the nostrils and hands of college students were tested from July to September of 2019. The molecular properties of the isolated MRSA were analyzed, and the one MRSA strain was isolated from the university campuses. One MRSA that was isolated and cultured on campus was the mec complex group A and staphylococcal cassette chromosome (SCC) mec type II, which is a characteristic of healthcare-associated (HA)-MRSA, and SCCmec type V, which is a characteristic of CA-MRSA. This result was similar to other studies wherein the SCCmec type II was detected in SCCmec typing analysis in CA-MRSA. To confirm whether there is a new variant of CA-MRSA in the Republic of Korea, additional follow-up studies on the analysis of virulence factors of MRSA are needed by additionally separating CA-MRSA from the body parts of university students and educational personnel.

Analyses on the Status of Eggshell Breakage in the Step of Egg Conveyer System of the Laying Hen House (산란계사의 계란 이송단계에 따른 파란발생 실태분석)

  • Oh, K.Y.;Ruy, B.G.;Noh, J.S.;Choi, D.S.;Choe, K.J.
    • Journal of Animal Environmental Science
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    • v.14 no.1
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    • pp.47-52
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    • 2008
  • Broken eggs not only cause the economic loss in the egg producing industry, but also may not be good for the consumer's health. In order to identify the causes of eggshell breakage in laying hen houses, analyses on the status of eggshell breakage in the step of egg conveyer system of the laying hen house were conducted. The survey of eggshell breakage rate was implemented by four different stages egg conveying system. In the results, the breakage rate showed 4.7% in the egg collection belt, 2.5% in the egg elevator, 1.7% in the egg grading system and 0.9% in the egg conveyor belt. In particular, to Hy-line Brown variety, as the hens' raising week old increased, the breakage rate of eggshell increased while the strength of eggshell decreased. In the regression analysis between the breakage rate of eggshell and the strength of eggshell, the coefficient of determination ($R^2$) by the test of significance was computed as 0.7, therefore the changes of the strength of eggshell affects the breakage rate of eggshell. However, between the strength of eggshell and thickness of egg shell, and between the strength of eggshell and strain of eggshell, there was no significant difference detected in the regression analysis.

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Genetic Diversity, Pathogenicity, and Fungicide Response of Fusarium oxysporum f. sp. fragariae Isolated from Strawberry Plants in Korea (국내 딸기 시들음병균 Fusarium oxysporum f. sp. fragariae의 유전적 다양성, 병원성과 살균제 반응)

  • Nam, Myeong Hyeon;Kim, Hyun Sook;Park, Myung Soo;Min, Ji Young;Kim, Heung Tae
    • Research in Plant Disease
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    • v.26 no.2
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    • pp.79-87
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    • 2020
  • Fusarium wilt caused by Fusarium oxysporum f. sp. fragariae (Fof) is the most important diseases of a strawberry field in Korea. We surveyed phylogenetic analysis, pathogenicity test, and fungicide response about Fof isolates isolated from Korea. Twenty-seven isolates of F. oxysporum isolated from strawberry plants were conducted in this study. Specific amplification by Fof specific primer was confirmed in all 26 isolates except Fo080701 isolate. The nuclear ribosomal intergenic spacer region and the translation elongation factor EF-lα gene sequences of isolates revealed three main lineages. Most of all isolates were contained DNA lineage group 1, but 2 and 3 group was shown only one and three isolates, respectively. All isolates were shown in pathogenicity with cv. Seolhyang. The EC50 mean values of prochloraz ranged 0.02-0.1 ㎍/ml except for Fo080701 and effectively inhibited mycelial growth at low concentrations. The EC50 value of metconazole was also 0.04-0.22 ㎍/ml, showing a similar inhibitory effect to that of prochloraz. The EC50 value of pyraclostrobin was 0.23-168.01 ㎍/ml, which was different according to the strain. In the field trial, boscalid+fludioxonil, fluxapyroxad+pyraclostrobin, and prochloraz manganese were selected as the effective fungicides for controlling Fusarium wilt.

A Numerical and Experimental Study on Structural Performance of Noncomposite and Composite Eco-Arch Structures subjected to Concentrated Loads (집중하중을 받는 비합성.합성 생태아치구조물의 성능평가를 위한 수치해석 및 모형실험 연구)

  • Kim, Yong-Hee;Park, Jong-Sup;Lee, Young-Ho;Oh, Min-Su
    • Journal of Korean Society of Steel Construction
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    • v.22 no.2
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    • pp.173-183
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    • 2010
  • In this study, noncomposite and composite eco-arch structures with I-beams and precast concrete(PC) decks were investigated. Four finite-element models(a steel-girder model, a steel-girder-and-several-PC-panels model, a three-steel-girder model, and a three-steel-girder-and-several-PC-panels model) using a general finite-element program, ABAQUS, were reviewed to predict the strength of the noncomposite and composite arch structures. Based on the results of the finite- element analysis, the behaviors of the four models were investigated, and deflection and strain gauges for the experimental specimen consisting of three steel girders and several PC panels were set up to obtain the ultimate strength. The ultimate strength of the specimen was estimated to be 1,961kN. The ultimate strength was much larger than the 1,380-kN load calculated using AASHTO LRFD Bridge Design Specifications(2007). The noncomposite and composite arch bridges were found to have enough strength for safety.

Biaxial Interaction and Load Contour Method for Reinforced Concrete C- and H-shaped Structural Walls (C형 및 H형 철근콘크리트 구조벽체의 2축 상호작용과 등하중법)

  • Nam, Hye-Sung;Eom, Tae-Sung
    • Journal of the Korea Concrete Institute
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    • v.29 no.2
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    • pp.189-200
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    • 2017
  • Nonplanar structural walls with C-shaped and H-shaped sections have been used as an efficient lateral force-resisting system for building structures. Since the nonplanar walls are subjected to axial load and bending moments about two orthogonal axes, complicated section analysis is required for flexure-compression design. In the present study, a straightforward design method for biaxially loaded C- and H-shaped walls was proposed by modifying the existing load contour method for columns with symmetric solid sections. For this, a strain compatibility section analysis program that can calculate biaxial moment strengths of arbitrary wall section was developed and its validity was verified by comparing with existing test results. Then, through parametric study, the interaction of biaxial moments at constant axial loads in prototype C- and H-shaped walls was investigated. The results showed that, due to unsymmetrical geometry of the wall sections, the biaxial interaction was significantly affected by the moment directions and axial loads. From those investigations, non-dimensional contour equations of the biaxial moments at constant axial loads for C- and H-shaped walls were suggested. Further, design examples using the proposed contour equations were given for engineering practice.