• Title/Summary/Keyword: Storage

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Effects of Escherichia coli Contamination on Extended Porcine Semen Parameters

  • So, Kyoung-Min;Sa, Soo-Jin;Kim, Hyo-Jin;Chung, Ki-Hwa;Jung, Byeong-Yeal;Son, Jung-Ho;Kim, In-Cheul
    • Reproductive and Developmental Biology
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    • v.35 no.4
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    • pp.479-483
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    • 2011
  • The objective of this study was to determine the effects of E. coli isolated from porcine semen on sperm viability, motility, and semen pH. Semen samples were prepared using commercial extender, $Seminark^{Pro}$ (Noahbio Tech, Korea) that did not contain antibiotics. And 4 different levels of E. coli were artificially innoculated to semen with following concentrations; 4,000 of sperms with 1 of E. coli (T1), 400 with 1 (T2), 40 with 1 (T3), and 4 with 1 (T4). Semen samples were preserved at $17^{\circ}C$ for 5 days in semen storage box until analyzed by flowcytometer. Aliquots were subjected to measure the sperm viability (Live/$Dead^{(R)}$ stain), motility (mitochondrial function), and semen acidity (pH) from day 0 (day of semen collection) to day 5. Sperm motility and viability were significantly decreased (p<0.05) on day 0 (4 hrs after preservation at $17^{\circ}C$) in T3 and T4 compared to control groups and were significantly decreased (p<0.05) in all groups from day 3. Sample pH was acidic in T3 (6.90~6.86) and T4 (6.86~6.65) from day 3 to day 5 (p<0.05). On the other hand, sample pH was maintained 7.0~7.1 in control, T1, and T2 during the experimental period. Sperm motility and viability were significantly decreased from day 0 to day 5 compared to control in samples contaminated with E. coli above a value of 40:1 ($20{\times}10^6$ sperm cells/ml : $5{\times}10^5$ cfu/ml). Even on day 1 in T4 and on day 3 in T3, semen pH was acidic probably due to the acidification of dead spermatozoa. These results suggest that E. coli contamination has a concentration-dependent detrimental effect on extended porcine semen quality.

A study on development of CRM by means of XRF analysis for fine ceramic ($BaTiO_3$) (티탄산바륨 소재의 XRF 분석용 CRM 개발에 관한 연구)

  • Kim, Young Man;Jeong, Chan Yee;Lim, Chang Ho;Song, Taek Yong;Lee, Dong Soo
    • Analytical Science and Technology
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    • v.9 no.4
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    • pp.382-391
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    • 1996
  • In this study, 12 different chemical species of fine ceramic($BaTiO_3$) were synthesized as the standard materials for the fast and accurate measurements of X-ray fluorescence spectrometry. Samples were diluted to sixteen times with the filling compound ($Li_2B_4O_7+LiBO_2$) in order to remove the matrix effect, and to get the convenient storage and homogeneity of ingredients. The matrix effects among the ingredients were corrected by the empirical coefficient method based on the Lucas-Tooth and Price model. The standard curve on 12 standard materials containing 15 elements were obtained by using X-ray fluorescence spectrometry at three different laboratories. The correlation factors of BaO, PbO, SrO, $Fe_2O_3$, $La_2O_3$, $SnO_2$, ZnO, $ZrO_2$, CaO indicated the relati vely good agreement over 0.995 among the three different laboratories. $SiO_2$ and $Al_2O_3$ showed the poor linearity because of their low fluorescence intensities.

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A Study on the Buyer's Duty to Mitigate Seller's Damages in CISG (CISG상의 매수인의 손해경감의무에 관한 고찰)

  • HA, Kang-Hun
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.66
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    • pp.1-23
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    • 2015
  • A party who relies on a breach of contract must take such measures as are reasonable in the circumstances to mitigate the loss, including loss of profit, resulting from the breach. Appropriate measures are those aimed at lessing the loss as far as reasonably possible. Such measures will typically be a resale of the goods by the seller or a cover purchase by the buyer. The measures the injured party is expected to take in order to mitigate the loss must be reasonable in the circumstances. Article 77 will be applied to the difference between the amount by which the loss should have been mitigated under Article 77. A reduction of damages is the only remedy available to the party in breach in cases covered by Article 77. If the buyer has received the goods and intends to exercise any right under the contract or this Convention to reject them, he must take such steps to preserve them as are reasonable in the circumstances. If goods dispatched to the buyer have been placed at his disposal at their destination and he exercises the right to reject them, he must take possession of them on behalf of the seller. Article 86(1) requires that the buyer manifest his intention at the moment of receipt of the goods. Article 86(2) envisages that the goods have been dispatched to the buyer and that they have been placed at his disposal at their destination. Article 87 allows him to deposit them in the warehouse of a third person. It is not necessary that the warehouse by public, or that it be a general warehouse for storage. A party who is bound to preserve the goods in accordance with articles 86 may sell them by any appropriate means taking possession of the goods or in taking them back or in paying the price or the cost of preservation. If the goods are subject to rapid deterioration or their preservation world involve unreasonable expense, a party who is bound to preserve the goods must take reasonable measures to sell them. A difference exists between paragraph Article 88 (1) which grants the right to sell, and paragraph (2 )which imposes the duty to take reasonable measures to sell the goods. According to Article 88(2), the party who wishes to sell must give notice to the other party of such intention, to the extent possible.

