• Title/Summary/Keyword: Storage and accumulation

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Effects of Sulfur Nutritional Forms on Accumulation of Seed Storage Proteins in Soybean (Glycine max)

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    • Korean Journal of Plant Resources
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    • v.10 no.3
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    • pp.221-226
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    • 1997
  • Improvement of seed protein quality might be an essential issus in soybean and would give more profit directly to both farmers and users. This study was carried out to investigate the effects of reduced-S form(s) on seed storage protein components in soybean during seed filling stages. The reduced-S forms during seed fill were sodium thiosulfate, sodium sulfite, sodium sulfide, thioaceteat, $\beta$-mercaptoethanol, thiourea, thiamine-HCI, L-cysteine, L-cystine, and L-methionine. Seed storage protein concentration did not appear to be affected by any reduced-S forms. However, glycinin and $\beta$-conglycinin concentration seemed to be changed greatly by L-methionine. This resulted in the increase in the 11S/7S ratio(3.58). Among the $\beta$-conglycinin, $\beta$-subunit was not accumulated at all. $\alpha$-subunit concentration appeared to be decreased and $\alpha'$-subunit concentration was not altered in comparison with sulfate control. Also, $\beta$-conglycine concentration, especially $\beta$-subunit concentration, tended to be decreased with L-cystine treatment, resulting in an increase in the 11S/7S ratio(1.83). The glycinin concentration tended to be increased at the expense of the decrease in the $\beta$-conglycinin concentration. Therefore, it is suggested that enhancing soybean protein quality would be achieved by improving metabolic pathways of S assimilation in soybean plants during seed filling period under sulfate-sufficient condition.

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Biomass and Carbon Storage Pattern in Natural and Plantation Forest Ecosystem of Chhattisgarh, India

  • Jhariya, Manoj Kumar;Yadav, Dhiraj Kumar
    • Journal of Forest and Environmental Science
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    • v.34 no.1
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    • pp.1-11
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    • 2018
  • We studied natural and plantation forest ecosystem of Sarguja in Chhattisgarh, India in order to understand how vegetation biomass, carbon stock and its allocation patterns vary among the sites. For this, stratified random sampling was opted to measure the different layers of vegetation. Wide floral diversity was found in the natural forest site as compared to the teak stand. Overall, 17 tree species found in natural forest comprising 8 families while in the teak stand 6 species were recorded. In understory strata 23 species were recorded (18 herbs and 5 shrubs) in natural forest whereas in teak stand 20 herb species and 3 shrubs were found. Great variation was also seen in the population dynamics of the different vegetation stratum in concerned sites. The sapling, seedling and herb density was found to be highest in natural stand while tree and shrub density was more in teak stand. Results indicated that stand biomass of the natural site was $321.19t\;ha^{-1}$ while in the teak stand it was $276.61t\;ha^{-1}$. The total biomass of tree layer in plantation site was $245.22t\;ha^{-1}$ and natural forest $241.44t\;ha^{-1}$. The sapling, seedling, shrub and forest floor biomass was found highest under natural forest as compared to the teak plantation site. Carbon stock has similar trend as that of biomass accumulation in natural forest and teak stand. Higher biomass accumulation and carbon stock were recorded in the higher girth class gradation of the population structure. Proper efforts are required to manage these diverse ecosystems to obtain higher biomass and sustainable ecological services.

Inhibition of GM3 Synthase Attenuates Neuropathology of Niemann-Pick Disease Type C by Affecting Sphingolipid Metabolism

  • Lee, Hyun;Lee, Jong Kil;Bae, Yong Chul;Yang, Song Hyun;Okino, Nozomu;Schuchman, Edward H.;Yamashita, Tadashi;Bae, Jae-Sung;Jin, Hee Kyung
    • Molecules and Cells
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    • v.37 no.2
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    • pp.161-171
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    • 2014
  • In several lysosomal storage disorders, including Niemann-Pick disease Type C (NP-C), sphingolipids, including glycosphingolipids, particularly gangliosides, are the predominant storage materials in the brain, raising the possibility that accumulation of these lipids may be involved in the NP-C neurodegenerative process. However, correlation of these accumulations and NP-C neuropathology has not been fully characterized. Here we derived NP-C mice with complete and partial deletion of the Siat9 (encoding GM3 synthase) gene in order to investigate the role of ganglioside in NP-C pathogenesis. According to our results, NP-C mice with homozygotic deletion of GM3 synthase exhibited an enhanced neuropathological phenotype and died significantly earlier than NP-C mice. Notably, in contrast to complete depletion, NP-C mice with partial deletion of the GM3 synthase gene showed ameliorated NP-C neuropathology, including motor disability, demyelination, and abnormal accumulation of cholesterol and sphingolipids. These findings indicate the crucial role of GM3 synthesis in the NP-C phenotype and progression of CNS pathologic abnormality, suggesting that well-controlled inhibition of GM3 synthesis could be used as a therapeutic strategy.

