• Title/Summary/Keyword: Storage Function

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Ultrastructural Differentiation of the Vacuole in Mesophyll Tissues of Orostachys (바위솔속 엽육조직 세포 내 액포의 미세구조 분화 양상)

  • Kim, In-Sun
    • Applied Microscopy
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    • v.39 no.4
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    • pp.333-340
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    • 2009
  • In the present study, ultrastructural features of the mesophyll tissue have been investigated in Crassulacean acid metabolism (CAM)-performing succulent Orostachys. A large central vacuole and numerous small vacuoles in the peripheral cytoplasm were characterized at the subcellular level in both developing and mature mesophyll cells. The most notable feature was the invagination of vacuolar membranes into the secondary vacuoles or multivesicular bodies. In many cases, tens of single, membrane-bound secondary vacuoles of various sizes were found to be formed within the central vacuole. multivesicular bodies containing numerous small vesicles were also distributed in the cytoplasm but were better developed within the central vacuole. Occasionally, electron-dense prevacuolar compartments, directly attached to structures appearing to be small vacuoles, were also detected in the cytoplasm. One or more huge central vacuoles were frequently observed in cells undergoing differentiation and maturation. Consistent with the known occurrence of morphologically distinct vacuoles within different tissues, two types of vacuoles, one representing lytic vacuoles and the other, most likely protein storage vacuoles, were noted frequently within Orostachys mesophyll. The two types coexisted in mature vegetative cells but did not merge during the study. Nevertheless, the coexistence of two distinct vacuole types in maturing cells implies the presence of more than one mechanism for vacuolar solute sorting in these species. The vacuolar membrane is known to be unique among the intracellular compartments for having different channels and/or pumps to maintain its function. In CAM plants, the vacuole is a very important organelle that regulates malic acid diurnal fluctuation to a large extent. The membrane invagination seen in Orostachys mesophyll likely plays a significant role in survival under the physiological drought conditions in which these Orostachys occur; by increasing to such a large vacuolar volume, the mesophyll cells are able to retain enormous amounts of acid when needed. Furthermore, the mesophyll cells are able to attain their large sizes with less energy expenditure in order to regulate the large degree of diurnal fluctuation of organic acid that occurs within the vacuoles of Orostachys.

Shear Bond Strength of Composite Resin ($TESCERA^{TM}$ ATL) Veneering on Zirconia Surface with Various Surface Treatments (지르코니아의 표면처리 방법에 따른 압축강화형 복합레진 ($TESCERA^{TM}$ ATL)전장의 결합강도)

  • Park, Soo-Jeong;Lee, Richard Sung-Bok;Lee, Suk-Won;Ahn, Su-Jin;Lim, Ho-Nam
    • Journal of Dental Rehabilitation and Applied Science
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    • v.27 no.1
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    • pp.1-13
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    • 2011
  • The aim of this study was to evaluate shear bond strength of pressed reinforced composite resin ($TESCERA^{TM}$ ATL) veneering to zirconia with various surface treatments. Forty sintered zirconia specimens and forty pockmarked zirconia specimens were fabricated. All the materials were categorized as Group 1 (Control : porcelain veneering on zirconia surface), Group 2 ( $TESCERA^{TM}$ ATL dentine veneering after bonding agent application on zirconia surface), Group 3 ($TESCERA^{TM}$ ATL dentine veneering on pockmarked zirconia surface), Group 4 ($TESCERA^{TM}$ ATL dentine veneering after bonding agent application on pockmarked zirconia surface), Group 5 (Thermocycling on Group 1), Group 6 (Thermocycling on Group 2), Group 7 (Thermocycling on Group 3), and Group 8 (Thermocycling on Group 4). SBS(Shear bond strength) of 8 groups was determined with an Instron Universal Testing Machine. Also fractured surface of specimens were observed with a scanning electron microscope. There were no significant differences in the initial SBS between Group 1(control group), Group 3, and Group 4. (p>0.05) Group 2 presented the lowest SBS values. There was a no significant difference between just as 24hour water storage and simulated aging on pockmarked zirconia groups. (p>0.05) A formation of pockmarked irregularities on zirconia surface as mechanically pitted surface was reliable method for establishing a stronger bond between $TESCERA^{TM}$ ATL and zirconia-based material.

