• Asian Journal of Turfgrass Science
• /
• v.15 no.2
• /
• pp.77-86
• /
• 2001

The experiments were carried out to investigate effect of shading rare on variegata appearance and leaf growth of variegated liriope (Liriope platyphylla Wang et Tang variegata Hort.) The plant was grown under four different light intensities such as 0(natural light intensity), 25, 50 and 75% shading conditions. Leaf variegata appearance was better in the light than in the shade. Leaf showed good growth at 0, 25% shading treatment, average leaf width and area of yellow part were highest at 0% shading treatment. As increased shading rate, number of stomate per unit area decreased. Total chlorophyll of the whole leaf and green part were reduced by increasing shading rate, while yellow part showed oppositely. In the epidermis, cell size of 75% shading treatment showed larger than those of 0% shading treatment.

• Korean Journal of Plant Resources
• /
• v.12 no.1
• /
• pp.10-19
• /
• 1999

The water status of Pinus densiflora and P. koraiensis was observed after the treatment of simulated acid rain for every month August 1997 to March 1998. Both of the species showed increase of dry weight, water saturation deficit and epidermis transpiration after treatment of simulated acid rain (pH 3.5) for first three months. On the contrary, water content was decreased in both the species after three month of simulated acid rain treatment. The effect of simulated acid rain, however, became weaker after that and no big difference was observed after seven month of treatment. Water potential of the leaf of both species became lower than that of the control(pH 5.6) after seven month treatment of simulated acid rain. The cuticle layers of the epidermis and florins ring were damaged seriously and the size of the gap between stomata became bigger after the treatment of simulated acid rain.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.26 no.1
• /
• pp.45-50
• /
• 1981

Diurnal changes in leaf stomatal resistances were measured on leaf positions and both surfaces to investigate the stomatal response to irradiance in wheat, var. Chokwang and barley, var. Dongbori 1. Stomatal frequency and size were also determined to explain the control mechanism of gas exchanges in two species. The leaf diffusive resistances of two species decreased, as the sun rose, to minimum at 10 to 11 o'clock a.m. and increased gradually in the afternoon, even faster at sunset. As the adaxial irradiance increased, stomatal resistances decreased sensitively in the range of 30uEm$^{-2}$ㆍsec$^{-1}$ to 150uEm$^{-2}$ㆍsec$^{-1}$ quantum flux density. The stomatal opening of the abaxial surface began at lower irradiance and was completed earlier than the adaxial surface. The adaxial irradiances decreased in order of leaf position, flag, the 2nd, the 3rd leaf, and the stomatal resistances increased in the same order. Even under the same irradiance, the stomatal resistance of lower leaves were higher than those of upper leaves. The stomatal frequencies of lower leaves were less, but the stomatal size was greater than those of upper leaves. Consequently, the relative leaf area occupied by stomatal pores were constant among leaf positions in two species.

• Korean Journal of Plant Resources
• /
• v.14 no.3
• /
• pp.188-195
• /
• 2001

The anatomical characteristics of rhizome, lateral root, stem, leaf, petiole, ovary and leaf epidermis about Atractylodes japonica Koidz. ex Kitam. and A. macrocephala Koidz. were investigated to estimate these taxonomic values. The cross sections of stem and ovary were very similar to two species, therefore these characters were not useful for delimitation of two species, but the size and distribution numbers per 100${\mu}{\textrm}{m}$$^2$of oil cavites in rhizome, the shape of leaf and petiole in cross section, the size of leaf epidermal cell and distribution numbers per 100 ${\mu}{\textrm}{m}$$^2$of stomata were differ from two species, therefore these were thought to be useful characters for delimitation of two species.