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The aspect and meaning of the digital world view in the anime based on myth (신화를 소재로 한 애니메이션 <고스트 메신저>에 나타난 디지털 세계관의 양상과 의미)

  • Kim, Jin-Chul
    • Cartoon and Animation Studies
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    • s.49
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    • pp.299-323
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    • 2017
  • Interests to cultural prototype as a material of cultural contents are getting more increasing nowadays. One of them is myth. The important thing when you are dealing with the myth as the material that how to convert prototype narratives. Today, digital culture is becoming one of the essential elements of mankind, that is a new sociocultural phenomenon and distinguishing differentiation from the past analogue era. In this regard, the combination of mythology and digital culture is effective strategies for transforming storytelling that can resonate with people who are not familiar with myths. puts the view both of this world and underworld, mythical characters, and various elements of the Grim Reaper into the story. And combines them with digital elements. sets the world that the transcendental soul exists as physically transferred data form. and reinterprets the traditional mythology world into the digitally operated world. Soul-Phone from this anime reflects the digital culture such as storage and transmission, and helps enjoying audiences accept the unfamiliar world of myth. And also It symbolically shows dystopian viewpoints through side effects can caused by digital technology development, such as the instrumentalization of human being, the risk of communication through digital media, and ego-loss caused by duplication, etc. In conclusion, forms a consensus with the audience through the transformation strategy of storytelling that converts the world of myth into the digital world. And it reflects the dystopian view and shows a differentiated world view from the narrative of myth.

Clinical improvement in a case of atypical infantile onset Pompe disease with enzyme replacement therapy (효소 보충 치료로 호전을 보인 비전형적 영아형 Pompe 병 1례)

  • Jeon, You Hoon;Eun, Baik-Lin;Son, Chang Sung;Lee, Dong Hwan
    • Clinical and Experimental Pediatrics
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    • v.50 no.2
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    • pp.213-217
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    • 2007
  • Pompe disease is a genetic disorder caused by a deficiency of acid ${\alpha}$-glucosidase (GAA). Infantile onset Pompe disease is uniformly lethal. Affected infants generally present in the first few months of life with hypotonia, generalized muscle weakness, and a hypertrophic cardiomyopathy, which is rapidly followed by death, usually by the age of one. The late-onset form is characterized less severe symptoms and prognosis. Therapy for Pompe disease is intended to directly address the underlying metabolic defect via intravenous infusions of recombinant human GAA to replace the missing enzyme. We report a case of atypical infantile-onset Pompe disease that presented symptoms in infancy but had less severe clinical manifestations and improved after GAA enzyme replacement ($Myozyme^{(R)}$, Genzyme Co., MA, USA) therapy. It is very important that pediatricians become aware of signs and symptoms of Pompe disease, such as a nasal voice or a waddling gait at an early stage so that these patients can benefit from appropriate GAA replacement therapy as soon as possible.

Physicochemical properties of granular formulation using Paenibacillus sp. AC-1 as a microbial fungicide (미생물 살균제 Paenibacillus sp. AC-1 입제의 물리화학적 특성)

  • Oh, Kyeong-Seok;Lee, Young-Kee;Lee, Jae-Kook;Kim, Jin-Hwa
    • The Korean Journal of Pesticide Science
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    • v.9 no.3
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    • pp.262-267
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    • 2005
  • In order to commercialize Paenibacillus sp. AC-1 and to minimize its harmful side effects, four granular formulations were prepared using AC-1 powder, adjuvant, and carrier and then their physicochemical properties of the formulations were investigated. Out of the carriers tested, the best one was talc for the formulation. Viable cells was stabilized during the formulating process. Viable cells in the granules formulated with Paenibacillus sp. AC-1 powder were stabilized at storage temperature range ($4{\sim}50^{\circ}C$) after 12 weeks. The release rate of viable cells from granules into water under a static condition were eluted over 90% in 7 hours and breakdown rates of particle were 100% in 1 day. Among the tested formulations, granular formulation comprising of 20% of Paenibacillus sp. AC-1 powder, 7% of polycarboxylate as surface active agent, 1% of sodium polyacrylate as adjuvant, the rest as carrier showed to be best.

