• The Microorganisms and Industry
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• v.19 no.1
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• pp.8-17
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• 1993

젖산생성균의 .betha.-gal의 생성과 Bif. longum KCTC 3215에 의한 .betha.-gal 생산, 정제 및 특성에 관한 연구와 젖산생성균의 .betha.-gal 유전자의 클로닝 및 대장균에의 발현과 Str.thermophilus SKD 1006의 Lac Z 유전자를 비교 분석하였다.

• Journal of Korean Neurosurgical Society
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• v.58 no.3
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• pp.248-253
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• 2015

Objective : The treatment of giant cell tumor (GCT) is mainly performed surgically. However, GCT in spine seems difficult to treat because of the limited surgical accessibility and proximity. In this report, we analyzed the outcome of GCT treatment in spine. Methods : Between 2000 and 2012, 19 patients received treatment for GCT in spine. Median age at their first diagnosis was 31 years, 10 patients were male, and 9 female. Fourteen tumors were located in the sacrum, 1 in cervical, 1 in thoracic and 3 in lumbar spine. As primary treatment, gross total removal (GTR) was done in 6 patients, and subtotal removal (STR) in 13 patients. Radiation therapy (RT) as an adjuvant therapy was performed in 2 cases in GTR group and 10 cases in STR group. Results : During the follow-up, 7 patients had local recurrence (36.8%). The average period until recurrence after primary treatment was 14 months. No recurrence was detected in GTR group. Recurrence was noted in 7 out of 13 patients who underwent STR. These differences were statistically significant (p=0.024). A median of recurrence free period (RFP) was 84 months. Also average RFP of the RT group was 112 months, and non-RT group was 65 months. These differences were statistically significant (p=0.041). Conclusion : Treatment of choice for GCT in spine is a complete removal of tumor without neurological deficits. In case of incomplete removal, radiation therapy may be a useful adjuvant treatment modality.

• Journal of Astronomy and Space Sciences
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• v.15 no.1
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• pp.101-110
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• 1998

We calculated line profiles of 32 Cyg with an assumed $str{\"{o}}mgren$ sphere at orbital phases 0.06 and 0.78. The wind models with $Alfv\'{e}n$ waves show diminished line profiles compared to those of the models using power velocity laws. The reduced density of absorbers in HII region produce weak profiles, but line profiles at ${\phi}=0.06$ do not show the differences except in the red edge of absorption. At ${\phi}=0.78$ however, we could reproduce the line profiles of Alfven waves model by the power law models with two velocity gradients. It suggests that the power law model with 2 acceleration regions could reduce the errors in the theoretical line formation with no consideration of wind acceleration mechanism.nism.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.2
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• pp.431-436
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• 2009

Home network is a rapidly growing area as new technologies are emerging, and new applications are being developed. The progress of home network technologies is growing the home network management technology for control and management of the digital appliances. Embodiment of these home network show marked ich have use for complicated and diversifiable processing. Design of home network system does very important stage through home network comes essential pars. In this paper, We designed and constitute Home network system which designs module using embedded system, STR710F Chip and CC2420 RF Module-Chip with the intention for using RS232C and USB.

• 한국해양학회지
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• v.5 no.2
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• pp.65-72
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• 1970

The effect of fresh water and various concentrations of aged seawater on the survival of fecal pollution bacteria, Escherichia coli, type I, Aerobacter aerogenes type I, and Streptococcus faecalis type were determined. Survivals of bacteria were measured by the membrane silter technique. Three species of bacteria indicate more tolerance in fresh water than in seawater. After 14-day incubation in fresh water, survival rates of bacteria were 90% withe E.coli, 20% with A.aerogenes, and 0.6% with Str.faecalis. However, the survival rate of fecal pollution bacteria decreases as the concetnration of seawater is increased. Generally, the death rate of E.coli is least affected by concentration of seawater. A.aerogenes is eliminated more rapidly with higher concentration of seawater, while Str.faecalis marks rather slight variation of elimination in various concentrations of seawater. In 100- percent seawater (Cl 18.1 ), the days required for 99.9% elimination of bacteria were 4.5 days with A.aerogenes, and 6.5 days with E.coli and Str.faecalis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.10
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• pp.6715-6721
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• 2015

Recently, DNA data of missing person, killed person, and missing child continue to increase but most of statistical calculation for paternity confirmation is being done through manual methods or Excel. Therefore, we need development of a software which is able to facilitate both systematic management and effective analysis of Short Tandem Repeat (STR) derived from DNA data. Without extensive testing, through a twenty-month study was developed a web-based system which performs paternity analysis and kinship analysis easily based on the various options. The former uses an existing algorithm for paternity index and the latter does Identity by descent (IBD) formula. Due to our system validated over real datasets in terms of likelihood ratio and probability of paternity, it ensures increased reliability as well as effective management and analysis of DNA data in mass disaster. In addition, it includes advanced features such as an integrated environment, user-centered interface, process automation and so on.

