Proceedings of the Plant Resources Society of Korea Conference
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2010.05a
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pp.14-14
/
2010
Plastid proteomics are essential organelles present in virtually all cells in plants and green algae. Plastids are responsible for the synthesis and storage of key molecules required for the basic architecture and functions of plant cells. The proteome of plastid, and in particular of chloroplast, have received significant amounts of attention in recent years. Various fractionation and mass spectrometry (MS) techniques have been applied to catalogue the chloroplast proteome and its sub-organelles compartments. To better understanding the function of the lumenal sub-organelles within the thylakoid network, we have carried out a systematical analysis and identification of the lumenal proteins in the thylakoid of wheat by using Tricine-SDS-PAGE, and LTQ-ESI-FTICR mass spectrometry followed by SWISS-PROT database searching. We isolation and fractionation these membrane from fully developed wheat leaves using a combination of differential and gradient centrifugation couple to high speed ultra-centrifuge. After collecting all proteins to eliminate possible same proteins, we estimated that there are 407 different proteins including chloroplast, chloroplast stroma, lumenal, and thylakoid membrane proteins excluding 20 proteins, which were identified in nucleus, cytoplasm and mitochondria. A combination of these three programs (PSORT, TargetP, TMHMM, and TOPPRED) was found to provide a useful tool for evaluating chloroplast localization, transit peptide, transmembranes, and also could reveal possible alternative processing sites and dual targeting. Finally, we report also sub-cellular location specific protein interaction network using Cytoscape software, which provides further insight into the biochemical pathways of photosynthesis. The present work helps understanding photosynthesis process in wheat at the molecular level and provides a new overview of the biochemical machinery of the thylakoid in wheat.
Journal of the Institute of Electronics Engineers of Korea SD
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v.46
no.7
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pp.29-38
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2009
In this paper, a high performance pipelined computing design of parallel multiplier-temporal buffer-parallel accumulator is present for the convolution-based non-cascaded architecture aiming at the real time Discrete Wavelet Transform(DWT) processing. The convolved multiplication of DWT would be reduced upto 1/4 by utilizing the filter coefficients symmetry and the up/down sampling; and it could be dealt with 3-5 times faster computation by LUT-based DA multiplication of multiple filter coefficients parallelized for product terms with an image data. Further, the reutilization of computed product terms could be achieved by storing in the temporal buffer, which yields the saving of computation as well as dynamic power by 50%. The convolved product terms of image data and filter coefficients are realigned and stored in the temporal buffer for the accumulated addition. Then, the buffer management of parallel aligned storage is carried out for the high speed sequential retrieval of parallel accumulations. The convolved computation is pipelined with parallel multiplier-temporal buffer-parallel accumulation in which the parallelization of temporal buffer and accumulator is optimize, with respect to the performance of parallel DA multiplier, to improve the pipelining performance. The proposed architecture is back-end designed with 0.18um library, which verifies the 30fps throughput of SVGA(800$\times$600) images at 90MHz.
Recently, the National Emergency Management Agency of Korea has been operating the National Disaster Management System. Nevertheless, there are numerous difficulties in systematic controlling the steep slope DB promptly, because the system's functions in input and control for steep slope information are merely simple. Futhermore, the hazard degrees of steep slope lands nowadays have risen suddenly in accordance with the increase rate of large scale landslides such as the landslide cases of Umyeonmountain, Chuncheon province and others or sever rain storm cases. these lead to rapid increases in frequencies of nature disasters nationally. therefore, it is needed to develop the GIS-based integrated management system for steep slope information in order to manage disasters in advance or high-degree control. This study shows the national GIS-based integrated management system to prevent the disasters that caused by steep slope lands. The integrated management system developed in this study consists of surface information input modules, realtime DB liaison modules of integrated underground information, V-world background map-based GIS, integrated management system for steep slope information user modules, realtime liaison interface modules designed for utilizing steep slope information. Also, tests about stability of data storage, system stability and consistency of processing speed were performed.
Journal of the Korea Academia-Industrial cooperation Society
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v.19
no.6
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pp.46-54
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2018
The Internet of Things (IoT) is an environment in which various devices provide services to users through communications. Because of the nature of the IoT, data are stored and distributed in heterogeneous information systems. In this situation, IoT end applications should be able to access data without having information on where the data are or what the type of storage is. This mechanism is called Service Discovery (SD). However, some problems arise, since the current SD architectures search for data in physical devices. First, turnaround time increases from searching for services based on physical location. Second, there is a need for a data structure to manage devices and services separately. These increase the administrator's service configuration complexity. As a result, the device-oriented SD structure is not suitable to the IoT. Therefore, we propose an SD structure called Name-based Service-centric Service Discovery (NSSD). NSSD provides name-based centralized SD and uses the IoT edge gateway as a cache server to speed up service discovery. Simulation results show that NSSD provides about twice the improvement in average turnaround time, compared to existing domain name system and distributed hash table SD architectures.
