• 한국작물학회지
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• 제22권2호
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• pp.112-120
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• 1977

본 시험은 제주도에서 stevia (Stevia rebaudian a Bertoni M.)의 효과적인 번식, 월동방법 및 재식거리에 따른 생육과 적응성을 알고져 실시 하였던바 몇가지 얻어진 결과를 요약하면 다음과 같다. 1) 식물생장조절제처리에 의한 발근율은 I.B.A=atonic(equation omitted)N.A.A>tachigalen>rooton>centre의 순위였고 발근수는 I.B.A>N.A.A>rooton>tachigalen>atonic>control 순위였으며 식장은 I.B.A(equation omitted)N.A.A>rooton>tachigalen>atonic>control 순위였다. 2) 상토별 효과에서는 발근율이 송이>심토>냇모래 (천사)>모래의 순위였고 발근수는 송이(equation omitted)모래>심토(equation omitted)냇모래의 순위였으며 근장은 모래>송이>냇모래>심토의 순위였다. 3) 월동효과는 polyethylene film tunnel이 가장효과가 좋았고 다음으로 polyethylene 피복이 효과적이었으며 볏짚과 왕겨피복은 재(회) 피복이나 무처리에 비해서는 효과적이나 지온상승에는 효과가 크지 못하였다. 4) 재식거리별 효과는 밀식 할수록 전체중이나 건엽중도 많았다. 5) 초장신장은 기온이 높을때 비례적으로 증가하며 7월하순부터 8월하순에 걸쳐 신장량은 급진되었다.

• 한국식품영양학회지
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• 제21권2호
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• pp.190-196
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• 2008

This study was performed to aid the development of a reduced-calorie ssanghwa beverage, by using substitutes for high fructose com syrup(HFCS). The relative sweetness levels of HFCS, aspartame, acesulfame-K, and glucosyl stevia solutions were examined in comparison to a 10% sucrose solution in a binary solution model. And the sensory properties of ssanghwa beverages containing aspartame, acesulfame-K, and glucosyl stevia were evaluated at the equi-sweetness to HFCS. In the binary solution model, the relative sweetness of HFCS to sucrose was 0.8, while the values for aspartame, acesulfame-K, and glucosyl stevia were 140, 170, and 100, respectively. Sweet taste and sweet after taste were not significantly different between the HFCS, aspartame, acesulfame-K, and glucosyl stevia solutions. On the other hand, bitter taste, first taste, and overall eating quality were significantly different between the HFCS and aspartame solutions and between the acesulfame-K and glucosyl stevia solutions. Finally, the ssanghwa beverages sweetened with HFCS, acesulfame-K, and aspartame only had slight differences in sensory properties. However, the sensory properties of the beverages sweetened with HFCS and glucosyl stevia, respectively, were significantly different.

• Journal of Plant Biotechnology
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• 제30권2호
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• pp.151-154
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• 2003

This study was conducted to determine the optimal concentrations of plant hormones (2,4-D, picloram, dicamba, NAA, kinetin) and the suitable explants among seeds, hypocotyl, and cotyledons on calls formation and plant regeneration of stevia(Stevia rebaudiana Bertoni). The frequency of cellus formation was higher in the young leaf-explants then the older ones, and in the seeds then the hypocotyls and cotyledons on MS medium with 1mg/L 2,4-D. After transfer of seed-derived stevia callus producing embryogenic callus on plant-regeneration medium, the frequency of plant regeneration from callus was 23.8% in MS medium with 1mg/L NAA and 3mg/L kinetin.

• 대한영양사협회학술지
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• 제23권1호
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• pp.14-26
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• 2017

The purpose of this study was to determine the optimal recipe of rice cookies with two different amounts of Stevia rebaudiana leaf and grape seed oil, using a central composite design (CCD). In addition, mixing conditions of rice cookies were optimized by sensory evaluation and mechanical and physicochemical analysis using response surface methodology (RSM). RSM was used to obtain 10 experimental points (including two replicates of Stevia rebaudiana leaf and Grape seed oil), and the formulation of Stevia rebaudiana leaf added rice cookies was optimized using rheology. The results of mechanical and physicochemical analysis showed significant values for lightness, redness, yellowness, hardness, spread factor, loss rate, leavening rate, sweetness, moisture, pH, and density (P<0.001), results of the sensory evaluation showed significant values for color, flavor, taste, texture, appearance, and overall quality (P<0.05). As a results, optimal sensory ratio was found to be 1.98 g of Stevia rebaudiana leaf and 37.94 g of Grape seed oil.

