• Mycobiology
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• 제50권5호
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• pp.374-381
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• 2022

In the mating of filamentous basidiomycetes, dikaryotic mycelia are generated through the reciprocal movement of nuclei to a monokaryotic cytoplasm where a nucleus of compatible mating type resides, resulting in the establishment of two different dikaryotic strains having the same nuclei but different mitochondria. To better understand the role of mitochondria in mushrooms, we created four sets of dikaryotic strains of Lentinula edodes, including B2×E13 (B2 side) and B2×E13 (E13 side), B5×E13 (B5 side) and B5×E13 (E13 side), E8×H3 (E8 side) and E8×H3 (H3 side), and K3×H3 (K3 side) and K3×H3 (H3 side). The karyotypes and mitochondrial types of the dikaryotic strains were successfully identified by the A mating type markers and the mitochondrial variable length tandem repeat markers, respectively. Comparative analyses of the dikaryotic strains on the mycelial growth, substrate browning, fruiting characteristics, and mitochondrial gene expression revealed that certain mitochondria are more effective in the mycelial growth and the production of fruiting body, possibly through the activated energy metabolism. Our findings indicate that mitochondria affect the physiology of dikaryotic strains having the same nuclear information and therefore a selection strategy aimed at mitochondrial function is needed in the development of new mushroom strain.

• 한국분말재료학회지
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• 제30권2호
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• pp.146-155
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• 2023

The Ti-6Al-4V lattice structure is widely used in the aerospace industry owing to its high specific strength, specific stiffness, and energy absorption. The quality, performance, and surface roughness of the additively manufactured parts are significantly dependent on various process parameters. Therefore, it is important to study process parameter optimization for relative density and surface roughness control. Here, the part density and surface roughness are examined according to the hatching space, laser power, and scan rotation during laser-powder bed fusion (LPBF), and the optimal process parameters for LPBF are investigated. It has high density and low surface roughness in the specific process parameter ranges of hatching space (0.06-0.12 mm), laser power (225-325 W), and scan rotation (15°). In addition, to investigate the compressive behavior of the lattice structure, a finite element analysis is performed based on the homogenization method. Finite element analysis using the homogenization method indicates that the number of elements decreases from 437,710 to 27 and the analysis time decreases from 3,360 to 9 s. In addition, to verify the reliability of this method, stress-strain data from the compression test and analysis are compared.

• Genomics & Informatics
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• 제21권1호
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• pp.7.1-7.11
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• 2023

The gram-positive bacterium Listeria monocytogenes is an important foodborne intracellular pathogen that is widespread in the environment. The functions of hypothetical proteins (HP) from various pathogenic bacteria have been successfully annotated using a variety of bioinformatics strategies. In this study, a HP Imo0888 (NP_464414.1) from the Listeria monocytogenes EGD-e strain was annotated using several bioinformatics tools. Various techniques, including CELLO, PSORTb, and SOSUIGramN, identified the candidate protein as cytoplasmic. Domain and motif analysis revealed that the target protein is a PemK/MazF-like toxin protein of the type II toxin-antitoxin system (TAS) which was consistent with BLASTp analysis. Through secondary structure analysis, we found the random coil to be the most frequent. The Alpha Fold 2 Protein Structure Prediction Database was used to determine the three-dimensional (3D) structure of the HP using the template structure of a type II TAS PemK/MazF family toxin protein (DB ID_AFDB: A0A4B9HQB9) with 99.1% sequence identity. Various quality evaluation tools, such as PROCHECK, ERRAT, Verify 3D, and QMEAN were used to validate the 3D structure. Following the YASARA energy minimization method, the target protein's 3D structure became more stable. The active site of the developed 3D structure was determined by the CASTp server. Most pathogens that harbor TAS create a crucial risk to human health. Our aim to annotate the HP Imo088 found in Listeria could offer a chance to understand bacterial pathogenicity and identify a number of potential targets for drug development.

