• Title/Summary/Keyword: Strain Rings

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Morphological and Phylogenetic Characteristics of a Nematophagous Fungus, Drechslerella brochopaga Kan-23 (국내 미기록종 선충포식성 곰팡이 Drechslerella brochopaga Kan-23의 형태 및 계통분류)

  • Cho, Chun-Hwi;Kang, Doo-Sun;Kim, Yoon-Ji;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.44 no.1
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    • pp.63-68
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    • 2008
  • Strain Kan-23 was extracted from nematophagous fungi, which were isolated from the soil sample of oriental melon field. The strain exhibited the slow-growing characteristic forming conidia after prolonged incubation for 30 days. Morphological features of strain Kan-23 were observed under scanning electron microscope (SEM). It possesses erect conidiophores which contain $2{\sim}3$ side branches, with each branch producing $5{\sim}10$ conidia. The size of conidiophores were between $160{\sim}450\;{\mu}m$. Conidia were ellipsoidal with three septa[septum] in each conidium. Strain Kan-23 captured nematodes by means of giant constricting rings, which were observed in the glucose peptone agar medium. ITS region of rDNA sequence was analyzed. On the basis of the high sequence similarity of ITS region (99%), the Kan-23 strain was closely related to Drechslerella brochopaga (U51950). This is the first report on Drechslerella brochopaga as a nematophagous fungus in Korea.

Characteristics of a NP Strain for Cucumber mosaic virus(CMV-NP) Identified Newly from Sweet Pepper Showing Fruit Necrosis (과일 괴저 병징의 단 고추에서 분류동정한 오이모자이크바이러스의 새로운 계통 CMV-NP 특성)

  • Cho, Jeom-Deog;Kim, Jeong-Soo;Lee, Joong-Hwan;Chung, Bong-Nam
    • Research in Plant Disease
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    • v.14 no.2
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    • pp.134-137
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    • 2008
  • A strain of Cucumber mosaic virus(CMV) was isolated newly from sweet pepper(Capsicum annuum var. angulosum) showing necrosis with large necrotic spots on fruits and vein banding with malformation on leaf at Cheongdo area in Gyeongsangbukdo. The new strain was designated as a CMV-NP and the shape of virus particles was isometric of 26 nm in diameter from the sweet pepper fruit by Dip method. The strain of CMV-NP was identified genetically by VC/RT-PCR. CMV-NP could infect systemically on the 9 indicator plants including Cucumis sativus, but it could infect locally on Chenopodim amaranticolor and C. quinoa. CMV-NP induced the specific symptoms of necrotic rings on the inoculated and the upper leaves of N. rustica and Tetragonia expansa. On Cucumis sativus, the large chlorotic ring and vein chlorosis were produced on the upper leaves. CMV-NP had no virulence on Datura stramonium.

Ring-Rolling Design of a Large-Scale Ti-6Al-4V alloy (대형 Ti-6Al-4V 합금의 Ring-Rolling 공정설계)

  • Yeom, J.T.;Jung, E.J.;Kim, J.H.;Lee, D.G.;Park, N.K.;Choi, S.S.;Lee, C.S.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2006.05a
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    • pp.373-376
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    • 2006
  • The ring rolling design for a large-scale Ti-6Al-4V alloy ring was performed with a calculation method and FEM simulation. The ring rolling design includes geometry design and optimization of process variables. The calculation method was to determine geometry design such as initial billet and blank size, and final rolled ring shape. A commercial FEM code, SHAPE was used to simulate the effect of process variables in ring rolling on the distribution of the internal state variables such as strain, strain rate and temperature. In order to predict the forming defects during ring rolling, the process-map approach based on Ziegler's instability criterion was used with FEM simulation. Finally, an optimum process design to obtain sound Ti-6Al-4V rings without forming defects was suggested through combined approach of Ziegler's instability map and FEM simulation results.