• 한국식품영양과학회지
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• 제39권2호
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• pp.313-318
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• 2010

본 연구는 추출처리 방법별 스테비아 추출물의 항산화능을 비교하기 위하여 수행되었다. 스테비아 추출물은 열수(HWE), 발효(FHWE) 및 감압(VE)추출의 처리과정을 통하여 추출되었다. DPPH 라디칼 소거능과 환원력(FRAP) 및 지방산화 억제능을 통하여 알아본 항산화능의 평가는 감압처리되어 추출된 스테비아 추출물(VE)의 경우 그 활성이 가장 높게 관찰되었으며, 3가지 추출물 모두, 동일 농도에서 stevia의 주요한 활성성분인 stevioside와 rebaudioside보다 항산화활성이 월등히 높게 관찰되었다. 이러한 항산화능의 증가에 관한 이유를 알아보기 위하여 총 phenolic compounds의 함량을 측정한 결과 감압(VE) 추출물에서 유의적으로 높게 관찰되었다. 따라서 감압(VE) 처리 방법은 항산화 활성이 증진된 스테비아 추출물을 얻기 위한 효과적인 처리공정이라 사료된다.

• 생약학회지
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• 제9권3호
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• pp.149-156
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• 1978

The result of the study about the cultivation, the constituents, the sweetenity, and the development of preparations of Stevia and Hydrangea are as follows: 1. Hydrangea thrived at $200{\sim}1,400m$ above the sea-level, and the good ones were brought up at $600{\sim}1,200m$. 2. The good ones which belonged to the genera of Hydrangea were produced in the damp, dark and cold place. 3. The growth and cultivated yield of Stevia was not significantly influenced by the acidity of the soil. 4. The best season was May and June for propagation of Stevia by cutting method. 5. The growth rate of Stevia and Hydrangea was really good in the sandy fertile soil, but in the mucotic soil it was not suitable. 6. The extraction and separation of phyllodulcin by solvent had many difficulties, because it was very soluble in water, ethanol, ether, petroleum ether, acetone and benzene, etc. 7. The solubility of stevioside on the solvent was as follows: It was very soluble in water and methanol, slightly soluble in ethanol and acetone, and insoluble in ether, petroleum ether and chloroform. 8. The alkaloid reaction by Mayer reagent in Hydrangea extract was positive. 9. The ashification rate of Stevia was 8.66% to 8.72% and that of Hydrangea 17.02% to 17.04%. 10. The tannin of Stevia leaf was 7.80% to 7.88% and its of Hydrangea decreased 9.46% to 6.08% by fermentation. 11. The percent rates in minimum concentration-occurring sweetness in sugar, glycyrrhiza methanol extract, Hydrangea water extract, Stevia leaf water extract, decoction of Stevia's leaf, decoction of Stevia's stem were as follows: 1.2, 0.1, 0.1, 0.2, 0.4, 0.01, 0.1, 0.6. and sweetenity ratios of those were 1, 12, 12, 6, 3, 64, 12, 2, 12. It was very meaningful to develop preparations of stevia as stevioside, micronized powder, water extract, methanol extract and compound teas and in Hydrangea, water extract, methanol extract, single tea, and compound teas were less meaningful. 13. The genera of Hydrangea which is natural species in Korea was positive in phyllodulcin-identification test, but it was not available to make the sweet tea because of having a little content.

• 방사선산업학회지
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• 제6권2호
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• pp.189-197
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• 2012

This study was carried out to develop the improved useful mutants for yield or composition of stevia plants using the gamma ray or chemical mutagens treatments. The seeds of stevia 'Suwon No. 11' were irradiated up to 400 Gy of gamma ray. Chemical mutagens were treated on the seeds of the 'Suwon No. 11' using 0.07% colchicine, 10 mM sodium azide, or 10 mM NMU for various durations. The germination rate, and shoot and root growth of seedling were estimated at 30 days after gamma ray irradiation or chemical mutagen treatment, and the plant height, the number of branches, and leaf length and width were examined at 3 months after mutagenesis treatments. In the case of gamma ray treatments, the germination rate and early-stage growth were decreased as the increase of radiation dose, and the 50% lethal dose was found to be 200 Gy. the plant height was decreased as the increase of radiation dose, while the number of branches per plant and leaf length were increased. Leaf shape was modified to the relatively longer one compared to the control, which was identified more apparently at the treatments of higher than 150 Gy. In the treatment of chemical mutagens, the rate of germination and survival were decreased as the increase of incubation time. The 50% lethal dose for germination rate were identified as the conditions of the 15 hours incubation in 0.07% colchicine, the 4 hrs in 10 mM sodium azide, and the 2 hrs in 10 mM NMU, in the three chemical mutagens treatments. Chemical mutagens had no influence on shoot growth, while root growth was increased, especially as the incubation time was extended. The highest root growth occurred in the NMU treatment at 6 hrs incubation time. The plant height was decreased as the increase of incubation time in the chemical mutagens treatments. Among the chemical mutagens, NMU was the most effective to induce the mutants with long-shaped or the least lobed leaves.