Ecological characteristics and chemical control of Aneilema keisak Hassk. in direct-seeded rice (Oryza sativa) (벼 직파재배시 사마귀풀의 발생생태 및 화학적 방제법)

  • Park, Tae-Seon;Park, Jae-Eup;Lee, In-Yong;Ryu, Gap-Hee;Kim, Kil-Ung
    • The Korean Journal of Pesticide Science
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    • v.2 no.1
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    • pp.79-84
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    • 1998
  • This study was conducted to obtain information on occurrance ecology of Aneilema keisak Hassk. and establish its effective control system in direct-seeded rice. Optimum germination temperature and storage temperature to break dormancy were $22^{\circ}C$ and $4^{\circ}C$, respectively. Optimum content of soil moisture for germination was 35%(w/w) in clay loam soil, where recorded 85% of germination rate. In the inter-specific competition between density of Aneilema keisak Hassk. and constant rice-mixed stands, the plant height and the tiller of rice were decreased with increase of density of Aneilema keisak Hassk. Among the herbicides tested, piperophos+dimethametryn(8.2+2.2 g ai/10a) GR and. pyrazosulfuron-ethyl+mefenacet(2.1 + 105 g ai/10g) GR were found to be effective for control of Aneilema keisak Hassk., having more herbicidal effect with an earlier application.

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Analyses of Scenarios Based on a Leakage of Highly Compressed Air and Fire Anticipated in CAES (Compressed Air Energy Storage) Facility (압축공기에너지저장 시설에서 발생 가능한 압축공기 유출 및 화재 시나리오 분석)

  • Yoon, Yong-Kyun;Ju, Eun-Hye
    • Tunnel and Underground Space
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    • v.25 no.6
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    • pp.568-576
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    • 2015
  • In this study, scenarios based on the leakage of highly compressed air and fire occurrence turned out to be high risks in an operation stage of CAES facility were constructed and estimated. By combining Bernoulli equation with momentum equation, an expression to calculate an impact force of a jet flow of compressed air was derived. An impact force was found to be proportional to the square of diameter of fracture and the pressure of compressed air. Four types of fire scenarios were composed to evaluate an effects that seasonal change and location of fire source have on the spread behavior of smoke. Smoke from the fire ignited in the vicinity of CAES opening descended more quickly below the limit line of breathing than one from the fire occurred 10 m away from CAES opening, which is expected to occur due to a propagation of wave front of smoke. It was shown that a rate of smoke spread of the winter fire is faster than one of the summer fire and smoke from the winter fire spreads farther than one of the summer fire, which are dependent on the direction of air flow into access opening. Evacuation simulation indicated that the required safe evacuation time(RSET) of the summer and winter fires are 262, 670 s each.

A comparative study on ammonia emission inventory in livestock manure compost application through a foreign case study (국내외 가축분뇨 퇴액비 이용 분야 암모니아 배출량 인벤토리 비교 연구)

  • Kim, Min-Suk;Koo, Namin;Kim, Jeong-Gyu
    • Korean Journal of Environmental Biology
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    • v.38 no.1
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    • pp.71-81
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    • 2020
  • In Korea, more than 70% of the ammonia(NH3) released into the atmosphere is known to originate from livestock manure. The total emission (kg year-1) is calculated by multiplying the emission factor (kg head-1 yr-1) and the activity data (head). To improve the accuracy and reliability of the NH3 emission estimation process, an accurate account of livestock manure production, calculation of NH3 conversion and generation during the composting and liquefaction of manure, estimation of NH3 generation in the storage and transportation of manure and compost, and a comparative study of NH3 emission during the soil spreading process must be performed. Compared to the US and EU-28, in particular, the domestic emission factor is relatively even and the spatial/temporal scale is not broken down sufficiently to reflect the domestic situation. As a way to improve the accuracy and expertise of estimating NH3 emission factors, a 'dynamic chamber-capture system' can be utilized, which allows complex considerations of compost, liquid manure, soil, and climate characteristics. By reviewing and comparing the data related to domestic and foreign NH3 emission, we identified shortcomings in the current domestic system and the directions to be taken and suggested a chamber system that could estimate NH3 emission flux. It is also necessary to establish a methodology for mesocosm systems in the field, in addition to indoor chamber systems, to be linked with practical policies, such as the calculation of new emission factors for missing sources.

Assessment on the Forest Conservation Value Considering Forest Ecosystem Services - The case of Gapyung-gun - (산림 생태계 서비스를 고려한 산림 보전가치 평가 - 가평군을 대상으로 -)

  • Jin, Yihua;Jeong, Seunggyu;Jeong, Seulgi;Lee, Dongkun
    • Journal of Environmental Impact Assessment
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    • v.24 no.5
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    • pp.420-431
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    • 2015
  • As biodiversity and climate change have become main issues in recent times, the role of the forest ecosystem has been more important and forest conservation has been highlighted. The purpose of this study is to estimate forest area with high conservation values in Gapyung-gun by considering forest ecosystem services. The indicators of biodiversity, climate regulation, and water regulation were selected for assessment in this study. To assess biodiversity, habitat structural features and distribution characteristics of species were analyzed. Climate regulation and water regulation were assessed through analysis of carbon absorption volume and water storage. The result showed that, 50.1% of the forests in Gapyung-gun had high conservation values. The results were verified by comparing them with distribution tendencies of other environmental maps, which represent forest ecological values, and showed similar distribution tendencies. The study was conducted on only Gapyung-gun in Korea; however, the methods used in this study could be utilized for assessment of other areas to identify forests with high conservation values.