• Coupled systems mechanics
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• 제12권3호
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• pp.199-220
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• 2023

This article presents a new analytical model to study the effect of porosity on the shear correction factors (SCFs) of functionally graded porous beams (FGPB). For this analysis, uneven and logarithmic-uneven porosity functions are adopted to be distributed through the thickness of the FGP beams. Critical to the application of this theory is a determination of the correction factor, which appears as a coefficient in the expression for the transverse shear stress resultant; to compensate for the assumption that the shear strain is uniform through the depth of the cross-section. Using the energy equivalence principle, a general expression is derived from the static SCFs in FGPB. The resulting expression is consistent with the variationally derived results of Reissner's analysis when the latter are reduced from the two-dimensional case (plate) to the one-dimensional one (beam). A convenient algebraic form of the solution is presented and new study cases are given to illustrate the applicability of the present formulation. Numerical results are presented to illustrate the effect of the porosity distribution on the (SCFs) for various FGPBs. Further, the law of changing the mechanical properties of FG beams without porosity and the SCFare numerically validated by comparison with some available results.

• 한국식생활문화학회지
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• 제24권5호
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• pp.540-551
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• 2009

This study was conducted to investigate the quality characteristics of mungbean starch gels containing various hydrocolloids (carrageenan, locust bean gum and xanthan gum) during room temperature storage ($25^{\circ}C$ for 24, 48 and 72 hours). Carrageenan and xanthan gum reduced the pasting viscosity of mungbean starch, whereas the locust bean gum increased the viscosity. The melting characteristics, as assessed by DSC, showed that carrageenan and xanthan gum delayed gelatinization of mungbean starch and the locust bean gum had no effect on this property. The lightness (L) of the gels with the locust bean gum was similar to that without the additive during storage, whereas that with carrageenan and xanthan gum was higher than that without the additive. Hardness, chewiness and gumminess of the gels with the locust bean gum was higher than that without the additive during storage, whereas that with carrageenan and xanthan gum was lower than that without the additive. The rupture stress, rupture strain and rupture energy of the gels with carrageenan and xanthan gum was lower than that without the additive during storage, whereas that with the locust bean gum was similar to that without the additive. In the sensory evaluation, springiness and cohesiveness of the gels with carrageenan and xanthan gum were lower than those without the additive, whereas springiness, brittleness and hardness of the gels with the locust bean gum were higher than those without the additive. In addition, the overall acceptability of the gels with the locust bean gum improved. The above results showed that carrageenan and xanthan gum lowered the quality characteristics of the mungbean starch gel and the locust bean gum improved them. Thus, the addition of 0.5% locust bean gum is an appropriate method for improving the quality characteristics of mungbean starch gel.

• Steel and Composite Structures
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• 제46권6호
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• pp.839-856
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• 2023

This work presents a simple four-unknown refined integral plate theory for mechanical and thermal buckling behaviors of functionally graded (FG) plates resting on Visco-Pasternak foundations. The proposed refined high order shear deformation theory has a new displacement field which includes indeterminate integral variables and contains only four unknowns in which any shear correction factor not used, with even less than the conventional theory of first shear strain (FSDT). Governing equations are deduced from the principle of minimum total potential energy and a Navier type analytical solution is adopted for simply supported FG plates. The Visco-Pasternak foundations is considered by adding the impact of damping to the usual foundation model which characterized by the linear Winkler's modulus and Pasternak's foundation modulus. The accuracy of the present model is demonstrated by comparing the computed results with those available in the literature. Some numerical results are presented to show the impact of material index, elastic foundation type, and damping coefficient of the foundation, on the mechanical and thermal buckling behaviors of FG plates.

• 대한토목학회논문집
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• 제28권4A호
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• pp.517-528
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• 2008

이 논문에서는 철근과 콘크리트 사이의 부착-슬립을 실제적으로 고려한 철근콘크리트 접합부의 이력 손상 모델을 제안하였다. 슬립을 가시화하기 위해 콘크리트와 철근의 변위장이 서로 다른 프레임 요소를 개발하였다. 파이버 단면 개념으로부터 콘크리트, 철근 그리고 부착에 대한 적합방정식을 정의하였다. 부분적인 제하 및 재재하 상태를 고려하기 위해 철근 이력곡선의 수정이 이루어졌다. 단조증가 상태의 국부적 부착응력-슬립 관계는 손상 계수에 따라 슬립이 역전될 때마다 갱신하였다. 구속된 콘크리트에 매입된 철근 시험체와 기초에 정착된 철근콘크리트 기둥 시험체, 그리고 보-기둥 부재의 수치해석을 통해 모델의 정확성을 검증하였고, 부착-슬립 효과를 고려함으로써 하중 이력에 따른 에너지 소산 정도를 평가할 수 있었다.