• Korean Journal of Veterinary Research
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• v.40 no.1
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• pp.187-195
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• 2000

The ejaculates from 67 HanWoo prove bull, bred in Livestock Improvement Main center of NLCF, were used to determine the correlation between the sperm motional characteristics and the pregnancy rate of artificial insemination(AI). The motional characteristics of sperm were analysed by Computer-assisted sperm analyser(CASA), thereafter inseminated equally 1,256 heads of cow regarding to parity, age, and live weight. There were no significant difference(p>0.05) in the pregnancy rate according to year from 1996 to 1998, but the LIN, ALH, STR, BCF, MAD and WOB of sperm in the year 1997, were highest pregnancy rate, were higher than those of sperm in the year 1998, were lowest pregnancy rate(p<0.05). The semen had no significant effect on pregnancy rate according to season(p>0.05). However spring, had a little higher pregnancy rate than that of autumn, were higher than autumn in VSL, VAP, LIN, ALH, BCF, MAD and WOB, but in DNM. The pregnancy rates of spring in the year 1996 and 1997 were higher than that of autumn in the year 1998(p<0.05). The spring in the year 1997, highest in pregnancy rate, were higher than the autumn in the year 1998 in VSL, VAP, LIN, STR, BCF, MAD and WOB, but in DNM(p<0.05). There were no the motion characteristic of sperm that was significant correlate with pregnancy rate of AI as the semen were analysed before artificial insemination and those, had some degree characteristics in motility, viability and abnormality, were used to AI. However there were a tendency that the higher the VSL, VAP, ALH, LIN, STR, BCF, MAD and WOB and the lower the DNM were, the higher the pregnancy rate of AI were.

• Analytical Science and Technology
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• v.12 no.3
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• pp.260-264
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• 1999

A simple and rapid procedure, called FoLT-PCR(Formamide Low Temperature-Polymerase Chain Reaction) was applied to amplifying DNA directly from various forensic biological evidences including human blood, saliva, hair root, or semen without any DNA preparative steps. We added washing step with non-ionic detergent, 1% Triton X-100, and used Taq DNA polymerase instead of Tth DNA polymerase to amplify 3 STR loci and gender allele simultaneouly. Optimal concentration of formamide and annealing temperature were determined empirically to 8%(v/v), and $48^{\circ}C$ respectively. We also compared this method with standard PCR.

• Journal of Food Hygiene and Safety
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• v.5 no.1
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• pp.27-34
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• 1990

As a link in the studies on the extracellular polysaccharide by lactic acid bacteria, the experiment was conducted to investigate the viscosity variations of 10% reconstituted skim milk and optimum cultural conditions for the production of extracellular polysaccharide. 1. The viscosity of 10% reconstituted skim milk cultured by 8tr. thermophilu8 510 reached 5,000 CP at $41^{\circ}C$ and on 5 days. Because it was the highest value among the microorganisms tested, Str. thermophilu8 510 was chosen as a main test microoganism and studied on factors affecting maximum viscosity depending upon production of exopolysaccharide. 2. Absorbance of synthetic culture medium was highest at $41^{\circ}C$. Sucrose, in a member of carbohydrates, was the most effective carbon source for the production of exopolysaccharide within 5% concentration. 3. The production of exopplysaccharide was stimulated by $Mg^{++}$.

• Animal cells and systems
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• v.5 no.3
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• pp.237-241
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• 2001

Three STR loci (STRX1[AGAT]$_{n}$, HPRTB[AGAT]$_{n}$ and ARA[AGC]$_{n}$) on X chromosome and three other STR loci (DYS390[CTG(A)T]$_{n}$, DYS392[ATT]$_{n}$ and DYS39［GATA]$_{n}$) on Y chromosome were analyzed in 154 unrelated healthy Korean subjects. Four loci (STRX1, HPRTB, DYS390 and DYS393) were amplified by quadruplex polymerase chain reaction (PCR) using fluorescent labeled primers (FLP). ARA and DYS392 were amplified separately using single PCR, similarly by using FLP. They were then run in an automated DNA sequencer and were analyzed with Genescan software. We found 7 alleles (308-332 bp) in STRX1, 7 alleles (275-299 bp) in HPRTB, 16 alleles (252-315 bp) in ARA, 6 alleles (203-223 bp) in DYS390, 7 alleles (245-263bp) in DYS392 and 5 alleles (116-132 bp) in DYS393. The ＊13(34%), ＊13(5l%), ＊23 (l8%), ＊23(50%), ＊14(39%) and ＊13(40%) alleles were observed to be the highest frequencies of STRX1, HPRTB, ARA, DYS390, DYS392 and DYS393, respectively. The detection of STR loci on sex chromosomes by quadruplex PCR allows simple determination of sex and identification of an individual. individual.