The ontology has been gaining increasing interests by recent arise of the semantic web and related technologies. The focus is mostly on inference query processing that requires high-level techniques for storage and searching ontologies efficiently, and it has been actively studied in the area of semantic-based searching. W3C's recommendation is to use RDFS and OWL for representing ontologies. However memory-based editors, inference engines, and triple storages all store ontology as a simple set of triplets. Naturally the performance is limited, especially when a large-scale ontology needs to be processed. A variety of researches on proposing algorithms for efficient inference query processing has been conducted, and many of them are based on using proven relational database technology. However, none of them had been successful in obtaining the complete set of inference results which reflects the five characteristics of the ontology properties. In this paper, we propose a new index structure called hyper cube index to efficiently process inference queries. Our approach is based on an intuition that an index can speed up the query processing when extensive inferencing is required.
Fast developments of broadcasting technologies since 1990s enabled not only High Definition Television service providing high quality audiovisual contents at home but also mobile broadcasting service providing audiovisual contents to high speed moving vehicle. Terrestrial Digital Multimedia Broadcasting (T-DMB) is one of the technologies developed for mobile broadcasting service, which has been successfully commercialized. One of the major technical breakthroughs achieved by T-DMB in addition to robust vehicular reception is an adoption of framework based on MPEG-4 System. It naturally enables integrated interactive data services by using Binary Format for Scene (BIFS) technology for scene description and representation of graphics object and Object Descriptor Framework representing multimedia service components as objects. T-DMB interactive data service has two fundamental limitations. Firstly, graphic data for interactive service should be always overlaid on top of a video not to be rendered out of it. Secondly, data for interactive service is only received by broadcasting channel. These limitations were considered as general in broadcasting systems. However, they are being considered as hard limitations for personalized data services using location information and user characteristics which are becoming widely used for data services of smart devices in these days. In this paper, the architecture of T-DMB hybrid data service is proposed which is utilizing broadcasting network, wireless internet and local storage for delivering BIFS data to overcome these limitations. This paper also presents hybrid BIFS technology to implement T-DMB hybrid data service while maintaining backward compatibility with legacy T-DMB players.
Highways are characterized by high non-point pollutant emissions due to high traffic volumes and sections that cause abrupt change in driving speed (i.e. rest stations, ticketing office, etc.). Most highways in Korea were constructed with layers that do not allow adequate infiltration. Moreover, non-point pollution reduction facilities were not commonly installed on domestic highways. This study was conducted to evaluate a facility treating highway runoff and develop a cost-effective design for infiltration facilities by using soil amendment techniques. Performing soil amendment increased the hydraulic retention time (HRT) and infiltration rate in the facility by approximately 30% and 20%, respectively. The facility's efficiency of removing non-point pollutants (Total Suspend Soiled (TSS), Chemical Oxygen Demand(COD), Biological Oxygen Demand(BOD), Total Nitrogen (TN) and Total Phosphorus, (TP) were also increased by 20%. Performing soil amendment on areas with low permeability can increase the infiltration rates by improving the storage volume capacity, HRT, and infiltration area. The application of infiltration facilities on areas with low permeability should comply with the guidelines presented in the Ministry of Environment's Standards for installation of non-point pollution reduction facilities. However, soil amendment may be necessary if the soil infiltration rate is less than 13 mm/hr.
Precooked powder of redbean porridge (RP) was prepared by the series of process extrusion, drying, milling and blending with a mixture of whole redbean flour and corn starch and others. The optimum process and quality of products for RP were investigated. After extrusion under the moisture content 24 to 26%, twin screw speed 350 rpm, extrusion temperature 150 to $155^{\circ}C$ and feed rate 60 kg/hr, the product had a higher quality with its natural redbean flavor/color. During the extrusion process, extrusion temperature and specific mechanical energy increased from 150 to $198^{\circ}C$ and from 134 to 144 kwh/ton respectively, as the amount of addition water decreased from 6 to 2 kg/hr. By the hot air drying of redbean extrudate (RE). it could be dried below to 4% moisture content, of which level considered as an optimal moisture content for anti-caking of the powdered product, at $80^{\circ}C$ for 4hrs and at $100^{\circ}C$ for 1.5 hrs respectively. In the sieve analysis of extrudate powder, when the product milled through a mesh size of 0.5 mm or 1.0 mm, about 80% or 65% of the feed was passed a 65 mesh screen respectively. Moisture absorption of final blended products was formed a cake under 100% of relative humidity after 13 hrs of storage. As the amount of RE powder reduced, the flavor score of products decreased by sensory evaluation of products prepared by the different ratio of RE powder, corn starch and sugar.