Effect on Shear Strength of Ceramic Surface Treatment Materials and Three Resin Cements to IPS Empress 2 (표면 처리재와 레진 시멘트가 IPS Empress 2의 전단결합강도에 미치는 영향)

  • Yae, Sun-Hae;Lee, Kyubok;Lee, Cheong-Hee
    • Journal of Dental Rehabilitation and Applied Science
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    • v.18 no.3
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    • pp.157-170
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    • 2002
  • The purpose of this study is to analyze the shear bond strength according to kinds of surface treatment agents and resin cements after acid etching of IPS Empress 2. For this purpose, test groups were classified into silane-treated bonding groups, silica-coated group and Targis link applied group. Then, nine bonding groups in total, each three groups per kind, were prepared by using three kinds of resin cements-Panavia F, Variolink II and Rely-X ARC, and thirty test specimens per group were prepared. To examine any changes in the oral environment, the shear bond strength of each test specimen was measured after dipping test for 24 hours and for five weeks, respectively, in distilled water at $37^{\circ}C$ and performing heat cycle 10,000 times in total, each 2,000 times per week, during a five weeks of dipping, under the condition similar to the oral environment. The bond failure modes were also observed by means of a scanning electron microscope. The results are summarized as follows 1. Statically significant differences between the surface conditioning materials were observed. The shear strength of the silane treatment was the highest of all three types of surface treatments(p<0.001). 2. Shear strengths varied significantly for different types of resin luting agents. But bond strength of Targis link surface treatments were not significantly different(p<0.05). 3. No significant difference of bonding strengths was found between storage time conditions(24 hours and 5 weeks). Only group II, IV, VII, IX were significantly different(p<0.05). 4. After thermocycling, the shear bond strengths of all groups were significantly decreased (p<0.05). Group III, V, VI were no significantly different. 5. On the SEM observation of fractured surfaces, all groups were shown complex failure.

Throughfall, Stemflow and Interception Loss at Pinus taeda and Pinus densiflora stands (테다소나무림과 소나무림에서의 수관통과우량(樹冠通過雨量), 수간유하우량(樹幹流下雨量) 및 차단손실우량(遮斷損失雨量))

  • Min, Hong-Jin;Woo, Bo-Myeong
    • Journal of Korean Society of Forest Science
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    • v.84 no.4
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    • pp.502-516
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    • 1995
  • The objective of this study was to estimate throughfall, stemflow, interception loss and net rainfall in relation to rainfall interception, and to understand the factors affecting interception process at Pinus taeda stand and Pinus densiflora stand in the Research Forests of Seoul National University, located in Choosan, Kwangyang, Chollanamdo. 1. The gross rainfall during the period of field observation was 3,107.6mm(average 1,035.9mm/year). Most of the daily rainfall intensity was under 30mm, which was 90% in 1992, 81% in 1993 and 88% in 1994. 2. In this study the throughfall, stemflow, interception loss and net rainfall were expressed separately as a function of gross rainfall. The overall throughfall collected during the period of field observation was 2,432.5mm(78.3%) at Pinus taeda stand and 2,699.6mm at Pinus densiflora stand, out of total rainfall of 3107.6mm. The canopy storage capacity, which was determined by the prediction equation between gross rainfall and throughfall was 1.1mm at Pinus taeda stand and 1.3mm at Pinus densiflora stand. 3. The sums of stemflow from measurement of total rainfall at Pinus taeda stand and Pinus densiflora stand was 227.3mm(7.3%) and 62.7mm(2.0%), respectively. The minimum rainfall causing stemflow was estimated as 7.2mm at Pinus taeda stand and 1.9mm at Pinus densiflora stand. 4. Interception loss accounted for 447.8mm(14.4%) at Pinus taeda stand and 345.3mm(11.1%) at Pinus densiflorra stand. 5. Net rainfall was 2,659.8mm(85.6%) at Pinus taeda stand and 2,762.3mm(88.9%) at Pinus densiflora stand. 6. The rates of throughfall and stemflow increased with increasing the gross rainfall. However, the amounts of throughfall and the stemflow were constant above 30mm at Pinus taeda stand and 50mm at Pinus densiflora stand. The rates of interception loss decreased with increasing the gross rainfall. However, the amount of interception loss was constant above 50mm at Pinus taeda stand and Pinus densiflora stand.

