• Title/Summary/Keyword: Storage duration

Search Result 341, Processing Time 0.03 seconds

Evaluation of the stability of IgM and specific antibody response of sevenband grouper Epinephelus septemfasciatus for application of antibody-detection ELISA (항체검출 ELISA 적용을 위한 능성어 IgM의 안정성 및 특이 항체 반응 평가)

  • Kim, Chun-Seob;Jang, Min-Seok;Kim, Wi-Sik;Kim, Jong-Oh;Kim, Du-Woon;Kim, Do-Hyung;Han, Hyun-Ja;Jeong, Sung-Ju;Oh, Myung-Joo
    • Journal of fish pathology
    • /
    • v.22 no.3
    • /
    • pp.335-342
    • /
    • 2009
  • The stability of immunoglobulin M (IgM) on different serum storage conditions and specific antibody response were tested using the serum collected from sevenband grouper Epinephelus septemfasciatus by enzyme-linked immunosorbent assay (ELISA). To test the effect of storage temperature and duration, sevenband grouper antiserum against bovine serum albumin (BSA) was stored at -80, -20 or 4${^{\circ}C}$ for 1, 34, 61 or 119 days. In addition, to test the effect of repeated freeze-thawing condition, the anti-BSA fish serum was frozen at -20 and -80${^{\circ}C}$ and then thawn and frozen for 1, 5 or 10 times repeatedly. Consequently, no significant difference was found in ELISA optical density (O.D.) values of sera for the above mentioned storage conditions: different temperatures (-80, -20 and 4${^{\circ}C}$), durations of storage (1, 34, 61 and 119 days), and repeated thaw-freeze cycles (1, 5, and 10 times), indicating that IgMs of test fish were stable. The specific antibody response of sevenband grouper was observed after BSA-immunization of the test fish reared at 20 ${^{\circ}C}$ or 25${^{\circ}C}$. At the rearing temperature of 20${^{\circ}C}$, the specific antibody against BSA first appeared at 14 days and maximum antibody titer was observed between 21 and 28 days, while at the rearing temperature of 25 ${^{\circ}C}$, specific antibody appeared at 7 days and maximum antibody titer was observed between 14 and 21 days. In conclusion, the rearing temperature at 25${^{\circ}C}$ gave a faster and higher specific antibody response than at 20${^{\circ}C}$ and the specific antibody response maintained for approximately 2 months at 20℃ and 25${^{\circ}C}$.

Activation Analysis of Dual-purpose Metal Cask After the End of Design Lifetime for Decommission (설계수명 이후 해체를 위한 금속 겸용용기의 방사화 특성 평가)

  • Kim, Tae-Man;Ku, Ji-Young;Dho, Ho-Seog;Cho, Chun-Hyung;Ko, Jae-Hun
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
    • /
    • v.14 no.4
    • /
    • pp.343-356
    • /
    • 2016
  • The Korea Radioactive Waste Agency (KORAD) has developed a dual-purpose metal cask for the dry storage of spent nuclear fuel that has been generated by domestic light-water reactors. The metal cask was designed in compliance with international and domestic technology standards, and safety was the most important consideration in developing the design. It was designed to maintain its integrity for 50 years in terms of major safety factors. The metal cask ensures the minimization of waste generated by maintenance activities during the storage period as well as the safe management of the waste. An activation evaluation of the main body, which includes internal and external components of metal casks whose design lifetime has expired, provides quantitative data on their radioactive inventory. The radioactive inventory of the main body and the components of the metal cask were calculated by applying the MCNP5 ORIGEN-2 evaluation system and by considering each component's chemical composition, neutron flux distribution, and reaction rate, as well as the duration of neutron irradiation during the storage period. The evaluation results revealed that 10 years after the end of the cask's design life, $^{60}Co$ had greater radioactivity than other nuclides among the metal materials. In the case of the neutron shield, nuclides that emit high-energy gamma rays such as $^{28}Al$ and $^{24}Na$ had greater radioactivity immediately after the design lifetime. However, their radioactivity level became negligible after six months due to their short half-life. The surface exposure dose rates of the canister and the main body of the metal cask from which the spent nuclear fuel had been removed with expiration of the design lifetime were determined to be at very low levels, and the radiation exposure doses to which radiation workers were subjected during the decommissioning process appeared to be at insignificant levels. The evaluations of this study strongly suggest that the nuclide inventory of a spent nuclear fuel metal cask can be utilized as basic data when decommissioning of a metal cask is planned, for example, for the development of a decommissioning plan, the determination of a decommissioning method, the estimation of radiation exposure to workers engaged in decommissioning operations, the management/reuse of radioactive wastes, etc.

