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Morphological and Phylogenetic Characteristics of a Nematophagous Fungus, Drechslerella brochopaga Kan-23  

Cho, Chun-Hwi (KAFCO Biochemistry Research Institute)
Kang, Doo-Sun (KAFCO Biochemistry Research Institute)
Kim, Yoon-Ji (Institute of Microbial Ecology and Resources, Mokwon University)
Whang, Kyung-Sook (Institute of Microbial Ecology and Resources, Mokwon University)
Publication Information
Korean Journal of Microbiology / v.44, no.1, 2008 , pp. 63-68 More about this Journal
Abstract
Strain Kan-23 was extracted from nematophagous fungi, which were isolated from the soil sample of oriental melon field. The strain exhibited the slow-growing characteristic forming conidia after prolonged incubation for 30 days. Morphological features of strain Kan-23 were observed under scanning electron microscope (SEM). It possesses erect conidiophores which contain $2{\sim}3$ side branches, with each branch producing $5{\sim}10$ conidia. The size of conidiophores were between $160{\sim}450\;{\mu}m$. Conidia were ellipsoidal with three septa[septum] in each conidium. Strain Kan-23 captured nematodes by means of giant constricting rings, which were observed in the glucose peptone agar medium. ITS region of rDNA sequence was analyzed. On the basis of the high sequence similarity of ITS region (99%), the Kan-23 strain was closely related to Drechslerella brochopaga (U51950). This is the first report on Drechslerella brochopaga as a nematophagous fungus in Korea.
Keywords
Drechslerella brochopaga; giant constricting ring; GPA medium; nematophagous fungi; rDNA ITS;
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