Iron objects become corroded at fast speed from the moment when they are excavated, so it is needed to control corrosion through processes of conservation treatment. However, re-corrosion mostly takes place in excavate iron objects, although they have already gone through the process of conservation treatment, and it is more difficult to carry out the second conservation treatment of re-corroded excavated iron objects than the first conservation treatment, and it requires a longer period of time to treat them as well. In this study, aims to discover factors of re-corrosion by scientifically analyzing corrosion products generated during the process of storage after the process of conservation treatment. The finished on conservation treatment of the iron artifacts, which were unearthed from three ancient site in Gyeongju by using the same conservation method between 2002 and 2009, re-corrosion condition observed on the packaging-iron artifacts. Focused on 9 target forged iron artifacts among them, this study analyzed the physical changes by mass measurement, naked-eye and microscopic observations and the chemical changes by SEM-EDS, XRD, IC and ICP analysis. The results show that the yellowish brown corrosion products formed on the facing surface of part dropped from the artifacts had different associated forms but acicular shape. In addition, the acicular shape became clearer as the color changed from red to yellowish brown. According to the process when the conservation treatment was completed, the mass of the artifacts increased in proportion to the corrosion products and the chloride ion ($Cl^-$) concentration had a tendency to increase relatively. ${\beta}$-FeOOH (akaganeite) was confirmed in the XRD analysis for the corrosion products of all the collected samples. As a result of ICP analysis, $Na^+$ and $Ca^{2+}$ components were confirmed.
The purpose of this study was to evaluate the shear bond strength of three light-cured glass ionomer cements to blood contaminated bovine dentin. The materials used in this study were Fuji II LC, Dyract and Variglass VLC. The dentin conditioners were 10% polyacrylic acid, 10% maleic acid and 10% phosphoric acid. 180 lower anterior bovine teeth were selected in this study. The teeth were embedded in acrylic resin and were grounded with 320 to 600 grit silicon carbide paper to create a flat dentin surface. The teeth were divided into SIX groups. The experimental procedures in six groups were as follows; Group l(GF) : Samples bonded to dentin surface with Fuji II LC after 10% polyacrylic acid treatment. Group 2(BGF) : Samples bonded to dentin surface with Fuji II LC after 10% polyacrylic acid treatment and blood contamination. Group 3(MD) : Samples bonded to dentin surface with Dyract after 10% maleic acid treatment. Group 4(BMD) : Samples bonded to dentin surface with Dyract after 10% maleic acid treatment and blood contamination. Group 5(PV) : Samples bonded to dentin surface with Variglass VLC after 10% phosphoric acid treatment. Group 6(BPV) : Samples bonded-to dentin surface with Variglass VLC after 10% phosphoric acid treatment and blood contamination. Group 1,3 and 5 were classified into the control groups, while group 2,4 and 6 were classified into the experimental groups. Each group contained 30 samples. After 24 hours water storage at $37^{\circ}C$, all smples were subjected to a shear load to fracture at a cross head speed of 1.0 mm/min with Instron universal testing machine(No. 4467). Debonded surfaces were observed under Scanning Electron Microscope(Hitachi S-2300) at 20kvp. The data were evaluated statistically at the 95% confidence level with Student's t-test. The following results obtained; 1. Shear bond strengths were higher in the control groups(1,3,5 group) than in the experimental groups(2,4,6 group). 2. The shear bond strength of group 5(PV) was the highest in the control groups, and the group 5 was significantly higher than the group l(GF) on the shear bond strength. 3. The group 4(BMD) was the highest on the shear bond strength, and the group 2(BGF) was the lowest in the experimental groups. The group 4(BMD) and 6(BPV) showed a significant difference with the group 2 on the shear bond strength. 4. All the groups showed an adhesive-cohesive failure. except the group 2(BGF) showing adhesive failure.
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