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Mathematical Models to Predict Staphylococcus aureus Growth on Processed Cheeses

  • Kim, Kyungmi;Lee, Heeyoung;Moon, Jinsan;Kim, Youngjo;Heo, Eunjeong;Park, Hyunjung;Yoon, Yohan
    • Journal of Food Hygiene and Safety
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    • v.28 no.3
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    • pp.217-221
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    • 2013
  • This study developed predictive models for the kinetic behavior of Staphylococcus aureus on processed cheeses. Mozzarella slice cheese and cheddar slice cheese were inoculated with 0.1 ml of a S. aureus strain mixture (ATCC13565, ATCC14458, ATCC23235, ATCC27664, and NCCP10826). The inoculated samples were then stored at $4^{\circ}C$ (1440 h), $15^{\circ}C$ (288 h), $25^{\circ}C$ (72 h), and $30^{\circ}C$ (48 h), and the growth of all bacteria and of S. aureus were enumerated on tryptic soy agar and mannitol salt agar, respectively. The Baranyi model was fitted to the growth data of S. aureus to calculate growth rate (${\mu}_{max}$; ${\log}CFU{\cdot}g^{-1}{\cdot}h^{-1}$), lag phase duration (LPD; h), lower asymptote (log CFU/g), and upper asymptote (log CFU/g). The growth parameters were further analyzed using the square root model as a function of temperature. The model performance was validated with observed data, and the root mean square error (RMSE) was calculated. At $4^{\circ}C$, S. aureus cell growth was not observed on either processed cheese, but S. aureus growth on the mozzarella and cheddar cheeses was observed at $15^{\circ}C$, $25^{\circ}C$, and $30^{\circ}C$. The ${\mu}_{max}$ values increased, but LPD values decreased as storage temperature increased. In addition, the developed models showed acceptable performance (RMSE = 0.3500-0.5344). This result indicates that the developed kinetic model should be useful in describing the growth pattern of S. aureus in processed cheeses.

Drainage Performance of Various Subsurface Drain Materials- (배수개선공법개발에 관한 연구(I) -각종 지하배수용 암거재료의 배수성능-)