• 대한토목학회논문집
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• 제26권6A호
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• pp.935-941
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• 2006

지진과 같은 동적반복하중을 받은 강구조물은 정적하중시와 다른 이력거동을 보인다. 이는 동적변형중인 구조용 강재는 정적상태와 다른 역학적 특성 및 응력-변형률 관계를 보이기 때문이다. 즉, 지진하중을 받는 원형 강기둥과 같은 강구조물의 이력거동을 정확히 예측하기 위해서는 정동적 변형 상태를 가정한 재하속도에 따른 대상구조물 내하력 및 변형의 차이점을 명확히 파악해야 한다. 이에 본 연구에서는 저자에 의해 제안된 SM490강재의 동적 반복소성모델과 이를 적용한 3차원 탄소성 유한요소해석을 이용하여 재하속도와 지름-두께(D/t)비를 변수로한 SM490 원형강기동의 동적해석을 수행하였다. 해석 결과를 통하여 정적에서 동적변형상태로 재하속도 변화에 따른 SM490 원형강기둥의 이력거동 특성 즉, 내하력 및 에너지소산효율의 변화를 명확히 파악하였다.

• 한국지형학회지
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• 제27권1호
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• pp.33-45
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• 2020

A number of unconsolidated deposits, consisting of a layer of gravels and silt, are found in Dangjeong-ri, Seocheon-gun in the western coast. From below in the stratigraphic sequence, the gravel layer ranging up to a maximum thickness of about 2 meters is interpreted as being formed by fluvial processes of an old channel (Dangjeong S.), and the overlying silt or sandy silt layer of 2 to 3 thickness meters is assumed to be emerged paleo-tidal sediments which was deposited in low tidal-energy environments. As the results of rock surface IRSL datings, the depositional ages of gravels are confirmed as ca. 78,000 ~ 83,000 years BP, indicating that the layer was formed in response to a high-stand sea level of MIS 5a along the Dangjeongcheon estuary. It is presumed that the relative height of 4.5 meter between the altitude of the stream bed (9.5 m) and the altitude of the bedrock boundary in the gravel layer (14 m) indicates the uplift amount since deposition. Paleo-sedimentary environments and an altitude of paleo-shoreline in the study area will be discussed with additional age dating focused on the silt layer.

• Parasites, Hosts and Diseases
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• 제61권4호
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• pp.439-448
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• 2023

Tick infestation causes a significant threat to human and animal health, requiring effective immunological control methods. This study aimed to investigate the potential of recombinant Haemaphysalis longicornis enolase protein for tick vaccine development. The exact mechanism of the recently identified enolase protein from the H. longicornis Jeju strain remains poorly understood. Enolase plays a crucial role in glycolysis, the metabolic process that converts glucose into energy, and is essential for the motility, adhesion, invasion, growth, and differentiation of ticks. In this study, mice were immunized with recombinant enolase, and polyclonal antibodies were generated. Western blot analysis confirmed the specific recognition of enolase by the antiserum. The effects of immunization on tick feeding and attachment were assessed. Adult ticks attached to the recombinant enolase-immunized mice demonstrated longer attachment time, increased bloodsucking abilities, and lower engorgement weight than the controls. The nymphs and larvae had a reduced attachment rate and low engorgement rate compared to the controls. Mice immunized with recombinant enolase expressed in Escherichia coli displayed 90% efficacy in preventing tick infestation. The glycolytic nature of enolase and its involvement in crucial physiological processes makes it an attractive target for disrupting tick survival and disease transmission. Polyclonal antibodies recognize enolase and significantly reduce attachment rates, tick feeding, and engorgement. Our findings indicate that recombinant enolase may be a valuable vaccine candidate for H. longicornis infection in experimental murine model.