• Microbiology and Biotechnology Letters
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• v.47 no.1
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• pp.25-33
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• 2019

Lactobacillus rhamnosus BFE5264, isolated from a Maasai fermented milk product ("kule naoto"), was previously shown to exhibit bile acid resistance, cholesterol assimilation, and adhesion to HT29-MTX cells in vitro. In this study, we re-annotated and analyzed the previously reported complete genome sequence of strain BFE5264. The genome consists of a circular chromosome of 3,086,152 bp and a putative plasmid, which is the largest one identified among L. rhamnosus strains. Among the 2,883 predicted protein-coding genes, those with carbohydrate-related functions were the most abundant. Genome analysis of strain BFE5264 revealed two consecutive CRISPR regions and no known virulence factors or antimicrobial resistance genes. In addition, previously known highly variable regions in the genomes of L. rhamnosus strains were also evident in strain BFE5264. Pairwise comparison with the most studied probiotic strain L. rhamnosus GG revealed strain BFE5264-specific deletions, probably due to insertion sequence-mediated recombination. The latter was associated with loss of the spaCBA pilin gene cluster and exopolysaccharide biosynthetic genes. Comparative genomic analysis of the sequences from all available L. rhamnosus strains revealed that they were clustered into two groups, being within the same species boundary based on the average nucleotide identities. Strain BFE5264 had a sister group relationship with the group that contained strain GG, but neither ANI-based hierarchical clustering nor core-gene-based phylogenetic tree construction showed a clear distinctive pattern associated with the isolation source, implying that the genotype alone cannot account for their ecological niches. These results provide insights into the probiotic mechanisms of strain BFE5264 at the genomic level.

• Coupled systems mechanics
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• v.9 no.4
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• pp.381-396
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• 2020

Analytical investigation of the fracture of inhomogeneous beam with two parallel lengthwise cracks is performed. The thickness of the beam varies continuously along the beam length. The beam is loaded in three-point bending. Two beam configurations with different lengths of the cracks are analyzed. The two cracks are located arbitrary along the thickness of the beam. Solutions to the strain energy release rate are derived assuming that the material has non-linear elastic mechanical behavior. Besides, the beam exhibits continuous material inhomogeneity along its thickness. The balance of the energy is analyzed in order to derive the strain energy release rate. Verifications of the solutions are carried-out by considering the complementary strain energy stored in the beam configurations. The influence of the continuous variation of the thickness along the beam length on the lengthwise fracture behavior is investigated. The dependence of the lengthwise fracture on the lengths of the two parallel cracks is also studied.

• Proceedings of the Korean Geotechical Society Conference
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• 2000.03b
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• pp.183-190
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• 2000

Effective range of Hydraulic Hammer Compaction was studied by numerical analysis instead of empirical method. Numerical analyses were carried out with commercial FEM code, ABAQUS, and verified by comparing the numerical results with field tests of Hydraulic Hammer Compaction. Most of material properties were evaluated by data from laboratory and in-situ tests. Vertical effective range was estimated by distribution curve of plastic strain energy dissipated through soil layers under dynamic load and these results were in good agreement with field tests. Based on verification, the effects of governing properties of Hydraulic Hammer Compaction such as number of hit can be determined by numerical analyses. In addition, vertical effective range can also be determined by Menard's empirical equation using the external work at converging time of plastic strain energy in numerical analysis. This implies that the minimum energy of Hydraulic Hammer Compaction for improvement can be determined by Menard's equation.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2007.05a
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• pp.311-314
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• 2007

The experimental method of measuring dynamic properties of structures was presented. The method is based on the flexural wave propagation characteristics. Using the method, change in structural dynamic properties due to damage is measured. The crack has much more significant impact on the strain energy than the inertial effects. From this, the sensitivity of the dynamic stiffness on the crack location is estimated by calculating the strain energy. When the wave propagates, the strain and kinetic energies shows cyclic changed over space. The crack that occurred at locations where the wave energy is in the form of the potential energy affected most significantly the wave propagation characteristics. The effects of crack location on the wave propagation were used to determine the crack location.

• Structural Engineering and Mechanics
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• v.56 no.1
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• pp.107-121
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• 2015

The main objective of this research is to present the procedures of combining topology, shape & sizing optimization for truss structure by employing strain energy as objective function under the constraints of volume fractions which yield more general solution than that of total weight approach. Genetic Algorithm (GA) is used as searching engine for the convergence solution. A number of algorithms from previous research are used for evaluating the feasibility and stability of candidate to accelerate convergence and reduce the computational effort. It is followed by solving problem for topology & shape optimization and topology, shape & sizing optimization of truss structure to illustrate the feasibility of applying the objective function of strain energy throughout optimization stages.