• Korean Journal of Breeding Science
• /
• v.42 no.5
• /
• pp.431-438
• /
• 2010

Anthers of sweet pepper $F_1$ cultivar 'Special' were cultured on Dumas De Vaulx (C medium), supplemented with $0.1mgL^{-1}$ 2, 4-D and $0.1mg{\cdot}L^{-1}$ kinetin with 3% sucrose, and 0.32% phytagel. The calluses obtained were further sub-cultured on Murashige and Skoog (MS) medium without growth regulators for regeneration. Regenerated plantlets were grown in plastic pots under plastic house and characterized their cytological and morphological characters in spring, 2008. Twenty percent plantlets were identified as haploid plants after chromosome and ploidy analysis. Haploid plants contained 12 chromosomes, high stomatal density with small stomatal length as compared to diploid plants. Stomatal length in haploids was 23.3% smaller than diploids. Haploid plants were characterized as small leaf and petiole size, poor vigor, thin stem and short plant height, short internodes and small flower buds, fruit size and fruit weight as compared to diploid plants and most of the haploid fruits were seedless. SP55, SP62, SP68, SP72 and SP77 are found high yielding double haploids with high total soluble content (8.6, 8.7, 9.2, 9.1 and $9.8^{\circ}Brix$, respectively) and desirable fruit shape, and recommended them to exploit as inbred lines for heterosis breeding.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.29 no.4
• /
• pp.307-314
• /
• 1984

One hundred winter wheat cultivars obtained from the world collections in Korea were planted in 1982. Stomatal frequency of flag leaf of these cultivars were measured, and among these 21 cultivars which showed different stomatal frequency were selected to investigate the relationship of their stomatal frequencies and other characters such as culm length, heading date and characters related to leaf. Stomatal frequency of flag leaf of 100 cultivars different significantly, ranging from 51 to 90 stomata mm$^{-2}$ for the adaxial and 35 to 65 for the abaxial surfaces of leaf. Stomatal frequency was always greater on the adaxial than on the abaxial surface. Mean ratios (adaxial/abaxial) were 1.5 for the flag leaf and 1.3 for the from second to fouth leaves. Among 21 cultivars examined, stomatal size which measured guard cell length varied from 38.5 to 46.6$\mu$ for adaxial surface and from 39.4 to 49.4$\mu$ for abaxial. A negative correlation was obtained between stomatal frequency and its size. Stomatal frequency decreased from flag (L$_1$) to lower leaves, progresively. Stomatal frequency on L$_1$ sheath was compared to those on L$_3$ and L$_4$ abaxial surface of leaf. More stomatal frequency on top and center of adaxial surface showed than that on basal part of a leaf but the abaxial surface resulted in reverse of adaxial one. Cultivars with high stomatal frequency on flag leaf showed higher stomatal frequency on the other leaves and sheaths. Positive correlation between stomatal frequency and other characters such as, culm length, heading date and chlorophyll content showed statistical significance, whearas it revealed the negative correlation of stomatal frequency with leaf weight, leaf area and stomatal aperture. However, no relation appeared between stomatal frequency and leaf vein.

• Journal of Plant Biology
• /
• v.28 no.2
• /
• pp.105-118
• /
• 1985

This study was carried out to investigate the epidermal structure and types of stomata on the upper and lower surfaces of the leaves in six species and one variety belonging to Korean Crassulaceae. The shape of epidermal cells was polygonal, isodiametric, or elongated. The cell wall was thick, sinuous or deeply sinuous. The subsidiary cell wall was thin or mostly arched. The cytolith-containing cells were found in Orostachys japonicus. The distribution of stomata was more on the upper surface than on the lower surface in the leaves of O. japonicus and Sedum sarmentosum. The stomata in the other species were less on the upper surface than on the lower surface, and stomatal size was different in each species. The great majority of stomatal types was the helico-eumesogenous type. This type was subdivided into two, parahelico-eumesogenous type and diahelico-eumesogenous type by us on the basis of the angle of division of guard mother cell and the long axis of the last-formed subsidiary cells. Sometimes allelo-eumesogenous type as well as aniso-eumesogenous type was found. The tetra-eumesogenous type with four mesogenous subsidiary cells was rarely observed. It was observed that the varieties of aniso-eumesogenous type with one to three subsidiary cells and one helix of subsidiary cells were developed by the secondary division of subsidiary cells of aniso-eumesogenous stoma. The compound shape of helico-eumesogenous stomata was found. Five new kinds of stomatal types were observed as follows; the helico-tetra-eumesogenous type, the coallelohelico-eumesogenous type, the cohelico-allelo-eumesogenous type, the duplotetra-eumesogenous type, and the aniso-euperigenous type. These types were reported here in the vascular plants for the first time.