Planning for Securing Instreamflow of Gapcheon Stream in Daejeon (대전 갑천의 유지유량 확보 방안)

  • Noh, Jae-Kyoung
    • Korean Journal of Agricultural Science
    • /
    • v.36 no.1
    • /
    • pp.87-98
    • /
    • 2009
  • The objective of this study is to evaluate the effect of increasing instream flow at Gapcheon stream of Daejeon city by considering two virtual reservoirs upstream, respectively; Geum-gok reservoir and Koe-gok reservoir upstream, respectively. The paralleled and cascaded reservoir operations were performed including the existing Jang-an and Bang-dong reservoirs. The results are summarized as follows. Firstly, from the Bang-dong and Geum-gok cascaded reservoir's water balance analysis, instream flow of $6.83Mm^3$ was able to be supplied to downstream, and water supply indexes of Geum-gok reservoir were analyzed to have the rate of water supply divided by watershed area of 403.4 mm, the rate of water supply divided by rainfall of 33.0 %, the rate of water supply divided by inflow of 96.4 %, the rate of water supply divided by storage capacity of 81.9 %, and the rate of inflow divided by storage capacity of 112.3 %. Secondly, from the Jang-an and Geum-gok paralleled reservoir's water balance analysis, flow durations at Gapcheon station were analyzed to have Q95 (the 95th high flow) of $4.806m^3/s$, Q185 (the 185th high flow) of $2.217m^3/s$, Q275 (the 275th high flow) of $1.140m^3/s$, and Q355 (the 355th high flow) of $0.887m^3/s$. Thirdly, inflow to Koe-gok reservoir was simulated including the Jang-an and Bang-dong paralleled reservoir's water balance analysis, instream flow of $49.60Mm^3$ was able to be supplied from Koe-gok reservoir to downstream, and water supply indexes of Koe-gok reservoir were analyzed to have the rate of water supply divided by watershed area of 246.5 mm, the rate of water supply divided by rainfall of 19.4 %, the rate of water supply divided by inflow of 40.8 %, the rate of water supply divided by storage capacity of 412.1 %, and the rate of inflow divided by storage capacity of 1,189.8 %. Fourthly, daily streamflows at Gapcheon stream were simulated including outflows from Koe-gok reservoir, flow durations at Gapcheon station were analyzed to have Q95 (the 95th high flow) of $4.501m^3/s$, Q185 (the 185th high flow) of $2.277m^3/s$, Q275 (the 275th high flow) of $1.743m^3/s$, and Q355 (the 355th high flow) of $1.564m^3/s$. The conclusion appeared that the effect of increasing instream flow at Gapcheon stream from Koe-gok reservoir was more higher than that from Geum-gok reservoir.

  • PDF

Factors Influencing on the Drop of in vitro Protein Digestibility in Dried Fish Meat (건어육(乾魚肉) 저장(貯藏)중의 단백질(蛋白質) 소화율(消化率) 저하요인(低下要因))

  • Kim, Sang-Ae;Lee, Kang-Ho;Ryu, Hong-Soo
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.15 no.1
    • /
    • pp.45-55
    • /
    • 1986
  • This paper aims to study the reactions of lipid or oxidized lipid with protein during drying and storing hair tail fish(Trichurus lepturus) and flounder(Kanakius kitaharai) being generally consumed as dried seafood products in Korea and their influence on the drop of in vitro protein digestibility of these fish meat. The results of the study are as follows: The digestibility of the raw materials of flounder and hair tail fish was 87.63% and 86.08% respectively, and that of sundried and hot air dried materials went down $1{\sim}2$ percent with drying process. But in case of defatted and sundried materials, the rate increased 85.15% and 87.15% respectivley. After 30 days of storage, the digestibility decreased in all materials, and hot air dried meat showed a significant decrease. Trypsin indigestible substrate (TIS) contents of flounder and hair tail fish, in case of raw materials were 0.88 and 0.96mg/g. solid repectiveiy and in case of defatted and sundried materials, TIS contents showed a low increase and digestibility showed a high increase. Brown pigment formation had a wide range of increase in case of the sundried and hot air dried materials and it was increased with duration of storage and temperature. The major fatty acids in the fats of hair tail fish and flounder were $C_{18:1},\;C_{16:0},\;C_{22:6}\;and\;C_{16:1}$ and rate of unsaturated to saturated fatty acids was 79.2:20.8 for flounder, 67.8:32.2 for hair tail fish. After 30 days of storage at room temperature. saturated fatty acids increased compared with the raw materials while unsaturated fatty acids showed a tendency to decrease. Avaialble lysine of hair tail fish was higher than that of flounder and both of them lost about 8.23% of that in raw materials after 30 days of storage.