• 한국자원식물학회지
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• 제32권3호
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• pp.220-227
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• 2019

Stevia rebaudiana (Asteraceae), a perennial plant, has been used as a low-calorie sweetener and is being developed as a therapeutic agent for diabetes, hypertension, myocardial diseases, and microbial infections. Despite the common use of its leaves and stem, the bioavailability of the components present in S. rebaudiana flowers, when used as ingredients of cosmetics, has not been well investigated. Herein, we investigated the antioxidative and antimelanogenic effects of an aqueous extract of S. rebaudiana flowers (Stevia-F). Total flavonoid and phenolic content in Stevia-F were determined to be $8.64{\pm}0.23mg$ of quercetin equivalents/100 g and $631.5{\pm}2.01mg$ of gallic acid equivalents/100 g, respectively. The $IC_{50}$ values of Stevia-F for reducing power, and 2,2-diphenyl-1-picryl-hydrazyl-hydrate radical, hydrogen peroxide, and nitric oxide scavenging activities were 5541.96, 131.39, 466.34, and $10.44{\mu}g/mL$, respectively. Stevia-F showed inhibitory effects on the tyrosinase ($IC_{50}=134.74{\mu}g/mL$) and ${\alpha}$-glucosidase ($IC_{50}=114.81{\mu}g/mL$) activities. No significant cytotoxicity of Stevia-F was observed in B16F10 cells, treated with up to $100{\mu}g/mL$ of the extract for 24 and 48 h (p > 0.05). Stevia-F ($1-100{\mu}g/mL$) suppressed ${\alpha}$-melanocyte stimulating hormone-induced melanin production in B16F10 cells (p < 0.05) and also inhibited the cellular tyrosinase activity (p < 0.05). Overall, our results show that Stevia-F possesses potential for inhibiting tyrosinase and ${\alpha}$-glucosidase activities and has significant antioxidant capacity. The antimelanogenic potential of Stevia-F should extend the usage of S. rebaudiana flowers in the development of skin-whitening products.

• 한국가정과학회지
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• 제9권1호
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• pp.71-80
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• 2006

To investigate the effects of the supplementation of Stevia. Rebaudian Bertoni on serum and hepatic lipid levels and enzyme activities in rat administered with ethanol chronically. Sprague-Dawley male rats were orally treated with either an AIN-76 diet(C, control), a control diet plus ethanol(E, 4g/kg bw), E plus stevia extract-1(ES1, 1ml/kg bw), or E plus stevia extract-2(ES2, 2ml/kg bw) for 7 weeks. Serum triglyceride levels were increased in the E group and were decreased in the ES 1 group. Liver triglyceride levels were decreased significantly in the ES2 groups and Total-cholesterol were decreased in the ES1, ES2 groups compared with the E group. Liver $\gamma-GTP$ levels were decreased significantly in the ES1, ES2 groups compared with the E group. In addition, we have evaluated the serum or liver carnitine levels in those groups. Liver TCNE levels were increased significantly in the ES1, ES2 compared with the E group. These results may suggest that supplementation of Stevia. Rebaudian Bertoni has effects on the recovery of chronic ethanol-related diseases.

• 한국식품저장유통학회지
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• 제11권4호
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• pp.491-495
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• 2004

천연 저 칼로리 감미료로써 stevia의 소비가 증가하고 있는 시점에서 재배 및 기능성 식품소재로 이용하기 위하여 수확시기별 특성을 조사한 결과는 다음과 같다. 가용성 고형분 함량과 추출수율은 성장 기간이 길어질수록 증가하였고, pH는 감소하는 경향을 보였다. 색도와 투과율은 큰 변화가 없었으나, 성장 시기가 길어질수록 L, a, b값은 유사한 감소 경향을 나타내었고, 갈색도는 증가하였다. 성장기간 중 stevioside와 rebaudioside A의 비율은 stevioside는 $68.97\%$에서 $61.09\%$까지 감소하고, rebaudioside A는 $20.83\%$에서 $27.52\%$까지 증가하였다. Stevia 잎의 감미성분함량은 성장함에 따라 일정한 수준으로 증가하여 최고치는 8월말이며 함량은 $12.80\%$였고, 9월말에는 함량이 $12.10\%$로 감소하는 경향을 보였다. 최고 감미도는 25 - 30배였으며 감미질은 5월말에 채취한 시료가 가장 우수하였다.