  • 김철회;이근후;유시조;서원명
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.21 no.3
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    • pp.104-120
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    • 1979
  • I. Title of the Study Studies on the Development of Improved Subsurface Drainage Methods. -Drainage Performance of Various Subsurface Drain Materials- II. Object of the Study Studies were carried out to select the drain material having the highest performance of drainage; And to develop the water budget model which is necessary for the planning of the drainage project and the establishment of water management standards in the water-logged paddy field. III. Content and Scope of the Study 1. The experiment was carried out in the laboratory by using a sand tank model. The drainage performance of various drain materials was compared evaluated. 2. A water budget model was established. Various parameters necessary for the model were investigated by analyzing existing data and measured data from the experimental field. The adaptability of the model was evaluated by comparing the estimated values to the field data. IV. Results and Recommendations 1. A corrugated tube enveloped with gravel or mat showed the highest drainage performance among the eight materials submmitted for the experiment. 2. The drainage performance of the long cement tile(50 cm long) was higher than that of the short cement tile(25 cm long). 3. Rice bran was superior to gravel in its' drain performance. 4. No difference was shown between a grave envelope and a P.V.C. wool mat in their performance of drainage. Continues investigation is needed to clarify the envelope performance. 5. All the results described above were obtained from the laboratory tests. A field test is recommended to confirm the results obtained. 6. As a water balance model of a given soil profile, the soil moisture depletion D, could be represented as follows; $$D=\Sigma\limit_{t=1}^{n}(Et-R_{\ell}-I+W_d)..........(17)$$ 7. Among the various empirical formulae for potential evapotranspiration, Penman's formular was best fit to the data observed with the evaporation pans in Jinju area. High degree of positive correlation between Penman;s predicted data and observed data was confirmed. The regression equation was Y=1.4X-22.86, where Y represents evaporation rate from small pan, in mm/100 days, and X represents potential evapotranspiration rate estimated by Penman's formular. The coefficient of correlation was r=0.94.** 8. To estimate evapotranspiration in the field, the consumptive use coefficient, Kc, was introduced. Kc was defined by the function of the characteristics of the crop soil as follows; $Kc=Kco{\cdot}Ka+Ks..........(20)$ where, Kco, Ka ans Ks represents the crop coefficient, the soil moisture coefficient, and the correction coefficient, respectively. The value of Kco and Ka was obtained from the Fig.16 and the Fig.17, respectively. And, if $Kco{\cdot}Ka{\geq}1.0,$ then Ks=0, otherwise, Ks value was estimated by using the relation; $Ks=1-Kco{\cdot}Ka$. 9. Into type formular, $r_t=\frac{R_{24}}{24}(\frac{b}{\sqrt{t}+a})$, was the best fit one to estimate the probable rainfall intensity when daily rainfall and rainfall durations are given as input data, The coefficient a and b are shown on the Table 16. 10. Japanese type formular, $I_t=\frac{b}{\sqrt{t}+a}$, was the best fit one to estimate the probable rainfall intensity when the rainfall duration only was given. The coefficient a and b are shown on the Table 17. 11. Effective rainfall, Re, was estimated by using following relationships; Re=D, if $R-D\geq}0$, otherwise, Re=R. 12. The difference of rainfall amount from soil moisture depletion was considered as the amount of drainage required. In this case, when Wd=O, Equation 24 was used, otherwise two to three days of lag time was considered and correction was made by use of storage coefficient. 13. To evaluate the model, measured data and estimated data was compared, and relative error was computed. 5.5 percent The relative error was 5.5 percent. 14. By considering the water budget in Jinju area, it was shown that the evaporation amount was greater than the rainfall during period of October to March in next year. This was the behind reasonning that the improvement of surface drainage system is needed in Jinju area.

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A Study on Establishing the presidential Library through Elevating the Status of the Government Archives and Records Service (대통령기록관의 설립 및 정부기록보존소의 위상에 관한 연구)

  • Kim, Sung-Soo;Suh, Hye-Ran
    • Journal of Korean Society of Archives and Records Management
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    • v.2 no.1
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    • pp.41-66
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    • 2002
  • This paper examines urgent issues of establishing the presidential library in relation to the current standing of the Government Archives and Records Service. As government records have been tremendously accumulated every year, the Government Archives and Records Service will be full within three years, which indicates that the constructing of new storage repositories is an emergent issue. In addition, presidential records are one of the most significant and influential public properties for preserving historical value and interpretation at the national level. Some serious concerns relating to the presidential library are raised for consideration. First of all, it is compulsory to preserve presidential records and to construct the presidential library. Second, the presidential library performs a variety of functions including the acquisition, preservation, access, reference, research, education, and display of presidential records as well as exhibits of public activities and administration personally and in public during the president' regime. A new presidential library should function as both of a depository and museum of presidential records through systematic arrangement and display. Thus a new repository of the Government Archives and Records Service should be co-constructed with the presidential library within the same boundary. Third, a newly constructed presidential library should be at least double- or triple- sized more than estimated in order to contain all related holdings. Fourth, to take custody of public records across the legislature, judiciary and administration, the Government Archives and Records Service should be directed by an official at the level of vice-minister and upgraded to an independent office such as "National Archives and Records Administration." Fifth, the presidential library's location and surrounding environments should be selected with careful planning.