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Process Design for Large-Scale Ring-Rolling of Ti-6Al-4V Alloy (Ti-6Al-4V 합금의 대형 링 압연공정설계)

  • Yeom, J.T.;Kim, J.H.;Lee, D.G.;Park, N.K.;Choi, S.S.;Lee, C.S.
    • Transactions of Materials Processing
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    • v.16 no.3 s.93
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    • pp.172-177
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    • 2007
  • The process design for large-scale ring rolling of Ti-6Al-4V alloy was performed by calculation method, processing map approach and FEM simulation. The ring rolling design includes geometry design and optimization of process variables. The calculation method was used to make geometry design such as initial billet and blank sizes, and final rolled ring shape. A commercial FEM code, SHAPE-RR was used to simulate the effect of process variables in ring rolling on the distribution of the internal state variables such as strain, strain rate and temperature. In order to predict the forming defects during ring rolling and the formation of over-heating above $\beta$-transus temperature due to deformation heating, the process-map approach based on Ziegler's instability criterion was used with FEM simulation. Finally, an optimum process design to obtain sound Ti-6Al-4V rings without forming defects was suggested through combined approach of Ziegler's instability map and FEM simulation results.

Elastic Analysis of Cold Extrusion Die Set with Stress Ring (보강링을 갖는 냉간 압출 금형 세트의 탄성해석)

  • 안성찬;이근안;김수영;임용택
    • Transactions of Materials Processing
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    • v.11 no.4
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    • pp.355-362
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    • 2002
  • In this study, an axi-symmetric finite element program for elastic analysis of the die set shrink fitted in cold extrusion was developed. The geometrical constraint according to shrink fit was enforced by employing the Lagrange multiplier method. The numerical results for strain and stress distributions in the die set including single and multi stress rings assembled by shrink fit were compared well with the Lame's equation for thick-walled solution available in the literature. To extend the applicability of the analysis program developed, various cases without or with stress ring and with pre-stress applied on stress ring were numerically investigated as well. This numerical approach enables the optimization study to determine optimal dimensions of die set to improve tool life for practical use in industry.

Computational and Experimental Investigation on U-type Seal of Hydraulic Actuator (유압액추에이터용 U자형 시일의 해석 및 실험적 고찰)

  • Yoo, Myung-Ho;Kwon, Jong-Ho;Lee, Taek-Sung
    • Journal of the Korean Society for Precision Engineering
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    • v.23 no.12 s.189
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    • pp.80-87
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    • 2006
  • Seals are usually made from elastomer, a kind of rubber, and it has the non-linearity and hyper-elasticity. U-type seals are used to prevent the leakage of internal fluid sealed in hydraulic actuator because they have more excellent performance than O-rings or rectangular seals. As a core part of hydraulic actuator, U-type seal gives much effect on performance and reliability of actuator. This study considers an NBR U-type seal under high pressure of a hydraulic actuator, and provides its deformation, stress-strain characteristic and contact force using the non-linear finite element analysis. Analysis results are compared with the experimental ones performed by the self-developed testing equipment. Verification result shows that this study presents a good application process for the effective design of U-type seals under high operation pressure.

Design of a Composite Flywheel Rotor for Energy Storage System (에너지 저장시스템용 복합재 플라이휠 로터의 설계)

  • 정희문;최상규;하성규
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.19 no.7
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    • pp.1665-1674
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    • 1995
  • An optimum design has been performed to maximize specific energy (SED) of composite flywheel rotor for energy storage system. The flywheel rotor is assumed to be an axisymmetric thick laminated shell with a plane strain state for structural analysis. For the structural analysis the centrifugal force is considered and the stiffness matrix equation was derived for each ring considering the interferences between the rings. The global stiffness matrix was derived by integrating the local stiffness matrix satisfying the conditions of force and displacement compatibilities. Displacements are then calculated from the global stiffness matrix and the stresses in each ring are also calculated. 3-D intra-laminar quadratic Tsai-Wu criterion is then used for the strength analysis. An optimum procedure is also developed to find the optimal interferences and lay up angle to maximize SED using the sensitivity analysis.