• Korean Journal of Plant Taxonomy
• /
• v.47 no.3
• /
• pp.222-235
• /
• 2017

A comparative study of the leaf epidermal micromorphology in the tribe Neillieae (Neillia: 4 species, 4 varieties; Physocarpus: 5 species; Stephanandra: 2 species) was carried out using scanning electron microscopy in order to evaluate the taxonomic and systematic implications of these characteristics. The leaves of the genera Neillia and Stephanandra were hypostomatic, whereas those of P. monogynus, P. opulifolius were amphistomatic. The range of the size of the stomata is $12.02-34.39{\times}10.76-27.13{\mu}m$; the smallest was found in N. thyrsiflora (average $13.98{\times}12.43{\mu}m$; $L{\times}W$), while the largest was measured in N. gracilis (average $26.82{\times}20.67{\mu}m$; $L{\times}W$). Paracytic stomata complexes are only found in N. affinis, and the anomocytic type was most commonly found. The papillate epidermal cell type was only observed on the abaxial surfaces of P. insularis. Platelet epicuticular waxes were found on the adaxial surfaces of N. affinis and S. tanakae. Four types (unicellular non-glandular, two- to five-armed, stellate, and glandular) of trichomes were found on the leaves. Stellates were observed in all species of Physocarpus except for P. insularis. Consequently, leaf epidermal micromorphological characteristics (e.g., the presence of papillate epidermal cells and stellate, and stomata complexes) may have high taxonomic and systematic value in Neillieae. Our results strongly support previous molecular phylogenetic and palynological hypotheses that Stephanandra and Neillia are a single genus and that Physocarpus insularis should be considered as a member of Spiraea.

• Journal of Sericultural and Entomological Science
• /
• v.7
• /
• pp.1-26
• /
• 1967

In order to develop the standards for the measurement of drought resistance in mulberry trees (Morus genus) the varietal differences of drought resistance were measured for 30 mulberry varieties, and the relationships between the drought resistance and the histological and physiological characteristics of mulberry leaves were investigated. The results were summarized as follows; 1. It is reasonable to use the drought resistance ratio, expressed by D/D'＊100, for the standard of drought resistance measurement for mulberry tree as a perennial tree crop. Where: D stands for growth amount(shoot length) in the plot of dry treatment, at the end of treatment. D' stands for an expected value of D which is expressed by B＊C/A. Here, A is the growth amount of wet treatment plot at the beginning of treatment, B is the growth amount of dry treatment plot at the beginning of treatment, and C is the growth amount of wet treatment plot at the end of treatment. 2. The results obtained from the application of above formula showed that the varieties Cadaneo, Tahozosaeng, Yongchunchuwu, Kaeryang suban. and Kabsun were highly resistant to drought and the varieties Jukmok, Shipyung, Sobun, Kaeryangzosaeng shipmoonza and Chungagokyo were highly susceptible. 3. Among leaf tissues. the rate of inter-cellular space showed the highest relationship with drought resistance. The correlation coefficient calculated (r=0.4153) was highly significant. Other leaf tissues such as epidermis and palisade showed no significant correlations with drought resistance. 4. The size and density of stomata were correlated to drought resistance. That is: Correlation between drought resistance and size of stomata(length ${\times}$ width)......r= -0. 3253(signif. at 5%) density(No. of stomata/l$\textrm{mm}^2$......r= +0.5047(signif. at 1%)

• Journal of Plant Biotechnology
• /
• v.33 no.2
• /
• pp.117-122
• /
• 2006

Comparative studies on medium supply in bioreactors (raft, immersion and ebb and flood) have revealed that multiplication and growth of Alocasia Amazonica were greatest in the raft system, while lowest in ebb and flood system. In the raft system, the basal part of the shoots was continuously in contact with medium, which enabled a constant uptake of nutrients as well as aeration to the explants. The number and the size of leaf stomata were higher in the raft system compared with immersion and ebb&flood system. In the immersion system, plantlets were deformed and epidermal cells in leaves were irregular with a large intercellular space. The results suggested that the medium supply should be controlled properly to maintain normal and healthy plantlets during liquid cultures in bioreactors Which affects morphology and physiology Of the plantlets.