Terminal Dilation and Transformation of the Protein-filled ER to Form Protein Bodies in Pea (Pisum sativum L. var, exzellenz) Cotyledons (완두 자엽에서 소포체 말단의 팽창에 의한 단백과립 발달)

  • Jeong, Byung-Kap
    • Applied Microscopy
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    • v.29 no.4
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    • pp.499-509
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    • 1999
  • Accumulations of the storage proteins in protein storage vacuole and the differentiation of protein bodies from protein-filled ER in developing pea cotyledons have been investigated using conventional and immunoelectron microscopy. To improve the fixation quality, single cells separated enzymatically from sliced cotyledons were used. At early stages of seed development osmiophilic protein accumulates in rER lumen were observed quite often. This protein-filled ER cisternae were differentiated into cytoplasmic protein bodies at late stage by the process called terminal dilations which have been considered a principal route of the formation of cytoplasmic protein bodies somewhat later in seed maturation. Immunocytochemical labellings of the vicilin and legumin show that presence of vicilin on both of the cytoplasmic PB and PD, but limited presence of legumin only on the cytoplasmic PB at intermediate stage of seed development. Immunogold labellings of Bip, ER retention protein, were observed on the inner periphery of protein deposits in protein storage vacuole. This result was regarded that Bip can recognize and retrieve misfolded protein during active accumulation of storage protein to the PD in PSV.

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Waste Disposal Models for Manufacturing Firm and Disposal Firm

  • Tsai, Chi-Yang;Nagaraj, Sugarla Edwin
    • Industrial Engineering and Management Systems
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    • v.10 no.2
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    • pp.115-122
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    • 2011
  • This research considers a system containing a manufacturing firm who generates waste material during manufacturing process, and a disposal firm who collects and disposes the waste material. Identification of the optimal number of pick ups and the amount of waste to be disposed at certain period of time in terms of cost minimization is studied. Two types of waste accumulation rates, constant and linearly increasing, are discussed and mathematical models are developed. It can be shown that the results for these two different types of waste accumulation differ in a wide range because of the difference in the way of how waste is accumulated, which disturbs the storage cost. An integrated model is also developed and discussed in which both the manufacturing firm and the disposal firm benefit from the coordination between the two parties. It is shown that the optimal policy adopted by the integrated approach can provide a strong and consistent cost-minimizing effect for both the manufacturing firm and the disposal firm over the existing approach. Finally, all the models are verified by a numerical example and the results are compared.

Biomass, Primary Nutrient and Carbon Stock in a Sub-Himalayan Forest of West Bengal, India

  • Shukla, Gopal;Chakravarty, Sumit
    • Journal of Forest and Environmental Science
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    • v.34 no.1
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    • pp.12-23
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    • 2018
  • Quantitative information on biomass and available nutrients are essential for developing sustainable forest management strategies to regulate atmospheric carbon. An attempt was made at Chilapatta Reserve Forest in Duars region of West Bengal to quantify its above and below ground carbon along with available "N", "P" and "K" in the soil. Stratified random nested quadrats were marked for soil, biomass and litter sampling. Indirect or non-destructive procedures were employed for biomass estimation. The amount of these available nutrients and organic carbon quantified in soil indicates that the forest soil is high in organic carbon and available "K" and medium in phosphorus and nitrogen. The biomass, soil carbon and total carbon (soil C+C in plant biomass) in the forest was 1,995.98, 75.83 and $973.65Mg\;ha^{-1}$. More than 90% of the carbon accumulated in the forest was contributed by the trees. The annual litter production of the forest was $5.37Mg\;ha^{-1}$. Carbon accumulation is intricately linked with site quality factors. The estimated biomass of $1,995.98Mg{\cdot}ha^{-1}$ clearly indicates this. The site quality factor i.e. tropical moist deciduous with optimum availability of soil nutrients, heavy precipitation, high mean monthly relative humidity and optimum temperature range supported luxuriant growth which was realized as higher biomass accumulation and hence higher carbon accumulated.