Development of Pressure Transducer and Measurement of Inter-Ring Gas Pressure in Internal Combustion Engine (압력 센서 개발 및 내연기관의 피스톤 링 사이 가스압력 측정)

  • 윤정의;이귀영
    • Transactions of the Korean Society of Automotive Engineers
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    • v.4 no.1
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    • pp.75-85
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    • 1996
  • The gas pressure acting on the rings in internal combustion engine influences the friction, wear and HC emission. In order to understand their characteristics, it is necessary to measure the interring gas pressure during engine operations. In this study, miniature type pressure transducer was developed to measure inter-ring gas pressure. And measurements of cylinder and inter-ring gas pressure were made on a gasoline engine running at full and part load conditions. Finally the characteristics of inter-ring gas pressure variation during engine operation were obtained form analysis of measured date.

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Optimized Design of O-ring Groove in LPG Filling Unit Using Taguchi Experimental Method (다구찌 실험법을 이용한 LPG 충전노즐 O-링 그루브의 최적화 설계연구)

  • Kim Chung-Kyun
    • Journal of the Korean Institute of Gas
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    • v.10 no.2 s.31
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    • pp.40-46
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    • 2006
  • In this paper, the optimized design of a rectangular O-ring groove has been analyzed for a maximum Cauchy stress and maximum strain using the Taguchi method. This method may efficiently optimize the design parameters for an O-ring groove of a LPG filling unit. The computed FEM results indicate that the optimized design parameters can only be drawn by nine experimental numbers of iterations when the Taguchi design technique has been employed with a finite element method. This means that the Taguchi design method is very useful for the optimization design of O-ring rectangular groove geometry. Based on the computed FEM results by the Taguchi design technique, the dimensions of a groove geometry are given as h=2.5 mm, d=2.74 mm, c=0.15 mm, and w=3.0 mm. In this study, the initial compression ratio of O-rings is recommended as 8.7% for a gas supply pressure of 18 $kg/cm^2$.

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Investigation of Siderophore production and Antifungal activity against Phytophthora capsici as related to Iron (III) nutrition by Lysobacter antibioticus HS124

  • Ko, Hyun-Sun;Tindwa, Hamisi;Jin, Rong De;Lee, Yong-Seong;Hong, Seong-Hyun;Hyun, Hae-Nam;Nam, Yi;Kim, Kil-Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.4
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    • pp.650-656
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    • 2011
  • Lysobacter antibioticus HS124 isolated from pepper rhizosphere soil produced catechol type siderophore. Purified siderophore by Diaion HP-20 and silica gel column chromatography showed several hydroxyl functional groups adjacent to benzene rings by analysis of $^1H$ NMR spectroscopy. The strain HS124 showed different activities to suppress Phytophthora capsici with different concentrations of exogenous Fe (III) in minimal medium where antifungal activity with $100{\mu}M$ Fe (III) was approximately 1.5 times higher than in absence of Fe (III). Bacterial population in this Fe (III)-amended medium was also highest with $8.9{\times}10^8\;CFU\;ml^{-1}$ which also corresponded to the strongest siderophore activity. When grown in rich medium (minimal medium with N, $P_2O_5K_2O$ and glucose), HS124 exhibited approximately 2 times stronger antifungal activity compared to minimal medium. In pot trials, treatments of bacterial culture grown in rich medium with (C1) or without (C2) $100{\mu}M$ Fe (III) exhibited a high protection of pepper plants from disease, compared to medium only with (M1) or without (M2) $100{\mu}M$ Fe (III). Especially, treatment C1 showed the best disease control effect of about 70 %. Thus, the strain HS124 should be recommended as a potential biocontrol agent against P. capsici in pepper.