• Steel and Composite Structures
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• v.34 no.5
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• pp.671-679
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• 2020

The new energy-based criterion, named Reinforcement Strain Energy Density (ReiSED), is proposed to investigate the fracture behavior of the cracked orthotropic materials in which the crack is embedded in the matrix along the fibers. ReiSED is an extension of the well-known minimum strain energy density criterion. The concept of the reinforced isotropic solid as an advantageous model is the basis of the proposed mixed-mode I/II criterion. This model introduces fibers as reinforcements of the isotropic matrix in orthotropic materials. The effects of fibers are qualified by defining reinforcement coefficients at tension and shear modes. These coefficients, called Reduced Stress (ReSt), provide the possibility of encompassing the fiber fraction in a fracture criterion for the first time. Comparing ReiSED fracture limit curve with experimental data proves the high efficiency of this criterion to predict the fracture behavior of orthotropic materials.

• Transactions of The Korea Fluid Power Systems Society
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• v.4 no.4
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• pp.21-27
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• 2007

A hydraulic breaker for construction machinery generally used for the destroying and disassembling of buildings, crashing road pavement, breaking rocks at quarry and so on. So the measurement of the impact energy of a hydraulic breaker is very important thing to prove its capability to manufacturers and customers. Therefore the prediction of impact energy in design process is very helpful to the most of breaker manufacturers. In this study, we carried on modeling and simulation of a hydraulic breaker to predict impact energy via commercial CAE software. The modeling and simulation of a hydraulic breaker was achieved with two parts. One is a hydraulic circuit analysis part via AMESim and the other is impact strain analysis part via ANSYS.

• Coupled systems mechanics
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• v.11 no.6
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• pp.543-556
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• 2022

Delamination of multilayered inhomogeneous beam that exhibits non-linear relaxation behavior is analyzed in the present paper. The layers are inhomogeneous in the thickness direction. The dealamination crack is located symmetrically with respect to the mid-span. The relaxation is treated by applying a non-linear stress-straintime constitutive relation. The material properties which are involved in the constitutive relation are distributed continuously along the thickness direction of the layer. The delamination is analyzed by applying the J-integral approach. A time-dependent solution to the J-integral that accounts for the non-linear relaxation behavior is derived. The delamination is studied also in terms of the time-dependent strain energy release rate. The balance of the energy is analyzed in order to obtain a non-linear time-dependent solution to the strain energy release rate. The fact that the strain energy release rate is identical with the J-integral value proves the correctness of the non-linear solutions derived in the present paper. The variation of the J-integral value with time due to the non-linear relaxation behavior is evaluated by applying the solution derived.

• Steel and Composite Structures
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• v.36 no.6
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• pp.703-710
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• 2020

This paper presents numerical and theoretical investigations on the strain rate in steel-concrete composite (SC) panels under low-velocity impact of a hemispherical rigid body. Finite element analyses were performed on five specimens with different loading rates. The impact energy was kept constant to eliminate its influence by simultaneously altering the velocity and mass of the projectile. Results show that the strain rate in most parts of the specimens was low and its influence on bearing capacity and energy dissipation was limited in an average sense of space and time. Therefore, the strain rate effect can be ignored for the analyses of global deformation. However, the strain rate effect should be considered in local contact problems. Equations of the local strain and strain rate were theoretically derived.

• Journal of Ocean Engineering and Technology
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• v.27 no.2
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• pp.19-26
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• 2013

Stress triaxiality is recognized as one of the most important factors for predicting the failure strain of ductile metals. This study dealt with the effect of the average stress triaxiality on the failure strain of a typical low-temperature high-strength marine structural steel, EH36. Tensile tests were carried out on flat specimens with different notches, from relatively smooth to very sharp levels. Numerical simulations of each specimen were performed by using ABAQUS. The failure initiation points in numerical simulations were identified from a comparison of the engineering stress vs. strain curves obtained from experiments with simulated ones. The failure strain curves for various dimensionless critical energy levels were established in the average stress triaxiality domain and compared with the identified failure strain points. It was observed that most of the failure initiation points were approximated with a 100% dimensionless critical energy curve. It was concluded that the failure strains were well expressed as a function of the average stress triaxiality.