  • PDF

Development and Reliability of Intraoral Appliance for Diagnosis and Control of Bruxism (이갈이 진단 및 조절용 구내장치의 개발과 신뢰도 조사)

  • Kim, Seung-Won;Kim, Mee-Eun;Kim, Ki-Suk
    • Journal of Oral Medicine and Pain
    • /
    • v.30 no.1
    • /
    • pp.69-77
    • /
    • 2005
  • The purposes of this study were to develop and introduce a novel intraoral appliance for bruxism composed of power switch and biofeedback device and further to examine inter- and intra-reliability of the appliance prior to clinical tests. The newly-developed appliance consisted of detection sensors, a central processing unit (CPU), a reactor and a storage unit and a displayer. Compact-sized, waterproof switches were selected as bruxism detection sensor and any sensor activation by clenching or grinding event was processed at the CPU and transmitted, by radio wave, to the reactor and storage unit and triggered auditory or vibratory signal, subsequently producing biofeedback to the patient with bruxism. The data on bruxing event in the storage unit can be displayed on the computer, making it possible analyzing frequency, duration and nature of bruxism. Cast models were obtained from ten volunteers with normal occlusion to evaluate reliability of the appliances. For inter-operator reliability on the intraoral appliances, each operator of the two fabricated the appliance for the same subject and compared the minimal contact forces provoking auditory biofeedback reaction in vertical, lateral and central directions. Intra-operator reliability was also investigated on the appliances made by a single operator at two separate times with an interval of two days. Conclusively, the newly-developed appliance is compact and safe to use in oral circumstance and easy to make. Furthermore, it had to be proven reliability excellent enough to apply in clinical settings. Thus, it is assumed that this appliance with the processor and the storage of data and auditory or vibratory biofeedback function is available and useful to analyze and control bruxism.

Water shortage assessment by applying future climate change for boryeong dam using SWAT (SWAT을 이용한 기후변화에 따른 보령댐의 물부족 평가)

  • Kim, Won Jin;Jung, Chung Gil;Kim, Jin Uk;Kim, Seong Joon
    • Journal of Korea Water Resources Association
    • /
    • v.51 no.12
    • /
    • pp.1195-1205
    • /
    • 2018
  • In the study, the water shortage of Boryeong Dam watershed ($163.6km^2$) was evaluated under future climate change scenario. The Soil and Water Assessment Tool (SWAT) was used considering future dam release derived from multiple linear regression (MLR) analysis. The SWAT was calibrated and verified by using daily observed dam inflow and storage for 12 years (2005 to 2016) with average Nash-Sutcliffe efficiency of 0.59 and 0.91 respectively. The monthly dam release by 12 years MLR showed coefficient of determination ($R^2$) of above 0.57. Among the 27 RCP 4.5 scenarios and 26 RCP 8.5 scenarios of GCM (General Circulation Model), the RCP 8.5 BCC-CSM1-1-M scenario was selected as future extreme drought scenario by analyzing SPI severity, duration, and the longest dry period. The scenario showed -23.6% change of yearly dam storage, and big changes of -34.0% and -24.1% for spring and winter dam storage during 2037~2047 period comparing with 2007~2016 period. Based on Runs theory of analyzing severity and magnitude, the future frequency of 5 to 10 years increased from 3 in 2007~2016 to 5 in 2037~2046 period. When considering the future shortened water shortage return period and the big decreases of winter and spring dam storage, a new dam operation rule from autumn is necessary for future possible water shortage condition.

