• Title/Summary/Keyword: Strain analysis

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Complementary DNA Cloning and Nucleotide Sequence Analysis of Coat Protein Gene from TMV Tomato Strain (토마토에서 분리된 담배 모자이크 바이러스 외피단백질 유전자의 cDNA 클로닝 및 염기서열 분석)

  • 이청호;이영기;강신웅;박은경
    • Journal of the Korean Society of Tobacco Science
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    • v.18 no.2
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    • pp.101-106
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    • 1996
  • Tobacco mosaic virus (TMV) tomato strain was isolated from tomato "Seo-Kwang" in Korea. The virion was purified by density gradient centrifugation, and total viral RNA was isolated from the purified particles. Coat protein (CP) cDNA of the virus was synthesized by RT-PCR, and the purified cDNA fragment was subcloned to pBluescript II SK-. The analysis of nucleotide sequence showed that this cDNA was 693 nucleotides long from the insert of clone p1571 and p1572 which contain complete codons of the viral coat protein gene (474 nucleotides) and 3' untranslated region. The nucleotides of coat protein encoding cDNA of the strain were 6 nucleotides less than that of TMV common strain isolated from tobacco plant in Korea. The CP gene showed 70% maximum homology with that of the common strain in the nucleotide level and 86% maximum homology in amino acid level.cid level.

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Evaluation of Fatigue Strain Intensity Factor on Fatigue Crack Propagation Rate (da/dN) (금속 재료의 피로 균열 전파 속도(da/dN) 평가를 위한 변형율 확대 계수의 유효성 검토)

  • 유재환;최재강;손종동
    • Journal of the Korean Society of Safety
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    • v.11 no.2
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    • pp.3-8
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    • 1996
  • Fatigue fracture is the cyclic fracture phenomena at a very small local area near a crack tip. Therefore, the detailed quantitative experimental analysis about local cyclic strain distribution near a crack tip is prerequisite In order to make an effective parameter able to account for fatigue fracture problems. However, there are few reports on detailed quantitative experimental analysis of a local cyclic strain distribution near a crack tip, because of experimental difficulties. In this study, the distribution of local fatigue strains near a fatigue crack tip was in detail studied using by fine dot grid strain measurement method. From these results, a single parameter, which characterizes local fatigue strain field, was proposed. In addition, this parameter was applied to evaluate the fatigue crack propagation rate.

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Analysis of Plastic Deformation Behavior during Groove pressing (Groove Pressing 공정을 통한 소성 변형 거동 연구)

  • Yoon, S.C.;Krishnaiah, A.;Chakkingal, U.;Kim, H.S.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2008.05a
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    • pp.425-426
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    • 2008
  • Elasto-plastic finite element analysis was carried out for analyzing the severe plastic deformation behavior of copper specimens during groove pressing. Deformation localization was studied in terms of strain variations along the longitudinal direction. Plastic strain is lower at the local interface between the shear and the flat regions, which receive very little shear during the pressing cycle. Strain localization is more intensified with the number of rove pressing cycles, although the average strain level increases.

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Full field strain measurements of composite wing by digital image correlation

  • Pagani, A.;Zappino, E.;de Miguel, A.G.;Martilla, V.;Carrera, E.
    • Advances in aircraft and spacecraft science
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    • v.6 no.1
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    • pp.69-86
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    • 2019
  • This paper discusses the use of the Digital Image Correlation (DIC) technique for the displacement and strain measurements of a wet lay-up composite wing. As opposed to classical strain gages, DIC allows to conduct full field strain analysis of simple to complex structural parts. In this work, wing-up bending tests and measurements of the composite wing of the Dardo Aspect by CFM Air are carried out through an ad-hoc test rig and the Q-400 DIC system by Dantec Dynamics. Also, the results are used to validate a finite element model of the structure under investigation.

Cladophialophora lanosa sp. nov., a New Species Isolated from Soil

  • Das, Kallol;Lee, Seung-Yeol;Jung, Hee-Young
    • Mycobiology
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    • v.47 no.2
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    • pp.173-179
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    • 2019
  • Cladophialophora is characterized by simple hyphomycetes with brown hyphae that give rise to branched chains of pale brown conidia and shows affinities with the Herpotrichiellaceae. A fungal strain belonging to the genus Cladophialophora was isolated from soil in Daegu, Korea. This strain produces numerous greenish to dark black lanose aerial mycelia with hair like structures. It is morphological similar to C. chaetospira, C. inabaensis, and C. multiseptata; however, the conidiophores and conidia sizes of the newly isolated strain (KNU16-032) are clearly different from them. The novelty of the strain was also confirmed based on phylogenetic analysis using the data sets of the internal transcribed spacer region of and the partial sequence of 28S ribosomal DNA region along with the cultural characteristics. Because morphological observations and phylogenetic analysis indicated that the strain is distinct from previously known Cladophialophora species, we propose this species as a new species Cladophialophora lanosa sp. nov., and provide the detailed descriptions in this study.

Delamination analysis of inhomogeneous viscoelastic beam of rectangular section subjected to torsion

  • Victor I. Rizov
    • Coupled systems mechanics
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    • v.12 no.1
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    • pp.69-81
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    • 2023
  • This paper considers a delamination analysis of a statically undetermined inhomogeneous beam structure of rectangular section with viscoelastic behavior under torsion. The beam is built in at its two ends. The beam has two longitudinal inhomogeneous layers with a delamination crack between them. A notch is made in the upper crack arm. The external torsion moment applied on the beam is a function of time. Under these conditions, the beam has one degree of indeterminacy. In order to derive the strain energy release rate, first, the static indeterminacy is resolved. Then the strain energy release rate is obtained by analyzing the balance of the energy with considering the viscoelastic behavior. The strain energy release rate is found also by analyzing the compliance of the beam for checkup. Solution of the strain energy release rate in a beam without a notch in the upper crack arm is derived too. In this case, the beam has two degrees of static indeterminacy (the torsion moment in the upper crack arm is treated as an additional internal redundant unknown). A parametric investigation of the strain energy release rate is carried-out.