Flavor Characteristics of Kakdugi by Radish Cultivars and Seasons (무 품종 및 계절에 따른 깍두기의 향미특성)

  • Kim, Mee-Ree;Jhee, Ok-Hwa;Yoon, Hwa-Mo;Yang, Cha-Bum
    • Korean Journal of Food Science and Technology
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    • v.28 no.4
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    • pp.762-771
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    • 1996
  • Chemical and sensory characteristics of kakdugi which was prepared with various radish cultivars and harvesting seasons were analyzed during 7-day storage. Average pH of small radish cultivar kakdugi was higher than that of large ones, and total acidity was lower in small ones. Reducing sugar content was the highest in kakdugi of autumn radish. Organic acids such as lactic, succinic and fumaric acid analyzed by GC increased until the third day of fermentation, whereas volatile isothiocyanates analyzed by GC/MS continued to decline. There was a significant difference in flavor characteristics of large radish kakdugi across seasons, in contrast to no significant difference in those of small radish kakdugi except sweet taste and reducing sugar content in which interaction existed between season and cultivar. Score of overall acceptability was higher in small radish than large ones with Dongja showing the highest score of overall acceptability. Overall acceptability of autumn Dongja kakdugi was positively correlated with radish kakdugi odor and sour odor, respectively, but negatively with total acidity, lactic acid content, sweet taste and pungency, respectively. By multiple regression analysis, overall acceptability in spring Dongja kakdugi is expressed as a function of overall acceptability = -0.1115 + 1.2519 savory taste + 1.5159 malic acid -0.0054 total isothiocyanate + ${\varepsilon}$.

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Spatio-temporal Variations in the Dynamics and Export of Large Wood in Korean Mountain Streams (우리나라 산지계류에 있어서 유목 동태의 시.공간적 다양성과 그에 따른 유출 특성)

  • Seo, Jung Il;Chun, Kun Woo;Kim, Suk Woo;Im, Sangjun
    • Journal of Korean Society of Forest Science
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    • v.101 no.3
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    • pp.333-343
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    • 2012
  • In-stream large wood (LW) has a critical impact on the geomorphic characteristics relevant to ecosystem management and disaster prevention, yet relatively little is known about variations in its dynamics and subsequent export on the watershed-scale perspective in Korea. Here we review variations in the dynamics and subsequent export of LW as a function of stream size, which is appropriate for Korean mountain streams. In upstream channels with narrow bankfull widths and low stream discharges, a massive amount of LW, resulting from forest dynamics and hillslope processes, may persist for several decades on valley floor. These pieces, however, are eventually transported during infrequent debris flows from small tributaries, as well as peak hydrology in main-stem channels. During the transport, these pieces suffer fragmentation caused by frictions with boulders, and stream bank and bed. Although infrequent, these events can be dominant processes in the export of significant amounts of LW from upstream channel networks. In downstream channels with wide bankfull widths and high stream discharges, LW is dominantly recruited by forest dynamics and bank erosion only at locations where the channel is adjacent to mature riparian forests. With the LW pieces that are supplied from the upstream, these pieces are continuously transported downstream during rainfall events. This leads to further fragmentation of the LW pieces, which increases their transportability. With decreasing stream-bed slope, these floated LW pieces, however, can be stored and form logjams at various depositional sites, which were developed by interaction between channel forms and floodplains. These pieces may decay for decades and be subsequently transported as particulate or dissolved organic materials, resulting in the limitation of LW fluvial export from the systems. However, in Korea, such depositional sites were developed in the extremely limited streams with a large dimension and no flood history for decades, and thus it does not be expected that the reduction of LW export amount, which can be caused by the long-term storage. Our review presents a generalized view of LW processing and is relevant to ecosystem management and disaster prevention for Korean mountain streams.