Operation rule curve for supplying urban instream flow from reservoir (도시 하천유지유량 공급의 저수지 운영 방법)

  • Noh, Jae-Kyoung;Lee, Jae-Nam
    • Korean Journal of Agricultural Science
    • /
    • v.38 no.1
    • /
    • pp.163-172
    • /
    • 2011
  • To provide the operation rule curve for suppling instream flow to urban stream from reservoir, the Soho reservoir with watershed area of 7.4 $km^2$ and total water storage of 2.58 $Mm^3$ was planned at the headwaters of the Daejeoncheon. Daily streamflow was simulated and using the simulated streamflow and desired instream flow, the operation rule curve by Senga method was drawn and evaluated through reservoir operation. Senga method is derived by accumulating the differences between streamflow and desired instream flow adversely. Water storages were simulated on a daily basis to supply urban instream flow from Soho reservoir, but the amount of supplying instream flow to urban stream was not nearly increased comparing with that of normal operation that does not used the rule curve. Thereafter the new simulation-based operation rule curve was derived and applied to supply instream flow from Soho reservoir. In normal operation, the amount of instream flow was shown to 15,000 $m^3$/d, but it was increased to 27,700 $m^3$/d in withdrawal limited operation using the new derived rule curve, in which the applicability of this rule curve was proved. Also comparing with the flow duration curves at station just before urban Daejeoncheon stream without and with upstream Soho reservoir, the 95th flow was decreased from 1.64 mm/d to 1.51 mm/d, and the 355th flow was increased from 0.17 mm/d to 0.30 mm/d. Monthly streamflows during October to March were increased from 10.6~24.1 mm to 24.1~34.0 mm with the increasing rate of 141~227%.

Effect of Low Dose γ Radiation on the Dormancy Breaking and Growth of in vitro Microtubers of Potato (Solanum tuberosum L.) Stored at Low Temperature (저선량 방사선이 저온 저장한 감자 기내 소괴경의 휴면타파와 생육에 미치는 효과)

  • Kim, Jae-Sung;Kim, Dong-Hee;Back, Myung-Hwa;Jeon, Jae-Heung;Lee, Young-Bok
    • Horticultural Science & Technology
    • /
    • v.19 no.4
    • /
    • pp.515-520
    • /
    • 2001
  • To observe the stimulating effect of low dose ${\gamma}$ radiation on the dormancy breaking and growth, microtubers of two potato cultivars (Solanum tuberosum L. cv. Dejima and cv. Superior) were irradiated at the dose of 0.5-30 Gy. Though it varied with cultivars and storage duration, sprouting rate, plant growth and tuber yield were promoted by 2-8 Gy irradiation in microtuber of 'Dejima' stored at low temperature. On the other hand, in microtuber of 'Superior', sprouting rate was promoted by 2 and 4 Gy irradiation, and the growth and tuber yield by 4 Gy irradiation. These results suggest that low dose of ${\gamma}$ radiation could have stimulating effects on the dormancy breaking of microtuber and potato growth.

  • PDF

Classification of Online Tracking Technology and Implications in User Perspective (온라인 트래킹 기술 분류 및 이용자 관점에서의 시사점)

  • Lee, Bohan;Rha, Jong-Youn
    • Journal of Digital Convergence
    • /
    • v.16 no.9
    • /
    • pp.159-172
    • /
    • 2018
  • This study searched and analyzed online tracking technologies. It tried to understand what to consider when establishing policies related to online tracking. Online tracking technologies were classified into 'general cookies', 'super cookies', 'fingerprinting', 'device ID tracking' and 'cross-device tracking'. Political considerations should include the layers of online tracking, the subjects of tracking technology, purpose of use, duration and storage format of information, and development of technology. The implications of this study are as follows: first, policy makers and industry should be aware that the degree of risk perceived by users may vary according to the characteristics of online tracking technology. Secondly, it is necessary to understand factors that affect the classification of online tracking technology. Finally, in the industry, preemptive measures such as building an integrated privacy system are needed to relieve anxiety of users and to build trust.

Design and Implementation of Dermatology $CO_2$ Laser System (피부과용 $CO_2$레이저시스템의 설계 및 구현)

  • Kim, Whi-Young
    • Journal of the Korea Society of Computer and Information
    • /
    • v.6 no.2
    • /
    • pp.8-13
    • /
    • 2001
  • We demonstrate a pulsed CO$_2$laser with long pulse duration of millisecond order in the low pressure less than 30 Torr. A new power supply for our laser system switches the voltage of AC power line(60㎐) directly. The power supply doesn't need elements such as a rectified bridge, energy-storage capacitors. and a current-limiting resistor in the discharge circuit. In order to control the laser output power, the pulse repetition rate is adjusted up to 60㎐ and the firing angle of SCR gate is varied from 30˚ to 150˚. A ZCS(Zero Crossing Switch) circuit and a PIC one-chip microprocessor are used to control the gate signal of SCR precisely. The maximum laser output is 23W at the total pressure of 18 Torr, the pulse repetition rate of 60㎐, and SCR gate firing angle of 90˚. In addition, the obtained laser pulse width is approximately 3㎳(FWHM)