Experiment and simulation analysis on full scale double-layer concrete shell

  • Thanh Quang Khai Lam;Thi My Dung Do
    • Computers and Concrete
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    • v.31 no.1
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    • pp.9-21
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    • 2023
  • The published studies usually used analytical method, numerical methods or experimental method to determine the stress-strain state and displacement of the single-layer or multi-layer curved shell types, but with a small scale model. However, a full scale multi-layer doubly curved concrete shell roof model should be researched. This paper presents the results of the experiment and simulation analysis involving stress-strain state, sliding between layers, the formation and development of the full scale double-layer doubly curved concrete shell roof when this shell begins to crack. The results of the this study have constructed the load-sliding strain relationship; strain diagram; stress diagram in the shell layers; the Nx, Ny membrane force diagram and deflection of shell. Thisresults by experimental method on a full scale model of concrete have clarified the working of multi-layer doubly curved concrete shell roof. The experimental and simulation results are compared with each other and compared with the Sap2000 software.

Approximate residual stress and plastic strain profiles for laser-peened alloy 600 surfaces

  • Eui-Kyun Park ;Hyun-Jae Lee ;Ju-Hee Kim ;Yun-Jae Kim
    • Nuclear Engineering and Technology
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    • v.55 no.4
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    • pp.1250-1264
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    • 2023
  • This paper presents approximate in-depth residual stress and plastic strain profiles for laser-peened alloy 600 surface via FE analysis. In approximations, effects of the initial welding residual stress and the number of shots are quantified. Based on FE analysis results, residual stress profiles are quantified by two variables; the maximum difference in stress before and after LSP, and the depth up to which the compressive residual stress exists. Plastic strain profiles are quantified by one variable, the maximum equivalent plastic strain at the surface. The proposed profiles are validated by comparing with published LSP experimental results for welded plates. Effects of the initial welding residual stress and the number of shots on these variables are discussed. The proposed profile can be directly applied to predict the mitigation effect of LSP on PWSCC and to efficiently perform structural integrity assessment of laser peened nuclear components.

Sequence Analysis of the Gene Encoding gp55 Protein of Suri Strain, an Attenuated Classical Swine Fever (Hog Cholera) Virus (Classical Swine Fever (Hog Cholera) Virus 약독순화주 (Suri 주)의 gp55 Gnen 염기서열 분석)

  • Kim, Kui-Hyun;Chang, Kyung-Soo;Kang, Kyong-Im;Lee, Byung-Hyung;Park, Jong-Hyeon;An, Soo-Hwan;Jun, Moo-Hyung
    • The Journal of Korean Society of Virology
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    • v.28 no.4
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    • pp.303-316
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    • 1998
  • An attenuated classical swine fever virus (CSFV), Suri strain, is a variant derived from a vaccine virus, LOM strain. This study was performed to elucidate the molecular biologcal properties of CSFV Suri strain, and to obtain the basic data for molecular epidemiological approaches for the disease. The truncated form of gp55 gene without the C-terminal transmembrane domain, in size of 1,023bp, was amplified by RT-PCR and sequenced by dye terminator cyclic sequencing method, and inserted into BamHI site of pAcGP67B baculovirus vector, establishing a cloned pAcHEG plasmid. By the nucleotide sequences determined, 341 amino acid sequences were predicted. As compared the nucleotide and amino acid sequences of gp55 of Suri with the various CSFV, Suri strain showed the high homology over 99.1% with ALD and LOM strains, but comparably the lower homology with Alfort and Brescia. In comparison of amino acid sequence in variable domain of gp55 protein, the similar tendency of homology was observed. In hydrophobicity analysis, all of four CSFV strains revealed the analogous patterns of hydrophobicity. The numbers and locations of N-glycosylation site and cysteine residues in gp55 were analyzed, those of Suri strain being coincident with ALD and LOM strains. The results suggest that gp55 in Suri strain has the high similarity to those in ALD and LOM strains in terms of the nucleotide and amino acid sequences and the functional properties of gp55 protein.

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Numerical and Experimental Studies on Thermal Strain Analysis of Al Alloy Casting Mold using Metal Foundry (금형주조기를 이용한 알루미늄 합금 금형의 수치해석적 열변형 해석과 실험에 관한 연구)

  • Oh, Yool-Kwon;Kim, Yong-Bum;Yoon, Hee-Sung
    • Proceedings of the KSME Conference
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    • 2007.05b
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    • pp.2050-2054
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    • 2007
  • This study numerically and experimentally investigated on thermal strain analysis of aluminum alloy casting mold using metal foundry. To predict the numerical result of thermal strain in Al alloy casting mold during the cooling process, it is performed the investigation of temperature distribution, stress and displacement based on the physical properties of Al alloy. In results of this study, Al alloy casting mold represented rapidly cooling graph during initial 20minutes after beginning cooling process, therefore value of stress and displacement is rapidly changed during initial 20minutes after beginning cooling process. In addition to, temperature distribution obtained by experiment confirmed corresponding pattern then compared numerical analysis with experiment. These results are distribute to make the effective and the high precision casting mold.

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