Field Study For The Improvement of Medication System and Method for Inpatients at General Hospital (입원환자의 투약체계와 방법의 개선을 위한 현장연구)

  • Yoo, Hyung-Sook;Kuwan, Young-Mi;Song, Mi-Sook;Kim, Hyung-Ae;Park, Kyung-Sook
    • Journal of Korean Academy of Nursing Administration
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    • v.1 no.1
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    • pp.147-211
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    • 1995
  • Medication is a kind of medical service and a therapeutic nursing function which takes large portion of nursing service and requires complicated procedures. So many different medical personnel should be involved and cooporate each other in order to accomplish medication. Medication is also a vital nursing service, So nurse feels heavy responsibi lity in that she gives medication to the patient finally, so she has much responsibility if medication error is happened. Therefore it seems very important to clarify the problem of medication system and method, and find the subculture of medication situation because it may promote nursing productivity. The study was conducted to 1. Describe and interpret medication situation. 2. Find out the problem of medication system and method and on alternatives. 3. Compare the medication system and method of hospitals which are located in Seoul with object hospital Ethnographic methodology was used to study medication situation by doing participant observation and interview of health care personnel. Ten nurses and three nurse aids were interviewed. Two residents and internists, two phamacists and two accountants were also interviewed. Data was obtained and analized according to Developmental Research Sequence introduced by Spradly. On the basis of this data the results were as follows. 1. The overall flow of medication system was devided into six stage : first, checking doctor's order : second writing doctor's order, : third, transfering slip into the related departments such as account department, pharmacy : fourth, distribution of medication from pharmacy to unit : fifth, identifing medication by nurses : and finally, medicating to the patient. Behaviorors have been under a lot of stress in that they have to do much works, especially paperworks, So too much time were needed. They also have been suffered interpersonal conflicts among health care personnel and role conflicts in the process of doing medication service. 2. In the process of checking order, the problem was that too much time was required for checking order and paperwork. The more the order changes the more the paperwork is. Nurses have been suffering difficulties in calling internist in order to get bill. Even if writing down slip for medication order is doctor's job, Sometimes nurse has been expected to write slip by doctors or nurse would write slip beacuse of two much complexities and efforts for calling doctors. If the slip were incorrect, much time complicated procedures were more required for correcting it. So delay of administering drug would be resulted consequently. Drugs were delivered from pharmacy to units by delivery agent and phamacist. But because drugs were delivered without arranging room number of patient. Nurse should rearrange drugs in order of the room number So it had made waste time and effort, and Even when emergency drugs were needed, Prompt delivery of drug was not easy because of many reasons. For nurses, it took too long in the identification of the right drug. Actually nurses have heavy burden when medication error happens because nurse is the final actor who gives medication to the patient, So every three shift nurse ought to check drugs as soon as every shift begins. That's why it took too much time due to repeated confirming procedure. When nurses had to go patient room in order to give medications, there were difficulties in watching patient until the patient take medicine correctly. So it was impossible to check every patient wheather he took medicine or not especially in hectic situation. 3. There were many hospitals in Seoul which have similar medication system and method as object hospital according to the results of questionaire. This means that many hospitals have been suffering srimilar problems which were identified in object hospital. 4. Recommendations for promoting simplification of medication system and method were the following : Redesigning of slip from two pieces of paper into one : early discharge announcement system, and slip confirming through computer and controlling of period of prescreption from one day to two or three days : designing personal drug storage box for each patient and using it. If nurses follow the recommendations, they will make medication short & simple, and also have enough time of direct nursing care 5. Even though there were many difficulties in medicating patients. Medication itself has been considered as a caring among nurses because it makes rapport between nurse and patient. So nurses had better accept medication as a portion of nusing service not a original portion of phamacist. There are some limits in this research in terms of confining to only one unit of one hospital, and treating it especially in view of nurses' aspects, So further researchs should be continnued from various kmds of viewpoints of doctors, phamacists and so on. ${\cdot\cdot\cdot}$. Especially esthnographic study of computerized medication system and method seems to be followed.

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