Differential Diagnosis of Thick Myocardium according to Histologic Features Revealed by Multiparametric Cardiac Magnetic Resonance Imaging

  • Min Jae Cha;Cherry Kim;Chan Ho Park;Yoo Jin Hong;Jae Min Shin;Tae Hoon Kim;Yoon Jin Cha;Chul Hwan Park
    • Korean Journal of Radiology
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    • v.23 no.6
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    • pp.581-597
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    • 2022
  • Left ventricular (LV) wall thickening, or LV hypertrophy (LVH), is common and occurs in diverse conditions including hypertrophic cardiomyopathy (HCM), hypertensive heart disease, aortic valve stenosis, lysosomal storage disorders, cardiac amyloidosis, mitochondrial cardiomyopathy, sarcoidosis and athlete's heart. Cardiac magnetic resonance (CMR) imaging provides various tissue contrasts and characteristics that reflect histological changes in the myocardium, such as cellular hypertrophy, cardiomyocyte disarray, interstitial fibrosis, extracellular accumulation of insoluble proteins, intracellular accumulation of fat, and intracellular vacuolar changes. Therefore, CMR imaging may be beneficial in establishing a differential diagnosis of LVH. Although various diseases share LV wall thickening as a common feature, the histologic changes that underscore each disease are distinct. This review focuses on CMR multiparametric myocardial analysis, which may provide clues for the differentiation of thickened myocardium based on the histologic features of HCM and its phenocopies.

Assessing Organic Matter and Organic Carbon Contents in Soils of Created Mitigation Wetlands in Virginia

  • Ahn, Changwoo;Jones, Stacy
    • Environmental Engineering Research
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    • v.18 no.3
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    • pp.151-156
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    • 2013
  • Several soil properties were studied from three young created mitigation wetlands (<10 years old), which were hydrologically comparable in the Piedmont region of Virginia. The properties included soil organic matter (SOM), soil organic carbon (SOC), pH, gravimetric soil moisture, and bulk density ($D_b$). No significant differences were found in the soil properties between the wetlands, except SOM and SOC. SOM and SOC indicated a slight increase with wetland age; the increase was more evident with SOC. Only about a half of SOC variability found in the wetlands was explained by SOM ($R^2$ = 0.499, p < 0.05). The majority of the ratios of SOM to SOC for these silt-loam soils ranged from 2.0 to 3.5, which was higher than the 1.724 Van Bemmelen factor, commonly applied for the conversion of SOM into SOC in estimating the carbon storage or accumulation capacity of wetlands. The results may caution the use of the conversion factor, which may lead to an overestimation of carbon sequestration potentials of newly created wetlands. SOC, but not SOM, was also correlated to $D_b$, which indicates soil compaction typical of most created wetlands that might limit vegetation growth and biomass production, eventually affecting carbon accumulation in the created wetlands.

Formation of Aflatoxins on Stored Grain Contaminated with Aspergillus flavus (Aspergillus flavus로 오염(汚染)된 저장곡류(貯藏穀類)에서의 Aflatoxin 생성(生成))

  • Lee, Kwan-Young;Kim, Young-Bae;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.7 no.1
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    • pp.7-10
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    • 1975
  • A series of experiment was undertaken to pursue the possibility of aflatoxin accumulation in stored grains which were artificially contaminated with A. flavus var. columnaris isolated from deteriorated rice in Korea. In storage of rice grain, aflatoxin formation needed relative humidity over 80%, once accumulated aflatoxin was degraded by other microbial growth maintaining 50% level and the absence of other microoganisms brought about the remarkable accumulation of aflatoxin. The formation of aflatoxin in grains was increasing in the order of soybean, peanut, corn, wheat, barley, foxtail millet, rice, polished wheat, mung bean and great millet and it appears to be affected by the size and oil content of grains.

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Effects of Various Calcium Salt Spray on Calcium Accumulation into Apple Fruits (Malus domestica Borkh.) (칼슘급원별 수관살포가 사과 과실의 칼슘축적에 미치는 영향)

  • Choe, Jong-Seung
    • The Journal of Natural Sciences
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    • v.6 no.1
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    • pp.49-54
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    • 1993
  • This study was conducted to investigate the influence of various calcium salts on the accumulation of calcium in apple fruits when sprayed on whole tree. Differences in the total calcium contents of fruits were found between calcium sources and cultivars. In 'Tsugaru' , calcium nitrate, calcium chloride, and calcium carbonate were all effective but only calcium chloride and calcium carbonate appeared to be effective in 'Fuji' . Major parts of the applied calcium were accumulated in the feel and outer flesh. Ethylene evolution of fruit was retarded during storage with the increase of total calcium content in 'Fuji' fruit treated calcium acetate.

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